JP6562251B2 - 金属ナノ粒子組成物の製造方法 - Google Patents
金属ナノ粒子組成物の製造方法 Download PDFInfo
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Description
本発明に係る金属ナノ粒子組成物の製造方法は、
0℃のメタノールを抽出溶媒として、植物から植物抽出物を抽出する抽出ステップと、
前記植物抽出物と金属イオンとを混合し、撹拌して混合溶液を調製する調製ステップと、
を含む。
前記調製ステップでは、
前記混合溶液のpHを中性に近づけるpH調整剤を混合する、
こととしてもよい。
炭酸水素ナトリウム水溶液である、
こととしてもよい。
茶葉であって、
前記植物抽出物は、
茶抽出物である、
こととしてもよい。
実施の形態1について詳細に説明する。本実施の形態に係る金属ナノ粒子組成物の製造方法は、抽出ステップと、調製ステップと、を含む。以下、各ステップについて説明する。
次に、実施の形態2について説明する。本実施の形態は、上記実施の形態1と抽出ステップのみが異なる。以下では、上記実施の形態1と異なる点について主に説明する。
さらに、実施の形態3について説明する。本実施の形態に係る金属ナノ粒子組成物の製造方法は、抽出ステップと調製ステップとを含む。以下では、上記実施の形態1と異なる点について主に説明する。
実施の形態4について説明する。本実施の形態に係る抗菌剤は、上記各実施の形態で説明した金属ナノ粒子組成物を含有する。該金属ナノ粒子組成物は、金属ナノ粒子の表面に植物抽出物が集積されているので、植物抽出物の機能を有する。植物抽出物であるカテキン、カフェイン、サポニン、テアニンおよびビタミンCなどは、細菌などの増殖を抑制する、もしくは、抗菌作用または殺菌作用を有する。また、当該植物抽出物は、抗ウイルス作用を有する。
実施の形態5について説明する。本実施の形態に係る染料は、上記各実施の形態で説明した金属ナノ粒子組成物を含有する。該金属ナノ粒子組成物は、金属ナノ粒子の光学的な特性を有するため、特徴的な色を呈する。
10gの煎茶用茶葉(鹿児島産、お茶の沢田園社製)に65℃のイオン交換水を100mL加え、5分後に濾過した。濾液を煎茶−水抽出液とした。
煎茶−水抽出液100mLに、10−2Mの炭酸水素ナトリウム水溶液80mLを加え、さらに、10−2Mの硝酸銀水溶液20mLを加え、激しく攪拌した。これにより、銀ナノ粒子組成物(実施例1)溶液を得た。
上記非特許文献1と同様に、煎茶用茶葉を用いた金ナノ粒子組成物(比較例1)溶液を次のように調製した。1.5gの煎茶用茶葉をイオン交換水100mLに入れ沸騰させた。得られた抽出液4mLに、10−3Mの塩化金(III)酸水溶液を5mL加え、さらにイオン交換水を1mL加え、10mLとした。
あらかじめ煮沸殺菌し乾燥させた木綿繊維を、実施例1〜6の溶液に浸し、24時間静置した。その後、木綿繊維を取り出し風乾し、イオン交換水で洗浄した。この結果、実施例1〜6は木綿繊維上に担持され、木綿繊維に着色した。
実施例1〜6を担持させた木綿繊維の殺菌活性を評価した。また、木綿繊維と同様に羊毛に実施例1〜4を担持させ、当該羊毛の殺菌活性を評価した。殺菌活性は、JIS L 1902:2008「繊維製品の抗菌性試験および抗菌効果」10 定量試験 10.1 菌液吸収法で評価した。生菌数は、混釈平板培養法で測定した。使用した菌は、グラム陽性菌である黄色ブドウ球菌およびグラム陰性菌である肺炎かん菌である。
表2は、黄色ブドウ球菌に対する実施例1〜6の殺菌活性を示す。生菌数の「<20」は、生菌を検出しなかったことを示す。また、本試験では、殺菌活性値が0以上の場合、殺菌活性を認めるといえる。木綿繊維に担持させた場合、実施例1〜6は、いずれも非常に高い殺菌活性を示した。一方、羊毛に担持させた場合、実施例1〜4は、殺菌活性を示さなかった。
煎茶用茶葉10gに100℃のイオン交換水を100mL加え、5分後に濾過した。この操作をさらに2回繰り返した。3回目の操作で得られた煎茶−水抽出液を用いて、実施例1の調製と同様に、煎茶−金ナノ粒子組成物(実施例7)溶液を得た。
図7は、調製後24時間経過した実施例2、実施例6、比較例1および比較例2の溶液を示す。比較例1では、塩化金(III)酸水溶液を添加した直後に溶液の色の変化が見られたが、24時間後は沈澱が生じた。比較例2でも同様に沈澱が生じた。比較例1および比較例2の調製方法では、金ナノ粒子が凝集し、金ナノ粒子よりも大きな不溶性の金属の塊となったため、沈殿が生じたと考えられる。
Claims (4)
- 0℃のメタノールを抽出溶媒として、植物から植物抽出物を抽出する抽出ステップと、
前記植物抽出物と金属イオンとを混合し、撹拌して混合溶液を調製する調製ステップと、
を含む、金属ナノ粒子組成物の製造方法。 - 前記調製ステップでは、
前記混合溶液のpHを中性に近づけるpH調整剤を混合する、
請求項1に記載の金属ナノ粒子組成物の製造方法。 - 前記pH調整剤は、
炭酸水素ナトリウム水溶液である、
請求項2に記載の金属ナノ粒子組成物の製造方法。 - 前記植物は、
茶葉であって、
前記植物抽出物は、
茶抽出物である、
請求項1から3のいずれか一項に記載の金属ナノ粒子組成物の製造方法。
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