JP7148552B2 - 肝臓オルガノイド組成物ならびにその作製および使用方法 - Google Patents

肝臓オルガノイド組成物ならびにその作製および使用方法 Download PDF

Info

Publication number
JP7148552B2
JP7148552B2 JP2019564915A JP2019564915A JP7148552B2 JP 7148552 B2 JP7148552 B2 JP 7148552B2 JP 2019564915 A JP2019564915 A JP 2019564915A JP 2019564915 A JP2019564915 A JP 2019564915A JP 7148552 B2 JP7148552 B2 JP 7148552B2
Authority
JP
Japan
Prior art keywords
liver
organoids
cells
organoid
drug
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2019564915A
Other languages
English (en)
Japanese (ja)
Other versions
JP2020523000A (ja
JP2020523000A5 (cg-RX-API-DMAC7.html
Inventor
貴則 武部
忠紘 篠澤
小池博之
昌樹 木村
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Science and Technology Agency
Cincinnati Children's Hospital Medical Center
Original Assignee
Japan Science and Technology Agency
Cincinnati Children's Hospital Medical Center
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Science and Technology Agency, Cincinnati Children's Hospital Medical Center filed Critical Japan Science and Technology Agency
Publication of JP2020523000A publication Critical patent/JP2020523000A/ja
Publication of JP2020523000A5 publication Critical patent/JP2020523000A5/ja
Priority to JP2022150773A priority Critical patent/JP7749290B2/ja
Application granted granted Critical
Publication of JP7148552B2 publication Critical patent/JP7148552B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/407—Liver; Hepatocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/237—Oncostatin M [OSM]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Food Science & Technology (AREA)
  • Physiology (AREA)
  • Nutrition Science (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
JP2019564915A 2017-06-09 2018-02-19 肝臓オルガノイド組成物ならびにその作製および使用方法 Expired - Fee Related JP7148552B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022150773A JP7749290B2 (ja) 2017-06-09 2022-09-22 肝臓オルガノイド組成物ならびにその作製および使用方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762517414P 2017-06-09 2017-06-09
US62/517,414 2017-06-09
PCT/US2018/018585 WO2018226267A1 (en) 2017-06-09 2018-02-19 Liver organoid compositions and methods of making and using same

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2022150773A Division JP7749290B2 (ja) 2017-06-09 2022-09-22 肝臓オルガノイド組成物ならびにその作製および使用方法

Publications (3)

Publication Number Publication Date
JP2020523000A JP2020523000A (ja) 2020-08-06
JP2020523000A5 JP2020523000A5 (cg-RX-API-DMAC7.html) 2021-04-01
JP7148552B2 true JP7148552B2 (ja) 2022-10-05

Family

ID=64566329

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2019564915A Expired - Fee Related JP7148552B2 (ja) 2017-06-09 2018-02-19 肝臓オルガノイド組成物ならびにその作製および使用方法
JP2022150773A Active JP7749290B2 (ja) 2017-06-09 2022-09-22 肝臓オルガノイド組成物ならびにその作製および使用方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2022150773A Active JP7749290B2 (ja) 2017-06-09 2022-09-22 肝臓オルガノイド組成物ならびにその作製および使用方法

Country Status (10)

Country Link
US (1) US20200199537A1 (cg-RX-API-DMAC7.html)
EP (1) EP3635095A4 (cg-RX-API-DMAC7.html)
JP (2) JP7148552B2 (cg-RX-API-DMAC7.html)
KR (2) KR102625361B1 (cg-RX-API-DMAC7.html)
CN (1) CN110914408A (cg-RX-API-DMAC7.html)
AU (2) AU2018279790B2 (cg-RX-API-DMAC7.html)
CA (1) CA3065759A1 (cg-RX-API-DMAC7.html)
IL (1) IL270714B2 (cg-RX-API-DMAC7.html)
NZ (1) NZ759164A (cg-RX-API-DMAC7.html)
WO (1) WO2018226267A1 (cg-RX-API-DMAC7.html)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2022119862A (ja) * 2016-11-04 2022-08-17 チルドレンズ ホスピタル メディカル センター 肝臓オルガノイド組成物ならびにその作製および使用方法
JP2022191263A (ja) * 2017-06-09 2022-12-27 チルドレンズ ホスピタル メディカル センター 肝臓オルガノイド組成物ならびにその作製および使用方法
US12241090B2 (en) 2014-05-28 2025-03-04 Children's Hospital Medical Center Methods and systems for converting precursor cells into gastric tissues through directed differentiation
US12258584B2 (en) 2010-05-06 2025-03-25 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
US12281334B2 (en) 2017-04-14 2025-04-22 Children's Hospital Medical Center Multi donor stem cell compositions and methods of making same
US12297457B2 (en) 2017-10-10 2025-05-13 Children's Hospital Medical Center Esophageal tissue and/or organoid compositions and methods of making same
US12379372B2 (en) 2017-12-21 2025-08-05 Children's Hospital Medical Center Digitalized human organoids and methods of using same
US12421500B2 (en) 2018-07-26 2025-09-23 Children's Hospital Medical Center Hepato-biliary-pancreatic tissues and methods of making same
US12428622B2 (en) 2018-09-12 2025-09-30 Children's Hospital Medical Center Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
US12497597B2 (en) 2019-05-31 2025-12-16 Children's Hospital Medical Center Methods of generating and expanding hematopoietic stem cells
US12534709B2 (en) 2019-05-31 2026-01-27 Children's Hospital Medical Center Shaped organoid compositions and methods of making same
US12600943B2 (en) 2019-02-01 2026-04-14 The University Of Hong Kong Innervated organoid compositions and methods of making same

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2963704A1 (en) 2014-10-17 2016-04-21 Children's Hospital Medical Center In vivo model of human small intestine using pluripotent stem cells and methods of making and using same
CA3045145A1 (en) 2016-12-05 2018-06-14 Children's Hospital Medical Center Colonic organoids and methods of making and using same
WO2021060380A1 (ja) * 2019-09-27 2021-04-01 公立大学法人横浜市立大学 肝細胞の可塑性誘導法
KR20230010625A (ko) 2020-03-11 2023-01-19 비트 바이오 리미티드 간 세포 생성 방법
EP4244336A1 (en) * 2020-11-13 2023-09-20 Oslo Universitetssykehus HF Artifical liver organoids and methods of their production
CN112553339B (zh) * 2020-12-29 2024-08-09 广东南芯医疗科技有限公司 伊立替康个体化用药基因的指导方法及试剂盒
US20240368556A1 (en) * 2021-06-11 2024-11-07 Children’S Hospital Medical Center Liver organoid model for hyperbilirubinemia and methods of making and using same
JP2023020221A (ja) * 2021-07-30 2023-02-09 ウシオ電機株式会社 薬剤評価方法
CN114317439B (zh) * 2021-12-23 2024-04-16 北京基石生命科技有限公司 一种培养肿瘤类器官的方法
CN114891831A (zh) * 2022-01-14 2022-08-12 北京清华长庚医院 过表达wnt2基因的内皮细胞株及其构建方法和应用
CN115386535B (zh) * 2022-10-26 2023-02-03 天津外泌体科技有限公司 多谱系肝类器官模型及基于该模型的药物肝毒评价方法
CN121013904A (zh) 2023-03-30 2025-11-25 儿童医院医学中心 临床级类器官
EP4713433A2 (en) 2023-05-18 2026-03-25 Children's Hospital Medical Center Liver organoids with intrahepatic sympathetic nerves, and methods of use thereof
EP4731750A2 (en) 2023-06-23 2026-04-29 Children's Hospital Medical Center Methods of matrix-free suspension culture
WO2025072803A1 (en) 2023-09-29 2025-04-03 Children's Hospital Medical Center Ntrk2 signaling-mediated alveolar capillary injury and repair
CN117701492B (zh) * 2023-12-14 2025-05-06 首都医科大学 肝类器官的构建方法及其应用
WO2025212920A1 (en) 2024-04-03 2025-10-09 Children's Hospital Medical Center Multi-zonal liver organoids
WO2025217202A1 (en) 2024-04-08 2025-10-16 Children's Hospital Medical Center Bile duct organoid

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013511969A (ja) 2009-11-25 2013-04-11 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル 確定的内胚葉細胞の肝分化のための方法
JP2013521810A (ja) 2010-03-22 2013-06-13 セルアーティス アーベー Ｗｎｔシグナル経路の調節による多能性細胞から肝細胞様細胞への分化誘導と成熟
JP2013535201A (ja) 2010-07-29 2013-09-12 コーニンクレッカ ネザーランド アカデミー ヴァン ウェテンシャッペン 肝臓オルガノイド、その使用、およびそれを得るための培養方法
WO2015183920A2 (en) 2014-05-28 2015-12-03 Children's Hospital Medical Center Methods and systems for converting precursor cells into gastric tissues through directed differentiation
WO2016056999A1 (en) 2014-10-08 2016-04-14 Agency For Science, Technology And Research Methods of differentiating stem cells into liver cell lineages
JP2016514968A (ja) 2013-03-15 2016-05-26 オルガノボ，インク． 操作した肝臓組織、そのアレイ、およびそれを製造する方法
WO2016141137A1 (en) 2015-03-03 2016-09-09 President And Fellows Of Harvard College Methods of generating functional human tissue
WO2017048193A1 (en) 2015-09-15 2017-03-23 Agency For Science, Technology And Research (A*Star) Derivation of liver organoids from human pluripotent stem cells
WO2017153992A1 (en) 2016-03-08 2017-09-14 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd Method and system for continuous biosensing
WO2018085615A1 (en) 2016-11-04 2018-05-11 Children's Hospital Medical Center Liver organoid compositions and methods of making and using same

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TR201104076A1 (tr) * 2011-04-26 2012-11-21 Kordsa Global End�Str�Yel �Pl�K Ve Kord Bez� San. Ve T�C. A.�. Araç lastiği üst kuşak şeridinin üretim yöntemi.
JP6494515B2 (ja) * 2012-10-19 2019-04-03 エージェンシー フォー サイエンス， テクノロジー アンド リサーチ 幹細胞を１又は２以上の細胞系列に分化させる方法
US9324528B1 (en) * 2014-11-17 2016-04-26 General Electric Company Magnetic trip mechanism for circuit breaker
NZ759164A (en) * 2017-06-09 2023-02-24 Children’S Hospital Medical Center Liver organoid compositions and methods of making and using same

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013511969A (ja) 2009-11-25 2013-04-11 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル 確定的内胚葉細胞の肝分化のための方法
JP2013521810A (ja) 2010-03-22 2013-06-13 セルアーティス アーベー Ｗｎｔシグナル経路の調節による多能性細胞から肝細胞様細胞への分化誘導と成熟
JP2013535201A (ja) 2010-07-29 2013-09-12 コーニンクレッカ ネザーランド アカデミー ヴァン ウェテンシャッペン 肝臓オルガノイド、その使用、およびそれを得るための培養方法
JP2016514968A (ja) 2013-03-15 2016-05-26 オルガノボ，インク． 操作した肝臓組織、そのアレイ、およびそれを製造する方法
WO2015183920A2 (en) 2014-05-28 2015-12-03 Children's Hospital Medical Center Methods and systems for converting precursor cells into gastric tissues through directed differentiation
WO2016056999A1 (en) 2014-10-08 2016-04-14 Agency For Science, Technology And Research Methods of differentiating stem cells into liver cell lineages
WO2016141137A1 (en) 2015-03-03 2016-09-09 President And Fellows Of Harvard College Methods of generating functional human tissue
WO2017048193A1 (en) 2015-09-15 2017-03-23 Agency For Science, Technology And Research (A*Star) Derivation of liver organoids from human pluripotent stem cells
WO2017153992A1 (en) 2016-03-08 2017-09-14 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd Method and system for continuous biosensing
WO2018085615A1 (en) 2016-11-04 2018-05-11 Children's Hospital Medical Center Liver organoid compositions and methods of making and using same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Kyle W. McCracken et al.，Modelling human development and disease in pluripotent stem-cell-derived gastric organoids，NATURE，2014年，Vol.516，pp.400-404

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12258584B2 (en) 2010-05-06 2025-03-25 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
US12241090B2 (en) 2014-05-28 2025-03-04 Children's Hospital Medical Center Methods and systems for converting precursor cells into gastric tissues through directed differentiation
US12414967B2 (en) 2016-11-04 2025-09-16 Children's Hospital Medical Center Compositions and methods of treating liver disease
JP7545186B2 (ja) 2016-11-04 2024-09-04 チルドレンズ ホスピタル メディカル センター 肝臓オルガノイド組成物ならびにその作製および使用方法
JP2022119862A (ja) * 2016-11-04 2022-08-17 チルドレンズ ホスピタル メディカル センター 肝臓オルガノイド組成物ならびにその作製および使用方法
US12281334B2 (en) 2017-04-14 2025-04-22 Children's Hospital Medical Center Multi donor stem cell compositions and methods of making same
JP2022191263A (ja) * 2017-06-09 2022-12-27 チルドレンズ ホスピタル メディカル センター 肝臓オルガノイド組成物ならびにその作製および使用方法
JP7749290B2 (ja) 2017-06-09 2025-10-06 チルドレンズ ホスピタル メディカル センター 肝臓オルガノイド組成物ならびにその作製および使用方法
US12297457B2 (en) 2017-10-10 2025-05-13 Children's Hospital Medical Center Esophageal tissue and/or organoid compositions and methods of making same
US12379372B2 (en) 2017-12-21 2025-08-05 Children's Hospital Medical Center Digitalized human organoids and methods of using same
US12421500B2 (en) 2018-07-26 2025-09-23 Children's Hospital Medical Center Hepato-biliary-pancreatic tissues and methods of making same
US12428622B2 (en) 2018-09-12 2025-09-30 Children's Hospital Medical Center Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
US12600943B2 (en) 2019-02-01 2026-04-14 The University Of Hong Kong Innervated organoid compositions and methods of making same
US12497597B2 (en) 2019-05-31 2025-12-16 Children's Hospital Medical Center Methods of generating and expanding hematopoietic stem cells
US12534709B2 (en) 2019-05-31 2026-01-27 Children's Hospital Medical Center Shaped organoid compositions and methods of making same

Also Published As

Publication number Publication date
KR102781633B1 (ko) 2025-03-18
JP2020523000A (ja) 2020-08-06
CN110914408A (zh) 2020-03-24
JP7749290B2 (ja) 2025-10-06
EP3635095A4 (en) 2021-12-01
EP3635095A1 (en) 2020-04-15
AU2018279790A1 (en) 2019-12-05
WO2018226267A1 (en) 2018-12-13
CA3065759A1 (en) 2018-12-13
IL270714A (en) 2020-01-30
IL270714B2 (en) 2026-01-01
KR20240010095A (ko) 2024-01-23
IL270714B1 (en) 2025-09-01
US20200199537A1 (en) 2020-06-25
KR20200015898A (ko) 2020-02-13
NZ759164A (en) 2023-02-24
AU2022204804A1 (en) 2022-07-28
AU2018279790B2 (en) 2022-04-07
KR102625361B1 (ko) 2024-01-18
JP2022191263A (ja) 2022-12-27

Similar Documents

Publication Publication Date Title
JP7749290B2 (ja) 肝臓オルガノイド組成物ならびにその作製および使用方法
JP7545186B2 (ja) 肝臓オルガノイド組成物ならびにその作製および使用方法
US20250223567A1 (en) Compositions and methods for obtaining vascularized human intestinal organoid tissue, and related uses thereof
Wu et al. Modeling drug-induced liver injury and screening for anti-hepatofibrotic compounds using human PSC-derived organoids
EP4678727A2 (en) Hepato-biliary-pancreatic tissues and methods of making same
CN103180436A (zh) 体外肝分化
Shiraki et al. Efficient differentiation of embryonic stem cells into hepatic cells in vitro using a feeder-free basement membrane substratum
JP2024174893A (ja) 肝臓オルガノイド組成物ならびにその作製および使用方法
Harrison Liver cell types derived from pluripotent stem cells
Withey Differentiation of hepatocyte-like cells from human pluripotent stem cells

Legal Events

Date Code Title Description
A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20200825

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20200825

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210218

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20210218

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20220111

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20220406

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220427

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20220823

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20220922

R150 Certificate of patent or registration of utility model

Ref document number: 7148552

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

LAPS Cancellation because of no payment of annual fees