JP6880001B2 - ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ - Google Patents

ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ Download PDF

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JP6880001B2
JP6880001B2 JP2018510803A JP2018510803A JP6880001B2 JP 6880001 B2 JP6880001 B2 JP 6880001B2 JP 2018510803 A JP2018510803 A JP 2018510803A JP 2018510803 A JP2018510803 A JP 2018510803A JP 6880001 B2 JP6880001 B2 JP 6880001B2
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target
hapten
antibody
enzyme
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JP2018528425A (ja
JP2018528425A5 (enExample
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ユリ ベロスルドツェフ,
ユリ ベロスルドツェフ,
トレイシー ディー. デギア,
トレイシー ディー. デギア,
ウェンディー ジェー. フレンチ,
ウェンディー ジェー. フレンチ,
チュンシャン ハオ,
チュンシャン ハオ,
ブライアン ダニエル ケリー,
ブライアン ダニエル ケリー,
アドリアン ムリーリョ,
アドリアン ムリーリョ,
ネイサン ウォルター ポラスケー,
ネイサン ウォルター ポラスケー,
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ヴェンタナ メディカル システムズ, インク.
ヴェンタナ メディカル システムズ, インク.
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07FACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02Phosphorus compounds
    • C07F9/547Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6558Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system
    • C07F9/65583Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system each of the hetero rings containing nitrogen as ring hetero atom
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

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  • Health & Medical Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
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  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
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  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2018510803A 2015-08-28 2016-08-26 ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ Active JP6880001B2 (ja)

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Application Number Priority Date Filing Date Title
US201562211590P 2015-08-28 2015-08-28
US62/211,590 2015-08-28
US201662301489P 2016-02-29 2016-02-29
US62/301,489 2016-02-29
PCT/US2016/049153 WO2017040349A1 (en) 2015-08-28 2016-08-26 Protein proximity assay in formalin fixed paffafin embedded tissue using caged haptens

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JP2018528425A JP2018528425A (ja) 2018-09-27
JP2018528425A5 JP2018528425A5 (enExample) 2019-10-03
JP6880001B2 true JP6880001B2 (ja) 2021-06-02

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JP2018510803A Active JP6880001B2 (ja) 2015-08-28 2016-08-26 ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ
JP2019511411A Active JP7033122B2 (ja) 2015-08-28 2017-08-25 ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ

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US (6) US11053266B2 (enExample)
EP (1) EP3341734B1 (enExample)
JP (2) JP6880001B2 (enExample)
CN (3) CN107923915B (enExample)
AU (3) AU2016316846B2 (enExample)
CA (1) CA2996798A1 (enExample)
WO (2) WO2017040349A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019532275A (ja) * 2015-08-28 2019-11-07 ヴェンタナ メディカル システムズ, インク. ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020121304A1 (en) * 2018-12-10 2020-06-18 Ariel Scientific Innovations Ltd. Therapeutic conjugates
CN110632040B (zh) * 2019-05-10 2022-03-29 长沙理工大学 一种血清中前列腺特异性抗原的分析方法
EP4022624A1 (en) * 2019-08-28 2022-07-06 Ventana Medical Systems, Inc. Assessing antigen retrieval and target retrieval progression quantitation with vibrational spectroscopy
JP2023524568A (ja) * 2020-05-07 2023-06-12 ヴェンタナ メディカル システムズ, インク. 腫瘍試料中のegfrおよびegfrリガンドの発現を評価するための組織化学的システムおよび方法
CN116783485A (zh) * 2021-01-15 2023-09-19 文塔纳医疗系统公司 储存稳定的笼状半抗原
CN114994329A (zh) * 2022-04-28 2022-09-02 杭州广科安德生物科技有限公司 一种检测肿瘤组织中p185蛋白与p95蛋白表达水平的方法

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2730523A (en) * 1952-08-16 1956-01-10 Eastman Kodak Co 5-nitrothiazoleazo-n-fluoroalkyl-aniline compounds
WO1990000618A1 (en) 1988-07-08 1990-01-25 Jbl Scientific, Inc. Preparation and use of fluorescent benzothiazole derivatives
US5182203A (en) * 1989-03-29 1993-01-26 E. I. Du Pont De Nemours And Company Bifunctional compounds useful in catalyzed reporter deposition
US5196306A (en) 1989-03-29 1993-03-23 E. I. Du Pont De Nemours And Company Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system
US5180828A (en) * 1990-02-09 1993-01-19 Molecular Devices Corporation Chromophoric reagents for incorporation of biotin or other haptens into macromolecules
US5595707A (en) 1990-03-02 1997-01-21 Ventana Medical Systems, Inc. Automated biological reaction apparatus
US5371241A (en) * 1991-07-19 1994-12-06 Pharmacia P-L Biochemicals Inc. Fluorescein labelled phosphoramidites
GB9517060D0 (en) * 1995-08-17 1995-10-25 Ciba Geigy Ag Acylated oligopeptide derivatives
US20030235877A1 (en) * 1996-04-30 2003-12-25 La Region Wallone Conjugates of haptens to beta-lactam derivatives and their use for detecting and/or quantifying haptens in solution and device for implementation thereof
EP1056541B1 (en) 1998-02-27 2017-10-25 Ventana Medical Systems, Inc. System and method of dispensing reagent
US6582962B1 (en) 1998-02-27 2003-06-24 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US20030211630A1 (en) 1998-02-27 2003-11-13 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US20060040319A1 (en) * 2000-01-14 2006-02-23 Tom Muir Multiple sensor-containing active modified polypeptides, preparation and uses thereof
JP2005502334A (ja) * 2001-08-07 2005-01-27 メルク エンド カムパニー インコーポレーテッド 生物学的アッセイ検出方法
CA2940803A1 (en) * 2004-03-23 2005-10-27 Ascendis Pharma Gmbh Prodrug linker
EP3144675A1 (en) 2005-04-28 2017-03-22 Ventana Medical Systems, Inc. Enzymes conjugated to antibodies via a peg heterobifuctional linker
CN101535244B (zh) 2005-11-23 2014-07-23 文塔納医疗系统公司 分子缀合物
ES2465465T3 (es) 2006-11-01 2014-06-05 Ventana Medical Systems, Inc. Haptenos, conjugados de haptenos, composiciones de los mismos y método para su preparación y uso
US20080220434A1 (en) * 2007-02-07 2008-09-11 Perscitus Biosciences, Llc Detection Of Molecule Proximity
EP3561513A1 (en) 2007-05-23 2019-10-30 Ventana Medical Systems, Inc. Polymeric carriers for immunohistochemistry and in situ hybridization
CN101952719A (zh) * 2007-07-27 2011-01-19 通信改革公司 检测测定法及其用途
CA2734783C (en) 2008-08-22 2016-04-19 Ventana Medical Systems, Inc. Method for chromogenic detection of two or more target molecules in a single sample
SG10201403755RA (en) 2009-10-19 2014-10-30 Ventana Med Syst Inc Imaging System And Techniques
EP2504684B1 (en) * 2009-11-27 2023-11-15 Ecole Polytechnique Federale De Lausanne (Epfl) Nanofluidic biosensor and its use for rapid measurement of biomolecular interactions in solution and methods
DK3088534T3 (en) 2010-12-30 2018-10-15 Ventana Med Syst Inc Improved deposition of chromogens using pyridine analogues
US8852599B2 (en) * 2011-05-26 2014-10-07 Bristol-Myers Squibb Company Immunoconjugates, compositions for making them, and methods of making and use
SG2014011084A (en) 2011-09-09 2014-04-28 Ventana Med Syst Inc Imaging systems, cassettes, and methods of using the same
WO2013148498A1 (en) 2012-03-27 2013-10-03 Ventana Medical Systems, Inc. Signaling conjugates and methods of use
AU2014230945B2 (en) * 2013-03-12 2019-07-11 Ventana Medical Systems, Inc. Proximity assay for in situ detection of targets
CN103513039B (zh) * 2013-07-10 2015-12-23 广州美格生物科技有限公司 一种利用定向肽库检测蛋白质与其他分子相互作用的方法
US10241115B2 (en) * 2013-12-10 2019-03-26 Merck Sharp & Dohme Corp. Immunohistochemical proximity assay for PD-1 positive cells and PD-ligand positive cells in tumor tissue
ES2713438T3 (es) * 2014-02-24 2019-05-21 Ventana Med Syst Inc Amplificación de señal de análogos de quinona metidas
US10550190B2 (en) 2014-04-04 2020-02-04 Merck Sharp & Dohme Corp. Phosphate based linkers for intracellular delivery of drug conjugates
CN107003323B (zh) * 2014-11-25 2019-06-04 文塔纳医疗系统公司 使用化学连接和半抗原转移的邻近测定法
EP3037820A1 (en) 2014-12-27 2016-06-29 Miltenyi Biotec GmbH Cell detection methods and reagents having a releasable labelling moiety
CN107923915B (zh) * 2015-08-28 2021-05-04 文塔纳医疗系统公司 使用经掩蔽的半抗原在福尔马林固定石蜡包埋的组织中的蛋白质邻近测定

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019532275A (ja) * 2015-08-28 2019-11-07 ヴェンタナ メディカル システムズ, インク. ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ
JP7033122B2 (ja) 2015-08-28 2022-03-09 ヴェンタナ メディカル システムズ, インク. ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ
US11345719B2 (en) 2015-08-28 2022-05-31 Vetana Medical Systems, Inc. Protein proximity assay in formalin fixed paraffin embedded tissue using caged haptens
US12173019B2 (en) 2015-08-28 2024-12-24 Ventana Medical Systems, Inc. Protein proximity assay in formalin fixed paffafin embedded tissue using caged haptens

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WO2017040349A1 (en) 2017-03-09
CN107923915A (zh) 2018-04-17
JP7033122B2 (ja) 2022-03-09
WO2018039605A1 (en) 2018-03-01
AU2016316846A1 (en) 2018-02-22
AU2016316846B2 (en) 2021-07-08
CN116338167A (zh) 2023-06-27
CN107923915B (zh) 2021-05-04
AU2021218128A1 (en) 2021-09-09
US12173019B2 (en) 2024-12-24
US20210309680A1 (en) 2021-10-07
JP2018528425A (ja) 2018-09-27
JP2019532275A (ja) 2019-11-07
EP3341734A4 (en) 2019-02-06
EP3341734A1 (en) 2018-07-04
EP3341734B1 (en) 2024-08-07
AU2023201682B2 (en) 2023-12-07
US20190233447A1 (en) 2019-08-01
US11345719B2 (en) 2022-05-31
US20220089622A1 (en) 2022-03-24
US11053266B2 (en) 2021-07-06
US20210221833A1 (en) 2021-07-22
AU2021218128B2 (en) 2022-12-22
AU2023201682A1 (en) 2023-04-13
CA2996798A1 (en) 2017-03-09
US20250066397A1 (en) 2025-02-27
CN109791144B (zh) 2023-04-18
CN109791144A (zh) 2019-05-21
US20180186821A1 (en) 2018-07-05

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