JP6480926B2 - タンパク質におけるフコシル化レベルを制御するための方法 - Google Patents
タンパク質におけるフコシル化レベルを制御するための方法 Download PDFInfo
- Publication number
- JP6480926B2 JP6480926B2 JP2016528640A JP2016528640A JP6480926B2 JP 6480926 B2 JP6480926 B2 JP 6480926B2 JP 2016528640 A JP2016528640 A JP 2016528640A JP 2016528640 A JP2016528640 A JP 2016528640A JP 6480926 B2 JP6480926 B2 JP 6480926B2
- Authority
- JP
- Japan
- Prior art keywords
- manganese
- antibody
- cells
- culture medium
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/18—Antivirals for RNA viruses for HIV
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2896—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
- C07K2317/41—Glycosylation, sialylation, or fucosylation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/54—F(ab')2
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/72—Increased effector function due to an Fc-modification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
- C07K2317/732—Antibody-dependent cellular cytotoxicity [ADCC]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
- C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- AIDS & HIV (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Saccharide Compounds (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN3262/CHE/2013 | 2013-07-23 | ||
| IN3262CH2013 | 2013-07-23 | ||
| IN3265/CHE/2013 | 2013-07-23 | ||
| IN3265CH2013 | 2013-07-23 | ||
| PCT/IB2014/063348 WO2015011660A1 (en) | 2013-07-23 | 2014-07-23 | Methods for controlling fucosylation levels in proteins |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016525352A JP2016525352A (ja) | 2016-08-25 |
| JP2016525352A5 JP2016525352A5 (enExample) | 2017-06-22 |
| JP6480926B2 true JP6480926B2 (ja) | 2019-03-13 |
Family
ID=52392808
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016528640A Active JP6480926B2 (ja) | 2013-07-23 | 2014-07-23 | タンパク質におけるフコシル化レベルを制御するための方法 |
Country Status (19)
| Country | Link |
|---|---|
| US (2) | US9856502B2 (enExample) |
| EP (2) | EP4282975A3 (enExample) |
| JP (1) | JP6480926B2 (enExample) |
| KR (1) | KR102062784B1 (enExample) |
| CN (1) | CN105392878A (enExample) |
| AU (1) | AU2014294618B2 (enExample) |
| BR (1) | BR112016001461A2 (enExample) |
| CA (1) | CA2926967C (enExample) |
| CU (1) | CU24312B1 (enExample) |
| EA (1) | EA201690269A1 (enExample) |
| HK (1) | HK1220228A1 (enExample) |
| MX (1) | MX2016001042A (enExample) |
| NZ (1) | NZ716182A (enExample) |
| PE (1) | PE20160218A1 (enExample) |
| PH (1) | PH12016500158A1 (enExample) |
| SG (1) | SG11201600067YA (enExample) |
| TW (1) | TWI621711B (enExample) |
| WO (1) | WO2015011660A1 (enExample) |
| ZA (1) | ZA201601113B (enExample) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT2993186T (pt) | 2008-03-14 | 2019-11-29 | Biocon Ltd | Anticorpo monoclonal e um método do mesmo |
| CA2969225C (en) | 2014-12-01 | 2023-08-22 | Amgen Inc. | Process for manipulating the level of glycan content of a glycoprotein |
| KR102514528B1 (ko) * | 2016-10-21 | 2023-03-27 | 바이오콘 리미티드 | 루푸스 치료를 위한 단일클론항체 및 이의 치료방법 |
| EP3541835B1 (en) * | 2016-11-18 | 2026-05-13 | Biocon Limited | Rapid and efficient de-glycosylation of glycoproteins |
| EP3824906A1 (en) | 2016-12-21 | 2021-05-26 | Amgen Inc. | Anti-tnf alpha antibody formulations |
| AU2018235928B2 (en) | 2017-03-14 | 2023-09-21 | Amgen Inc. | Control of total afucosylated glycoforms of antibodies produced in cell culture |
| US12325737B2 (en) * | 2018-03-26 | 2025-06-10 | Amgen Inc. | Total afucosylated glycoforms of antibodies produced in cell culture |
| KR102742590B1 (ko) * | 2018-04-20 | 2024-12-16 | (주)셀트리온 | 당단백질의 푸코실레이션을 조절하는 방법 |
| CA3107038A1 (en) * | 2018-08-10 | 2020-02-13 | Genentech, Inc. | Cell culture strategies for modulating protein glycosylation |
| HU231514B1 (hu) | 2018-11-07 | 2024-07-28 | Richter Gedeon Nyrt. | Sejttenyészetben előállított rekombináns glikoprotein glikozilációs mintázatának megváltoztatására szolgáló módszer |
| CN113015805B (zh) | 2018-11-13 | 2024-12-03 | 詹森生物科技公司 | 抗cd38抗体产生期间的痕量金属的控制 |
| JP7469593B2 (ja) * | 2018-12-27 | 2024-04-17 | 東ソー株式会社 | 抗体依存性細胞傷害活性が向上した抗体を製造する方法 |
| CN112779307B (zh) * | 2021-01-11 | 2022-04-19 | 苏州药明生物技术有限公司 | 一种两阶段调节cho表达外源蛋白糖型的方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| US6548640B1 (en) | 1986-03-27 | 2003-04-15 | Btg International Limited | Altered antibodies |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| FI903489A7 (fi) | 1988-11-11 | 1990-07-10 | Medical Res Council | Yhden osan sisältävät ligandit, näitä ligandeja sisältävät reseptorit, menetelmiä niiden valmistamiseksi sekä ligandien ja reseptorien käytt ö |
| US5859205A (en) | 1989-12-21 | 1999-01-12 | Celltech Limited | Humanised antibodies |
| EP0859841B1 (en) | 1995-08-18 | 2002-06-19 | MorphoSys AG | Protein/(poly)peptide libraries |
| AU747898B2 (en) * | 1997-03-03 | 2002-05-30 | Bristol-Myers Squibb Company | Monoclonal antibodies to human CD6 |
| ATE306810T1 (de) | 2002-03-05 | 2005-11-15 | Artemis Pharmaceuticals Gmbh | Durch inzucht erzeugte von embryonalen stammzellen abgeleitete mäuse |
| KR20080032065A (ko) | 2005-06-03 | 2008-04-14 | 제넨테크, 인크. | 푸코실화 수준이 조절된 항체의 생성 방법 |
| EP3026109B1 (en) | 2005-12-08 | 2022-03-09 | Amgen Inc. | Improved production of glycoproteins using manganese |
| US7846724B2 (en) * | 2006-04-11 | 2010-12-07 | Hoffmann-La Roche Inc. | Method for selecting CHO cell for production of glycosylated antibodies |
| PT2993186T (pt) * | 2008-03-14 | 2019-11-29 | Biocon Ltd | Anticorpo monoclonal e um método do mesmo |
| WO2010141855A1 (en) * | 2009-06-05 | 2010-12-09 | Momenta Pharmaceuticals, Inc. | Methods of modulating fucosylation of glycoproteins |
| AU2011237442A1 (en) * | 2010-04-07 | 2012-10-18 | Momenta Pharmaceuticals, Inc. | High mannose glycans |
| EP2809773B1 (en) * | 2012-01-30 | 2020-09-02 | Dr. Reddy's Laboratories Limited | Process of modulating man5 and/or afucosylation content of glycoprotein composition |
-
2014
- 2014-07-23 JP JP2016528640A patent/JP6480926B2/ja active Active
- 2014-07-23 CA CA2926967A patent/CA2926967C/en active Active
- 2014-07-23 SG SG11201600067YA patent/SG11201600067YA/en unknown
- 2014-07-23 AU AU2014294618A patent/AU2014294618B2/en active Active
- 2014-07-23 MX MX2016001042A patent/MX2016001042A/es unknown
- 2014-07-23 EA EA201690269A patent/EA201690269A1/ru unknown
- 2014-07-23 BR BR112016001461A patent/BR112016001461A2/pt not_active IP Right Cessation
- 2014-07-23 EP EP23196563.3A patent/EP4282975A3/en active Pending
- 2014-07-23 KR KR1020167004763A patent/KR102062784B1/ko active Active
- 2014-07-23 NZ NZ716182A patent/NZ716182A/en unknown
- 2014-07-23 US US14/905,350 patent/US9856502B2/en not_active Expired - Fee Related
- 2014-07-23 PE PE2016000087A patent/PE20160218A1/es not_active Application Discontinuation
- 2014-07-23 HK HK16108338.6A patent/HK1220228A1/zh unknown
- 2014-07-23 CU CUP2016000008A patent/CU24312B1/xx unknown
- 2014-07-23 EP EP14830053.6A patent/EP3024922A4/en not_active Ceased
- 2014-07-23 CN CN201480041292.1A patent/CN105392878A/zh active Pending
- 2014-07-23 WO PCT/IB2014/063348 patent/WO2015011660A1/en not_active Ceased
- 2014-07-24 TW TW103125274A patent/TWI621711B/zh not_active IP Right Cessation
-
2016
- 2016-01-22 PH PH12016500158A patent/PH12016500158A1/en unknown
- 2016-02-18 ZA ZA2016/01113A patent/ZA201601113B/en unknown
-
2017
- 2017-11-21 US US15/819,351 patent/US10308970B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EA201690269A1 (ru) | 2016-05-31 |
| WO2015011660A1 (en) | 2015-01-29 |
| KR20160036589A (ko) | 2016-04-04 |
| EP4282975A2 (en) | 2023-11-29 |
| HK1220228A1 (zh) | 2017-04-28 |
| CA2926967A1 (en) | 2015-01-29 |
| CA2926967C (en) | 2020-12-08 |
| AU2014294618B2 (en) | 2019-06-27 |
| EP4282975A3 (en) | 2024-02-28 |
| BR112016001461A2 (pt) | 2017-08-29 |
| JP2016525352A (ja) | 2016-08-25 |
| TWI621711B (zh) | 2018-04-21 |
| CN105392878A (zh) | 2016-03-09 |
| SG11201600067YA (en) | 2016-02-26 |
| US20180087080A1 (en) | 2018-03-29 |
| US20160138065A1 (en) | 2016-05-19 |
| EP3024922A4 (en) | 2017-03-29 |
| NZ716182A (en) | 2018-07-27 |
| TW201512396A (zh) | 2015-04-01 |
| PH12016500158A1 (en) | 2016-04-25 |
| MX2016001042A (es) | 2017-01-05 |
| EP3024922A1 (en) | 2016-06-01 |
| US9856502B2 (en) | 2018-01-02 |
| PE20160218A1 (es) | 2016-05-09 |
| AU2014294618A1 (en) | 2016-02-11 |
| ZA201601113B (en) | 2017-08-30 |
| CU20160008A7 (es) | 2016-11-29 |
| CU24312B1 (es) | 2018-02-08 |
| KR102062784B1 (ko) | 2020-01-07 |
| US10308970B2 (en) | 2019-06-04 |
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