JP7055805B2 - ナノポアを用いて検体を特徴付けるための方法及びシステム - Google Patents

ナノポアを用いて検体を特徴付けるための方法及びシステム Download PDF

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JP7055805B2
JP7055805B2 JP2019529497A JP2019529497A JP7055805B2 JP 7055805 B2 JP7055805 B2 JP 7055805B2 JP 2019529497 A JP2019529497 A JP 2019529497A JP 2019529497 A JP2019529497 A JP 2019529497A JP 7055805 B2 JP7055805 B2 JP 7055805B2
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polynucleotide
nanopore
strand
adapter
stranded
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JP2020500528A (ja
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クラーク，ジェームズ
ホワイト，ジェームズ
マスカット，リチャード
マリー メイ ノット，ジェシカ
イクバル ナサニ，ラミズ
ジョン ヘロン，アンドリュー
ジョン ブルース，マーク
ニシャンサ ジャヤシンゲ，ラクマル
キャプロッティ，ドメニコ
ジャクソン ストッダート，デヴィッド
ヴィクトリア ボーウェン，レベッカ
ジェームズ ライト，クリストファー
リチャード ムーディー，ポール
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オックスフォード ナノポール テクノロジーズ ピーエルシー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores

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JP2019529497A 2016-12-01 2017-11-29 ナノポアを用いて検体を特徴付けるための方法及びシステム Active JP7055805B2 (ja)

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GB1620450.5 2016-12-01
GBGB1620450.5A GB201620450D0 (en) 2016-12-01 2016-12-01 Method
US201762471338P 2017-03-14 2017-03-14
US62/471,338 2017-03-14
PCT/GB2017/053603 WO2018100370A1 (en) 2016-12-01 2017-11-29 Methods and systems for characterizing analytes using nanopores

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US (2) US11466317B2 (https=)
EP (2) EP3548636B1 (https=)
JP (1) JP7055805B2 (https=)
CN (3) CN110168104A (https=)
AU (1) AU2017367238B2 (https=)
CA (1) CA3044351A1 (https=)
GB (1) GB201620450D0 (https=)
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Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
EP2875128B8 (en) 2012-07-19 2020-06-24 Oxford Nanopore Technologies Limited Modified helicases
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
CN118256603A (zh) 2013-10-18 2024-06-28 牛津纳米孔科技公开有限公司 经修饰的酶
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
EP3971300B1 (en) 2014-10-16 2024-06-05 Oxford Nanopore Technologies plc Sorting of polymers
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
EP4063521B1 (en) 2016-05-25 2024-02-21 Oxford Nanopore Technologies PLC Method of nanopore sequencing
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB2569630B (en) 2017-12-21 2022-10-12 Sharp Life Science Eu Ltd Droplet Interfaces in Electro-wetting Devices
CN121472373A (zh) 2018-05-22 2026-02-06 安序源有限公司 用于核酸测序的方法、系统和组合物
WO2019227013A1 (en) 2018-05-24 2019-11-28 Oxford Nanopore Technologies Inc. Droplet interfaces in electro-wetting devices
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
GB2585816A (en) * 2018-12-12 2021-01-27 Univ York Proof-of-work for blockchain applications
CN111455031A (zh) * 2019-01-18 2020-07-28 中国科学院微生物研究所 基于Nanopore测序技术的多组学测序及分析方法
US11789006B2 (en) * 2019-03-12 2023-10-17 Oxford Nanopore Technologies Plc Nanopore sensing device, components and method of operation
GB201907243D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Sensing interactions between molecular entities and nanapores
CN114364797B (zh) * 2019-09-18 2023-10-20 株式会社日立高新技术 生物分子分析方法
KR20220148196A (ko) * 2020-02-10 2022-11-04 팔로젠 인코포레이티드 나노포어 디바이스 및 이를 사용하여 하전된 입자들을 검출하고 분류하는 방법
WO2021184374A1 (en) * 2020-03-20 2021-09-23 Genesense Technology Inc High throughput analytical system for molecule detection and sensing
US20230295712A1 (en) 2020-06-18 2023-09-21 Oxford Nanopore Technologies Plc A method of selectively characterising a polynucleotide using a detector
CN113930406B (zh) * 2021-12-17 2022-04-08 北京齐碳科技有限公司 一种Pif1-like解旋酶及其应用
AU2021306183A1 (en) * 2020-07-06 2023-01-05 Illumina Cambridge Limited Separating polynucleotide fragments
CN112309503A (zh) * 2020-10-19 2021-02-02 深圳市儒翰基因科技有限公司 基于纳米孔电信号的碱基判读方法、判读设备及存储介质
US12090477B2 (en) * 2021-03-05 2024-09-17 Taiwan Semiconductor Manufacturing Company Ltd. System and method for detecting biomolecules
WO2022243692A1 (en) * 2021-05-19 2022-11-24 Oxford Nanopore Technologies Plc Methods for complement strand sequencing
GB202107192D0 (en) * 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
CN113322180B (zh) * 2021-05-31 2022-07-15 中国科学院物理研究所 基于纳米孔测序的力谱分析方法和分析装置
CN115537413A (zh) * 2021-06-29 2022-12-30 中国农业大学 一种双链功能核酸纳米花的组装和形貌调控方法及其应用
CN113462764B (zh) * 2021-09-01 2021-12-17 北京齐碳科技有限公司 用于表征双链靶多核苷酸的类发夹衔接子、构建体和方法
WO2023055752A2 (en) 2021-09-28 2023-04-06 Axbio Inc. Methods for processing a nucleic acid sample and compositions thereof
CN114134142B (zh) * 2021-11-23 2024-05-28 成都齐碳科技有限公司 衔接体、复合物、单链分子、试剂盒、方法和应用
WO2023125605A1 (zh) * 2021-12-30 2023-07-06 深圳华大生命科学研究院 单分子纳米孔测序方法
CN118679255A (zh) * 2021-12-31 2024-09-20 深圳华大生命科学研究院 能够阻断马达蛋白的核酸分子及其构建方法和用途
EP4519453A1 (en) 2022-05-06 2025-03-12 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
AU2023270700A1 (en) 2022-05-17 2024-10-31 Oxford Nanopore Technologies Plc Method and adaptors
GB202207267D0 (en) 2022-05-18 2022-06-29 Oxford Nanopore Tech Plc Calibration and profiling of a nanopore array device
WO2024077281A2 (en) * 2022-10-06 2024-04-11 Cornell University Genotyping and sequencing assays for prrsv
GB202215442D0 (en) 2022-10-19 2022-11-30 Oxford Nanopore Tech Plc Analysis of a polymer
WO2024086745A2 (en) * 2022-10-21 2024-04-25 Axbio Inc. Systems and methods for analyzing a target molecule
WO2024101988A1 (en) 2022-11-11 2024-05-16 Umc Utrecht Holding B.V. Computer-implemented method for identifying a dna modification
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
CN116478983B (zh) * 2022-11-21 2023-10-24 北京普译生物科技有限公司 一种rna-dna嵌合接头及其应用
WO2024124567A1 (zh) * 2022-12-16 2024-06-20 深圳华大生命科学研究院 纳米孔测序方法和试剂盒
GB202301095D0 (en) 2023-01-25 2023-03-08 Oxford Nanopore Tech Plc Calibration of a nanopore array device
GB202304324D0 (en) 2023-03-24 2023-05-10 Oxford Nanopore Tech Plc Method and kits
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases
WO2025000432A1 (zh) * 2023-06-30 2025-01-02 深圳华大生命科学研究院 利用封闭子进行纳米孔测序的方法
WO2025223677A1 (en) * 2024-04-26 2025-10-30 Helmholtz-Zentrum für Infektionsforschung GmbH Method for analyzing molecules of interest, method for automatically selecting electrical signals related to a set of known barcode molecules, signal processing unit and computer program for executing the method
WO2026057755A1 (en) 2024-09-13 2026-03-19 Oxford Nanopore Technologies Plc Adaptors and kits for rna molecules labelling and characterising

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013185137A1 (en) 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
US20160041179A1 (en) 2013-03-15 2016-02-11 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
WO2016099673A1 (en) 2014-12-19 2016-06-23 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus

Family Cites Families (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US20120160687A1 (en) 1995-03-17 2012-06-28 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
WO2000034527A2 (en) 1998-12-11 2000-06-15 The Regents Of The University Of California Targeted molecular bar codes
WO2001059453A2 (en) * 2000-02-11 2001-08-16 The Texas A & M University System Biosensor compositions and methods of use
FR2814939B1 (fr) 2000-10-05 2002-12-20 Sofradim Production Ensemble de soutenement sous-uretral dans le traitement de l'incontinence urinaire d'effort de la femme
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US8278055B2 (en) * 2002-05-01 2012-10-02 Intel Corporation Methods and device for analyte characterization
US7238485B2 (en) 2004-03-23 2007-07-03 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
WO2008102120A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
US8698481B2 (en) 2007-09-12 2014-04-15 President And Fellows Of Harvard College High-resolution molecular sensor
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
JP2011527191A (ja) 2008-07-07 2011-10-27 オックスフォード ナノポア テクノロジーズ リミテッド 塩基検出細孔
WO2010004265A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Enzyme-pore constructs
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
US20120100530A1 (en) 2009-01-30 2012-04-26 Oxford Nanopore Technologies Limited Enzyme mutant
GB0901588D0 (en) 2009-02-02 2009-03-11 Itis Holdings Plc Apparatus and methods for providing journey information
CN102741430B (zh) 2009-12-01 2016-07-13 牛津楠路珀尔科技有限公司 生化分析仪器、用于进行生化分析的第一模块以及相关方法
US8586301B2 (en) 2010-06-30 2013-11-19 Stratos Genomics, Inc. Multiplexed identification of nucleic acid sequences
WO2012042226A2 (en) 2010-10-01 2012-04-05 Oxford Nanopore Technologies Limited Biochemical analysis apparatus and rotary valve
AU2012215135B9 (en) 2011-02-11 2017-03-09 Oxford Nanopore Technologies Limited Mutant pores
US9347929B2 (en) 2011-03-01 2016-05-24 The Regents Of The University Of Michigan Controlling translocation through nanopores with fluid wall
SG10201604316WA (en) * 2011-05-27 2016-07-28 Oxford Nanopore Tech Ltd Coupling method
AU2012288629B2 (en) 2011-07-25 2017-02-02 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
EP3663412B1 (en) 2011-09-23 2022-03-09 Oxford Nanopore Technologies PLC Analysis of a polymer comprising polymer units by means of translocation through a nanopore
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
US9617591B2 (en) 2011-12-29 2017-04-11 Oxford Nanopore Technologies Ltd. Method for characterising a polynucleotide by using a XPD helicase
BR112014016112B8 (pt) 2011-12-29 2022-12-27 Oxford Nanopore Tech Ltd Método e aparelho para caracterizar um polinucleotídeo alvo, kit, e, uso de uma helicase
JP6333179B2 (ja) 2012-01-20 2018-05-30 ジニア テクノロジーズ， インコーポレイテッド ナノポアベースの分子検出および配列決定
DK2814959T3 (en) 2012-02-17 2018-04-23 Hutchinson Fred Cancer Res COMPOSITIONS AND PROCEDURES FOR EXACTLY IDENTIFYING MUTATIONS
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
AU2013267609C1 (en) 2012-05-31 2019-01-03 Board Of Regents, The University Of Texas System Method for accurate sequencing of DNA
EP3674412A1 (en) * 2012-06-20 2020-07-01 The Trustees of Columbia University in the City of New York Nucleic acid sequencing by nanopore detection of tag molecules
EP2875128B8 (en) 2012-07-19 2020-06-24 Oxford Nanopore Technologies Limited Modified helicases
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
EP2911771B1 (en) 2012-10-26 2017-01-18 Oxford Nanopore Technologies Limited Droplet interfaces
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
EP2917732B1 (en) * 2012-11-09 2016-09-07 Stratos Genomics Inc. Concentrating a target molecule for sensing by a nanopore
US9605309B2 (en) * 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
KR102168813B1 (ko) 2013-03-08 2020-10-22 옥스포드 나노포어 테크놀로지즈 리미티드 효소 정지 방법
CN203450281U (zh) 2013-08-01 2014-02-26 大行科技(深圳)有限公司 一种折叠自行车走线结构
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CN118256603A (zh) 2013-10-18 2024-06-28 牛津纳米孔科技公开有限公司 经修饰的酶
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
JP6749243B2 (ja) 2014-01-22 2020-09-02 オックスフォード ナノポール テクノロジーズ リミテッド １つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法
WO2015127387A1 (en) 2014-02-21 2015-08-27 Northeastern University Fluorescence-based analysis of biopolymers using nanopores
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US20170096703A1 (en) 2014-03-21 2017-04-06 Oxford Nanopore Technologies Ltd. Analysis of a polymer from multi-dimensional measurements
EP3126515B1 (en) 2014-04-04 2018-08-29 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
WO2015176034A1 (en) 2014-05-15 2015-11-19 Two Pore Guys, Inc. Scaffold data storage and target detection in a sample using a nanopore
CN106687574B (zh) 2014-06-03 2021-06-29 亿明达股份有限公司 使用对纳米颗粒或纳米颗粒附近锚定的系链检测事件的组合物，系统和方法
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
EP3971300B1 (en) 2014-10-16 2024-06-05 Oxford Nanopore Technologies plc Sorting of polymers
KR102457147B1 (ko) * 2014-10-17 2022-10-19 옥스포드 나노포어 테크놀로지즈 피엘씨 나노 세공 rna 특징 규명을 위한 방법
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
AU2016220404B2 (en) * 2015-02-17 2021-05-27 Mgi Tech Co., Ltd. DNA sequencing using controlled strand displacement
WO2016161402A1 (en) 2015-04-03 2016-10-06 Abbott Laboratories Devices and methods for sample analysis
KR102713216B1 (ko) 2015-06-25 2024-10-02 로스웰 엠이 아이엔씨. 바이오분자 센서들 및 방법들
US20200216887A1 (en) 2016-01-21 2020-07-09 Genia Technologies, Inc. Nanopore sequencing complexes
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201620450D0 (en) * 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013185137A1 (en) 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
US20160041179A1 (en) 2013-03-15 2016-02-11 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
WO2016099673A1 (en) 2014-12-19 2016-06-23 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus

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EP4269617A2 (en) 2023-11-01
CN116334200B (zh) 2024-10-11
JP2020500528A (ja) 2020-01-16
WO2018100370A1 (en) 2018-06-07
US11466317B2 (en) 2022-10-11
AU2017367238B2 (en) 2021-10-21
US20190352709A1 (en) 2019-11-21
GB201620450D0 (en) 2017-01-18
EP3548636B1 (en) 2023-08-09
US20230084931A1 (en) 2023-03-16
CN116377044B (zh) 2025-05-06
CN116377044A (zh) 2023-07-04
CN110168104A (zh) 2019-08-23
AU2017367238A1 (en) 2019-06-13
AU2017367238A8 (en) 2019-07-18
EP3548636A1 (en) 2019-10-09
CN116334200A (zh) 2023-06-27
US12509721B2 (en) 2025-12-30
EP4269617A3 (en) 2024-01-10
CA3044351A1 (en) 2018-06-07

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