JP6804467B2 - 組換え型ヌクレオシド特異的リボヌクレアーゼ及びその生成法と使用法 - Google Patents

組換え型ヌクレオシド特異的リボヌクレアーゼ及びその生成法と使用法 Download PDF

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JP6804467B2
JP6804467B2 JP2017555346A JP2017555346A JP6804467B2 JP 6804467 B2 JP6804467 B2 JP 6804467B2 JP 2017555346 A JP2017555346 A JP 2017555346A JP 2017555346 A JP2017555346 A JP 2017555346A JP 6804467 B2 JP6804467 B2 JP 6804467B2
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protein
recombinant
rnase
rna
kusatibin
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JP2018512883A (ja
JP2018512883A5 (https=
Inventor
バラスブラマニヤム・アッデパッリ
ニコラス・ポール・レスナー
パトリック・アラン・リンバック
サラ・ヴィーナス
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Cincinnati Childrens Hospital Medical Center
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Cincinnati Childrens Hospital Medical Center
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2017555346A 2015-04-23 2016-04-25 組換え型ヌクレオシド特異的リボヌクレアーゼ及びその生成法と使用法 Active JP6804467B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562151640P 2015-04-23 2015-04-23
US201562151546P 2015-04-23 2015-04-23
US62/151,640 2015-04-23
US62/151,546 2015-04-23
PCT/US2016/029151 WO2016172678A2 (en) 2015-04-23 2016-04-25 Recombinant nucleotide-specific ribonuclease and method of producing and using same

Related Child Applications (1)

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JP2020163486A Division JP2021006049A (ja) 2015-04-23 2020-09-29 組換え型ヌクレオシド特異的リボヌクレアーゼ及びその生成法と使用法

Publications (3)

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JP2018512883A JP2018512883A (ja) 2018-05-24
JP2018512883A5 JP2018512883A5 (https=) 2019-10-17
JP6804467B2 true JP6804467B2 (ja) 2020-12-23

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JP2017555346A Active JP6804467B2 (ja) 2015-04-23 2016-04-25 組換え型ヌクレオシド特異的リボヌクレアーゼ及びその生成法と使用法
JP2020163486A Pending JP2021006049A (ja) 2015-04-23 2020-09-29 組換え型ヌクレオシド特異的リボヌクレアーゼ及びその生成法と使用法

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JP2020163486A Pending JP2021006049A (ja) 2015-04-23 2020-09-29 組換え型ヌクレオシド特異的リボヌクレアーゼ及びその生成法と使用法

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US (2) US10533163B2 (https=)
EP (1) EP3286305A4 (https=)
JP (2) JP6804467B2 (https=)
WO (1) WO2016172678A2 (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20260071268A1 (en) * 2024-09-09 2026-03-12 Waters Technologies Corporation Methods of inducing site-specific cleavage of rna polynucleotides with t2 family ribonucleases

Family Cites Families (2)

* Cited by examiner, † Cited by third party
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WO2008070179A2 (en) * 2006-12-06 2008-06-12 Monsanto Technology, Llc Genes and uses for plant improvement
WO2008103761A2 (en) * 2007-02-20 2008-08-28 Sequenom, Inc. Methods and compositions for cancer diagnosis and treatment based on nucleic acid methylation

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Publication number Publication date
JP2018512883A (ja) 2018-05-24
WO2016172678A2 (en) 2016-10-27
US10533163B2 (en) 2020-01-14
US20180320154A1 (en) 2018-11-08
US20200115689A1 (en) 2020-04-16
WO2016172678A3 (en) 2016-12-15
JP2021006049A (ja) 2021-01-21
US11535834B2 (en) 2022-12-27
EP3286305A2 (en) 2018-02-28
EP3286305A4 (en) 2018-09-12

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