JP7093310B2 - 外因性ランディングパッドへの核酸のゲノム組込みのための組成物および方法 - Google Patents

外因性ランディングパッドへの核酸のゲノム組込みのための組成物および方法 Download PDF

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JP7093310B2
JP7093310B2 JP2018560571A JP2018560571A JP7093310B2 JP 7093310 B2 JP7093310 B2 JP 7093310B2 JP 2018560571 A JP2018560571 A JP 2018560571A JP 2018560571 A JP2018560571 A JP 2018560571A JP 7093310 B2 JP7093310 B2 JP 7093310B2
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sequence
landing pad
host cell
landing
genome
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JP2019516378A (ja
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ジョージ，ケビン
マイン，アンドリュー
ツァイ，チア－ホン
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アミリス， インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

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  • Bioinformatics & Computational Biology (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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JP2018560571A 2016-05-18 2017-05-18 外因性ランディングパッドへの核酸のゲノム組込みのための組成物および方法 Active JP7093310B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662338412P 2016-05-18 2016-05-18
US62/338,412 2016-05-18
PCT/US2017/033369 WO2017201311A2 (en) 2016-05-18 2017-05-18 Compositions and methods for genomic integration of nucleic acids into exogenous landing pads

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JP2019516378A JP2019516378A (ja) 2019-06-20
JP2019516378A5 JP2019516378A5 (https=) 2020-06-18
JP7093310B2 true JP7093310B2 (ja) 2022-06-29

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US (2) US11293033B2 (https=)
EP (2) EP4219731A3 (https=)
JP (1) JP7093310B2 (https=)
WO (1) WO2017201311A2 (https=)

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US11866726B2 (en) 2017-07-14 2024-01-09 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
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WO2024076688A2 (en) * 2022-10-05 2024-04-11 The Regents Of The University Of California Synthetic genomic safe harbors and methods thereof
CN120731271A (zh) * 2023-04-21 2025-09-30 诺维信公司 多拷贝宿主细胞的生成
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Also Published As

Publication number Publication date
EP4219731A2 (en) 2023-08-02
WO2017201311A2 (en) 2017-11-23
JP2019516378A (ja) 2019-06-20
US20220251606A1 (en) 2022-08-11
US20190144887A1 (en) 2019-05-16
US12110498B2 (en) 2024-10-08
EP4219731A3 (en) 2023-08-09
US11293033B2 (en) 2022-04-05
WO2017201311A3 (en) 2018-03-29
EP3458595A2 (en) 2019-03-27

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