CA2954495C - Methods and products for quantifying rna transcript variants - Google Patents

Methods and products for quantifying rna transcript variants Download PDF

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Publication number
CA2954495C
CA2954495C CA2954495A CA2954495A CA2954495C CA 2954495 C CA2954495 C CA 2954495C CA 2954495 A CA2954495 A CA 2954495A CA 2954495 A CA2954495 A CA 2954495A CA 2954495 C CA2954495 C CA 2954495C
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molecules
sequence
family
artificial
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French (fr)
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CA2954495A1 (en
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Lukas Paul
Petra KUBALA
Torsten Reda
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Lexogen GmbH
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Lexogen GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/113—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an external standard/control, i.e. control reaction is separated from the test/target reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2954495A 2014-07-09 2015-07-09 Methods and products for quantifying rna transcript variants Active CA2954495C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14176417.5 2014-07-09
EP14176417 2014-07-09
PCT/EP2015/065756 WO2016005524A1 (en) 2014-07-09 2015-07-09 Methods and products for quantifying rna transcript variants

Publications (2)

Publication Number Publication Date
CA2954495A1 CA2954495A1 (en) 2016-01-14
CA2954495C true CA2954495C (en) 2024-03-05

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Family Applications (1)

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CA2954495A Active CA2954495C (en) 2014-07-09 2015-07-09 Methods and products for quantifying rna transcript variants

Country Status (10)

Country Link
US (1) US10513726B2 (enExample)
EP (1) EP3167076B1 (enExample)
JP (3) JP6930910B2 (enExample)
KR (1) KR102555447B1 (enExample)
CN (1) CN106471134B (enExample)
AU (1) AU2015286672B2 (enExample)
CA (1) CA2954495C (enExample)
DK (1) DK3167076T3 (enExample)
LT (1) LT3167076T (enExample)
WO (1) WO2016005524A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013038010A2 (en) 2011-09-16 2013-03-21 Lexogen Gmbh Nucleic acid transcription method
LT3167076T (lt) 2014-07-09 2022-02-10 Lexogen Gmbh Metodai ir produktai, skirti rnr transkripto variantų kiekybiniam įvertinimui
CN106771194B (zh) * 2017-01-18 2018-07-20 青岛大学 一种检测病原微生物的方法
US10828330B2 (en) * 2017-02-22 2020-11-10 IO Bioscience, Inc. Nucleic acid constructs comprising gene editing multi-sites and uses thereof
US12440578B2 (en) 2017-02-22 2025-10-14 Io Biosciences, Inc. Nucleic acid constructs comprising gene editing multi-sites and uses thereof
SE541799C2 (en) * 2018-04-11 2019-12-17 David Yudovich Determination of frequency distribution of nucleotide sequence variants
KR20250150157A (ko) 2018-04-17 2025-10-17 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 트랜스-스플라이싱 분자
CN110438234B (zh) * 2018-05-02 2023-05-26 宁夏农林科学院动物科学研究所 一种基于基因分型鉴定滩羊与非滩羊的方法
EP3666904A1 (en) 2018-12-14 2020-06-17 Lexogen GmbH Nucleic acid amplification and identification method
EP3901284A1 (en) * 2020-04-20 2021-10-27 BMBK Invest BV Construct, kit and method for sample tracking
KR102690574B1 (ko) 2020-06-26 2024-07-31 숙명여자대학교산학협력단 비타민 b1의 보호 및 전달을 위한 장용성 조성물
WO2021261966A1 (ko) 2020-06-26 2021-12-30 숙명여자대학교 산학협력단 사이클로덱스트린을 이용한 경구투여용 복합 조성물 및 이의 제조방법
WO2023086441A1 (en) * 2021-11-12 2023-05-19 Regents Of The University Of Minnesota Compositions and methods for transcriptional activation
US20230167491A1 (en) * 2021-11-26 2023-06-01 Daan Gene Co., Ltd. Method for high-throughput sequencing based on internal reference with known index
KR20250011939A (ko) * 2022-05-13 2025-01-22 아시디엔 테라퓨틱스, 인크. Abca4 트랜스-스플라이싱 분자

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2593343B1 (fr) 1986-01-20 1988-03-25 Thomson Csf Matrice d'elements photosensibles et son procede de fabrication, procede de lecture associe, et application de cette matrice a la prise de vue d'images
WO2002072889A2 (en) * 2001-01-12 2002-09-19 Applera Corporation Methods and compositions for microarray control
JP2004532034A (ja) * 2001-05-07 2004-10-21 アマシャム・バイオサイエンス・コーポレイション 遺伝子発現分析システムでコントロールとして使用するための人工遺伝子のデザイン
US20040009512A1 (en) 2002-05-02 2004-01-15 Manuel Ares Arrays for detection of products of mRNA splicing
EP1730162A4 (en) * 2004-03-11 2008-12-10 Us Gov Health & Human Serv ARTIFICIAL MUTATIONAL CHECKS FOR DIAGNOSTIC TESTS
EP2333104A1 (en) * 2009-12-11 2011-06-15 Lexogen GmbH RNA analytics method
LT3167076T (lt) 2014-07-09 2022-02-10 Lexogen Gmbh Metodai ir produktai, skirti rnr transkripto variantų kiekybiniam įvertinimui

Also Published As

Publication number Publication date
WO2016005524A1 (en) 2016-01-14
EP3167076B1 (en) 2021-11-17
JP7568581B2 (ja) 2024-10-16
KR102555447B1 (ko) 2023-07-13
LT3167076T (lt) 2022-02-10
CN106471134A (zh) 2017-03-01
EP3167076A1 (en) 2017-05-17
CA2954495A1 (en) 2016-01-14
JP2021153588A (ja) 2021-10-07
HK1231518A1 (zh) 2017-12-22
DK3167076T3 (da) 2022-02-21
US20170321248A1 (en) 2017-11-09
CN106471134B (zh) 2021-11-12
AU2015286672A1 (en) 2016-12-15
AU2015286672B2 (en) 2021-12-02
US10513726B2 (en) 2019-12-24
JP2020014478A (ja) 2020-01-30
JP2017525341A (ja) 2017-09-07
JP6930910B2 (ja) 2021-09-01
KR20170028383A (ko) 2017-03-13

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