JP7026040B2 - 連続的にバイオプリントされた多層組織構造体 - Google Patents
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Description
本願は、2015年6月16日に出願された米国特許仮出願第62/180,174号、及び2016年3月8日に出願された米国特許仮出願第62/180,174号の利益を主張し、これらの全文を参照により本明細書に援用するものとする。
文献
・Cyrille Norotte, Francois S. Marga, Laura E. Niklason, and Gabor Forgacs, “Scaffold-free vascular tissue engineering using bioprinting”, Biomaterials, Vol. 30, 2009, pp. 5910-5917.
・Jin-yau Lai, Cheol Yong Yoon, James J. Yoo, Tina Wulf, and Anthony Atala, “Phenotypic and functional characteristics of in vivo tissue engineered smooth muscle from normal and pathological bladders”, Journal of Urology, Vol. 168, 2002, pp. 1853-1858.
・Byung-Soon Kim, and David J. Mooney “Scaffolds for engineering smooth muscle under cyclic mechanical strain conditions”, J. Biomech Eng, Vol. 122(3_, 2000, pp. 210-215.
特許
・米国特許公開第20140287960号
・米国特許公開第20130345794号
・米国特許公開第20140377232号
・米国特許第7368279号
凝固生体適合性マトリックスは、生細胞の生存を支える多様な天然ポリマーまたは合成ポリマー、たとえばアルギン酸、ラミニン、フィブリン、ヒアルロン酸、ポリ(エチレン)グリコール系のゲル、ゼラチン、キトサン、アガロース、またはそれらの組合せなどをどれでも含むことができる。好ましい実施形態では、凝固生体適合性マトリックスは、アルギン酸、またはプリントヘッドからの吐出中に瞬間的に凝固可能な好適な他の生体適合性ポリマーを含んでいる。さらに好ましい実施形態では、凝固生体適合性マトリックスは、各合成組織ファイバーの半径方向断面全体にわたって、アルギン酸の均質な組成を含む。
対象の半月板移植片に用いることができる哺乳類細胞型の非限定的例としては、平滑筋細胞、骨格筋細胞、心筋細胞、上皮細胞、内皮細胞、筋芽細胞、線維芽細胞、胚性幹細胞、間葉幹細胞、誘導多能性幹細胞、分化幹細胞、組織由来細胞、及びそれらの任意の組合せが挙げられる。細胞は、ドナー(同種異系)から、またはレシピエント(自家)から得ることができる。細胞はまた、株化培養細胞から、または遺伝子操作された細胞及び/または所望の遺伝子型もしくは表現型が得られるように操作された細胞から得ることもできる。
本発明の態様には、試験因子及び/または条件が哺乳類筋細胞の収縮機能に及ぼす影響を評価する方法が含まれ、該方法は、a)対象である合成生組織構造体に試験因子及び/または条件を接触させるステップ、及びb)因子及び/または条件が哺乳類筋細胞に及ぼす影響を、筋肉機能を評価することによって、たとえば寸法変化(たとえば該構造体の面積、直径及び/または長さ)を測定すること、ならびに/または細胞生存率、増殖、極性化、及び/もしくは遺伝子発現、たとえば平滑筋アクチン、カルポニンもしくはカルデスモンの発現、抗平滑筋細胞抗体(ASMA)の結合などの測定結果を分析することによって、決定するステップを含む。好ましい実施形態では、構造体は、筒/管を含み、決定するステップは、筒の直径及び/または外周の変化を測定することを含む。
本発明の態様には、対象の筋肉の少なくとも一部分を修復及び/または置換する方法が含まれる。本明細書で説明する合成筋肉ファイバー組織及び管のどれでも、それを必要とする対象に移植して、筋肉の修復及び/または再生を行うことができる。したがって、対象の筋肉の欠損を修復するかまたは筋肉の再生を促進する方法も、本明細書で提供される。一実施形態では、方法は、本明細書で説明する合成筋組織構造体を、筋肉の修復または再生を必要とする欠損部位に移植することを含む。
Claims (16)
- 試験因子及び/または試験条件が哺乳類筋細胞の収縮機能に及ぼす影響を評価する方法であって、
a)重複する層でバイオプリンターから吐出される連続したファイバーを含む合成生組織構造体を前記試験因子と接触させる及び/または該合成生組織構造体を試験条件に供するステップであって、ここで前記ファイバーは、哺乳類筋細胞を含む凝固生体適合性マトリックスであり、前記凝固生体適合性マトリックスは、前記ファイバーの構造完全性及び形状忠実度を堆積後も維持することができるものであり、そしてここで前記ファイバーの構造完全性及び形状忠実度は、各重複する層の間でも維持され、前記重複する層は構造上別個である、ステップ;及び
b)前記試験因子及び/または試験条件が前記哺乳類筋細胞に及ぼす影響を、前記構造体の寸法変化を測定することによって決定するステップを含んでいる、前記方法。 - 前記構造体は筒を含み、前記決定するステップは前記筒の面積、直径及び/または外周の寸法変化を測定することを含んでいる、請求項1に記載の方法。
- 前記構造体は平板を含み、前記決定するステップは、前記平板の寸法変化を測定することを含んでいる、請求項1に記載の方法。
- 前記ファイバーは、前記構造体の少なくとも3、4、5、6、7、8、9、又は10以上の層を通して連続的にバイオプリントされている、請求項1に記載の方法。
- 前記生体適合性マトリックスは、生理学的適合性がある、請求項1に記載の方法。
- 前記生体適合性マトリックスは、アルギン酸を含む、請求項1に記載の方法。
- 前記筋細胞は平滑筋細胞である、請求項1に記載の方法。
- 前記筋細胞は骨格筋細胞である、請求項1に記載の方法。
- 前記筋細胞は心筋細胞である、請求項1に記載の方法。
- 前記構造体は少なくとも1種の追加の細胞型を含んでいる、請求項1に記載の方法。
- 前記少なくとも1種の追加の細胞型は、前記ファイバー内に分散している、請求項10に記載の方法。
- 前記少なくとも1種の追加の細胞型は、前記ファイバーの外周に外側のまたは第2の層として配置されている、請求項10に記載の方法。
- 前記哺乳類筋細胞はヒト細胞である、請求項1~12のいずれか1項に記載の方法。
- 請求項1~13のいずれか1項に記載の方法に使用するための合成生組織構造体であって、請求項1~13のいずれか1項で定義される前記構造体は、筒又は平板を含み、前記ファイバーは前記筒又は平板を通して連続している、合成生組織構造体。
- 前記凝固生体適合性マトリックスは、各合成組織ファイバーの半径方向断面で全体にわたって均質である、請求項14に記載の合成生組織構造体。
- 前記凝固生体適合性マトリックスは、アルギン酸を、コラーゲン、フィブロネクチン、トロンボスポンジン、グリコサミノグリカン、デオキシリボ核酸、接着糖タンパク質及びエラスチンのうちの1つ以上と一緒に含んでいる、請求項14又は15に記載の合成生組織構造体。
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
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US201562180174P | 2015-06-16 | 2015-06-16 | |
US62/180,174 | 2015-06-16 | ||
US201662305422P | 2016-03-08 | 2016-03-08 | |
US62/305,422 | 2016-03-08 | ||
PCT/CA2016/050707 WO2016201577A1 (en) | 2015-06-16 | 2016-06-16 | Continuously bioprinted multilayer tissue structure |
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JP2018524022A JP2018524022A (ja) | 2018-08-30 |
JP7026040B2 true JP7026040B2 (ja) | 2022-02-25 |
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US (2) | US11746333B2 (ja) |
EP (2) | EP3310902B1 (ja) |
JP (1) | JP7026040B2 (ja) |
KR (1) | KR20180042220A (ja) |
CN (1) | CN108026508A (ja) |
AU (1) | AU2016279941A1 (ja) |
CA (1) | CA2989679C (ja) |
DK (1) | DK3310902T3 (ja) |
IL (1) | IL256306A (ja) |
RU (1) | RU2018101261A (ja) |
WO (1) | WO2016201577A1 (ja) |
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AU2016279941A1 (en) | 2015-06-16 | 2018-01-18 | Aspect Biosystems Ltd. | Continuously bioprinted multilayer tissue structure |
US10926000B2 (en) | 2016-05-13 | 2021-02-23 | Colorado School Of Mines | Deposition-conversion method for tunable calcium phosphate coatings on substrates and apparatus prepared thereof |
EP3482260B1 (en) | 2016-07-08 | 2023-08-30 | Cypre, Inc. | Apparatus for patterning hydrogels into multi-well plates |
US10596757B2 (en) * | 2016-12-06 | 2020-03-24 | Northeastern University | Three dimensional mineralization printer |
US10967570B2 (en) | 2018-01-18 | 2021-04-06 | Revotek Co., Ltd | Device for printing lumen tissue construct, method for using the same and 3D bioprinter |
US11311368B2 (en) | 2018-01-18 | 2022-04-26 | Revotek Co., Ltd | Device for printing lumen tissue construct, method for using the same and 3D bioprinter |
KR102083788B1 (ko) | 2018-09-04 | 2020-03-03 | 주식회사 티앤알바이오팹 | 인공 혈관 제조용 3d 프린팅 시스템 및 이를 이용한 인공 혈관의 제조 방법 |
BR112021019402A2 (pt) * | 2019-03-29 | 2021-12-28 | Tdbt Ip Inc | Substituições de tecidos e órgãos e métodos de fazer os mesmos |
KR102547044B1 (ko) * | 2019-09-22 | 2023-06-26 | 미아테크 쓰리디, 엘티디. | 배양된 근육 조직의 물리적 조작 |
US20240267751A1 (en) | 2019-10-01 | 2024-08-08 | Telefonaktiebolaget Lm Ericsson (Publ) | Group Signalling Aspects for NR-NR Dual Connectivity (NR-DC) and Reconfiguration |
KR102253724B1 (ko) | 2019-11-26 | 2021-05-20 | 주식회사 티앤알바이오팹 | 회전형 3d 프린팅 조형판 및 이를 포함하는 3d 프린터 |
KR102189959B1 (ko) | 2019-11-26 | 2020-12-11 | 주식회사 티앤알바이오팹 | 멀티 3d 프린팅 헤드 및 이를 포함하는 3d 프린터 |
KR102253727B1 (ko) | 2019-11-26 | 2021-05-20 | 주식회사 티앤알바이오팹 | 바이오 클린 벤치 시스템 |
US20230119663A1 (en) * | 2020-03-26 | 2023-04-20 | Osaka University | Muscle tissue produced by bioprinting |
US11559389B2 (en) * | 2020-05-05 | 2023-01-24 | International Business Machines Corporation | Bioprinted living tissue with therapy capability |
KR102525459B1 (ko) | 2021-01-22 | 2023-04-25 | 피피에스 주식회사 | Cps(cyber physical system)에 연결된 용량미달 불량품 식별 시스템 및 방법 |
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US7368279B2 (en) * | 2004-11-24 | 2008-05-06 | The Regents Of The University Of Michigan | Three dimensional bioengineered smooth muscle tissue and sphincters and methods therefor |
US20090263849A1 (en) * | 2006-04-21 | 2009-10-22 | Drexel University | Bioprinting Three-Dimensional Structure Onto Microscale Tissue Analog Devices for Pharmacokinetic Study and Other Uses |
US8691274B2 (en) | 2008-02-14 | 2014-04-08 | Wake Forest University Health Sciences | Inkjet printing of tissues and cells |
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JP2014531204A (ja) | 2011-09-12 | 2014-11-27 | オルガノボ,インク. | インビトロでの研究使用のための操作した組織、そのアレイ、およびその製造方法 |
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US20240158758A1 (en) | 2024-05-16 |
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CA2989679A1 (en) | 2016-12-22 |
EP3310902A1 (en) | 2018-04-25 |
US11746333B2 (en) | 2023-09-05 |
EP3310902B1 (en) | 2022-08-10 |
RU2018101261A (ru) | 2019-07-16 |
KR20180042220A (ko) | 2018-04-25 |
US20180171304A1 (en) | 2018-06-21 |
IL256306A (en) | 2018-02-28 |
EP3310902A4 (en) | 2018-12-12 |
EP4130243A1 (en) | 2023-02-08 |
CN108026508A (zh) | 2018-05-11 |
JP2018524022A (ja) | 2018-08-30 |
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