JP7372910B2 - ヒト多能性幹細胞由来の胸腺オルガノイドのインビトロ生成 - Google Patents

ヒト多能性幹細胞由来の胸腺オルガノイドのインビトロ生成 Download PDF

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JP7372910B2
JP7372910B2 JP2020516436A JP2020516436A JP7372910B2 JP 7372910 B2 JP7372910 B2 JP 7372910B2 JP 2020516436 A JP2020516436 A JP 2020516436A JP 2020516436 A JP2020516436 A JP 2020516436A JP 7372910 B2 JP7372910 B2 JP 7372910B2
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cells
thymic
vitro
organoids
keratin
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イー． ヴィズカルド、ラウル
ピー． レスティフォ、ニコラス
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    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/065—Thymocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/26—Lymph; Lymph nodes; Thymus; Spleen; Splenocytes; Thymocytes
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    • C12N5/0062—General methods for three-dimensional culture
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
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    • C12N2513/00—3D culture

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  • Health & Medical Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Pharmacology & Pharmacy (AREA)
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  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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JP2020516436A 2017-09-20 2018-09-19 ヒト多能性幹細胞由来の胸腺オルガノイドのインビトロ生成 Active JP7372910B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762560908P 2017-09-20 2017-09-20
US62/560,908 2017-09-20
PCT/US2018/051625 WO2019060336A1 (en) 2017-09-20 2018-09-19 IN VITRO GENERATION OF THYMIC ORGANOID FROM HUMAN PLURIPOTENT STEM CELLS

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JP2020534004A JP2020534004A (ja) 2020-11-26
JP2020534004A5 JP2020534004A5 (https=) 2021-11-04
JP7372910B2 true JP7372910B2 (ja) 2023-11-01

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US (1) US11898166B2 (https=)
EP (1) EP3684920A1 (https=)
JP (1) JP7372910B2 (https=)
WO (1) WO2019060336A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025150823A1 (ko) * 2024-01-11 2025-07-17 재단법인 아산사회복지재단 흉선 세포 또는 흉선 상피세포를 유효성분으로 포함하는 암 예방용 약학적 조성물

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US9719068B2 (en) 2010-05-06 2017-08-01 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
SG10201801654RA (en) 2014-05-28 2018-04-27 Childrens Hospital Med Ct Methods and systems for converting precursor cells into gastric tissues through directed differentiation
EP4553082A3 (en) 2016-11-04 2025-08-20 Children's Hospital Medical Center Liver organoid compositions and methods of making and using same
JP7248586B2 (ja) 2017-04-14 2023-03-29 チルドレンズ ホスピタル メディカル センター 複数ドナー幹細胞組成物およびそれを作製する方法
EP3694603B1 (en) 2017-10-10 2026-04-08 Children's Hospital Medical Center Esophageal tissue and/or organoid compositions and methods of making same
EP3727394A4 (en) 2017-12-21 2021-09-08 Children's Hospital Medical Center DIGITALIZED HUMAN ORGANOIDS AND METHOD OF USING THEREOF
EP4678727A3 (en) 2018-07-26 2026-03-11 Children's Hospital Medical Center Hepato-biliary-pancreatic tissues and methods of making same
CA3112026A1 (en) 2018-09-12 2020-03-19 Children's Hospital Medical Center Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
US12600943B2 (en) 2019-02-01 2026-04-14 The University Of Hong Kong Innervated organoid compositions and methods of making same
JP7740706B2 (ja) * 2019-04-01 2025-09-17 ザ・トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク 多能性幹細胞の胸腺上皮細胞および胸腺上皮前駆細胞分化を促進する方法
WO2020220040A1 (en) * 2019-04-26 2020-10-29 The Regents Of The University Of Colorado, A Body Corporate Generation of functional and patient-specific thymic tissue in vivo from induced pluripotent stem cells
US12497597B2 (en) 2019-05-31 2025-12-16 Children's Hospital Medical Center Methods of generating and expanding hematopoietic stem cells
AU2020283048A1 (en) 2019-05-31 2021-12-23 Children's Hospital Medical Center Shaped organoid compositions and methods of making same
JP7676361B2 (ja) * 2019-08-13 2025-05-14 チルドレンズ ホスピタル メディカル センター オルガノイド組成物を作製するための改良された方法
KR20220113720A (ko) * 2019-11-25 2022-08-16 앨리게니 싱어 리서치 인스티튜트 인간 전분화능 줄기 세포로부터 생체공학적으로 조작된 흉선 오가노이드
CA3179236A1 (en) * 2020-04-28 2021-11-04 The Regents Of The University Of California Methods for generating thymic cells in vitro
WO2022076751A1 (en) * 2020-10-07 2022-04-14 The Regents Of The University Of Colorado, A Body Corporate Compositions, methods and use for in vitro and in vivo generation of functional thymic tissue
GB2601793B (en) * 2020-12-10 2025-07-23 The Francis Crick Institute Ltd Thymic constructs and uses thereof
WO2023064457A1 (en) * 2021-10-15 2023-04-20 Thymmune Therapeutics, Inc. Thymic cells and methods of making
US20250332259A1 (en) * 2022-06-17 2025-10-30 Kyoto University Thymic epithelial cell production method
CN119614476A (zh) * 2024-11-13 2025-03-14 广州市第一人民医院（广州消化疾病中心、广州医科大学附属市一人民医院、华南理工大学附属第二医院） 胸腺类器官的构建方法和应用

Citations (1)

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WO2010143529A1 (ja) 2009-06-09 2010-12-16 国立大学法人名古屋大学 多能性幹細胞を胸腺上皮へ分化誘導する方法

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SG11201803419PA (en) 2015-10-30 2018-05-30 The Regents Of The Universtiy Of California Methods of generating t-cells from stem cells and immunotherapeutic methods using the t-cells

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Cited By (1)

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Publication number Priority date Publication date Assignee Title
WO2025150823A1 (ko) * 2024-01-11 2025-07-17 재단법인 아산사회복지재단 흉선 세포 또는 흉선 상피세포를 유효성분으로 포함하는 암 예방용 약학적 조성물

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US20200270571A1 (en) 2020-08-27
WO2019060336A1 (en) 2019-03-28
JP2020534004A (ja) 2020-11-26
US11898166B2 (en) 2024-02-13
EP3684920A1 (en) 2020-07-29

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