JP7197134B2 - 蛍光光度計および観測方法 - Google Patents
蛍光光度計および観測方法 Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
- G01J3/0218—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using optical fibers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
- G01J3/4406—Fluorescence spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6423—Spectral mapping, video display
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6484—Optical fibres
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/069—Supply of sources
- G01N2201/0694—Microprocessor controlled supply
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/08—Optical fibres; light guides
- G01N2201/0833—Fibre array at detector, resolving
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- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Description
10 光度計部
11 光源
12 励起側分光器
15 蛍光側分光器
16 検知器
17 試料室
50 試料容器
60 光ファイバーユニット(導光部材)
61 励起側ファイバー(励起光導光部材)
62 蛍光側ファイバー(蛍光導光部材)
63 イメージファイバー(撮影部)
S 試料
Claims (6)
- 光源と、当該光源の光を分光して励起光を生成する励起側分光器と、前記励起光が照射された試料から放出された蛍光を単色光に分光する蛍光側分光器と、を少なくとも備える光度計部と、
前記光度計部の外部に存在する前記試料まで前記励起光を導くとともに、前記試料から放出された前記蛍光を前記光度計部まで導く導光部材と、
を備える蛍光光度計であって、
前記導光部材が、
前記試料を撮影する撮影部と、
前記撮影部の周囲に配置され、前記励起光を導いて前記試料に照射する励起光導光部材と、
前記撮影部の周囲に配置され、前記試料から放出された前記蛍光を前記光度計部まで導く蛍光導光部材と、を備え、
前記励起光導光部材および前記蛍光導光部材が、前記撮影部を取り囲むように存在する、
蛍光光度計。 - 請求項1に記載の蛍光光度計であって、
前記撮影部は、前記試料に照射された前記励起光の反射光によって前記試料を撮影する、
蛍光光度計。 - 請求項1または2に記載の蛍光光度計であって、
前記撮影部が、複数の光ファイバーを束ねて構成されるイメージファイバーであり、
前記励起光導光部材および前記蛍光導光部材の各々が、複数の光ファイバーから構成され、当該複数の光ファイバーが、前記イメージファイバーの周囲においてリング状に配置されている、
蛍光光度計。 - 請求項1から3のいずれか1項に記載の蛍光光度計であって、
前記導光部材は、前記光度計部の筐体から突出した線状の光ファイバーユニットである、
蛍光光度計。 - 試料まで励起光を導くとともに、前記試料から放出された蛍光を導く導光部材を有する蛍光光度計を用いて、前記試料を観測する観測方法であって、
前記導光部材の撮影部によって前記試料を撮影して画像を取得し、
前記撮影部の周囲に配置された励起光導光部材によって前記励起光を前記試料に照射するともに、前記撮影部の周囲に配置された蛍光導光部材によって、前記試料から放出された前記蛍光に基づく蛍光スペクトルを取得し、
前記励起光の波長を連続的に変化させつつ、前記画像の取得と前記蛍光スペクトルの取得を同時に行う、
観測方法。 - 請求項5に記載の観測方法であって、
連続的に変化させた前記励起光の励起波長と、前記蛍光の蛍光波長と、前記蛍光スペクトルの蛍光強度の三事象にて三次元蛍光スペクトルを取得する、
観測方法。
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JP2019044976A JP7197134B2 (ja) | 2019-03-12 | 2019-03-12 | 蛍光光度計および観測方法 |
US16/813,538 US11486828B2 (en) | 2019-03-12 | 2020-03-09 | Fluorescence photometer and observation method |
EP20161997.0A EP3709004B1 (en) | 2019-03-12 | 2020-03-10 | Fluorescence photometer and observation method |
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JP2019044976A JP7197134B2 (ja) | 2019-03-12 | 2019-03-12 | 蛍光光度計および観測方法 |
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EP (1) | EP3709004B1 (ja) |
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KR102476186B1 (ko) * | 2018-12-06 | 2022-12-12 | 주식회사 히타치하이테크 | 하전 입자선 장치 |
JP2022102776A (ja) * | 2020-12-25 | 2022-07-07 | 株式会社日立ハイテクサイエンス | 分光蛍光光度計、分光蛍光測定方法及び画像撮像方法 |
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EP3709004B1 (en) | 2022-10-19 |
JP2020148547A (ja) | 2020-09-17 |
EP3709004A1 (en) | 2020-09-16 |
US11486828B2 (en) | 2022-11-01 |
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