JP7370602B2 - 未分化細胞の分化抵抗性評価法 - Google Patents

未分化細胞の分化抵抗性評価法 Download PDF

Info

Publication number
JP7370602B2
JP7370602B2 JP2020566493A JP2020566493A JP7370602B2 JP 7370602 B2 JP7370602 B2 JP 7370602B2 JP 2020566493 A JP2020566493 A JP 2020566493A JP 2020566493 A JP2020566493 A JP 2020566493A JP 7370602 B2 JP7370602 B2 JP 7370602B2
Authority
JP
Japan
Prior art keywords
cells
undifferentiated
differentiation
resistance
promoter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2020566493A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2020149391A1 (ja
JPWO2020149391A5 (fr
Inventor
英樹 谷口
圭輔 関根
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yokohama City University
Original Assignee
Yokohama City University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yokohama City University filed Critical Yokohama City University
Publication of JPWO2020149391A1 publication Critical patent/JPWO2020149391A1/ja
Publication of JPWO2020149391A5 publication Critical patent/JPWO2020149391A5/ja
Application granted granted Critical
Publication of JP7370602B2 publication Critical patent/JP7370602B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2020566493A 2019-01-17 2020-01-17 未分化細胞の分化抵抗性評価法 Active JP7370602B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019005892 2019-01-17
JP2019005892 2019-01-17
PCT/JP2020/001417 WO2020149391A1 (fr) 2019-01-17 2020-01-17 Procédé d'évaluation de la résistance à la différenciation de cellules indifférenciées

Publications (3)

Publication Number Publication Date
JPWO2020149391A1 JPWO2020149391A1 (ja) 2021-12-09
JPWO2020149391A5 JPWO2020149391A5 (fr) 2022-09-05
JP7370602B2 true JP7370602B2 (ja) 2023-10-30

Family

ID=71614449

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2020566493A Active JP7370602B2 (ja) 2019-01-17 2020-01-17 未分化細胞の分化抵抗性評価法

Country Status (2)

Country Link
JP (1) JP7370602B2 (fr)
WO (1) WO2020149391A1 (fr)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014523735A (ja) 2011-07-25 2014-09-18 国立大学法人京都大学 人工多能性幹細胞の選別方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017184623A (ja) * 2016-04-01 2017-10-12 公益財団法人ヒューマンサイエンス振興財団 未分化細胞間における分化傾向の評価方法、分化傾向の評価マーカーとしてのＳＡＬＬ３ ｍＲＮＡ、及び、未分化細胞の分化能力の制御方法
JP7173496B2 (ja) * 2017-10-20 2022-11-16 国立大学法人大阪大学 心筋細胞への分化指向性を有する多能性幹細胞を選抜するための方法
CN112262217A (zh) * 2018-06-15 2021-01-22 公立大学法人横滨市立大学 未分化细胞检测法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014523735A (ja) 2011-07-25 2014-09-18 国立大学法人京都大学 人工多能性幹細胞の選別方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
International Journal of Stem Cells，2011年，Vol.4, No.1，PP.35-42
Nature，2017年，Vol.546，PP.533-538，https://doi.org/10.1038/nature22796
佐藤陽治 et al.，分化プロペンシティを指標とした細胞特性解析法の開発，厚生労働科学研究費補助金 医薬品・医療機器等レギュラトリーサイエンス総合研究事業 細胞・組織加工製品，2014年06月16日，平成２５年度 総括・分担研究報告書，pp. 117-143

Also Published As

Publication number Publication date
JPWO2020149391A1 (ja) 2021-12-09
WO2020149391A1 (fr) 2020-07-23

Similar Documents

Publication Publication Date Title
US20210371914A1 (en) Methods and compositions for identifying or quantifying targets in a biological sample
Adams A beginner’s guide to RT-PCR, qPCR and RT-qPCR
JP2023040233A (ja) 分子の多重検出方法
US7049103B2 (en) Method of evaluating drug efficacy and toxicity
US20230193246A1 (en) Methods and compositions for detecting targets
US7906288B2 (en) Compare-MS: method rapid, sensitive and accurate detection of DNA methylation
EP2660331B1 (fr) Procédé pour l'analyse de génome de cellule unique et trousse correspondante
JP7041934B2 (ja) 未分化細胞検出法
Van Doorn et al. Quantitative multiplex detection of plant pathogens using a novel ligation probe-based system coupled with universal, high-throughput real-time PCR on OpenArrays™
JP7344565B2 (ja) 未分化細胞検出法
KR101317263B1 (ko) 리얼타임 ｐｃｒ을 이용한 카바페넴 내성 장내균 검사 방법 및 키트
JP7370602B2 (ja) 未分化細胞の分化抵抗性評価法
KR20160056841A (ko) Ｆｌｔ３ 유전자 변이 정량분석방법 및 분석 키트
JPWO2021095798A1 (ja) 未分化マーカー遺伝子高感度検出法
KR101683086B1 (ko) 유전자의 발현량 및 메틸화 프로필을 활용한 돼지의 산자수 예측방법
JP2017175953A (ja) Ｓｙｔ−ｓｓｘ融合遺伝子検出用プローブ、ｓｙｔ−ｓｓｘ融合遺伝子検出用プローブセット、ｓｙｔ−ｓｓｘ融合遺伝子の検出方法及びｓｙｔ−ｓｓｘ融合遺伝子検出用キット
GB2589869A (en) Method for whole genome sequencing of picogram quantities of DNA
van Buggenum et al. Immuno-Detection by sequencing (ID-seq) enables large-scale high-dimensional phenotyping in cells
US20170081713A1 (en) Multivalent probes having single nucleotide resolution
WO2021010446A1 (fr) Procédé pour la détection d'une bactérie mutante résistante aux antibiotiques macrolides
Powell et al. Tissue source attribution using the PyroMark® Q48 Autoprep System: Sperm identification in forensic casework
Morowitz Modulation of Epigenetic Mark 5-hydroxymethylcytosine in Intestinal Organoid Cultures
Xu et al. A Developed Accurate Digital PCR Detection Technology in Food Safety
Cankovic Laboratory Testing for Prognostic and Predictive Markers in Gliomas
Sykes et al. dPCR-digital Polymerase Chain Reaction (3)

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220825

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20220825

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20230726

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230830

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20231004

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20231011

R150 Certificate of patent or registration of utility model

Ref document number: 7370602

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150