JP6542676B2 - ナノフルイディクスにおける分子の特性解析 - Google Patents

ナノフルイディクスにおける分子の特性解析 Download PDF

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JP6542676B2
JP6542676B2 JP2015558929A JP2015558929A JP6542676B2 JP 6542676 B2 JP6542676 B2 JP 6542676B2 JP 2015558929 A JP2015558929 A JP 2015558929A JP 2015558929 A JP2015558929 A JP 2015558929A JP 6542676 B2 JP6542676 B2 JP 6542676B2
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JP2016510590A5 (enExample
JP2016510590A (ja
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ハン カオ、
ハン カオ、
アレックス、 アール． ハスティー、
アレックス、 アール． ハスティー、
アーネスト、 チ‐ツン ラム、
アーネスト、 チ‐ツン ラム、
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バイオナノ ジェノミクス、 インコーポレイテッド
バイオナノ ジェノミクス、 インコーポレイテッド
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    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Physiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2015558929A 2013-02-20 2014-02-19 ナノフルイディクスにおける分子の特性解析 Active JP6542676B2 (ja)

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US201361767219P 2013-02-20 2013-02-20
US61/767,219 2013-02-20
PCT/US2014/017226 WO2014130589A1 (en) 2013-02-20 2014-02-19 Characterization of molecules in nanofluidics

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US (4) US9809855B2 (enExample)
EP (1) EP2959015B1 (enExample)
JP (2) JP6542676B2 (enExample)
CN (1) CN105229168B (enExample)
AU (1) AU2014219001A1 (enExample)
CA (1) CA2901460A1 (enExample)
WO (1) WO2014130589A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10844424B2 (en) 2013-02-20 2020-11-24 Bionano Genomics, Inc. Reduction of bias in genomic coverage measurements
CN105229168B (zh) 2013-02-20 2020-07-17 生物纳米基因有限公司 纳米流体中分子的表征
CN106164295B (zh) 2014-02-25 2020-08-11 生物纳米基因公司 减小基因组覆盖测量中的偏差
WO2018148903A1 (zh) * 2017-02-16 2018-08-23 上海亿康医学检验所有限公司 泌尿系统肿瘤的辅助诊断方法
DE102019200929A1 (de) * 2019-01-25 2020-07-30 Robert Bosch Gmbh Verfahren und Vorrichtung zum optischen Erfassen einer Länge eines Makromoleküls
JP2023554621A (ja) * 2020-12-15 2023-12-28 クアンタム－エスアイ インコーポレイテッド 超高感度バイオセンサ法
WO2024145268A2 (en) 2022-12-25 2024-07-04 Bionano Genomics, Inc. Optical genome mapping cartridges
EP4639178A1 (en) 2022-12-25 2025-10-29 Bionano Genomics, Inc. Optical genome mapping system
EP4639177A1 (en) 2022-12-25 2025-10-29 Bionano Genomics, Inc. Instrumentation of optical genome mapping systems

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030104428A1 (en) 2001-06-21 2003-06-05 President And Fellows Of Harvard College Method for characterization of nucleic acid molecules
SG169225A1 (en) * 2001-07-25 2011-03-30 Univ Princeton Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
AU2003243691A1 (en) 2002-06-20 2004-01-06 Primera Biosystems, Inc. Apparatus for polynucleotide detection and quantitation
US8346482B2 (en) * 2003-08-22 2013-01-01 Fernandez Dennis S Integrated biosensor and simulation system for diagnosis and therapy
US20100216153A1 (en) * 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
US20060063184A1 (en) 2004-09-09 2006-03-23 Felix Carolyn A Compositions and methods for the detection of DNA topoisomerase II complexes with DNA
JP2009529887A (ja) * 2006-03-13 2009-08-27 イコニシス インコーポレーテッド 胎児細胞およびガン細胞を診断する非侵襲的方法
EP3978121A1 (en) 2006-07-19 2022-04-06 BioNano Genomics, Inc. Nanonozzle device arrays: their preparation and use for macromolecular analysis
US8722327B2 (en) * 2007-03-28 2014-05-13 Bionano Genomics, Inc. Methods of macromolecular analysis using nanochannel arrays
EP3892736A1 (en) * 2007-07-23 2021-10-13 The Chinese University of Hong Kong Determining a nucleic acid sequence imbalance
US20090076345A1 (en) 2007-09-14 2009-03-19 Corventis, Inc. Adherent Device with Multiple Physiological Sensors
JP4831236B2 (ja) 2007-11-12 2011-12-07 株式会社村田製作所 操作位置検出器およびその応用装置
KR20110016479A (ko) 2008-06-06 2011-02-17 바이오나노매트릭스, 인크. 통합 나노유체 분석 장치, 제작 방법 및 분석 기술
US8628919B2 (en) * 2008-06-30 2014-01-14 Bionano Genomics, Inc. Methods and devices for single-molecule whole genome analysis
WO2011050147A1 (en) 2009-10-21 2011-04-28 Bionanomatrix, Inc . Methods and related devices for single molecule whole genome analysis
JP5846703B2 (ja) 2008-11-18 2016-01-20 バイオナノ ジェノミックス、インク． ポリヌクレオチドのマッピング及び配列決定
US20100137154A1 (en) * 2008-12-01 2010-06-03 Ach Robert A Genome analysis using a methyltransferase
US20120097835A1 (en) 2009-05-19 2012-04-26 BioNano Geneomics, Inc. Devices and methods for dynamic determination of sample position and orientation and dynamic repositioning
US9725315B2 (en) 2009-09-28 2017-08-08 Bionano Genomics, Inc. Nanochannel arrays and near-field illumination devices for polymer analysis and related methods
SG189778A1 (en) * 2009-10-20 2013-05-31 Agency Science Tech & Res Microfluidic system for trapping and detection of a biological entity in a sample
US10388403B2 (en) 2010-01-19 2019-08-20 Verinata Health, Inc. Analyzing copy number variation in the detection of cancer
KR20130000396A (ko) * 2010-03-04 2013-01-02 메사추세츠 인스티튜트 오브 테크놀로지 세포 검출 및 단리를 위한 마이크로유체 선별기
EP2547795B1 (en) 2010-03-16 2015-01-14 Nanostring Technologies, Inc Compositions and methods for the detection of genomic features
IT1399366B1 (it) 2010-04-08 2013-04-16 Weightpack S P A Camma variabile, particolarmente per macchine per l'applicazione di tappi.
US8725422B2 (en) 2010-10-13 2014-05-13 Complete Genomics, Inc. Methods for estimating genome-wide copy number variations
SG10201508373UA (en) 2010-10-20 2015-11-27 Bionano Genomics Inc Systems and methods for assessing biomolecule characteristics
BR112013020220B1 (pt) 2011-02-09 2020-03-17 Natera, Inc. Método para determinar o estado de ploidia de um cromossomo em um feto em gestação
WO2014014498A1 (en) 2012-07-20 2014-01-23 Verinata Health, Inc. Detecting and classifying copy number variation in a fetal genome
KR101489568B1 (ko) * 2011-06-29 2015-02-03 비지아이 헬스 서비스 코포레이션 리미티드 태아 유전학적 이상의 비침습성 검출
US20130072386A1 (en) 2011-09-08 2013-03-21 Bionano Genomics, Inc. Physical map construction of whole genome and pooled clone mapping in nanochannel array
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9367663B2 (en) 2011-10-06 2016-06-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
JP6073902B2 (ja) 2011-10-06 2017-02-01 セクエノム， インコーポレイテッド 遺伝的変異の非侵襲的評価のための方法およびプロセス
CA2850785C (en) 2011-10-06 2022-12-13 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
US8688388B2 (en) 2011-10-11 2014-04-01 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
DK2766496T3 (en) 2011-10-11 2017-05-15 Sequenom Inc METHODS AND PROCESSES FOR NON-INVASIVE ASSESSMENT OF GENETIC VARIATIONS
CA2861856C (en) 2012-01-20 2020-06-02 Sequenom, Inc. Diagnostic processes that factor experimental conditions
EP2844771A4 (en) 2012-05-04 2015-12-02 Complete Genomics Inc METHOD FOR DETERMINING THE ABSOLUTE GENOME-WIDE COPY COUNTER CHANGES OF COMPLEX TUMORS
ES2902401T3 (es) 2012-05-21 2022-03-28 Sequenom Inc Métodos y procesos para la evaluación no invasiva de variaciones genéticas
US10497461B2 (en) 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9347127B2 (en) 2012-07-16 2016-05-24 Veeco Instruments, Inc. Film deposition assisted by angular selective etch on a surface
US20130309666A1 (en) 2013-01-25 2013-11-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CN105143462A (zh) 2013-02-05 2015-12-09 生物纳米基因有限公司 用于单分子分析的方法
CN105229168B (zh) 2013-02-20 2020-07-17 生物纳米基因有限公司 纳米流体中分子的表征

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EP2959015A1 (en) 2015-12-30
US20200362413A1 (en) 2020-11-19
US10669586B2 (en) 2020-06-02
JP6928032B2 (ja) 2021-09-01
US20220340973A1 (en) 2022-10-27
US20180223361A1 (en) 2018-08-09
JP2019205434A (ja) 2019-12-05
EP2959015B1 (en) 2020-11-04
CN105229168B (zh) 2020-07-17
US20160046992A1 (en) 2016-02-18
US9809855B2 (en) 2017-11-07
EP2959015A4 (en) 2016-08-17
AU2014219001A1 (en) 2015-10-08
HK1219511A1 (zh) 2017-04-07
US12275992B2 (en) 2025-04-15
JP2016510590A (ja) 2016-04-11
WO2014130589A1 (en) 2014-08-28
CA2901460A1 (en) 2014-08-28
CN105229168A (zh) 2016-01-06
US11359244B2 (en) 2022-06-14

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