JP6511720B2 - コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法 - Google Patents

コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法 Download PDF

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JP6511720B2
JP6511720B2 JP2014031571A JP2014031571A JP6511720B2 JP 6511720 B2 JP6511720 B2 JP 6511720B2 JP 2014031571 A JP2014031571 A JP 2014031571A JP 2014031571 A JP2014031571 A JP 2014031571A JP 6511720 B2 JP6511720 B2 JP 6511720B2
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polynucleotide
protein
seq
sequence
nucleotide sequence
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JP2014193147A5 (https=
JP2014193147A (ja
Inventor
井上　敏
井上　　敏
佐藤　淳一
淳一 佐藤
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JNC Corp
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JNC Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12013—Oplophorus-luciferin 2-monooxygenase (1.13.12.13)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2014031571A 2013-02-28 2014-02-21 コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法 Active JP6511720B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2014031571A JP6511720B2 (ja) 2013-02-28 2014-02-21 コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2013038350 2013-02-28
JP2013038350 2013-02-28
JP2014031571A JP6511720B2 (ja) 2013-02-28 2014-02-21 コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法

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JP2014193147A JP2014193147A (ja) 2014-10-09
JP2014193147A5 JP2014193147A5 (https=) 2017-01-12
JP6511720B2 true JP6511720B2 (ja) 2019-05-15

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JP2018207131A Expired - Fee Related JP6777133B2 (ja) 2013-02-28 2018-11-02 コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法

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US (4) US9315783B2 (https=)
JP (2) JP6511720B2 (https=)
GB (2) GB2516326B (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6040846B2 (ja) * 2013-04-08 2016-12-07 Jnc株式会社 セレンテラジン類縁体
JP6442825B2 (ja) * 2013-12-26 2018-12-26 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
JP6824594B2 (ja) * 2014-09-11 2021-02-03 Jnc株式会社 合成遺伝子の設計方法
GB201702500D0 (en) * 2017-02-16 2017-04-05 Univ Sheffield Stable vamp reporter assay
EP3395803A1 (en) * 2017-04-28 2018-10-31 Institut Pasteur Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins
US20230366023A1 (en) 2020-10-09 2023-11-16 Bgi Shenzhen Co., Ltd. Methods and compositions for bioluminescence-based sequencing
US11827908B2 (en) 2021-02-12 2023-11-28 Shimadzu Corporation Polypeptide having luciferase activity
JP7725866B2 (ja) 2021-05-19 2025-08-20 株式会社島津製作所 分子間相互作用検出に用いられるポリペプチドを含む試薬キット

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1156103B1 (en) * 2000-04-26 2010-08-11 Chisso Corporation Oplophorus luciferase
JP4613441B2 (ja) 2000-04-26 2011-01-19 チッソ株式会社 新規ルシフェラーゼおよび発光蛋白質
US7268229B2 (en) 2001-11-02 2007-09-11 Promega Corporation Compounds to co-localize luminophores with luminescent proteins
AU2003301883A1 (en) 2002-10-30 2004-06-07 University Of Tennessee Research Foundation Modified luciferase nucleic acids and methods of use
JP4609177B2 (ja) 2005-04-28 2011-01-12 チッソ株式会社 カルシウム結合型発光蛋白質溶液の発光時間延長方法
JP5176292B2 (ja) 2006-06-22 2013-04-03 Ｊｎｃ株式会社 発光活性測定方法
US7723502B2 (en) 2008-10-10 2010-05-25 Marker Gene Technologies, Inc Modified Luciola cruciata luciferase gene and protein
GB2479847B (en) 2009-02-09 2014-02-19 Jnc Corp Coelenterazine analogs and manufacturing method thereof
LT3409764T (lt) 2009-05-01 2020-06-10 Promega Corporation Sintetinės oplophorus liuciferazės su sustiprinta šviesos išeiga
EP2454261B1 (en) 2009-07-13 2014-11-19 Universita Degli Studi di Siena Imidazo[1,2-alpha]pyrazin-3(7h)-one derivatives bearing a new electron-rich structure
US9353401B2 (en) 2009-08-29 2016-05-31 Targeting Systems Multiplex assays with multiple luciferases reporters and uses thereof
BR112013010487B1 (pt) * 2010-11-02 2021-02-02 Promega Corporation compostos de coelenterazina, kit compreendendo ditos compostos e método para detectar luminescência em uma amostra in vitro
JP5896624B2 (ja) * 2011-05-13 2016-03-30 オリンパス株式会社 ホタル由来ルシフェラーゼ

Also Published As

Publication number Publication date
JP6777133B2 (ja) 2020-10-28
US20140242574A1 (en) 2014-08-28
JP2019037246A (ja) 2019-03-14
GB201913094D0 (en) 2019-10-23
GB2574745A (en) 2019-12-18
US9506104B2 (en) 2016-11-29
GB2516326A (en) 2015-01-21
US9315783B2 (en) 2016-04-19
GB2574745B (en) 2020-09-16
US20170029789A1 (en) 2017-02-02
US20160186234A1 (en) 2016-06-30
US10006009B2 (en) 2018-06-26
GB201403374D0 (en) 2014-04-09
GB2516326B (en) 2020-06-03
US9404145B2 (en) 2016-08-02
US20160298171A1 (en) 2016-10-13
JP2014193147A (ja) 2014-10-09

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