JP7261538B2 - 乳房上皮幹細胞を増殖させるための培養培地 - Google Patents

乳房上皮幹細胞を増殖させるための培養培地 Download PDF

Info

Publication number
JP7261538B2
JP7261538B2 JP2017528101A JP2017528101A JP7261538B2 JP 7261538 B2 JP7261538 B2 JP 7261538B2 JP 2017528101 A JP2017528101 A JP 2017528101A JP 2017528101 A JP2017528101 A JP 2017528101A JP 7261538 B2 JP7261538 B2 JP 7261538B2
Authority
JP
Japan
Prior art keywords
culture medium
cells
organoids
cell
breast
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017528101A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017536827A (ja
Inventor
ラース ノーマン ザックス
アナ グラカニン
ヤルノ ドロスト
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koninklijke Nederlandse Akademie van Wetenschappen
Original Assignee
Koninklijke Nederlandse Akademie van Wetenschappen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninklijke Nederlandse Akademie van Wetenschappen filed Critical Koninklijke Nederlandse Akademie van Wetenschappen
Publication of JP2017536827A publication Critical patent/JP2017536827A/ja
Priority to JP2021179965A priority Critical patent/JP7602446B2/ja
Application granted granted Critical
Publication of JP7261538B2 publication Critical patent/JP7261538B2/ja
Priority to JP2024072368A priority patent/JP2024102169A/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0693Tumour cells; Cancer cells
    • C12N5/0695Stem cells; Progenitor cells; Precursor cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0625Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0631Mammary cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/115Basic fibroblast growth factor (bFGF, FGF-2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40Regulators of development
    • C12N2501/415Wnt; Frizzeled
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Dermatology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2017528101A 2014-11-27 2015-11-27 乳房上皮幹細胞を増殖させるための培養培地 Active JP7261538B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2021179965A JP7602446B2 (ja) 2014-11-27 2021-11-04 乳房上皮幹細胞を増殖させるための培養培地
JP2024072368A JP2024102169A (ja) 2014-11-27 2024-04-26 乳房上皮幹細胞を増殖させるための培養培地

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1421094.2A GB201421094D0 (en) 2014-11-27 2014-11-27 Culture medium
GB1421094.2 2014-11-27
PCT/EP2015/077988 WO2016083612A1 (en) 2014-11-27 2015-11-27 Culture medium for expanding breast epithelial stem cells

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2021179965A Division JP7602446B2 (ja) 2014-11-27 2021-11-04 乳房上皮幹細胞を増殖させるための培養培地

Publications (2)

Publication Number Publication Date
JP2017536827A JP2017536827A (ja) 2017-12-14
JP7261538B2 true JP7261538B2 (ja) 2023-04-20

Family

ID=52349562

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2017528101A Active JP7261538B2 (ja) 2014-11-27 2015-11-27 乳房上皮幹細胞を増殖させるための培養培地
JP2021179965A Active JP7602446B2 (ja) 2014-11-27 2021-11-04 乳房上皮幹細胞を増殖させるための培養培地
JP2024072368A Pending JP2024102169A (ja) 2014-11-27 2024-04-26 乳房上皮幹細胞を増殖させるための培養培地

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2021179965A Active JP7602446B2 (ja) 2014-11-27 2021-11-04 乳房上皮幹細胞を増殖させるための培養培地
JP2024072368A Pending JP2024102169A (ja) 2014-11-27 2024-04-26 乳房上皮幹細胞を増殖させるための培養培地

Country Status (9)

Country Link
US (3) US10961511B2 (enExample)
EP (2) EP3224351B1 (enExample)
JP (3) JP7261538B2 (enExample)
AU (1) AU2015352350B2 (enExample)
DK (1) DK3224351T3 (enExample)
ES (1) ES2883159T3 (enExample)
GB (1) GB201421094D0 (enExample)
PT (1) PT3224351T (enExample)
WO (1) WO2016083612A1 (enExample)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2412800A1 (en) 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Liver organoid, uses thereof and culture method for obtaining them
EP3061808B1 (en) 2009-02-03 2020-08-12 Koninklijke Nederlandse Akademie van Wetenschappen Culture medium for epithelial stem cells and organoids comprising said stem cells
WO2011140441A2 (en) 2010-05-06 2011-11-10 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
TR201815226T4 (tr) 2011-12-19 2018-11-21 Koninklijke Nederlandse Akademie Van Wetenschappen Birincil intestinal kültür modelindeki cftr fonksiyonunu ölçmek için hızlı ve nicel bir analiz.
US10597633B2 (en) 2014-05-16 2020-03-24 Koninklijke Nederlandse Akademie Van Wetenschappen Culture method for organoids
EP3149156B1 (en) 2014-05-28 2021-02-17 Children's Hospital Medical Center Methods and systems for converting precursor cells into gastric tissues through directed differentiation
US11584916B2 (en) 2014-10-17 2023-02-21 Children's Hospital Medical Center Method of making in vivo human small intestine organoids from pluripotent stem cells
GB201421092D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
GB201421094D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
GB201603569D0 (en) 2016-03-01 2016-04-13 Koninklijke Nederlandse Akademie Van Wetenschappen Improved differentiation method
CN116790476A (zh) 2016-05-05 2023-09-22 儿童医院医疗中心 用于体外制造胃底组织的方法和与其相关的组合物
GB201611982D0 (en) 2016-07-11 2016-08-24 Cellesce Ltd Cell culture
EP3523418A4 (en) * 2016-10-06 2020-06-17 Transgenex Nanobiotech, Inc. METHOD FOR THE EXPANSION OF CANCER STEM CELLS (CSC)
KR102546194B1 (ko) 2016-11-04 2023-06-21 칠드런즈 호스피탈 메디칼 센터 간 유사 장기 조성물 및 이를 제조 및 사용하는 방법
KR102558606B1 (ko) 2016-12-05 2023-07-26 칠드런즈 호스피탈 메디칼 센터 결장 유사장기 및 이를 제조 및 사용하는 방법
WO2018191673A1 (en) 2017-04-14 2018-10-18 Children's Hospital Medical Center Multi donor stem cell compositions and methods of making same
EP3630949A4 (en) 2017-05-29 2021-03-10 Stemcell Technologies Canada Inc. COMPOSITIONS AND PROCESSES FOR OBTAINING ORGANOIDS
WO2019006111A1 (en) * 2017-06-28 2019-01-03 Rutgers, The State University Of New Jersey ORGANOIDS DERIVED FROM A SINGLE MAMMARY CELL
EP3694603A4 (en) 2017-10-10 2021-07-14 Children's Hospital Medical Center ESOPHAGUS TISSUE AND / OR ORGANOID COMPOSITIONS AND METHOD FOR MANUFACTURING THEREOF
EP3727394A4 (en) 2017-12-21 2021-09-08 Children's Hospital Medical Center DIGITALIZED HUMAN ORGANOIDS AND METHOD OF USING THEREOF
TWI703215B (zh) * 2017-12-28 2020-09-01 財團法人生物技術開發中心 用於預測藥物功效之活體外診斷方法
AU2019242912B2 (en) 2018-03-28 2025-07-17 Board Of Regents, The University Of Texas System Identification of epigenetic alterations in DNA isolated from exosomes
WO2019199881A1 (en) * 2018-04-09 2019-10-17 Cedars-Sinai Medical Center Methods for in vitro expansion of adult tissue stem cells
JP7506657B2 (ja) 2018-07-26 2024-06-26 チルドレンズ ホスピタル メディカル センター 肝胆膵組織およびその作製方法
US12428622B2 (en) 2018-09-12 2025-09-30 Children's Hospital Medical Center Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
BR112021006067A2 (pt) * 2018-09-27 2021-07-20 The Regents Of The University Of California métodos de isolação e cultura de célula progenitora retinal humana
CA3116711A1 (en) * 2018-10-16 2020-04-23 Board Of Regents,The University Of Texas System Compositions for and methods of producing tumor organoids
GB201819224D0 (en) 2018-11-26 2019-01-09 Koninklijke Nederlandse Akademie Van Wetenschappen Hepatocyte expansion methods
GB201903573D0 (en) 2019-03-15 2019-05-01 Koninklijke Nederlandse Akademie Van Wetenschappen Culture method for head and neck organoids
GB201906978D0 (en) 2019-05-17 2019-07-03 Koninklijke Nederlandse Akademie Van Wetenschappen Improved culture method using integrin agonist
JP7610533B2 (ja) 2019-05-31 2025-01-08 チルドレンズ ホスピタル メディカル センター 造血幹細胞の生成および拡大方法
BR112022005225A2 (pt) * 2019-09-19 2022-08-16 Univ Northwestern Meio de cultura econômico e protocolo para células-tronco pluripotentes induzidas humanas
JP2022551869A (ja) * 2019-10-10 2022-12-14 ユニバーシティー オブ ヒューストン システム 重層上皮幹細胞のためのフィーダーベースおよびフィーダーフリーの幹細胞培養系ならびにそれらに関連した使用方法
WO2021081253A1 (en) 2019-10-22 2021-04-29 Tempus Labs, Inc. Systems and methods for predicting therapeutic sensitivity
CN112779209B (zh) * 2019-11-08 2023-01-24 合肥中科普瑞昇生物医药科技有限公司 原代乳腺上皮细胞培养基、培养方法及其应用
US11629385B2 (en) 2019-11-22 2023-04-18 Tempus Labs, Inc. Tumor organoid culture compositions, systems, and methods
EP4070098B1 (en) 2019-12-05 2025-12-24 Tempus AI, Inc. Large scale organoid analysis
CN111424015A (zh) * 2019-12-10 2020-07-17 重庆康克唯生物科技有限公司 用于前列腺肿瘤细胞的培养基和三维培养方法
CN113528425B (zh) * 2020-04-15 2023-05-30 合肥中科普瑞昇生物医药科技有限公司 一种用于乳腺上皮肿瘤细胞的培养基和培养方法
US11561178B2 (en) 2020-04-20 2023-01-24 Tempus Labs, Inc. Artificial fluorescent image systems and methods
NL2027546B1 (en) * 2021-02-11 2022-09-12 Prinses Maxima Centrum Voor Kinderoncologie B V Method of producing an organoid
CA3215516A1 (en) * 2021-04-16 2022-10-20 David ROWBOTHAM Media and methods for growing mammary organoids
TWI794821B (zh) * 2021-05-20 2023-03-01 國立陽明交通大學 用於在體外培養原代人類肺泡上皮細胞的方法
CN117821380A (zh) * 2021-07-05 2024-04-05 浙江大学 Fgf7在制备促进干细胞体外扩增和维持表型的试剂中的应用
CN114317442B (zh) * 2022-01-07 2025-02-07 重庆嘉士腾医药有限公司 一种建立乳腺类器官或/和乳腺癌类器官的培养基及方法和应用
WO2023137465A1 (en) * 2022-01-13 2023-07-20 BIOMILQ, Inc. Optimization of live cell constructs for production of cultured milk product and methods of using the same
CN114591887B (zh) * 2022-03-14 2022-10-14 杭州艾名医学科技有限公司 一种乳腺癌类器官培养液及制备方法和培养方法
GB202204266D0 (en) 2022-03-25 2022-05-11 Stichting Hubrecht Organoid Tech Methods for co-culturing genotoxic bacteria and organoids
EP4495227A1 (en) 2023-07-18 2025-01-22 HUB Organoids IP B.V. Co-cultures of organoids and stromal cells

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012525148A (ja) 2009-04-27 2012-10-22 バイアサイト インク 低分子サポート多能性細胞成育およびその方法
WO2014066649A1 (en) 2012-10-26 2014-05-01 The Regents Of The University Of California A strategy for engineering various 3d tissues, organoids and vasculature
JP2014516562A (ja) 2011-06-10 2014-07-17 コーニンクレッカ ネザーランド アカデミー ヴァン ウェテンシャッペン 幹細胞のための培養培地

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09512910A (ja) 1994-05-04 1997-12-22 マウント・サイナイ・ホスピタル・コーポレイション TGF−βスーパーファミリーのサイトカインの変調因子およびそのアッセイ方法
EP0953633A1 (en) 1998-04-28 1999-11-03 Livercell L.L.C. Cell culturing method and medium for producing proliferated, normal, differentiated human liver cells
WO2000050049A2 (en) 1999-02-24 2000-08-31 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Artificial salivary gland
US20030003088A1 (en) 2001-05-03 2003-01-02 Board Of Trustees Operating Michigan State University Human pancreatic pluripotential stem cell line
AU2002367580A1 (en) 2001-05-16 2003-09-22 Tracy C. Grikscheit Tissue-engineered organs
AU2002331669A1 (en) 2001-08-23 2003-03-10 The Wistar Institute Of Anatomy And Biology An organotypic intestinal culture and methods of use thereof
CA2463914A1 (en) 2001-10-18 2003-04-24 Ixion Biotechnology, Inc. Conversion of liver stem and progenitor cells to pancreatic functional cells
WO2004050827A2 (en) 2002-12-05 2004-06-17 Technion Research & Development Foundation Ltd. Cultured human pancreatic islets, and uses thereof
WO2004087896A2 (en) 2003-03-31 2004-10-14 Pfizer Products Inc. Hepatocyte differentiation of stem cells
US20040229355A1 (en) 2003-05-14 2004-11-18 Board Of Regents University Of Texas System Culture medium for long-term culture of hepatocytes
US20070010008A1 (en) 2005-06-29 2007-01-11 Tissuetech, Inc. Ex vivo expansion of primary animal cells
WO2005040391A1 (en) 2003-10-27 2005-05-06 Murdoch Childrens Research Institute Compositions and methods for differentiating stem cells
US20070036769A9 (en) 2004-06-03 2007-02-15 Linheng Li BMP pathway methods and compositions
GB0413005D0 (en) 2004-06-11 2004-07-14 Coletica Ligand
US8492148B2 (en) 2005-02-23 2013-07-23 Foundation For Biomedical Research & Innovation Method for amplification of endothelial progenitor cell in vitro
AU2006202209B2 (en) 2005-05-27 2011-04-14 Lifescan, Inc. Amniotic fluid derived cells
WO2007030693A2 (en) 2005-09-08 2007-03-15 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Methods for promoting stem cell proliferation and survival
DK2420565T3 (en) 2006-02-23 2017-12-04 Viacyte Inc APPLICABLE COMPOSITIONS AND METHODS FOR CULTIVATING DIFFERENTIBLE CELLS
US7989204B2 (en) 2006-04-28 2011-08-02 Viacyte, Inc. Hepatocyte lineage cells
US8628964B2 (en) 2006-10-11 2014-01-14 Drexel University Fetal pulmonary cells and uses thereof
CA2664828A1 (en) 2006-10-20 2008-04-24 Deutsches Krebsforschungszentrum Stiftung Des Offentlichen Rechts Rspondins as modulators of angiogenesis and vasculogenesis
US9251637B2 (en) 2006-11-15 2016-02-02 Bank Of America Corporation Method and apparatus for using at least a portion of a one-time password as a dynamic card verification value
US20080182328A1 (en) 2006-12-19 2008-07-31 The Burnham Institute Mammalian extraembryonic endoderm cells and methods of isolation
US20080233088A1 (en) 2007-02-16 2008-09-25 Varian Medical Systems Technologies, Inc. Preparative regimen for engraftment, growth and differentiation of non-hematopoeitic cells in vivo after transplantation
EP2022848A1 (en) 2007-08-10 2009-02-11 Hubrecht Institut A method for identifying, expanding, and removing adult stem cells and cancer stem cells
JP2009195142A (ja) 2008-02-20 2009-09-03 Institute Of Physical & Chemical Research 肝臓卵形細胞の取得方法
CA2723648A1 (en) * 2008-04-10 2009-10-15 Piyush Gupta Methods for identification and use of agents targeting cancer stem cells
WO2010011352A2 (en) * 2008-07-25 2010-01-28 The University Of Georgia Research Foundation, Inc. Compositions for mesoderm derived isl1+ multipotent cells (imps), epicardial progenitor cells (epcs) and multipotent cxcr4+cd56+ cells (c56cs) and methods of use
WO2010016766A2 (en) 2008-08-08 2010-02-11 Koninklijke Nederlandse Akademie Van Wetenschappen Antibodies recognizing endogenous human lgr5 and/or lgr6
US9464275B2 (en) 2008-08-21 2016-10-11 The Board Of Trustees Of The Leland Stanford Junior University Ex vivo culture, proliferation and expansion of intestinal epithelium
EP2412800A1 (en) 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Liver organoid, uses thereof and culture method for obtaining them
US9752124B2 (en) 2009-02-03 2017-09-05 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for epithelial stem cells and organoids comprising the stem cells
EP3061808B1 (en) 2009-02-03 2020-08-12 Koninklijke Nederlandse Akademie van Wetenschappen Culture medium for epithelial stem cells and organoids comprising said stem cells
WO2010108126A2 (en) 2009-03-19 2010-09-23 Fate Therapeutics, Inc. Reprogramming compositions and methods of using the same
EP2580320B1 (en) 2010-06-14 2018-08-01 The Scripps Research Institute Reprogramming of cells to a new fate
SG188666A1 (en) 2010-09-30 2013-05-31 Agency Science Tech & Res Methods and reagents for detection and treatment of esophageal metaplasia
KR102482184B1 (ko) 2010-12-22 2022-12-28 페이트 세러퓨틱스, 인코포레이티드 단세포 분류 및 iPSC의 증강된 재프로그래밍을 위한 세포 배양 플랫폼
GB201106395D0 (en) 2011-04-14 2011-06-01 Hubrecht Inst Compounds
CA2839102A1 (en) 2011-06-29 2013-01-03 The Penn State Research Foundation Compositions, methods and kits for treating leukemia
US20130052729A1 (en) 2011-08-29 2013-02-28 Olivier Pourquie Method for preparing induced paraxial mesoderm progenitor (ipam) cells and their use
US9663573B2 (en) 2011-10-05 2017-05-30 Genentech, Inc. Methods of treating liver conditions using Notch2 antagonists
GB201117469D0 (en) 2011-10-10 2011-11-23 Cell Guidance Systems Ltd Culture media for pluripotent stem cells
JP6238445B2 (ja) 2011-10-27 2017-11-29 国立大学法人 東京医科歯科大学 大腸上皮幹細胞の単離・培養技術と、これを用いた大腸上皮移植技術
US20140302491A1 (en) 2011-10-28 2014-10-09 The Board Of Trustees Of The Leland Stanford Junior University Ex Vivo Culture, Proliferation and Expansion of Primary Tissue Organoids
TR201815226T4 (tr) 2011-12-19 2018-11-21 Koninklijke Nederlandse Akademie Van Wetenschappen Birincil intestinal kültür modelindeki cftr fonksiyonunu ölçmek için hızlı ve nicel bir analiz.
WO2014127170A1 (en) 2013-02-13 2014-08-21 Wake Forest University Health Sciences Bioengineered liver constructs and methods relating thereto
WO2014127219A1 (en) 2013-02-14 2014-08-21 The Cleveland Clinic Foundation Methods for induction of cell fates from pluripotent cells
AU2014218290B2 (en) 2013-02-18 2020-03-12 The Hospital For Sick Children Methods for generating hepatocytes and cholangiocytes from pluripotent stem cells
CA2905842C (en) 2013-03-14 2023-02-21 The Brigham And Women's Hospital, Inc. Compositions and methods for epithelial stem cell expansion comprising a wnt agonist and a histone deacetylase inhibitor
PT3929207T (pt) 2013-03-15 2024-10-21 Univ Texas Utilização do péptido fttftvt no tratamento de fibrose
CA3205855A1 (en) 2013-04-16 2014-10-23 Orbsen Therapeutics Limited Medical use of syndecan-2
US10597633B2 (en) 2014-05-16 2020-03-24 Koninklijke Nederlandse Akademie Van Wetenschappen Culture method for organoids
US11920164B2 (en) 2014-07-30 2024-03-05 Yeda Research And Development Co. Ltd. Media for culturing naive human pluripotent stem cells
EP3204489A4 (en) 2014-10-08 2018-09-05 Agency for Science, Technology and Research Methods of differentiating stem cells into liver cell lineages
GB201421094D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
GB201421092D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
GB201603569D0 (en) 2016-03-01 2016-04-13 Koninklijke Nederlandse Akademie Van Wetenschappen Improved differentiation method
GB201610748D0 (en) 2016-06-20 2016-08-03 Koninklijke Nederlandse Akademie Van Wetenschappen Improved diffrentation method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012525148A (ja) 2009-04-27 2012-10-22 バイアサイト インク 低分子サポート多能性細胞成育およびその方法
JP2014516562A (ja) 2011-06-10 2014-07-17 コーニンクレッカ ネザーランド アカデミー ヴァン ウェテンシャッペン 幹細胞のための培養培地
WO2014066649A1 (en) 2012-10-26 2014-05-01 The Regents Of The University Of California A strategy for engineering various 3d tissues, organoids and vasculature

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Pasic et al.,Sustained activation of the HER1-ERK1/2-RSK signaling pathway controls myoepithelial cell fate in human mammary tissue,GENES & DEVELOPMENT,2011年,Vol.25, p.1641-1653

Also Published As

Publication number Publication date
ES2883159T3 (es) 2021-12-07
EP3224351B1 (en) 2021-06-30
PT3224351T (pt) 2021-09-03
US20170342385A1 (en) 2017-11-30
NZ732345A (en) 2024-12-20
EP3224351A1 (en) 2017-10-04
EP3950937A1 (en) 2022-02-09
JP2017536827A (ja) 2017-12-14
JP2022025124A (ja) 2022-02-09
JP2024102169A (ja) 2024-07-30
AU2015352350B2 (en) 2021-07-29
HK1245324A1 (zh) 2018-08-24
US20250154472A1 (en) 2025-05-15
JP7602446B2 (ja) 2024-12-18
US20210254017A1 (en) 2021-08-19
CA2968751A1 (en) 2016-06-02
US10961511B2 (en) 2021-03-30
AU2015352350A1 (en) 2017-06-15
DK3224351T3 (da) 2021-08-23
GB201421094D0 (en) 2015-01-14
WO2016083612A1 (en) 2016-06-02

Similar Documents

Publication Publication Date Title
JP7602446B2 (ja) 乳房上皮幹細胞を増殖させるための培養培地
JP7319027B2 (ja) 培養培地
US12331314B2 (en) Culture method for organoids
AU2020200515B2 (en) Culture media for stem cells
HK40069127A (en) Culture medium for expanding breast epithelial stem cells
CA2968751C (en) Culture medium for expanding breast epithelial stem cells
HK1245324B (en) Culture medium for expanding breast epithelial stem cells

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180117

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20181126

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20190925

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20191007

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20191227

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20200706

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20201001

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210104

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20210127

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20210705

C60 Trial request (containing other claim documents, opposition documents)

Free format text: JAPANESE INTERMEDIATE CODE: C60

Effective date: 20211104

C116 Written invitation by the chief administrative judge to file amendments

Free format text: JAPANESE INTERMEDIATE CODE: C116

Effective date: 20211117

C22 Notice of designation (change) of administrative judge

Free format text: JAPANESE INTERMEDIATE CODE: C22

Effective date: 20211117

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20211213

C22 Notice of designation (change) of administrative judge

Free format text: JAPANESE INTERMEDIATE CODE: C22

Effective date: 20221031

C22 Notice of designation (change) of administrative judge

Free format text: JAPANESE INTERMEDIATE CODE: C22

Effective date: 20221124

C13 Notice of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: C13

Effective date: 20221212

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230131

C23 Notice of termination of proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C23

Effective date: 20230213

C03 Trial/appeal decision taken

Free format text: JAPANESE INTERMEDIATE CODE: C03

Effective date: 20230313

C30A Notification sent

Free format text: JAPANESE INTERMEDIATE CODE: C3012

Effective date: 20230313

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20230410

R150 Certificate of patent or registration of utility model

Ref document number: 7261538

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150