JP6916524B2 - 機能的タンパク質配列をディスプレイする生合成アミロイドに基づく材料 - Google Patents

機能的タンパク質配列をディスプレイする生合成アミロイドに基づく材料 Download PDF

Info

Publication number
JP6916524B2
JP6916524B2 JP2017552146A JP2017552146A JP6916524B2 JP 6916524 B2 JP6916524 B2 JP 6916524B2 JP 2017552146 A JP2017552146 A JP 2017552146A JP 2017552146 A JP2017552146 A JP 2017552146A JP 6916524 B2 JP6916524 B2 JP 6916524B2
Authority
JP
Japan
Prior art keywords
linker
polypeptide
csga
item
bacterial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2017552146A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018517398A (ja
JP2018517398A5 (https=
Inventor
ニール エス． ジョシ，
ニール エス． ジョシ，
ピーター キュー． グエン，
ピーター キュー． グエン，
アンナ ドゥラジ−タッテ，
アンナ ドゥラジ−タッテ，
ピチェト プラベスチョティノント，
ピチェト プラベスチョティノント，
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard University filed Critical Harvard University
Publication of JP2018517398A publication Critical patent/JP2018517398A/ja
Publication of JP2018517398A5 publication Critical patent/JP2018517398A5/ja
Priority to JP2021030187A priority Critical patent/JP7156726B2/ja
Application granted granted Critical
Publication of JP6916524B2 publication Critical patent/JP6916524B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicinal Preparation (AREA)
JP2017552146A 2015-04-06 2016-04-06 機能的タンパク質配列をディスプレイする生合成アミロイドに基づく材料 Expired - Fee Related JP6916524B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2021030187A JP7156726B2 (ja) 2015-04-06 2021-02-26 機能的タンパク質配列をディスプレイする生合成アミロイドに基づく材料

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562143560P 2015-04-06 2015-04-06
US62/143,560 2015-04-06
PCT/US2016/026161 WO2016164422A2 (en) 2015-04-06 2016-04-06 Biosynthetic amyloid-based materials displaying functional protein sequences

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2021030187A Division JP7156726B2 (ja) 2015-04-06 2021-02-26 機能的タンパク質配列をディスプレイする生合成アミロイドに基づく材料

Publications (3)

Publication Number Publication Date
JP2018517398A JP2018517398A (ja) 2018-07-05
JP2018517398A5 JP2018517398A5 (https=) 2019-05-16
JP6916524B2 true JP6916524B2 (ja) 2021-08-11

Family

ID=57072695

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2017552146A Expired - Fee Related JP6916524B2 (ja) 2015-04-06 2016-04-06 機能的タンパク質配列をディスプレイする生合成アミロイドに基づく材料
JP2021030187A Active JP7156726B2 (ja) 2015-04-06 2021-02-26 機能的タンパク質配列をディスプレイする生合成アミロイドに基づく材料

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2021030187A Active JP7156726B2 (ja) 2015-04-06 2021-02-26 機能的タンパク質配列をディスプレイする生合成アミロイドに基づく材料

Country Status (4)

Country Link
US (2) US20180258435A1 (https=)
EP (1) EP3280397A4 (https=)
JP (2) JP6916524B2 (https=)
WO (1) WO2016164422A2 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6618892B2 (ja) 2013-04-23 2019-12-11 プレジデント アンド フェローズ オブ ハーバード カレッジ 細菌バイオフィルムの遺伝的リプログラミング
EP3280397A4 (en) 2015-04-06 2018-08-22 President and Fellows of Harvard College Biosynthetic amyloid-based materials displaying functional protein sequences
WO2017201428A1 (en) * 2016-05-19 2017-11-23 President And Fellows Of Harvard College Methods of making gels and film using curli nanofibers
FR3099648B1 (fr) * 2019-07-30 2023-01-13 Centre Nat Rech Scient Membrane échangeuse d’ions
CN110585482B (zh) * 2019-08-02 2022-03-01 大连理工大学 一种具有抗菌性能的杂萘联苯聚芳醚腈及其表面改性方法
CN112608876B (zh) * 2020-12-30 2024-02-09 江苏大学 一种生物素化Curli蛋白的活细胞标记方法及其应用
GB202216905D0 (en) * 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
KR102855274B1 (ko) * 2022-12-06 2025-09-08 충남대학교산학협력단 strep-tag 결합물질에 대한 결합능을 가지는 융합단백질을 암호화하는 유전자 구조물 및 이를 함유하는 발현벡터, 재조합 균주 및 항암 조성물
CN120699871B (zh) * 2025-06-27 2026-03-06 吉林农业大学 一种黏膜修复型工程益生菌及其应用

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5762620A (en) 1992-04-02 1998-06-09 Ndm Acquisition Corp. Wound dressing containing a partially dehydrated hydrogel
US6864365B1 (en) * 1999-04-05 2005-03-08 Innovation And Development Corporation, University Of Victoria Bacterial fimbrial system for presentation of heterologous peptide sequences
DE60229867D1 (de) * 2001-07-02 2008-12-24 Merrion Res Iii Ltd Zielgerichtete ligande gegen peyer's flecke- und/oder m-zellen
CN101044154A (zh) * 2002-02-14 2007-09-26 威廉·J·鲁特 治疗宿主中切割的嵌合分子
EP1626799B1 (en) 2003-04-30 2010-04-14 Drexel University Thermogelling polymer blends for biomaterial applications
WO2006096515A2 (en) 2005-03-04 2006-09-14 Biorexis Pharmaceutical Corporation Modified transferrin fusion proteins
AU2008204442B2 (en) 2007-01-12 2013-03-14 Intrexon Actobiotics Nv Lactococcus promoters and uses thereof
JP2009073766A (ja) * 2007-09-21 2009-04-09 Otsuka Pharmaceut Co Ltd 医薬
WO2010111516A2 (en) 2009-03-25 2010-09-30 Whitehead Institute For Biomedical Research Compositions and methods for inhibiting biofilms
WO2010129033A2 (en) 2009-04-29 2010-11-11 Calmune Corporation Modified antibodies for passive immunotherapy
US20110108199A1 (en) 2009-11-10 2011-05-12 Tyco Healthcare Group Lp Hemostatic Tapes and Dispensers Therefor
JP2013519699A (ja) * 2010-02-16 2013-05-30 ノヴォ ノルディスク アー／エス 因子ｖｉｉｉ融合タンパク質
EP2552458A4 (en) 2010-03-31 2014-08-27 Univ Wayne State INJECTABLE DENDRIMER HYDROGEL NANOPARTICLES
KR20110111594A (ko) 2010-04-05 2011-10-12 서울대학교산학협력단 알파-시뉴클레인 유래 곱슬 아밀로이드 섬유의 제조방법, 이를 이용한 하이드로젤의 제조 방법 및 그 이용 방법
US8741417B2 (en) 2010-04-16 2014-06-03 Korea University Research And Business Foundation Films having switchable reflectivity
WO2012048289A1 (en) 2010-10-08 2012-04-12 Board Of Regents, The University Of Texas System Anti-adhesive barrier membrane using alginate and hyaluronic acid for biomedical applications
BR112013008957A2 (pt) * 2010-10-15 2019-09-24 Univ Cornell composições e métodos para tratamento de doenças endócrinas, gastrointestinais ou autoimunes
KR20120060442A (ko) 2010-12-02 2012-06-12 가톨릭대학교 산학협력단 위암 진단용 마커로서 ｔｆｆ2의 용도
US9140698B2 (en) * 2011-04-06 2015-09-22 The Board Of Regents Of The University Of Texas System Modulating bacterial MAM polypeptides in pathogenic disease
WO2012166906A1 (en) * 2011-05-31 2012-12-06 Massachusetts Institute Of Technology Cell-directed synthesis of multifunctional nanopatterns and nanomaterials
JP6006309B2 (ja) * 2011-07-08 2016-10-12 アミリン・ファーマシューティカルズ, リミテッド・ライアビリティ・カンパニーAmylin Pharmaceuticals, Llc 作用持続期間が増大し、免疫原性が減少した操作されたポリペプチド
CA2880791A1 (en) 2011-08-03 2013-02-07 Children's Medical Center Corporation A broadly neutralizing human antibody that recognizes the receptor-binding pocket of influenza hemagglutinin
WO2013188529A1 (en) 2012-06-15 2013-12-19 Temple University Of The Commonwealth System Of Higher Education Use of isolated bacterial amyloids for treatment of inflammatory disorders or diseases of the epithelium
IN2015DN01299A (https=) * 2012-07-23 2015-07-03 Zymeworks Inc
US20150291991A1 (en) 2012-11-16 2015-10-15 President And Fellows Of Harvard College Methods and compositions relating to amyloidogenic polypeptides
KR102211837B1 (ko) * 2013-01-14 2021-02-03 젠코어 인코포레이티드 신규한 이형이량체 단백질
JP2014156428A (ja) * 2013-02-15 2014-08-28 Univ Of Tokyo 抗体結合タンパク質
CN105518143B (zh) * 2013-03-15 2020-02-21 北京凯得尔森生物技术有限公司 融合蛋白分子和它的使用方法
JP6618892B2 (ja) 2013-04-23 2019-12-11 プレジデント アンド フェローズ オブ ハーバード カレッジ 細菌バイオフィルムの遺伝的リプログラミング
WO2015080671A1 (en) 2013-11-30 2015-06-04 Agency For Science, Technology And Research Self-assembling peptides, peptidomimetics and peptidic conjugates as building blocks for biofabrication and printing
WO2015097289A1 (en) 2013-12-24 2015-07-02 Vib Vzw Secretion and functional display of chimeric polypeptides
US20150342893A1 (en) 2014-05-29 2015-12-03 Sigmoid Pharma Limited Celecoxib formulations useful for treating colorectal cancer
EP3280397A4 (en) 2015-04-06 2018-08-22 President and Fellows of Harvard College Biosynthetic amyloid-based materials displaying functional protein sequences
WO2017201428A1 (en) 2016-05-19 2017-11-23 President And Fellows Of Harvard College Methods of making gels and film using curli nanofibers

Also Published As

Publication number Publication date
WO2016164422A3 (en) 2017-02-09
US20200248190A1 (en) 2020-08-06
EP3280397A2 (en) 2018-02-14
EP3280397A4 (en) 2018-08-22
JP7156726B2 (ja) 2022-10-19
JP2018517398A (ja) 2018-07-05
US11286491B2 (en) 2022-03-29
US20180258435A1 (en) 2018-09-13
JP2021097687A (ja) 2021-07-01
WO2016164422A2 (en) 2016-10-13

Similar Documents

Publication Publication Date Title
JP6916524B2 (ja) 機能的タンパク質配列をディスプレイする生合成アミロイドに基づく材料
US11352397B2 (en) Genetic reprogramming of bacterial biofilms
Marchesini et al. In search of Brucella abortus type IV secretion substrates: screening and identification of four proteins translocated into host cells through VirB system
Korotkov et al. Secretins: dynamic channels for protein transport across membranes
Johansson et al. Inhibition and dispersion of Pseudomonas aeruginosa biofilms by glycopeptide dendrimers targeting the fucose-specific lectin LecB
Allsopp et al. UpaH is a newly identified autotransporter protein that contributes to biofilm formation and bladder colonization by uropathogenic Escherichia coli CFT073
Waters et al. An amino‐terminal domain of Enterococcus faecalis aggregation substance is required for aggregation, bacterial internalization by epithelial cells and binding to lipoteichoic acid
Pandey et al. Co-regulation of iron metabolism and virulence associated functions by iron and XibR, a novel iron binding transcription factor, in the plant pathogen Xanthomonas
Wang et al. The adhesion of Lactobacillus salivarius REN to a human intestinal epithelial cell line requires s-layer proteins
Wei et al. Outer membrane vesicles secreted by Helicobacter pylori transmitting gastric pathogenic virulence factors
Wang et al. A type VI secretion system delivers a cell wall amidase to target bacterial competitors
US20160326220A1 (en) Secretion and functional display of chimeric polypeptides
Martinez-Gil et al. YeeJ is an inverse autotransporter from Escherichia coli that binds to peptidoglycan and promotes biofilm formation
US10745450B2 (en) Peptides and uses thereof
Wang et al. Rapid and efficient production of cecropin A antibacterial peptide in Escherichia coli by fusion with a self-aggregating protein
Gelfat et al. Single domain antibodies against enteric pathogen virulence factors are active as curli fiber fusions on probiotic E. coli Nissle 1917
JP2024508694A (ja) バクテリオファージリシン、そのキメラおよびその使用
Wong et al. The assembly of a hybrid type IV secretion system by a Crohn’s disease-associated Escherichia coli strain
Yi et al. Heterotrophic fermentation of a robust human defensin in Chlamydomonas reinhardtii provides a stable and potent antibacterial
Amro et al. TraC of the conjugative plasmid pKM101 is secreted as a monomer in outer membrane vesicles and stimulates conjugation
Estevens Collagen-like sequences as adhesion factors encoded by the pathogenic bacterium Burkholderia cenocepacia: computational analysis and experimental studies
Renn et al. Disulfide bond-mediated passenger domain stalling as a structural probe of autotransporter outer membrane secretion in vivo
Guo et al. Functionalizing Nisin with a Sugar Moiety Improves Its Solubility and Results in an Altered Antibacterial Spectrum and Mode of Action
Drummond Heterologous expression of the gcc gene cluster and subsequent characterisation of the glycocin F biosynthetic pathway: a thesis presented in partial fulfillment of the requirements for the degree of Master of Science in Biochemistry at Massey University, Manawatu, New Zealand
Oyama Prospecting Rumen Bacteria for Novel Antimicrobials

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20171018

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190405

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20190405

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20200127

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20200122

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20200413

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200727

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20201029

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210226

C60 Trial request (containing other claim documents, opposition documents)

Free format text: JAPANESE INTERMEDIATE CODE: C60

Effective date: 20210226

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20210308

C21 Notice of transfer of a case for reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C21

Effective date: 20210309

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20210412

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210527

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20210615

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20210709

R150 Certificate of patent or registration of utility model

Ref document number: 6916524

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees