JP6871168B2 - 方法 - Google Patents

方法 Download PDF

Info

Publication number
JP6871168B2
JP6871168B2 JP2017546051A JP2017546051A JP6871168B2 JP 6871168 B2 JP6871168 B2 JP 6871168B2 JP 2017546051 A JP2017546051 A JP 2017546051A JP 2017546051 A JP2017546051 A JP 2017546051A JP 6871168 B2 JP6871168 B2 JP 6871168B2
Authority
JP
Japan
Prior art keywords
protein
binder
library
binding
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017546051A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017536846A5 (cg-RX-API-DMAC7.html
JP2017536846A (ja
Inventor
ジェニー,チャバ
Original Assignee
ジーンビレッジ コルラートルト フェレレーシェシェーギュー タールシャシャーグ
ジーンビレッジ コルラートルト フェレレーシェシェーギュー タールシャシャーグ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ジーンビレッジ コルラートルト フェレレーシェシェーギュー タールシャシャーグ, ジーンビレッジ コルラートルト フェレレーシェシェーギュー タールシャシャーグ filed Critical ジーンビレッジ コルラートルト フェレレーシェシェーギュー タールシャシャーグ
Publication of JP2017536846A publication Critical patent/JP2017536846A/ja
Publication of JP2017536846A5 publication Critical patent/JP2017536846A5/ja
Application granted granted Critical
Publication of JP6871168B2 publication Critical patent/JP6871168B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845Methods of identifying protein-protein interactions in protein mixtures
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00Methods of screening libraries
    • C40B30/04Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1037Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00Labels used in chemical analysis of biological material
    • G01N2458/10Oligonucleotides as tagging agents for labelling antibodies
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00Screening for compounds of potential therapeutic value
    • G01N2500/04Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2017546051A 2014-11-24 2015-11-24 方法 Active JP6871168B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1420852.4 2014-11-24
GBGB1420852.4A GB201420852D0 (en) 2014-11-24 2014-11-24 Method
PCT/GB2015/053573 WO2016083793A1 (en) 2014-11-24 2015-11-24 Method

Publications (3)

Publication Number Publication Date
JP2017536846A JP2017536846A (ja) 2017-12-14
JP2017536846A5 JP2017536846A5 (cg-RX-API-DMAC7.html) 2018-12-27
JP6871168B2 true JP6871168B2 (ja) 2021-05-12

Family

ID=52292436

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017546051A Active JP6871168B2 (ja) 2014-11-24 2015-11-24 方法

Country Status (10)

Country Link
US (3) US10605814B2 (cg-RX-API-DMAC7.html)
EP (1) EP3224360B1 (cg-RX-API-DMAC7.html)
JP (1) JP6871168B2 (cg-RX-API-DMAC7.html)
KR (1) KR102422494B1 (cg-RX-API-DMAC7.html)
CN (1) CN107430130B (cg-RX-API-DMAC7.html)
CA (1) CA2968488C (cg-RX-API-DMAC7.html)
ES (1) ES2763563T3 (cg-RX-API-DMAC7.html)
GB (1) GB201420852D0 (cg-RX-API-DMAC7.html)
SG (2) SG11201703689SA (cg-RX-API-DMAC7.html)
WO (1) WO2016083793A1 (cg-RX-API-DMAC7.html)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017214211A1 (en) * 2016-06-09 2017-12-14 Igc Bio, Inc. Methods for identifying a high affinity antibody
US20190352636A1 (en) * 2017-01-31 2019-11-21 The Governors Of The University Of Alberta Display of molecules on silently genetically encoded nanoscale carriers for determining synergistic molecular interactions
WO2019085958A1 (zh) * 2017-11-06 2019-05-09 清华大学 检测生物分子间相互作用及其调控因子的成套试剂与应用
SG11202100721SA (en) 2018-07-23 2021-02-25 Univ Alberta Genetically-encoded bicyclic peptide libraries
ES2964575T3 (es) 2019-06-25 2024-04-08 Actome Gmbh Método y kit para la medición de analitos en sistemas bicomponentes y sus usos
JP7727628B2 (ja) 2019-12-03 2025-08-21 アラマー バイオサイエンシーズ, インク. 核酸結合免疫サンドイッチアッセイ(nulisa)
US20240209350A1 (en) * 2021-04-26 2024-06-27 European Molecular Biology Laboratory High complexity microcompartment-based interaction screening
EP4242656A1 (en) * 2022-03-10 2023-09-13 Actome GmbH Quantitative detection method and corresponding device
CA3260523A1 (en) 2022-06-29 2024-01-04 Actome Gmbh DETECTION OF BIOMOLECULES IN SINGLE CELLS
GB202218453D0 (en) 2022-12-08 2023-01-25 Actome Gmbh Assay system

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
ATE199392T1 (de) 1992-12-04 2001-03-15 Medical Res Council Multivalente und multispezifische bindungsproteine, deren herstellung und verwendung
US7306904B2 (en) 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
GB0422431D0 (en) * 2004-10-08 2004-11-10 Affitech As Method
CN101896605A (zh) * 2007-10-12 2010-11-24 普罗诺塔股份有限公司 适体在蛋白质组学中的用途
GB0903316D0 (en) * 2009-02-26 2009-04-08 Affitech As Method
WO2010135558A1 (en) 2009-05-20 2010-11-25 Novimmune S.A. Systhetic polypeptide libraries and methods for generating naturally diversified polypeptide variants
SG177395A1 (en) * 2009-07-07 2012-02-28 Agency Science Tech & Res Methods of identifying a pair of binding partners
DK2622073T5 (en) * 2010-09-27 2019-02-25 Vipergen Method of Preparing an Enriched Library
US9209965B2 (en) 2014-01-14 2015-12-08 Microsemi Semiconductor Ulc Network interface with clock recovery module on line card

Also Published As

Publication number Publication date
SG10202008300RA (en) 2020-10-29
KR20170086627A (ko) 2017-07-26
EP3224360B1 (en) 2019-10-09
GB201420852D0 (en) 2015-01-07
JP2017536846A (ja) 2017-12-14
US11421347B2 (en) 2022-08-23
WO2016083793A1 (en) 2016-06-02
US20170269098A1 (en) 2017-09-21
US20210003585A1 (en) 2021-01-07
US10605814B2 (en) 2020-03-31
CA2968488C (en) 2024-09-10
US20230175171A1 (en) 2023-06-08
EP3224360A1 (en) 2017-10-04
CN107430130B (zh) 2020-07-24
CA2968488A1 (en) 2016-06-02
KR102422494B1 (ko) 2022-07-19
SG11201703689SA (en) 2017-06-29
ES2763563T3 (es) 2020-05-29
CN107430130A (zh) 2017-12-01

Similar Documents

Publication Publication Date Title
JP6871168B2 (ja) 方法
CN103134938B (zh) 适体在蛋白质组学中的用途
JP2022513092A (ja) 親和性試薬の設計および選択
JP2017525390A (ja) 組換えタンパク質調製物中の残留宿主細胞タンパク質の検出
CN102449476A (zh) 筛选抑制目标蛋白质-蛋白质相互作用的候选新药以开发一类药物的方法
Paduch et al. Generating conformation and complex-specific synthetic antibodies
US10191049B2 (en) Screening methods and uses thereof
JP4700626B2 (ja) 分子上のエピトープの免疫検出のための試薬、キット及び方法
Ch’ng et al. Phage Display-Derived Antibodies: Application of Recombinant Antibodies for Diagnostics./Ed
Jara-Acevedo et al. Screening phage-display antibody libraries using protein arrays
Konthur et al. High-throughput applications of phage display in proteomic analyses
JP2025524400A (ja) 抗リガンドを単離する方法
US20240117526A1 (en) Methods and compositions for protein detection
Song et al. Peptide Aptamer Microarrays: Bridging the bio–detector interface
CA3236602A1 (en) Methods and compositions for protein detection
JP2018027024A (ja) アプタマー及び抗体検出方法
US20180119202A1 (en) Proximity-enhanced nucleic acid-amplified protein detection
Poolsup Discovery of DNA Aptamers Targeting SARS-CoV-2 Proteins and Protein Binding Epitopes Identification for Label-Free COVID-19 Diagnostics
WO2025262670A1 (en) Simultaneous detection of proteins and nucleic acids using proximity ligation of aptamers and quantitative polymerase chain reaction
WO2023070034A1 (en) High sensitivity dna linked immunosorbent signal amplification assay (dlisa) for detection of infectious sars-cov-2 virus and variants
Saragozza High-throughput antibody validation platforms
HK1091540B (en) Use of a virus expressing a binding moiety to measure analytes in a sample
HK1182745B (en) Methods and systems for generating, validating and using monoclonal antibodies
HK1091540A1 (zh) 使用表达结合部分的病毒测量样品中的分析物
HK1182745A1 (zh) 用於產生、確認和使用單克隆抗體的方法和系統

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170719

A529 Written submission of copy of amendment under article 34 pct

Free format text: JAPANESE INTERMEDIATE CODE: A529

Effective date: 20170721

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20181116

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20181116

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20191001

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20190927

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20191128

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200228

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20200804

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20201001

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20210323

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20210415

R150 Certificate of patent or registration of utility model

Ref document number: 6871168

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250