JP6824881B2 - ナノ細孔rnaを特徴付けるための方法 - Google Patents

ナノ細孔rnaを特徴付けるための方法 Download PDF

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JP6824881B2
JP6824881B2 JP2017520492A JP2017520492A JP6824881B2 JP 6824881 B2 JP6824881 B2 JP 6824881B2 JP 2017520492 A JP2017520492 A JP 2017520492A JP 2017520492 A JP2017520492 A JP 2017520492A JP 6824881 B2 JP6824881 B2 JP 6824881B2
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rna
dna
polynucleotide
seq
rna polynucleotide
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JP2017532962A (ja
JP2017532962A5 (OSRAM
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ライアン ガラルド,ダニエル
ライアン ガラルド,ダニエル
ジョン ヘロン,アンドリュー
ジョン ヘロン,アンドリュー
ジャヤシンゲ,ラクマル
ジョン ターナー,ダニエル
ジョン ターナー,ダニエル
ホワイト,ジェームズ
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オックスフォード ナノポール テクノロジーズ リミテッド
オックスフォード ナノポール テクノロジーズ リミテッド
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Priority claimed from GBGB1508270.4A external-priority patent/GB201508270D0/en
Priority claimed from PCT/GB2015/052916 external-priority patent/WO2016055777A2/en
Priority claimed from GBGB1517634.0A external-priority patent/GB201517634D0/en
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12YENZYMES
    • C12Y306/00Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/04Hydrolases acting on acid anhydrides (3.6) acting on acid anhydrides; involved in cellular and subcellular movement (3.6.4)
    • C12Y306/04012DNA helicase (3.6.4.12)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44756Apparatus specially adapted therefor
    • G01N27/44791Microapparatus
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/48707Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721Investigating individual macromolecules, e.g. by translocation through nanopores
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    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/50Other enzymatic activities
    • C12Q2521/513Winding/unwinding enzyme, e.g. helicase
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    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/121Modifications characterised by incorporating both deoxyribonucleotides and ribonucleotides
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    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/197Modifications characterised by incorporating a spacer/coupling moiety
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    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60Detection means characterised by use of a special device
    • C12Q2565/631Detection means characterised by use of a special device being a biochannel or pore

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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JP2017520492A 2014-10-17 2015-10-19 ナノ細孔rnaを特徴付けるための方法 Active JP6824881B2 (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
GB1418459.2 2014-10-17
GB201418459A GB201418459D0 (en) 2014-10-17 2014-10-17 Method
GB1508270.4 2015-05-14
GBGB1508270.4A GB201508270D0 (en) 2015-05-14 2015-05-14 Method
GBPCT/GB2015/052916 2015-10-06
GB1517634.0 2015-10-06
PCT/GB2015/052916 WO2016055777A2 (en) 2014-10-07 2015-10-06 Modified enzymes
GBGB1517634.0A GB201517634D0 (en) 2015-10-06 2015-10-06 Method
PCT/GB2015/053097 WO2016059436A1 (en) 2014-10-17 2015-10-19 Method for nanopore rna characterisation

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JP2017532962A JP2017532962A (ja) 2017-11-09
JP2017532962A5 JP2017532962A5 (OSRAM) 2018-11-29
JP6824881B2 true JP6824881B2 (ja) 2021-02-03

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US (4) US10480026B2 (OSRAM)
EP (1) EP3207155B1 (OSRAM)
JP (1) JP6824881B2 (OSRAM)
KR (1) KR102457147B1 (OSRAM)
CN (2) CN107109489B (OSRAM)
WO (1) WO2016059436A1 (OSRAM)

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US11021747B2 (en) 2021-06-01
US20230012471A9 (en) 2023-01-12
EP3207155A1 (en) 2017-08-23
JP2017532962A (ja) 2017-11-09
CN107109489A (zh) 2017-08-29
CN113981055A (zh) 2022-01-28
EP3207155B1 (en) 2019-06-05
CN107109489B (zh) 2021-11-30
US12129518B2 (en) 2024-10-29
US20250084472A1 (en) 2025-03-13
US20210395811A1 (en) 2021-12-23
US20200063199A1 (en) 2020-02-27
KR102457147B1 (ko) 2022-10-19
US20170253923A1 (en) 2017-09-07

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