JP6930910B2 - Rna転写産物バリアントを定量するための方法及び製品 - Google Patents

Rna転写産物バリアントを定量するための方法及び製品 Download PDF

Info

Publication number
JP6930910B2
JP6930910B2 JP2017501008A JP2017501008A JP6930910B2 JP 6930910 B2 JP6930910 B2 JP 6930910B2 JP 2017501008 A JP2017501008 A JP 2017501008A JP 2017501008 A JP2017501008 A JP 2017501008A JP 6930910 B2 JP6930910 B2 JP 6930910B2
Authority
JP
Japan
Prior art keywords
artificial
sequence
family
transcript
molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017501008A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017525341A (ja
Inventor
パウル ルーカス
パウル ルーカス
クバラ ペトラ
クバラ ペトラ
レダ トルステン
レダ トルステン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lexogen GmbH
Original Assignee
Lexogen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lexogen GmbH filed Critical Lexogen GmbH
Publication of JP2017525341A publication Critical patent/JP2017525341A/ja
Priority to JP2021092115A priority Critical patent/JP7568581B2/ja
Application granted granted Critical
Publication of JP6930910B2 publication Critical patent/JP6930910B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/682Signal amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6846Common amplification features
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00Reactions characterised by their quantitative nature
    • C12Q2545/10Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/113Reactions characterised by their quantitative nature the purpose being quantitative analysis with an external standard/control, i.e. control reaction is separated from the test/target reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/166Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2017501008A 2014-07-09 2015-07-09 Rna転写産物バリアントを定量するための方法及び製品 Active JP6930910B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2021092115A JP7568581B2 (ja) 2014-07-09 2021-06-01 Rna転写産物バリアントを定量するための方法及び製品

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14176417.5 2014-07-09
EP14176417 2014-07-09
PCT/EP2015/065756 WO2016005524A1 (en) 2014-07-09 2015-07-09 Methods and products for quantifying rna transcript variants

Related Child Applications (2)

Application Number Title Priority Date Filing Date
JP2019182851A Division JP2020014478A (ja) 2014-07-09 2019-10-03 Rna転写産物バリアントを定量するための方法及び製品
JP2021092115A Division JP7568581B2 (ja) 2014-07-09 2021-06-01 Rna転写産物バリアントを定量するための方法及び製品

Publications (2)

Publication Number Publication Date
JP2017525341A JP2017525341A (ja) 2017-09-07
JP6930910B2 true JP6930910B2 (ja) 2021-09-01

Family

ID=51224706

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2017501008A Active JP6930910B2 (ja) 2014-07-09 2015-07-09 Rna転写産物バリアントを定量するための方法及び製品
JP2019182851A Pending JP2020014478A (ja) 2014-07-09 2019-10-03 Rna転写産物バリアントを定量するための方法及び製品
JP2021092115A Active JP7568581B2 (ja) 2014-07-09 2021-06-01 Rna転写産物バリアントを定量するための方法及び製品

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2019182851A Pending JP2020014478A (ja) 2014-07-09 2019-10-03 Rna転写産物バリアントを定量するための方法及び製品
JP2021092115A Active JP7568581B2 (ja) 2014-07-09 2021-06-01 Rna転写産物バリアントを定量するための方法及び製品

Country Status (10)

Country Link
US (1) US10513726B2 (enExample)
EP (1) EP3167076B1 (enExample)
JP (3) JP6930910B2 (enExample)
KR (1) KR102555447B1 (enExample)
CN (1) CN106471134B (enExample)
AU (1) AU2015286672B2 (enExample)
CA (1) CA2954495C (enExample)
DK (1) DK3167076T3 (enExample)
LT (1) LT3167076T (enExample)
WO (1) WO2016005524A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2012307282B2 (en) 2011-09-16 2018-03-15 Lexogen Gmbh Nucleic acid transcription method
AU2015286672B2 (en) 2014-07-09 2021-12-02 Lexogen Gmbh Methods and products for quantifying RNA transcript variants
CN106771194B (zh) * 2017-01-18 2018-07-20 青岛大学 一种检测病原微生物的方法
US10828330B2 (en) * 2017-02-22 2020-11-10 IO Bioscience, Inc. Nucleic acid constructs comprising gene editing multi-sites and uses thereof
CA3054307A1 (en) 2017-02-22 2018-08-30 Io Biosciences, Inc. Nucleic acid constructs comprising gene editing multi-sites and uses thereof
SE541799C2 (en) * 2018-04-11 2019-12-17 David Yudovich Determination of frequency distribution of nucleotide sequence variants
EP3781213A4 (en) 2018-04-17 2022-10-19 The Trustees of the University of Pennsylvania TRANS-SPLICE MOLECULES
CN116219037A (zh) * 2018-05-02 2023-06-06 宁夏农林科学院动物科学研究所 一种基于基因分型鉴定滩羊与非滩羊的方法
EP3666904A1 (en) 2018-12-14 2020-06-17 Lexogen GmbH Nucleic acid amplification and identification method
EP3901284A1 (en) * 2020-04-20 2021-10-27 BMBK Invest BV Construct, kit and method for sample tracking
KR102690574B1 (ko) 2020-06-26 2024-07-31 숙명여자대학교산학협력단 비타민 b1의 보호 및 전달을 위한 장용성 조성물
KR102690573B1 (ko) 2020-06-26 2024-07-31 숙명여자대학교산학협력단 사이클로덱스트린을 이용한 경구투여용 복합 조성물 및 이의 제조방법
WO2023086441A1 (en) * 2021-11-12 2023-05-19 Regents Of The University Of Minnesota Compositions and methods for transcriptional activation
EP4438741A4 (en) * 2021-11-26 2025-08-06 Daan Gene Co Ltd HIGH-THROUGHPUT SEQUENCING METHOD BASED ON THE INTERNAL REFERENCE OF A KNOWN MARKER
CA3252043A1 (en) * 2022-05-13 2023-11-16 Ascidian Therapeutics, Inc. ABCA4 TRANS-SPLICING MOLECULES

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2593343B1 (fr) 1986-01-20 1988-03-25 Thomson Csf Matrice d'elements photosensibles et son procede de fabrication, procede de lecture associe, et application de cette matrice a la prise de vue d'images
WO2002072889A2 (en) * 2001-01-12 2002-09-19 Applera Corporation Methods and compositions for microarray control
EP1386009A4 (en) 2001-05-07 2005-04-27 Amersham Biosciences Corp CONSTRUCTION OF ARTIFICIAL GENES FOR USE AS CONTROLS IN GENE EXPRESSION ANALYSIS SYSTEMS
US20040009512A1 (en) 2002-05-02 2004-01-15 Manuel Ares Arrays for detection of products of mRNA splicing
EP1730162A4 (en) * 2004-03-11 2008-12-10 Us Gov Health & Human Serv ARTIFICIAL MUTATIONAL CHECKS FOR DIAGNOSTIC TESTS
EP2333104A1 (en) * 2009-12-11 2011-06-15 Lexogen GmbH RNA analytics method
AU2015286672B2 (en) 2014-07-09 2021-12-02 Lexogen Gmbh Methods and products for quantifying RNA transcript variants

Also Published As

Publication number Publication date
KR20170028383A (ko) 2017-03-13
KR102555447B1 (ko) 2023-07-13
JP2017525341A (ja) 2017-09-07
DK3167076T3 (da) 2022-02-21
US10513726B2 (en) 2019-12-24
WO2016005524A1 (en) 2016-01-14
AU2015286672A1 (en) 2016-12-15
JP2020014478A (ja) 2020-01-30
JP2021153588A (ja) 2021-10-07
HK1231518A1 (zh) 2017-12-22
EP3167076A1 (en) 2017-05-17
JP7568581B2 (ja) 2024-10-16
CA2954495C (en) 2024-03-05
CN106471134A (zh) 2017-03-01
US20170321248A1 (en) 2017-11-09
CN106471134B (zh) 2021-11-12
LT3167076T (lt) 2022-02-10
EP3167076B1 (en) 2021-11-17
CA2954495A1 (en) 2016-01-14
AU2015286672B2 (en) 2021-12-02

Similar Documents

Publication Publication Date Title
JP7568581B2 (ja) Rna転写産物バリアントを定量するための方法及び製品
Chen et al. RNA editome in rhesus macaque shaped by purifying selection
Pfeiffer et al. Systematic evaluation of error rates and causes in short samples in next-generation sequencing
Hiller et al. Pre-mRNA secondary structures influence exon recognition
Kozarewa et al. Overview of target enrichment strategies
Lipson et al. Quantification of the yeast transcriptome by single-molecule sequencing
Gao et al. CIRI: an efficient and unbiased algorithm for de novo circular RNA identification
Li et al. Quality control of RNA-seq experiments
DK2245187T3 (en) Methods for accurate sequence data and modified due to localization
CN118638898A (zh) 用于靶向核酸序列富集的方法及在错误纠正的核酸测序中的应用
US20140221218A1 (en) Methods for single-molecule analysis
WO2012027446A2 (en) Nucleic acid sequence analysis
Wang et al. Forensic nanopore sequencing of microhaplotype markers using QitanTech’s QNome
Bedre et al. New era in plant alternative splicing analysis enabled by advances in high-throughput sequencing (HTS) technologies
JP5926189B2 (ja) Rna分析方法
WO2014128453A1 (en) Nucleic acid marker molecules for identifying and detecting cross contamination of nucleic acid samples
Zheng et al. Titration-free massively parallel pyrosequencing using trace amounts of starting material
US20210102246A1 (en) Genetic test for detecting congenital adrenal hyperplasia
Scheer et al. High-Resolution mapping of 3’extremities of RNA exosome substrates by 3’RACE-seq
Nikiforova et al. Amplification-based methods
Olsen et al. Transcriptomics: RNA-seq
Shin et al. Novel discovery of line-1 in a korean individual by a target enrichment method
Jain et al. Emerging Tools for Generating Genomics Data
Olsen et al. RNA-seq
Vecoli Next-generation sequencing technology in the genetics of cardiovascular disease

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20170707

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20180703

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20181002

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190104

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20190604

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20191003

C60 Trial request (containing other claim documents, opposition documents)

Free format text: JAPANESE INTERMEDIATE CODE: C60

Effective date: 20191003

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20191004

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20191024

C21 Notice of transfer of a case for reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C21

Effective date: 20191029

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20191227

C211 Notice of termination of reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C211

Effective date: 20200107

C22 Notice of designation (change) of administrative judge

Free format text: JAPANESE INTERMEDIATE CODE: C22

Effective date: 20200714

C13 Notice of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: C13

Effective date: 20201201

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210225

C22 Notice of designation (change) of administrative judge

Free format text: JAPANESE INTERMEDIATE CODE: C22

Effective date: 20210413

C302 Record of communication

Free format text: JAPANESE INTERMEDIATE CODE: C302

Effective date: 20210421

C302 Record of communication

Free format text: JAPANESE INTERMEDIATE CODE: C302

Effective date: 20210527

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210601

C23 Notice of termination of proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C23

Effective date: 20210608

C03 Trial/appeal decision taken

Free format text: JAPANESE INTERMEDIATE CODE: C03

Effective date: 20210713

C30A Notification sent

Free format text: JAPANESE INTERMEDIATE CODE: C3012

Effective date: 20210713

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20210812

R150 Certificate of patent or registration of utility model

Ref document number: 6930910

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250