JP6880001B2 - ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ - Google Patents
ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ Download PDFInfo
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| CN110632040B (zh) * | 2019-05-10 | 2022-03-29 | 长沙理工大学 | 一种血清中前列腺特异性抗原的分析方法 |
| WO2021037869A1 (en) * | 2019-08-28 | 2021-03-04 | Ventana Medical Systems, Inc. | Assessing antigen retrieval and target retrieval progression quantitation with vibrational spectroscopy |
| CN115552248A (zh) * | 2020-05-07 | 2022-12-30 | 文塔纳医疗系统公司 | 用于评价肿瘤样品中egfr和egfr配体表达的组织化学系统和方法 |
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| US6582962B1 (en) | 1998-02-27 | 2003-06-24 | Ventana Medical Systems, Inc. | Automated molecular pathology apparatus having independent slide heaters |
| EP2284543A3 (en) | 1998-02-27 | 2015-05-27 | Ventana Medical Systems, Inc. | System and method of aspirating and dispensing reagent |
| US20060040319A1 (en) * | 2000-01-14 | 2006-02-23 | Tom Muir | Multiple sensor-containing active modified polypeptides, preparation and uses thereof |
| EP1442296A4 (en) * | 2001-08-07 | 2007-09-12 | Merck & Co Inc | BIOLOGICAL ASSAY DETECTION PROCEDURE |
| JP4950022B2 (ja) * | 2004-03-23 | 2012-06-13 | コンプレックス バイオシステムズ ゲーエムベーハー | 自壊性リンカーを有する高分子プロドラッグ |
| WO2006116628A2 (en) | 2005-04-28 | 2006-11-02 | Ventana Medical Systems, Inc. | Enzymes conjugated to antiobodies via a peg heterobifuctional linker |
| ES2548518T3 (es) | 2005-11-23 | 2015-10-19 | Ventana Medical Systems, Inc. | Conjugado molecular |
| ES2905054T3 (es) | 2006-11-01 | 2022-04-06 | Ventana Med Syst Inc | Haptenos, conjugados de haptenos, composiciones de los mismos y método para su preparación y uso |
| WO2008098100A2 (en) * | 2007-02-07 | 2008-08-14 | Perscitus Biosciences, Llc | Detection of molecule proximity |
| JP2010528285A (ja) | 2007-05-23 | 2010-08-19 | ベンタナ・メデイカル・システムズ・インコーポレーテツド | 免疫組織化学およびｉｎｓｉｔｕハイブリダーゼーションのためのポリマー担体 |
| WO2009018003A2 (en) * | 2007-07-27 | 2009-02-05 | Ensemble Discovery Corporation | Detection assays and use thereof |
| AU2009282760B2 (en) | 2008-08-22 | 2013-08-15 | Ventana Medical Systems, Inc. | Method for chromogenic detection of two or more target molecules in a single sample |
| JP5627698B2 (ja) | 2009-10-19 | 2014-11-19 | ベンタナ メディカル システムズ， インコーポレイテッド | 撮像システムおよび技法 |
| CA2782009C (en) * | 2009-11-27 | 2017-10-03 | Ecole Polytechnique Federale De Lausanne (Epfl) | Nanofluidic biosensor and its use for rapid measurement of biomolecular interactions in solution and methods |
| EP2659000B1 (en) | 2010-12-30 | 2016-08-10 | Ventana Medical Systems, Inc. | Enhanced deposition of chromogens utilizing pyrimidine analogs |
| US8852599B2 (en) * | 2011-05-26 | 2014-10-07 | Bristol-Myers Squibb Company | Immunoconjugates, compositions for making them, and methods of making and use |
| CA2928374C (en) | 2011-09-09 | 2018-09-25 | Ventana Medical Systems, Inc. | Imaging systems, cassettes, and methods of using the same |
| CA2867144C (en) | 2012-03-27 | 2019-07-02 | Ventana Medical Systems, Inc. | Signaling conjugates and methods of use |
| EP2971064B1 (en) * | 2013-03-12 | 2019-10-16 | Ventana Medical Systems, Inc. | Proximity assay for in situ detection of targets |
| CN103513039B (zh) * | 2013-07-10 | 2015-12-23 | 广州美格生物科技有限公司 | 一种利用定向肽库检测蛋白质与其他分子相互作用的方法 |
| US10241115B2 (en) * | 2013-12-10 | 2019-03-26 | Merck Sharp & Dohme Corp. | Immunohistochemical proximity assay for PD-1 positive cells and PD-ligand positive cells in tumor tissue |
| WO2015124703A1 (en) * | 2014-02-24 | 2015-08-27 | Ventana Medical Systems, Inc. | Quinone methide analog signal amplification |
| WO2015153401A1 (en) * | 2014-04-04 | 2015-10-08 | Merck Sharp & Dohme Corp | Phosphate based linkers for intracellular delivery of drug conjugates |
| EP3224621B1 (en) * | 2014-11-25 | 2019-06-12 | Ventana Medical Systems, Inc. | Proximity assays using chemical ligation and hapten transfer |
| EP3037820A1 (en) * | 2014-12-27 | 2016-06-29 | Miltenyi Biotec GmbH | Cell detection methods and reagents having a releasable labelling moiety |
| CN107923915B (zh) * | 2015-08-28 | 2021-05-04 | 文塔纳医疗系统公司 | 使用经掩蔽的半抗原在福尔马林固定石蜡包埋的组织中的蛋白质邻近测定 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019532275A (ja) * | 2015-08-28 | 2019-11-07 | ヴェンタナ メディカル システムズ， インク． | ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ |
| JP7033122B2 (ja) | 2015-08-28 | 2022-03-09 | ヴェンタナ メディカル システムズ， インク． | ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ |
| US11345719B2 (en) | 2015-08-28 | 2022-05-31 | Vetana Medical Systems, Inc. | Protein proximity assay in formalin fixed paraffin embedded tissue using caged haptens |
| US12173019B2 (en) | 2015-08-28 | 2024-12-24 | Ventana Medical Systems, Inc. | Protein proximity assay in formalin fixed paffafin embedded tissue using caged haptens |
| US12559511B2 (en) | 2015-08-28 | 2026-02-24 | Ventana Medical Systems, Inc. | Protein proximity assay in formalin fixed paraffin embedded tissue using caged haptens |
| US12577266B2 (en) | 2015-08-28 | 2026-03-17 | Ventana Medical Systems, Inc. | Protein proximity assay in formalin fixed paraffin embedded tissue using caged haptens |
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