JP7044554B2 - 自動多重組織染色アッセイの間のアルカリホスファターゼ及びペルオキシダーゼ酵素の同時不活化のための組成物と方法 - Google Patents

自動多重組織染色アッセイの間のアルカリホスファターゼ及びペルオキシダーゼ酵素の同時不活化のための組成物と方法 Download PDF

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JP7044554B2
JP7044554B2 JP2017558986A JP2017558986A JP7044554B2 JP 7044554 B2 JP7044554 B2 JP 7044554B2 JP 2017558986 A JP2017558986 A JP 2017558986A JP 2017558986 A JP2017558986 A JP 2017558986A JP 7044554 B2 JP7044554 B2 JP 7044554B2
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ジェームス グリル，
ブライアン ディー． ケリー，
ジェローム コスミダー，
エリック メイ，
ノエミ セバスチャン，
パメラ ワース，
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ヴェンタナ メディカル システムズ， インク．
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    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)

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JP2017558986A 2015-05-10 2016-05-09 自動多重組織染色アッセイの間のアルカリホスファターゼ及びペルオキシダーゼ酵素の同時不活化のための組成物と方法 Active JP7044554B2 (ja)

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US201562159297P 2015-05-10 2015-05-10
US62/159,297 2015-05-10
PCT/EP2016/060263 WO2016180747A1 (en) 2015-05-10 2016-05-09 Compositions and methods for simultaneous inactivation of alkaline phosphatase and peroxidase enzymes during automated multiplex tissue staining assays

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JP2020205107A Division JP2021058193A (ja) 2015-05-10 2020-12-10 自動多重組織染色アッセイの間のアルカリホスファターゼ及びペルオキシダーゼ酵素の同時不活化のための組成物と方法

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JP2020205107A Pending JP2021058193A (ja) 2015-05-10 2020-12-10 自動多重組織染色アッセイの間のアルカリホスファターゼ及びペルオキシダーゼ酵素の同時不活化のための組成物と方法

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CN (1) CN107980095A (enExample)
AU (1) AU2016261279B2 (enExample)
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JP7455816B2 (ja) 2018-09-20 2024-03-26 ヴェンタナ メディカル システムズ， インク． クマリン系架橋試薬
EP3904383A4 (en) 2018-12-25 2022-08-17 Hanx Biopharmaceutics, Inc ANTI-OX40 MONOCLONAL ANTIBODY AND USE THEREOF
CN116783485A (zh) 2021-01-15 2023-09-19 文塔纳医疗系统公司 储存稳定的笼状半抗原
JP2024507677A (ja) 2021-01-25 2024-02-21 ベンタナ メディカル システムズ， インコーポレイテッド １つ又は複数の検出可能部分で標識された１つ又は複数のバイオマーカーを含む染色された生物学的検体
US20240177633A1 (en) 2021-03-30 2024-05-30 Sato Holdings Kabushiki Kaisha Label structure and label
WO2022223137A1 (en) 2021-04-18 2022-10-27 Ventana Medical Systems, Inc. Morphological marker staining
CN113552362B (zh) * 2021-07-23 2024-08-09 湖北百奥斯生物科技有限公司 一种新型信号放大的免疫荧光试剂盒
CN120359417A (zh) 2022-12-23 2025-07-22 文塔纳医疗系统公司 用于评估抗原呈递机制组分的材料和方法及其用途
WO2025014787A1 (en) 2023-07-07 2025-01-16 Ventana Medical Systems, Inc. Brightfield triplex immunohistochemistry assay for evaluating the colocalization of the er, pr, and ki-67 biomarkers in cells

Family Cites Families (17)

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DE3410400C2 (de) * 1984-03-21 1987-04-02 Titmus Eurocon Kontaktlinsen GmbH, 8750 Aschaffenburg Kontaktlinsenpflegesatz und Verfahren zur Pflege von Kontaktlinsen
US5225325A (en) * 1990-03-02 1993-07-06 Ventana Medical Systems, Inc. Immunohistochemical staining method and reagents therefor
US5595707A (en) 1990-03-02 1997-01-21 Ventana Medical Systems, Inc. Automated biological reaction apparatus
CN1282422A (zh) * 1997-10-21 2001-01-31 癌症研究行动技术有限公司 对细胞生长异常的测定
AU763354B2 (en) 1998-02-27 2003-07-17 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US20030211630A1 (en) 1998-02-27 2003-11-13 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US6582962B1 (en) 1998-02-27 2003-06-24 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
SE9804064D0 (sv) 1998-11-25 1998-11-25 A & Science Invest Ab Medicinal product and method for treatment of conditions affecting neural stem cells or progenitor cells
US20050176072A1 (en) * 2002-06-21 2005-08-11 Martin Michelle D. MTA1 is a predictive and prognostic factor in human breast cancer
US20040248223A1 (en) * 2003-06-05 2004-12-09 Idexx Laboratories, Inc. Reduction of interfering peroxidase activity in peroxidase based detection methods
WO2005033333A2 (en) * 2003-10-07 2005-04-14 Dako Denmark A/S Methods and compositions for the diagnosis of cancer
JP5733734B2 (ja) * 2009-02-19 2015-06-10 国立大学法人 千葉大学 成長因子に誘発されるユークロマチン化（ｅｕｃｈｒｏｍａｔｉｎｉｚａｔｉｏｎ）に必要な、ＳＲＣ−ファミリーチロシンキナーゼの核局在
EP3061463A1 (en) * 2009-04-08 2016-08-31 LipUm AB New methods for treatment of inflammatory diseases
EP2993478B1 (en) 2009-10-19 2022-08-24 Ventana Medical Systems, Inc. Device and method for slide caching
US9920324B2 (en) * 2011-06-01 2018-03-20 Intrexon Actobiotics Nv Polycistronic expression system for bacteria
SG2014011084A (en) 2011-09-09 2014-04-28 Ventana Med Syst Inc Imaging systems, cassettes, and methods of using the same
WO2013148498A1 (en) 2012-03-27 2013-10-03 Ventana Medical Systems, Inc. Signaling conjugates and methods of use

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CN107980095A (zh) 2018-05-01
CA2984701A1 (en) 2016-11-17
US20180120202A1 (en) 2018-05-03
WO2016180747A1 (en) 2016-11-17
US20220026319A1 (en) 2022-01-27
AU2016261279A1 (en) 2017-10-12
US11162877B2 (en) 2021-11-02
CA2984701C (en) 2023-09-26
AU2016261279B2 (en) 2020-12-10
US11971337B2 (en) 2024-04-30
JP2021058193A (ja) 2021-04-15
EP3295173B1 (en) 2025-11-05
US20240328910A1 (en) 2024-10-03
JP2018520647A (ja) 2018-08-02
EP3295173A1 (en) 2018-03-21

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