KR102457147B1 - 나노 세공 rna 특징 규명을 위한 방법 - Google Patents
나노 세공 rna 특징 규명을 위한 방법 Download PDFInfo
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- KR102457147B1 KR102457147B1 KR1020177013136A KR20177013136A KR102457147B1 KR 102457147 B1 KR102457147 B1 KR 102457147B1 KR 1020177013136 A KR1020177013136 A KR 1020177013136A KR 20177013136 A KR20177013136 A KR 20177013136A KR 102457147 B1 KR102457147 B1 KR 102457147B1
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- rna
- dna
- polynucleotide
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- rna polynucleotide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
- C12Y306/04—Hydrolases acting on acid anhydrides (3.6) acting on acid anhydrides; involved in cellular and subcellular movement (3.6.4)
- C12Y306/04012—DNA helicase (3.6.4.12)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44791—Microapparatus
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/513—Winding/unwinding enzyme, e.g. helicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/121—Modifications characterised by incorporating both deoxyribonucleotides and ribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/197—Modifications characterised by incorporating a spacer/coupling moiety
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Nanotechnology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Hematology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Electrochemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1418459.2 | 2014-10-17 | ||
| GB201418459A GB201418459D0 (en) | 2014-10-17 | 2014-10-17 | Method |
| GB1508270.4 | 2015-05-14 | ||
| GBGB1508270.4A GB201508270D0 (en) | 2015-05-14 | 2015-05-14 | Method |
| GBPCT/GB2015/052916 | 2015-10-06 | ||
| GB1517634.0 | 2015-10-06 | ||
| PCT/GB2015/052916 WO2016055777A2 (en) | 2014-10-07 | 2015-10-06 | Modified enzymes |
| GBGB1517634.0A GB201517634D0 (en) | 2015-10-06 | 2015-10-06 | Method |
| PCT/GB2015/053097 WO2016059436A1 (en) | 2014-10-17 | 2015-10-19 | Method for nanopore rna characterisation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20170072915A KR20170072915A (ko) | 2017-06-27 |
| KR102457147B1 true KR102457147B1 (ko) | 2022-10-19 |
Family
ID=54337815
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020177013136A Active KR102457147B1 (ko) | 2014-10-17 | 2015-10-19 | 나노 세공 rna 특징 규명을 위한 방법 |
Country Status (6)
| Country | Link |
|---|---|
| US (4) | US10480026B2 (OSRAM) |
| EP (1) | EP3207155B1 (OSRAM) |
| JP (1) | JP6824881B2 (OSRAM) |
| KR (1) | KR102457147B1 (OSRAM) |
| CN (2) | CN107109489B (OSRAM) |
| WO (1) | WO2016059436A1 (OSRAM) |
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| US9758823B2 (en) | 2011-10-21 | 2017-09-12 | Oxford Nanopore Technologies Limited | Enzyme method |
| IN2014DN06796A (OSRAM) | 2012-02-15 | 2015-05-22 | Oxford Nanopore Tech Ltd | |
| WO2013153359A1 (en) | 2012-04-10 | 2013-10-17 | Oxford Nanopore Technologies Limited | Mutant lysenin pores |
| WO2013185137A1 (en) | 2012-06-08 | 2013-12-12 | Pacific Biosciences Of California, Inc. | Modified base detection with nanopore sequencing |
| WO2014013260A1 (en) | 2012-07-19 | 2014-01-23 | Oxford Nanopore Technologies Limited | Modified helicases |
| GB201318465D0 (en) | 2013-10-18 | 2013-12-04 | Oxford Nanopore Tech Ltd | Method |
| CN117947149A (zh) | 2013-10-18 | 2024-04-30 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| GB201417712D0 (en) * | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| EP4053150A1 (en) | 2014-09-01 | 2022-09-07 | Vib Vzw | Mutant csgg pores |
| US10480026B2 (en) | 2014-10-17 | 2019-11-19 | Oxford Nanopore Technologies Ltd. | Method for nanopore RNA characterisation |
| EP3423486B1 (en) | 2016-03-02 | 2021-12-29 | Oxford Nanopore Technologies PLC | Mutant pore |
| US11514289B1 (en) | 2016-03-09 | 2022-11-29 | Freenome Holdings, Inc. | Generating machine learning models using genetic data |
| CA3020203A1 (en) | 2016-04-06 | 2017-10-12 | Oxford Nanopore Technologies Limited | Mutant pore |
| GB201609221D0 (en) * | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| WO2017203268A1 (en) * | 2016-05-25 | 2017-11-30 | Oxford Nanopore Technologies Limited | Method |
| GB201617886D0 (en) * | 2016-10-21 | 2016-12-07 | Oxford Nanopore Technologies Limited | Method |
| GB201620450D0 (en) * | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| US11578362B2 (en) * | 2017-04-27 | 2023-02-14 | The Regents Of The University Of California | Methods of producing ribosomal ribonucleic acid complexes |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| JP7362112B2 (ja) * | 2017-06-29 | 2023-10-17 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ナノポアを通るポリマーの決定論的ステップ |
| CN117106037B (zh) | 2017-06-30 | 2025-07-25 | 弗拉芒区生物技术研究所 | 新颖蛋白孔 |
| GB2569977A (en) * | 2018-01-05 | 2019-07-10 | Oxford Nanopore Tech Ltd | Method |
| WO2019149626A1 (de) | 2018-02-02 | 2019-08-08 | Bayer Aktiengesellschaft | Bekämpfung resistenter schadorganismen |
| WO2019226822A1 (en) * | 2018-05-23 | 2019-11-28 | The Regents Of The University Of California | Methods of analyzing capped ribonucleic acids |
| GB201809323D0 (en) | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
| EP3650558A1 (en) * | 2018-11-07 | 2020-05-13 | Siemens Healthcare GmbH | Liquid sample workflow for nanopore sequencing |
| EP3650559B1 (en) | 2018-11-08 | 2022-06-29 | Siemens Healthcare GmbH | Direct rna nanopore sequencing with help of a stem-loop polynucleotide |
| CN111455031A (zh) * | 2019-01-18 | 2020-07-28 | 中国科学院微生物研究所 | 基于Nanopore测序技术的多组学测序及分析方法 |
| CN110093399A (zh) * | 2019-04-22 | 2019-08-06 | 武汉大学 | 一种利用二磷酸尿嘧啶脱氧核苷酸检测核酸中腺嘌呤n6位发生甲基化修饰的方法 |
| GB201907244D0 (en) * | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| US11926819B2 (en) | 2019-05-28 | 2024-03-12 | The Regents Of The University Of California | Methods of adding polymers to ribonucleic acids |
| WO2021036995A1 (en) * | 2019-08-23 | 2021-03-04 | Nanjing University | Direct microrna sequencing using enzyme assisted sequencing |
| CN110568039B (zh) * | 2019-09-06 | 2021-05-04 | 中国科学院生物物理研究所 | 使用新型电化学传感器的氨基酸特异实时检测方法 |
| CN111164097B (zh) * | 2019-09-29 | 2021-02-19 | 北京齐碳科技有限公司 | 一种Mnep单体变体及其应用 |
| CN113930406B (zh) * | 2021-12-17 | 2022-04-08 | 北京齐碳科技有限公司 | 一种Pif1-like解旋酶及其应用 |
| GB202107192D0 (en) * | 2021-05-19 | 2021-06-30 | Oxford Nanopore Tech Ltd | Method |
| CN114134142B (zh) * | 2021-11-23 | 2024-05-28 | 成都齐碳科技有限公司 | 衔接体、复合物、单链分子、试剂盒、方法和应用 |
| WO2023116575A1 (zh) * | 2021-12-21 | 2023-06-29 | 成都齐碳科技有限公司 | 用于表征目标多核苷酸的衔接体、方法及其用途 |
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| CN119213145A (zh) * | 2022-05-27 | 2024-12-27 | 深圳华大生命科学研究院 | 一种维持纳米孔测序速度的方法 |
| CN120457488A (zh) | 2022-11-10 | 2025-08-08 | 拜耳公司 | 基于遗传谱的靶向性作物保护产品施用 |
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| CN104136631B (zh) | 2011-12-29 | 2017-03-01 | 牛津纳米孔技术公司 | 使用xpd解旋酶表征多核苷酸的方法 |
| IN2014DN06796A (OSRAM) | 2012-02-15 | 2015-05-22 | Oxford Nanopore Tech Ltd | |
| BR102012007246A2 (pt) | 2012-03-30 | 2016-02-10 | Fundação Antonio Prudente | métodos para prognóstico e classificação de resultados de um evento |
| WO2013153359A1 (en) | 2012-04-10 | 2013-10-17 | Oxford Nanopore Technologies Limited | Mutant lysenin pores |
| AU2013291765C1 (en) | 2012-07-19 | 2019-08-08 | Oxford Nanopore Technologies Limited | Enzyme construct |
| EP2875154B1 (en) | 2012-07-19 | 2017-08-23 | Oxford Nanopore Technologies Limited | SSB method for characterising a nucleic acid |
| WO2014013260A1 (en) * | 2012-07-19 | 2014-01-23 | Oxford Nanopore Technologies Limited | Modified helicases |
| EP2895618B1 (en) | 2012-09-14 | 2017-07-26 | Oxford Nanopore Technologies Limited | Sample preparation method |
| EP2917366B1 (en) | 2012-11-06 | 2017-08-02 | Oxford Nanopore Technologies Limited | Quadruplex method |
| GB201318465D0 (en) * | 2013-10-18 | 2013-12-04 | Oxford Nanopore Tech Ltd | Method |
| KR102168813B1 (ko) | 2013-03-08 | 2020-10-22 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 효소 정지 방법 |
| CN117947149A (zh) | 2013-10-18 | 2024-04-30 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| GB201406151D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| EP3126515B1 (en) | 2014-04-04 | 2018-08-29 | Oxford Nanopore Technologies Limited | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
| US10480026B2 (en) | 2014-10-17 | 2019-11-19 | Oxford Nanopore Technologies Ltd. | Method for nanopore RNA characterisation |
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2015
- 2015-10-19 US US15/519,599 patent/US10480026B2/en active Active
- 2015-10-19 EP EP15784137.0A patent/EP3207155B1/en active Active
- 2015-10-19 WO PCT/GB2015/053097 patent/WO2016059436A1/en not_active Ceased
- 2015-10-19 JP JP2017520492A patent/JP6824881B2/ja active Active
- 2015-10-19 CN CN201580069027.9A patent/CN107109489B/zh active Active
- 2015-10-19 KR KR1020177013136A patent/KR102457147B1/ko active Active
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|---|---|
| US10480026B2 (en) | 2019-11-19 |
| KR20170072915A (ko) | 2017-06-27 |
| JP6824881B2 (ja) | 2021-02-03 |
| WO2016059436A1 (en) | 2016-04-21 |
| US11021747B2 (en) | 2021-06-01 |
| US20230012471A9 (en) | 2023-01-12 |
| EP3207155A1 (en) | 2017-08-23 |
| JP2017532962A (ja) | 2017-11-09 |
| CN107109489A (zh) | 2017-08-29 |
| CN113981055A (zh) | 2022-01-28 |
| EP3207155B1 (en) | 2019-06-05 |
| CN107109489B (zh) | 2021-11-30 |
| US12129518B2 (en) | 2024-10-29 |
| US20250084472A1 (en) | 2025-03-13 |
| US20210395811A1 (en) | 2021-12-23 |
| US20200063199A1 (en) | 2020-02-27 |
| US20170253923A1 (en) | 2017-09-07 |
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