JP6960382B2 - Toothpaste composition - Google Patents

Description

The present invention relates to a toothpaste composition having an excellent biofilm formation inhibitory effect.

Usually, a toothpaste composition has a composition having an appropriate viscosity that can ensure a feeling of use such as easy placement on a toothbrush and easy adhesion to a tooth surface, in addition to good ejection property from a container. It is a product, and in order to impart such viscosity, a binder such as sodium alginate, carrageenan, xanthan gum, and sodium carboxymethyl cellulose is used. Various techniques have been developed for further exerting a desired effect on such a toothpaste composition, and a technique focusing on using carrageenan as a binder is also known.

It is known that carrageenan has three types of structures, κ type, ι type, and λ type. As a technique for using the κ type, for example, Patent Document 1 describes κ-carrageenan. An oral composition containing carrageenan has been disclosed, and attempts have been made to enhance its appearance, properties, and usability. Further, Patent Document 2 discloses a fluoride-blended oral composition in which κ-carrageenan and an alkali metal alginate salt are used in combination, which enhances storage stability while blending fluoride and provides a good usability. Bringing.

On the other hand, a technique using a plurality of types of carrageenan having different structures is also known. For example, in Patent Document 3, mixed carrageenan in which κ-carrageenan and ι-carrageenan are mixed in a specific amount is blended together with chlorhexidine salts and sodium monofluorophosphate in an attempt to ensure good shape retention and appearance. The toothpaste composition is disclosed. Further, Patent Document 4 describes plaque suppression containing a polysaccharide selected from the group consisting of xanthan gum, tragacant gum, guar gum, karaya gum, chondroitin sulfate, polygalacturonic acid, sodium alginate, and kappa / lambda type carrageenan as an active ingredient. Oral compositions for this purpose are disclosed.

Japanese Unexamined Patent Publication No. 2004-300120 Japanese Unexamined Patent Publication No. 56-115711 JP-A-54-11243 Japanese Unexamined Patent Publication No. 1-213222

However, among the binders, carrageenan in particular tends to exhibit excessive viscosity, and it is also known that the amount of carrageenan used must be limited when it is blended into the dentifrice composition. ..
Under these circumstances, in order to obtain a toothpaste composition that more effectively suppresses the formation of a biofilm due to plaque, tongue coating, etc. in the oral cavity, Patent Documents 1 to 3 describe κ-carrageenan. However, since it is only an attempt to improve storage stability and usability while suppressing liquid separation, further studies are required. On the other hand, Patent Document 4 states that "xanthan gum, guar gum, and tragacant gum are the most effective of the tests, and suppress aggregation by 50% at concentrations of 0.05%, 0.15%, and 0.35%, respectively. It worked. "And" The highest concentration tested was 0.5% xanthan, which was too concentrated to be evaluated for aggregation by either method. " It is clear that there is still insufficient research to effectively suppress the formation of biofilms.

Under such circumstances, the present inventor has found that when κ-carrageenan is used under specific conditions among carrageenans, it exhibits an extremely useful biofilm formation inhibitory effect.
That is, the present invention relates to a toothpaste composition capable of exerting an excellent biofilm formation inhibitory effect while maintaining a good usability.

Therefore, as a result of diligent studies to solve the above problems, the present inventor unexpectedly used a specific amount of κ-carrageenan as a binder and a specific mass ratio with a specific anionic surfactant. It has been found that when used in combination, a carrageenan composition that exhibits an excellent biofilm formation inhibitory effect and maintains a good usability can be obtained.

Therefore, the present invention describes the following components (A) and (B):
(A) Binder containing κ-carrageenan (a1) (B) Containing one or more anionic surfactants selected from alkyl sulfates and N-acylamino acid salts.
The content of the component (a1) in the composition is 1.2% by mass or more and 7% by mass or less, and the mass ratio of the content of the component (a1) to the content of the component (B) in the composition ( (A1) / (B)) provides a toothpaste composition having a mass ratio of 0.25 or more and 5 or less.

According to the toothpaste composition of the present invention, a specific amount of κ-carrageenan is used in combination with a specific anionic surfactant in a specific mass ratio to effectively and effectively exert an effect of suppressing the formation of a biofilm. Not only can it be used, but it can also maintain an appropriate viscosity without excessive thickening, and it has an excellent usability that can exhibit good shape retention, discharge from a container, and adhesion to the tooth surface. It is possible to bring.

FIG. 1 is a bar graph showing the results of "verification of the biofilm formation inhibitory effect due to the difference in the type of carrageenan" in Test Example 1. The vertical axis represents the biofilm formation inhibition rate (%), and the horizontal axis represents the control and the type of each carrageenan dissolved in the surfactant water. FIG. 2 is a bar graph showing the results of "verification of the biofilm formation inhibitory effect due to the difference in the type of anionic surfactant" in Test Example 2. The vertical axis represents the biofilm formation inhibition rate (%), and the horizontal axis represents the control and the type of each anionic surfactant dissolved in κ-carrageenan water.

Hereinafter, the present invention will be described in detail.
The toothpaste composition of the present invention contains κ-carrageenan of component (a1) as a binder of component (A). As shown in FIG. 1, there are three types of carrageenan, which have been known to be used as binders, κ-carrageenan, ι-carrageenan, and λ-carrageenan, which have different positions and numbers of sulfate groups. , Κ-Carrageenan, when used in combination with an anionic surfactant, has been found to exert an excellent biofilm formation inhibitory effect, which is not found in other types, and is used in the present invention. It is a thing.

In the toothpaste composition of the present invention, the κ-carrageenan of the component (a1) forms a complex-like structure on the tooth surface and the like together with the specific anionic surfactant of the component (B), and is an excellent biofilm. It is presumed that it contributes to the exertion of the formation inhibitory effect.

The content of κ-carrageenan of the component (a1) is 1.2% by mass or more, preferably 1.7 in the toothpaste composition of the present invention, from the viewpoint of ensuring the effect of suppressing the formation of biofilm. It is mass% or more, and more preferably 2.5 mass% or more. Further, the content of κ-carrageenan of the component (a1) is 7% by mass or less, preferably 5% by mass or less in the toothpaste composition of the present invention from the viewpoint of ensuring an appropriate viscosity. , More preferably 3.6% by mass or less, still more preferably 3.2% by mass or less.

Further, the content of κ-carrageenan of the component (a1) is preferably 70% by mass or more in 100% by mass of the binder of the component (A) from the viewpoint of effectively enhancing the effect of suppressing the formation of the biofilm. , More preferably 80% by mass or more, still more preferably 90% by mass or more, still more preferably 93% by mass or more, still more preferably 95% by mass or more, still more preferably 96% by mass. The above is more preferably 98% by mass or more. Further, the content of κ-carrageenan of the component (a1) may be 100% by mass in 100% by mass of the binder of the component (A) from the viewpoint of ensuring the effect of suppressing the formation of the biofilm. From the viewpoint of imparting an appropriate viscosity, it may be 98% by mass or less.

The component (a1) is added to the toothpaste composition in a state of exhibiting a powdery appearance from the viewpoint of obtaining a toothpaste composition exhibiting a more excellent biofilm formation suppressing effect and from the viewpoint of ensuring an appropriate viscosity. It is preferable to mix.

From the viewpoint of ensuring an excellent biofilm formation inhibitory effect as a binder of the component (A) and maintaining an excellent usability, in addition to the κ-carrageenan of the component (a1), the component (a2) It is preferable to contain xanthan gum. Such xanthan gum can synergistically enhance the biofilm formation inhibitory effect of the component (a1) while maintaining good shape retention and dischargeability from the container.

The content of xanthan gum of the component (a2) is a binder of the component (A) from the viewpoint of achieving both an excellent effect of suppressing the formation of a biofilm and a good shape retention and a retention of a discharge property from a container. In 100% by mass, it is preferably 2% by mass or more, more preferably 4% by mass or more, further preferably 5% by mass or more, preferably 30% by mass or less, and more preferably 10% by mass. It is less than or equal to, more preferably 7% by mass or less, further preferably 5% by mass or less, further preferably 4% by mass or less, still more preferably 2% by mass or less.

The binder of the component (A) includes κ-carrageenan of the component (a1) and xanthan gum of the component (a2), and a carboxyvinyl polymer as another binder as long as the effect of the present invention is not impaired. , Sodium alginate, sodium polyacrylate, hydroxyethyl cellulose, hydroxypropyl cellulose, pectin, tragant gum, arabic gum, guar gum, carrageenan gum, locust bean gum, gellan gum, tamarint gum, psyllium seed gum, polyvinyl alcohol, sodium chondroitin sulfate, and methoxyethylene anhydrous One or more selected from maleic acid copolymer and the like may be appropriately contained.

The binder of the component (A) limits the content of sodium carboxymethyl cellulose of the component (a3) from the viewpoint of achieving both an excellent effect of suppressing the formation of a biofilm and imparting an appropriate viscosity. Is preferable. Specifically, the content of sodium carboxymethyl cellulose is preferably less than 15% by mass, more preferably less than 10% by mass, and even more preferably 5 in 100% by mass of the binder of the component (A). It is preferably less than mass%, or in the kneading toothpaste composition of the present invention, sodium carboxymethyl cellulose is not contained in the binder of the component (A).

The content of the component (A) is preferably 1.2% by mass or more, more preferably 1.5% by mass or more in the toothpaste composition of the present invention from the viewpoint of exerting an excellent biofilm formation suppressing effect. It is 1% by mass or more, more preferably 1.7% by mass or more, particularly preferably 2% by mass or more, and particularly preferably 2.5% by mass or more. Further, the content of the component (A) is preferably 10% by mass or less, more preferably 7% by mass or less, and further preferably 7% by mass or less in the toothpaste composition of the present invention from the viewpoint of ensuring an appropriate viscosity. It is preferably 5% by mass or less, and more preferably 3.5% by mass or less.

The toothpaste composition of the present invention contains one or more anionic surfactants selected from alkyl sulfates and N-acylamino acid salts as the component (B). By containing the anionic surfactant of the component (B) in an appropriate content ratio with respect to the component (a1), a film-like complex such as a sea-island structure can be formed on the tooth surface in combination with the above component (a1). It is possible to form a structure and further effectively enhance the effect of suppressing the formation of a biofilm.
The alkyl sulfate is preferably a linear or branched alkyl sulfate having 6 to 30 carbon atoms, more preferably a linear or branched alkyl sulfate having 8 to 24 carbon atoms, and further preferably having 8 to 24 carbon atoms. 8 to 18 linear or branched alkyl sulphates. Specifically, for example, one or more selected from sodium lauryl sulfate, sodium myristyl sulfate, sodium palmityl sulfate, sodium stearyl sulfate, sodium octyl sulfate, and sodium decyl sulfate can be mentioned. Among them, one or more selected from sodium myristyl sulfate and sodium lauryl sulfate are preferable from the viewpoint of effectively exerting a synergistic biofilm formation inhibitory effect with the component (a1).

As the acyl group of the N-acyl amino acid salt, a saturated or unsaturated linear or branched chain having 4 to 30 carbon atoms is used from the viewpoint of effectively exerting a synergistic biofilm formation inhibitory effect with the component (a1). It is preferably derived from a fatty acid having 6 to 26 carbon atoms, more preferably derived from a fatty acid having a saturated or unsaturated linear or branched chain having 6 to 26 carbon atoms, and saturated or unsaturated having 8 to 24 carbon atoms. Those derived from fatty acids having a straight chain or a branched chain are more preferable.

Examples of such fatty acids include one or more selected from caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid and the like. Among these fatty acids, one or more selected from lauric acid, myristic acid, palmitic acid, and oleic acid are preferable, and myristic acid is more preferable, from the viewpoint of ensuring good storage stability. Further, the acyl group of the N-acyl amino acid may be one derived from the above-mentioned mixed fatty acid of fatty acid, for example, one obtained from coconut oil, palm kernel oil or the like as a raw material. Among them, those derived from coconut oil fatty acid or palm kernel fatty acid are preferable, and those derived from coconut oil fatty acid are more preferable.

As the amino acid portion of the N-acylamino acid, glutamic acid, aspartic acid, glycine, alanine, threonine, methylalanine, sarcosine, and lysine are used from the viewpoint of effectively exerting a synergistic biofilm formation inhibitory effect with the component (a1). And one or more selected from arginine are preferred. Among them, the amino acid portion of the N-acyl amino acid is preferably an acidic amino acid, more preferably glutamic acid and aspartic acid, and even more preferably glutamic acid. The N-acylamino acid salt includes L-form, D-form, and racemic form, and may be any of these.

Examples of the salt of N-acylamino acid salt include alkali metal salts such as sodium and potassium; alkaline earth metal salts such as calcium and magnesium; other inorganic salts such as aluminum and zinc; ammonium salts; monoethanolamine, diethanolamine and tri. Organic amine salts such as ethanolamine; one or more selected from basic amino acid salts such as arginine, lysine, histidine, ornithine and the like can be mentioned. Among them, as the salt of the N-acylamino acid salt, an alkali metal salt is preferable, and a sodium salt is more preferable, from the viewpoint of flavor and availability.

Specific examples of the N-acylamino acid salt include sodium N-acylsarcosate, sodium N-acylglutamate, sodium N-acylalanine and the like, and more specifically, sodium N-lauroyl sarcosinate and N-. Sodium N-C _{10-16 acyl sarcosinate such as sodium myristyl sarcosinate; sodium NC 10-16} acyl glutamate such as sodium N-lauroyl glutamate, sodium N-myristoyl glutamate, sodium N-palmitoyl glutamate; sodium N-lauroylalanine Examples thereof include _{NC 10-16} acylalanine sodium such as sodium and N-myristoylalanine sodium.

The content of the component (B) is preferably 0.25% by mass or more in the toothpaste composition of the present invention from the viewpoint of effectively enhancing the biofilm formation suppressing effect in combination with the component (a1). It is preferably 0.6% by mass or more, and more preferably 1% by mass or more. Further, the content of the component (B) is preferably 3% by mass or less, preferably 2% by mass or less, in the toothpaste composition of the present invention, from the viewpoint of maintaining a good usability. It is preferably 1.8% by mass or less, and more preferably 1.6% by mass or less.

The mass ratio ((a1) / (B)) of the content of the component (a1) to the content of the component (B) in the toothpaste composition of the present invention depends on the component (a1) and the component (B). From the viewpoint of ensuring that the biofilm formation inhibitory effect is synergistically enhanced, it is 0.25 or more, preferably 0.5 or more, more preferably 1 or more, and further preferably 1.3 or more. It is particularly preferably 1.8 or more, 5 or less, preferably 4 or less, more preferably 3.5 or less, and further preferably 2.5 or less.

The toothpaste composition of the present invention may contain one or more organic acids selected from malic acid, phytic acid, citric acid and salts thereof or salts thereof as the component (C). The component (C) can enhance the dispersibility of the component (a1) with the component (B) and synergistically and effectively improve the biofilm formation inhibitory effect.

Examples of the salts of malic acid and citric acid include sodium salts and potassium salts, and sodium salts are preferable.
Phytic acid, also known as myo-inositol hexaphosphate, is an inositol phosphate ester compound. Examples of the phytic acid salt include alkali metal salts such as sodium and potassium, ammonium salts, and the like, and alkali metal salts are particularly preferable.

As the component (C), the viewpoint of more effectively enhancing the dispersibility of the component (a1) with the component (B) to synergistically and effectively improve the biofilm formation suppressing effect, as well as the taste and usability, etc. From the viewpoint of the above, one or more selected from malic acid and citric acid is preferable, and malic acid is more preferable.

The content of the component (C) is the formulation of the present invention from the viewpoint of effectively enhancing the dispersibility of the component (a1) with the component (B) and synergistically and effectively improving the biofilm formation suppressing effect. In the toothpaste composition, in terms of acid conversion amount, it is preferably 0.5% by mass or more, more preferably 1% by mass or more, still more preferably 1.5% by mass or more, and preferably 5% by mass. It is less than or equal to, more preferably 4% by mass or less, still more preferably 2.5% by mass or less.

The molar ratio ((a1) / (C)) of the content of the component (a1) to the content of the component (C) in the toothpaste composition of the present invention effectively enhances the dispersibility of the component (a1). From the viewpoint of ensuring an excellent biofilm formation inhibitory effect while maintaining an appropriate viscosity, it is preferably 0.00001 or more, more preferably 0.00005 or more, and even more preferably 0. It is 0001 or more, preferably 0.01 or less, and more preferably 0.005 or less.

The toothpaste composition of the present invention preferably contains water as the component (D). The water of the component (D) in the present invention is contained not only in purified water or the like blended in the toothpaste composition, but also in each of the blended components such as a 70% sorbitol solution (aqueous solution) used when prescribing. It means the total water contained in the toothpaste composition, including water. By containing such a component (D), each component can be satisfactorily dissolved or dispersed and diffused well in the oral cavity while maintaining good shape retention.

The content of the component (D) in the toothpaste composition of the present invention is preferably 5% by mass or more, more preferably 10% by mass or more, still more preferably 15% by mass or more, and even more. It is preferably 25% by mass or more, preferably 60% by mass or less, more preferably 50% by mass or less, still more preferably 45% by mass or less, still more preferably 40% by mass or less.
The water content of the component (D) can be calculated from the blended water content and the water content in the blended component, but can be measured by, for example, a Karl Fischer titer. As the Karl Fischer titer, for example, a trace moisture measuring device (Hiranuma Sangyo Co., Ltd.) can be used. In this device, 5 g of the toothpaste composition is taken, suspended in 25 g of anhydrous methanol, and 0.02 g of this suspension can be separated to measure the water content.

In the toothpaste composition of the present invention, magnesium and aluminum are used from the viewpoint of sufficiently exerting an excellent biofilm formation inhibitory effect by the complex-like structure formed by the component (a1) and the component (B). , It is preferable to limit the content of one or more polyvalent metals selected from iron, zinc and tin.

The content of one or more polyvalent metals selected from these magnesium, aluminum, iron, zinc and tin is preferably 0.5 mass in terms of metal atom equivalent in the toothpaste composition of the present invention. %, More preferably less than 0.1% by weight, even more preferably less than 0.01% by weight, or the toothpaste compositions of the present invention are made from these magnesium, aluminum, iron, zinc and tin. It is preferable that it does not contain one or more polyvalent metals selected.

The toothpaste composition of the present invention preferably further contains a sugar alcohol from the viewpoint of effectively exerting the effect of suppressing the formation of a biofilm. Examples of such sugar alcohols include one or more selected from sorbitol, xylitol, erythritol, reduced palatinose and mannitol. Among them, one or two selected from sorbitol and xylitol are more preferable from the viewpoint of having high solubility in water and giving the composition a smooth feel.
The content of such sugar alcohol in the toothpaste composition of the present invention is preferably 1% by mass or more, more preferably 5% by mass or more, still more preferably 10% by mass or more, and preferably 60% by mass. It is 5% by mass or less, more preferably 55% by mass or less, and further preferably 50% by mass or less.

The toothpaste composition of the present invention further comprises a surfactant other than the component (B); a fluorine ion supplying compound such as sodium fluoride, potassium fluoride, ammonium fluoride, etc., as long as the effect of the present invention is not impaired. Fluorine-containing compounds such as sodium monofluorophosphate; wetting agents and sweeteners such as glycerin, polyethylene glycol and propylene glycol; pH adjusters such as sodium hydrogen phosphate; fragrances; quaternary ammonium compounds, biguanide compounds, isopropylmethylphenol , Triclosan, Timor and β-glycyrrhetinic acid and other medicinal ingredients and other active ingredients; pigments and the like can be contained.

The B8H viscosity of the toothpaste composition of the present invention at 25 ° C. is preferably 800 dPa · s or more from the viewpoint of maintaining good shape retention, dischargeability from a container, etc. while exhibiting an excellent biofilm formation suppressing effect. It is more preferably 1000 dPa · s or more, further preferably 1100 dPa · s or more, further preferably 1200 dPa · s or more, still more preferably 1700 dPa · s or more, and preferably 4000 dPa · s or more. It is less than or equal to, preferably 3500 dPa · s or less, more preferably 2800 dPa · s or less, and more preferably 2500 dPa · s or less.
The B8H viscosity means a value measured using a B-type viscometer (TVB-10 type viscometer Toki Sangyo), and specifically, is static at room temperature (25 ° C.) for at least 24 hours after production. The placed viscometer composition can be measured under the conditions of rotor H-7, rotation speed 2.5 rpm, and 1 minute.

The method of applying the toothpaste composition of the present invention into the oral cavity may be either application or brushing with a toothbrush.
The oral composition of the present invention can also be used as a biofilm formation inhibitor.

The oral composition of the present invention is prepared in advance from the viewpoint of obtaining a toothpaste composition that enhances the dispersibility of the component (B) and the component (a1) and exerts a more excellent biofilm formation suppressing effect. It is preferable to obtain by a production method including a step of blending and mixing B) and the component (a1) and then appropriately blending other components. At this time, the dispersibility of the component (B) and the component (a1) is improved. From the viewpoint of further enhancing, it is preferable to use a component (a1) having a powdery appearance.

Hereinafter, the present invention will be specifically described based on examples. Unless otherwise specified in the table, the content of each component indicates mass%.

[Test Example 1: Verification of biofilm formation inhibitory effect by different types of carrageenan]
Soagina MV101 (Mitsubishi Chemical Foods Co., Ltd.) is used for κ-carrageenan, MV201 (Mitsubishi Chemical Foods Co., Ltd.) is used for ι-carrageenan, and ML200 (Mitsubishi Chemical Foods Co., Ltd.) is used for λ-carrageenan. A test solution was prepared by mixing and dissolving 2% by mass and 2% by mass of sodium lauryl sulfate in ion-exchanged water.
Next, as a tooth model, a pellet plate (apatite pellet: manufactured by HOYA Co., Ltd.) (hereinafter abbreviated as HAP plate) obtained by compressing hydroxyapatite powder, which is the main component of enamel, into a flat plate of about 1 cm square was used. A HAP plate treated with saliva collected from humans at 37 ° C. for 90 minutes was immersed in 1 mL of a solution of the test solution in ion-exchanged water (25% by mass of the test solution) for 2 minutes. The HAP plate was washed with 1 mL of ion-exchanged water, then immersed in a culture solution (sucrose: 5% by mass) in which sucrose was added to saliva collected from humans, and cultured at 37 ° C. for 1 day under anaerobic conditions.
After culturing, the biofilm on the surface of the HAP plate is stained with a plaque stain, and the stained area is image-processed (average value after color separation by RBG: measuring device VH-7000 (manufactured by KEYENCE): analysis software WIN. ROOF (manufactured by Mitani)) was used to quantify, and the biofilm formation inhibition rate (%) for each test solution was determined. Purified water in which both the carrageenan concentration and the surfactant concentration were 0% by mass was used as a control.
The results are shown in FIG.
From the above results, it was confirmed that in the presence of the component (B), κ-carrageenan exerts an excellent biofilm formation inhibitory effect unlike ι-carrageenan and λ-carrageenan.

[Test Example 2: Verification of biofilm formation inhibitory effect by different types of anionic surfactant]
Sodium lauryl sulfate, sodium myristyl sulfate, sodium myristyl glutamate, sodium lauroyl glutamate as anionic surfactants, cocamidopropyl betaine as a nonionic surfactant, κ-carrageenan 2% by mass and 2% by mass of each surfactant % And dissolved in ion-exchanged water to prepare a test solution.
Next, the same experiment as in Test Example 1 was carried out, and the biofilm formation inhibition rate (%) for each test solution was determined. The control had a surfactant concentration of 0% by mass.
The results are shown in FIG.
From the above results, in the presence of the component (a1), when a specific anionic surfactant was used, it exhibited an excellent biofilm inhibitory effect on a single solution of κ-carrageenan, and a carbon chain chain. Based on the length, it was confirmed that it exerts a more excellent biofilm formation inhibitory effect.

[Examples 1 to 9, Comparative Examples 1 to 2]
After heating and mixing the water-soluble components (excluding the components (a1) and (B)) according to the formulations shown in Tables 1 to 5, the powder raw material and the component (a1), the component (B) and the fragrance component are mixed. As a result, each toothpaste composition was obtained. Using the obtained toothpaste composition, evaluation and measurement were carried out according to the following method. In each of the toothpaste compositions, the content of one or more polyvalent metals selected from magnesium, aluminum, iron, zinc and tin was less than 0.01% by mass.
The results are shown in Tables 1-5.

<< Evaluation of biofilm formation inhibitory effect >>
The above-mentioned HAP plate was used as a tooth model. First, each of the obtained toothpaste compositions was dissolved in ion-exchanged water to prepare each test solution (toothpaste composition: 25% by mass). Next, HAP treated at 37 ° C. for 90 minutes was immersed in saliva collected from humans for 2 minutes in 1 mL of the obtained test solution. The HAP plate was washed with 1 mL of ion-exchanged water, then immersed in a culture solution (sucrose: 5% by mass) in which sucrose was added to saliva collected from humans, and cultured at 37 ° C. for 1 day under anaerobic conditions.
After culturing, the biofilm on the surface of the HAP plate is stained with a plaque stain, and the stained area is image-processed (average value after color separation by RBG: measuring device VH-7000 (manufactured by KEYENCE): analysis software WIN. Quantified by ROOF (manufactured by Mitani)). The amount of biofilm formed was calculated based on the amount of biofilm formed on the surface of HAP when treated by ion-exchanged water treatment instead of the test solution (100%).

《Measurement of viscosity》
The manufactured toothpaste composition is packed in a container for measuring viscosity, stored in an incubator at 25 ° C. for 24 hours, and then used with a B-type viscometer (TVB-10 type viscometer Toki Sangyo) to rotor H-. 7. The measurement was carried out under the conditions of a rotation speed of 2.5 rpm and 1 minute.

From the above results, by using a specific amount of κ-carrageenan with a specific anionic surfactant in a specific mass ratio under specific conditions, a toothpaste composition exhibiting an excellent biofilm formation inhibitory effect can be obtained. It was confirmed that

Claims (10)

The following components (A) , (B) and (C) :
(A) Binder containing κ-carrageenan (a1) (B) One or more anionic surfactants selected from alkyl sulfates and N-acylamino acid salts
(C) Contains one or more organic acids selected from malic acid, phytic acid, citric acid and salts thereof, or salts thereof .
The content of the component (a1) is 80% by mass or more in 100% by mass of the component (A).
The content of the component (a1) in the composition is 1.2% by mass or more and 5% by mass or less, and the mass ratio of the content of the component (a1) to the content of the component (B) in the composition ( (a1) / (B)) is Ri der 0.25 to 5, (molar ratio between the content and the content of the component (C) of a1) ((a1) component in the composition / (C) ) is Ru der 0.00001 0.01 or less, toothpaste compositions. The toothpaste composition according to claim 1 , wherein the content of the component (C) is 0.5% by mass or more and 5% by mass or less in terms of acid equivalent. The toothpaste composition according to claim 1 or 2 , which contains 5% by mass or more and 60% by mass or less of water (D). The toothpaste composition according to any one of claims 1 to 3 , wherein the content of sodium carboxymethyl cellulose (a3) is less than 15% by mass in 100% by mass of the component (A). Any one of claims 1 to 4 , wherein the content of one or more polyvalent metals selected from magnesium, aluminum, iron, zinc and tin is less than 0.5% by mass in terms of metal atom equivalent. The zinc paste composition according to the section. The toothpaste composition according to any one of claims 1 to 5 , wherein the content of the component (B) is 0.25% by mass or more and 2% by mass or less. The toothpaste composition according to any one of claims 1 to 6 , which comprises a component (a1) having a powdery appearance. The toothpaste composition according to any one of claims 1 to 7 , wherein the component (A) contains xanthan gum (a2). The toothpaste composition according to claim 8 , wherein the content of the component (a2) is 2% by mass or more and 30% by mass or less in 100% by mass of the component (A). The toothpaste composition according to any one of claims 1 to 9 , which is a biofilm formation inhibitor.

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