JP6660181B2 - トリコデルマリーセイＱＭ６ａおよびその誘導体中の交配障害と関連する遺伝子／遺伝要素ならびにその同定方法 - Google Patents

トリコデルマリーセイＱＭ６ａおよびその誘導体中の交配障害と関連する遺伝子／遺伝要素ならびにその同定方法 Download PDF

Info

Publication number
JP6660181B2
JP6660181B2 JP2015550064A JP2015550064A JP6660181B2 JP 6660181 B2 JP6660181 B2 JP 6660181B2 JP 2015550064 A JP2015550064 A JP 2015550064A JP 2015550064 A JP2015550064 A JP 2015550064A JP 6660181 B2 JP6660181 B2 JP 6660181B2
Authority
JP
Japan
Prior art keywords
strain
qm6a
mating
gene
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2015550064A
Other languages
English (en)
Japanese (ja)
Other versions
JP2016501549A (ja
JP2016501549A5 (enExample
Inventor
クリスティアン・ピー・クビチェック
リタ・リンケ
ベルンハルド・セイボス
トマス・ハールマン
パトリック・ロレンツ
Original Assignee
アーベー・エンザイムス・ゲーエムベーハー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by アーベー・エンザイムス・ゲーエムベーハー filed Critical アーベー・エンザイムス・ゲーエムベーハー
Publication of JP2016501549A publication Critical patent/JP2016501549A/ja
Publication of JP2016501549A5 publication Critical patent/JP2016501549A5/ja
Application granted granted Critical
Publication of JP6660181B2 publication Critical patent/JP6660181B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungi isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/885—Trichoderma

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2015550064A 2012-12-28 2013-12-23 トリコデルマリーセイＱＭ６ａおよびその誘導体中の交配障害と関連する遺伝子／遺伝要素ならびにその同定方法 Expired - Fee Related JP6660181B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261746861P 2012-12-28 2012-12-28
EP12199606 2012-12-28
EP12199606.0 2012-12-28
US61/746,861 2012-12-28
PCT/EP2013/077910 WO2014102241A1 (en) 2012-12-28 2013-12-23 Genes/genetic elements associated with mating impairment in trichoderma reesei qm6a and its derivatives and process for their identification

Publications (3)

Publication Number Publication Date
JP2016501549A JP2016501549A (ja) 2016-01-21
JP2016501549A5 JP2016501549A5 (enExample) 2016-11-24
JP6660181B2 true JP6660181B2 (ja) 2020-03-11

Family

ID=47458776

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015550064A Expired - Fee Related JP6660181B2 (ja) 2012-12-28 2013-12-23 トリコデルマリーセイＱＭ６ａおよびその誘導体中の交配障害と関連する遺伝子／遺伝要素ならびにその同定方法

Country Status (7)

Country Link
US (1) US9920384B2 (enExample)
EP (1) EP2938727B1 (enExample)
JP (1) JP6660181B2 (enExample)
CN (1) CN105980565A (enExample)
CA (1) CA2891952A1 (enExample)
DK (1) DK2938727T3 (enExample)
WO (1) WO2014102241A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI570236B (zh) * 2013-10-02 2017-02-11 中央研究院 經有性雜交產生木黴菌節段性非整倍體（ｓａｎ）菌株之方法及所製得的ｓａｎ菌株
US20160304887A1 (en) * 2013-12-10 2016-10-20 Technische Universitat Wein Introducing or Inactivating Female Fertility in Filamentous Fungal Cells
DK3259364T3 (da) * 2015-02-20 2020-09-07 Roal Oy Fremgangsmåder til styring af proteasefremstilling
CN107365368B (zh) * 2016-05-11 2020-09-08 中国科学院微生物研究所 Asperphenamate合成相关蛋白及其编码基因与应用
FR3068710B1 (fr) * 2017-07-07 2025-08-15 Ifp Energies Now Procede de retablissement de la reproduction sexuee chez le champignon trichoderma reesei
CN114703202B (zh) * 2022-03-30 2023-05-26 四川省农业科学院植物保护研究所 敲除Pth11-rg1基因在提高淡紫紫孢菌对南方根结线虫致死效果的应用
WO2024118882A1 (en) * 2022-12-01 2024-06-06 Genencor International Bv Iterative multiplex genome engineering in microbial cells using a selection marker swapping system

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2531590A2 (en) 2010-02-04 2012-12-12 DSM IP Assets B.V. Process for preparing filamentous fungal strains having a sexual cycle and a process for preparing sexually crossed filamentous fungal strains
US20160304887A1 (en) 2013-12-10 2016-10-20 Technische Universitat Wein Introducing or Inactivating Female Fertility in Filamentous Fungal Cells

Also Published As

Publication number Publication date
CN105980565A (zh) 2016-09-28
CA2891952A1 (en) 2014-07-03
DK2938727T3 (en) 2019-01-14
WO2014102241A1 (en) 2014-07-03
JP2016501549A (ja) 2016-01-21
EP2938727A1 (en) 2015-11-04
US9920384B2 (en) 2018-03-20
EP2938727B1 (en) 2018-11-14
US20150361510A1 (en) 2015-12-17

Similar Documents

Publication Publication Date Title
JP6660181B2 (ja) トリコデルマリーセイＱＭ６ａおよびその誘導体中の交配障害と関連する遺伝子／遺伝要素ならびにその同定方法
US20240093241A1 (en) Crispr enabled multiplexed genome engineering
JP6937740B2 (ja) ゲノム編集システムおよび使用方法
JP6916188B2 (ja) プロトプラストからの全植物体の製造方法
JP7430189B2 (ja) 外来遺伝子を発現させるためのピキア・パストリス変異株
US8709766B2 (en) Use of endogenous promoters in genetic engineering of Nannochloropsis gaditana
Fu et al. PRM1 and KAR5 function in cell-cell fusion and karyogamy to drive distinct bisexual and unisexual cycles in the Cryptococcus pathogenic species complex
KR20170087521A (ko) 진균 게놈 변형 시스템 및 사용 방법
Linke et al. Restoration of female fertility in Trichoderma reesei QM6a provides the basis for inbreeding in this industrial cellulase producing fungus
KR20170121051A (ko) Ｒｇｅｎ ｒｎｐ를 이용한 미세조류의 교정 방법
US20220170041A1 (en) Method for cultivating plant resistant to gray leaf spot
JP2021521821A (ja) 粘性が低下した表現型を含む糸状菌株
WO1999027072A1 (en) Reagents and methods for diversification of dna
JP2018157814A (ja) シュードザイマ・アンタクティカの新規菌株
WO2012128260A1 (ja) シゾサッカロミセス属酵母の形質転換体、該形質転換体の製造方法、β－グルコシダーゼの製造方法、およびセルロースの分解方法
EP3412765B1 (en) Method for producing mutant filamentous fungi
JP2009027999A (ja) シス−アコニット酸デカルボキシラーゼをコードするｄｎａ、シス−アコニット酸デカルボキシラーゼの製造方法、及び、イタコン酸の製造方法
JP2018068292A (ja) 糸状菌の形質転換方法
CN103443268A (zh) 植物过氧化物酶在丝状真菌中的表达
Zhang et al. Identification and functional characterization of major gene pcmfs, controlling cap color formation in Pleurotus cornucopiae
Lou et al. Screening and functional verification of selectable marker genes for Cordyceps militaris
Evangelinos et al. Minos as a novel Tc1/mariner-type transposable element for functional genomic analysis in Aspergillus nidulans
JP2012223137A (ja) デンプン顆粒結合型デンプン合成酵素をコードする核酸、プライマー対、抑制剤およびモチ性ソバ属植物
JP7685012B2 (ja) エリスリトール資化能欠損変異トリコデルマ属菌、及びこれを用いた目的物質の製造方法
Class et al. Patent application title: GENES/GENETIC ELEMENTS ASSOCIATED WITH MATING IMPAIRMENT IN TRICHODERMA REESEI QM6A AND ITS DERIVATIVES AND PROCESS FOR THEIR IDENTIFICATION Inventors: Christian P. Kubicek (Wien, AT) Rita Linke (Wien, AT) Bernhard Seiboth (Wien, AT) Thomas Haarmann (Alsbach-Hahnlein, DE) Patrick Lorenz (Lorsch, DE)

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150821

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150930

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20161004

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20161004

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20170810

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170823

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20171121

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20180502

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180725

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20180727

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20180910

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20181116

RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7423

Effective date: 20190418

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20190418

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190905

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20200109

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20200207

R150 Certificate of patent or registration of utility model

Ref document number: 6660181

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

LAPS Cancellation because of no payment of annual fees