JP2020534004A5 - - Google Patents
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- JP2020534004A5 JP2020534004A5 JP2020516436A JP2020516436A JP2020534004A5 JP 2020534004 A5 JP2020534004 A5 JP 2020534004A5 JP 2020516436 A JP2020516436 A JP 2020516436A JP 2020516436 A JP2020516436 A JP 2020516436A JP 2020534004 A5 JP2020534004 A5 JP 2020534004A5
- Authority
- JP
- Japan
- Prior art keywords
- cells
- thymic
- organoid
- keratin
- vitro
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 230000002992 thymic effect Effects 0.000 claims 27
- 238000000034 method Methods 0.000 claims 24
- 210000002220 organoid Anatomy 0.000 claims 18
- 210000004027 cell Anatomy 0.000 claims 15
- 238000000338 in vitro Methods 0.000 claims 9
- 210000001744 T-lymphocyte Anatomy 0.000 claims 8
- 102000005705 Keratin-5 Human genes 0.000 claims 4
- 108010070553 Keratin-5 Proteins 0.000 claims 4
- 102000005712 Keratin-8 Human genes 0.000 claims 4
- 108010070511 Keratin-8 Proteins 0.000 claims 4
- 210000003386 epithelial cell of thymus gland Anatomy 0.000 claims 4
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims 4
- 108010049955 Bone Morphogenetic Protein 4 Proteins 0.000 claims 3
- 102100024505 Bone morphogenetic protein 4 Human genes 0.000 claims 3
- 102000004864 Fibroblast growth factor 10 Human genes 0.000 claims 3
- 108090001047 Fibroblast growth factor 10 Proteins 0.000 claims 3
- 102000003972 Fibroblast growth factor 7 Human genes 0.000 claims 3
- 108090000385 Fibroblast growth factor 7 Proteins 0.000 claims 3
- 102000003956 Fibroblast growth factor 8 Human genes 0.000 claims 3
- 108090000368 Fibroblast growth factor 8 Proteins 0.000 claims 3
- 101000599951 Homo sapiens Insulin-like growth factor I Proteins 0.000 claims 3
- 102100037852 Insulin-like growth factor I Human genes 0.000 claims 3
- 238000012258 culturing Methods 0.000 claims 3
- 229940098448 fibroblast growth factor 7 Drugs 0.000 claims 3
- 210000000130 stem cell Anatomy 0.000 claims 3
- QASFUMOKHFSJGL-LAFRSMQTSA-N Cyclopamine Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H](CC2=C3C)[C@@H]1[C@@H]2CC[C@@]13O[C@@H]2C[C@H](C)CN[C@H]2[C@H]1C QASFUMOKHFSJGL-LAFRSMQTSA-N 0.000 claims 2
- 102100021592 Interleukin-7 Human genes 0.000 claims 2
- 108010002586 Interleukin-7 Proteins 0.000 claims 2
- 102000011782 Keratins Human genes 0.000 claims 2
- 108010076876 Keratins Proteins 0.000 claims 2
- 108090001012 Transforming Growth Factor beta Proteins 0.000 claims 2
- 102000004887 Transforming Growth Factor beta Human genes 0.000 claims 2
- 230000024245 cell differentiation Effects 0.000 claims 2
- 239000003795 chemical substances by application Substances 0.000 claims 2
- QASFUMOKHFSJGL-UHFFFAOYSA-N cyclopamine Natural products C1C=C2CC(O)CCC2(C)C(CC2=C3C)C1C2CCC13OC2CC(C)CNC2C1C QASFUMOKHFSJGL-UHFFFAOYSA-N 0.000 claims 2
- 210000004039 endoderm cell Anatomy 0.000 claims 2
- 210000001778 pluripotent stem cell Anatomy 0.000 claims 2
- 208000035473 Communicable disease Diseases 0.000 claims 1
- 102100033553 Delta-like protein 4 Human genes 0.000 claims 1
- 102100023371 Forkhead box protein N1 Human genes 0.000 claims 1
- 101000872077 Homo sapiens Delta-like protein 4 Proteins 0.000 claims 1
- 101000907576 Homo sapiens Forkhead box protein N1 Proteins 0.000 claims 1
- 101001124791 Homo sapiens Proteasome subunit beta type-11 Proteins 0.000 claims 1
- 206010061598 Immunodeficiency Diseases 0.000 claims 1
- 208000029462 Immunodeficiency disease Diseases 0.000 claims 1
- 241000124008 Mammalia Species 0.000 claims 1
- 206010028980 Neoplasm Diseases 0.000 claims 1
- 102000004245 Proteasome Endopeptidase Complex Human genes 0.000 claims 1
- 108090000708 Proteasome Endopeptidase Complex Proteins 0.000 claims 1
- 102100029082 Proteasome subunit beta type-11 Human genes 0.000 claims 1
- 108700022368 Whn Proteins 0.000 claims 1
- -1 Wnt3a Proteins 0.000 claims 1
- 230000001363 autoimmune Effects 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 201000011510 cancer Diseases 0.000 claims 1
- 210000001900 endoderm Anatomy 0.000 claims 1
- 230000007813 immunodeficiency Effects 0.000 claims 1
- 208000015181 infectious disease Diseases 0.000 claims 1
- 239000003112 inhibitor Substances 0.000 claims 1
- 229940100994 interleukin-7 Drugs 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 230000001575 pathological effect Effects 0.000 claims 1
- 230000002265 prevention Effects 0.000 claims 1
- 210000002536 stromal cell Anatomy 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762560908P | 2017-09-20 | 2017-09-20 | |
| US62/560,908 | 2017-09-20 | ||
| PCT/US2018/051625 WO2019060336A1 (en) | 2017-09-20 | 2018-09-19 | IN VITRO GENERATION OF THYMIC ORGANOID FROM HUMAN PLURIPOTENT STEM CELLS |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020534004A JP2020534004A (ja) | 2020-11-26 |
| JP2020534004A5 true JP2020534004A5 (https=) | 2021-11-04 |
| JP7372910B2 JP7372910B2 (ja) | 2023-11-01 |
Family
ID=63794667
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020516436A Active JP7372910B2 (ja) | 2017-09-20 | 2018-09-19 | ヒト多能性幹細胞由来の胸腺オルガノイドのインビトロ生成 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US11898166B2 (https=) |
| EP (1) | EP3684920A1 (https=) |
| JP (1) | JP7372910B2 (https=) |
| WO (1) | WO2019060336A1 (https=) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9719068B2 (en) | 2010-05-06 | 2017-08-01 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into intestinal tissues through directed differentiation |
| SG10201801654RA (en) | 2014-05-28 | 2018-04-27 | Childrens Hospital Med Ct | Methods and systems for converting precursor cells into gastric tissues through directed differentiation |
| EP4553082A3 (en) | 2016-11-04 | 2025-08-20 | Children's Hospital Medical Center | Liver organoid compositions and methods of making and using same |
| JP7248586B2 (ja) | 2017-04-14 | 2023-03-29 | チルドレンズ ホスピタル メディカル センター | 複数ドナー幹細胞組成物およびそれを作製する方法 |
| EP3694603B1 (en) | 2017-10-10 | 2026-04-08 | Children's Hospital Medical Center | Esophageal tissue and/or organoid compositions and methods of making same |
| EP3727394A4 (en) | 2017-12-21 | 2021-09-08 | Children's Hospital Medical Center | DIGITALIZED HUMAN ORGANOIDS AND METHOD OF USING THEREOF |
| EP4678727A3 (en) | 2018-07-26 | 2026-03-11 | Children's Hospital Medical Center | Hepato-biliary-pancreatic tissues and methods of making same |
| CA3112026A1 (en) | 2018-09-12 | 2020-03-19 | Children's Hospital Medical Center | Organoid compositions for the production of hematopoietic stem cells and derivatives thereof |
| US12600943B2 (en) | 2019-02-01 | 2026-04-14 | The University Of Hong Kong | Innervated organoid compositions and methods of making same |
| JP7740706B2 (ja) * | 2019-04-01 | 2025-09-17 | ザ・トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク | 多能性幹細胞の胸腺上皮細胞および胸腺上皮前駆細胞分化を促進する方法 |
| WO2020220040A1 (en) * | 2019-04-26 | 2020-10-29 | The Regents Of The University Of Colorado, A Body Corporate | Generation of functional and patient-specific thymic tissue in vivo from induced pluripotent stem cells |
| US12497597B2 (en) | 2019-05-31 | 2025-12-16 | Children's Hospital Medical Center | Methods of generating and expanding hematopoietic stem cells |
| AU2020283048A1 (en) | 2019-05-31 | 2021-12-23 | Children's Hospital Medical Center | Shaped organoid compositions and methods of making same |
| JP7676361B2 (ja) * | 2019-08-13 | 2025-05-14 | チルドレンズ ホスピタル メディカル センター | オルガノイド組成物を作製するための改良された方法 |
| KR20220113720A (ko) * | 2019-11-25 | 2022-08-16 | 앨리게니 싱어 리서치 인스티튜트 | 인간 전분화능 줄기 세포로부터 생체공학적으로 조작된 흉선 오가노이드 |
| CA3179236A1 (en) * | 2020-04-28 | 2021-11-04 | The Regents Of The University Of California | Methods for generating thymic cells in vitro |
| WO2022076751A1 (en) * | 2020-10-07 | 2022-04-14 | The Regents Of The University Of Colorado, A Body Corporate | Compositions, methods and use for in vitro and in vivo generation of functional thymic tissue |
| GB2601793B (en) * | 2020-12-10 | 2025-07-23 | The Francis Crick Institute Ltd | Thymic constructs and uses thereof |
| WO2023064457A1 (en) * | 2021-10-15 | 2023-04-20 | Thymmune Therapeutics, Inc. | Thymic cells and methods of making |
| US20250332259A1 (en) * | 2022-06-17 | 2025-10-30 | Kyoto University | Thymic epithelial cell production method |
| KR20250109947A (ko) * | 2024-01-11 | 2025-07-18 | 재단법인 아산사회복지재단 | 흉선 세포 또는 흉선 상피세포를 유효성분으로 포함하는 암 예방용 약학적 조성물 |
| CN119614476A (zh) * | 2024-11-13 | 2025-03-14 | 广州市第一人民医院（广州消化疾病中心、广州医科大学附属市一人民医院、华南理工大学附属第二医院） | 胸腺类器官的构建方法和应用 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8034334B2 (en) | 2002-09-06 | 2011-10-11 | The United States Of America As Represented By The Secretary, Department Of Health And Human Services | Immunotherapy with in vitro-selected antigen-specific lymphocytes after non-myeloablative lymphodepleting chemotherapy |
| AU2008247311A1 (en) | 2007-05-03 | 2008-11-13 | Australian Stem Cell Centre Ltd. | Novel thymic cellular populations and uses thereof |
| WO2010143529A1 (ja) * | 2009-06-09 | 2010-12-16 | 国立大学法人名古屋大学 | 多能性幹細胞を胸腺上皮へ分化誘導する方法 |
| US8383099B2 (en) | 2009-08-28 | 2013-02-26 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Adoptive cell therapy with young T cells |
| US20120244133A1 (en) | 2011-03-22 | 2012-09-27 | The United States of America, as represented by the Secretary, Department of Health and | Methods of growing tumor infiltrating lymphocytes in gas-permeable containers |
| WO2014066649A1 (en) * | 2012-10-26 | 2014-05-01 | The Regents Of The University Of California | A strategy for engineering various 3d tissues, organoids and vasculature |
| SG11201803419PA (en) | 2015-10-30 | 2018-05-30 | The Regents Of The Universtiy Of California | Methods of generating t-cells from stem cells and immunotherapeutic methods using the t-cells |
-
2018
- 2018-09-19 US US16/648,008 patent/US11898166B2/en active Active
- 2018-09-19 EP EP18783234.0A patent/EP3684920A1/en active Pending
- 2018-09-19 JP JP2020516436A patent/JP7372910B2/ja active Active
- 2018-09-19 WO PCT/US2018/051625 patent/WO2019060336A1/en not_active Ceased
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