JP6449266B2 - 流体ピックアップを備えるマイクロ流体システム - Google Patents

流体ピックアップを備えるマイクロ流体システム Download PDF

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JP6449266B2
JP6449266B2 JP2016521890A JP2016521890A JP6449266B2 JP 6449266 B2 JP6449266 B2 JP 6449266B2 JP 2016521890 A JP2016521890 A JP 2016521890A JP 2016521890 A JP2016521890 A JP 2016521890A JP 6449266 B2 JP6449266 B2 JP 6449266B2
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JP2016528027A5 (cg-RX-API-DMAC7.html
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アンソニー・ジェイ・マカレヴィツ・ジュニア
リュック・ブッス
ステファノ・シアフィーノ
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • BPERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • BPERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
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    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1075Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02Adapting objects or devices to another
    • B01L2200/026Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/04Closures and closing means
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/04Closures and closing means
    • B01L2300/041Connecting closures to device or container
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/04Closures and closing means
    • B01L2300/041Connecting closures to device or container
    • B01L2300/044Connecting closures to device or container pierceable, e.g. films, membranes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/04Closures and closing means
    • B01L2300/046Function or devices integrated in the closure
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0672Integrated piercing tool
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0829Multi-well plates; Microtitration plates
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • BPERFORMING OPERATIONS; TRANSPORTING
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    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0457Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2400/0633Valves, specific forms thereof with moving parts
    • B01L2400/0655Valves, specific forms thereof with moving parts pinch valves
    • BPERFORMING OPERATIONS; TRANSPORTING
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    • B01L2400/06Valves, specific forms thereof
    • B01L2400/0677Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B01L2400/084Passive control of flow resistance
    • BPERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/25Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575Volumetric liquid transfer

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  • Chemical & Material Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Dispersion Chemistry (AREA)
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  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Accessories For Mixers (AREA)
  • Colloid Chemistry (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Micromachines (AREA)
JP2016521890A 2013-06-21 2014-06-23 流体ピックアップを備えるマイクロ流体システム Active JP6449266B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361838063P 2013-06-21 2013-06-21
US61/838,063 2013-06-21
PCT/US2014/043715 WO2014205447A2 (en) 2013-06-21 2014-06-23 Microfluidic system with fluid pickups

Related Child Applications (1)

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JP2018228253A Division JP6790044B2 (ja) 2013-06-21 2018-12-05 流体ピックアップを備えるマイクロ流体システム

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JP2016528027A JP2016528027A (ja) 2016-09-15
JP2016528027A5 JP2016528027A5 (cg-RX-API-DMAC7.html) 2017-07-27
JP6449266B2 true JP6449266B2 (ja) 2019-01-09

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JP2016521890A Active JP6449266B2 (ja) 2013-06-21 2014-06-23 流体ピックアップを備えるマイクロ流体システム
JP2018228253A Active JP6790044B2 (ja) 2013-06-21 2018-12-05 流体ピックアップを備えるマイクロ流体システム
JP2020184645A Active JP7110300B2 (ja) 2013-06-21 2020-11-04 流体ピックアップを備えるマイクロ流体システム

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JP2020184645A Active JP7110300B2 (ja) 2013-06-21 2020-11-04 流体ピックアップを備えるマイクロ流体システム

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US (3) US9409174B2 (cg-RX-API-DMAC7.html)
EP (1) EP3011302B1 (cg-RX-API-DMAC7.html)
JP (3) JP6449266B2 (cg-RX-API-DMAC7.html)
CN (2) CN109738224B (cg-RX-API-DMAC7.html)
AU (1) AU2014284148B2 (cg-RX-API-DMAC7.html)
SG (1) SG11201510439PA (cg-RX-API-DMAC7.html)
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Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003000840A2 (en) 2001-06-21 2003-01-03 Diversa Corporation Nitrilases
US20040002147A1 (en) 1999-12-29 2004-01-01 Desantis Grace Nitrilases
CA2833423C (en) 2001-02-21 2019-03-19 Verenium Corporation Enzymes having alpha amylase activity and methods of use thereof
WO2003089620A2 (en) 2002-04-19 2003-10-30 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
CN101967490B (zh) 2002-06-14 2014-07-16 先正达公司 木聚糖酶、编码木聚糖酶的核酸以及制备和应用它们的方法
PL3023498T3 (pl) 2003-03-06 2019-05-31 Basf Enzymes Llc Amylazy, kodujące je kwasy nukleinowe i sposoby ich wytwarzania i zastosowania
PL2341136T3 (pl) 2003-04-04 2016-12-30 Liazy pektynianowe, kodujące je kwasy nukleinowe oraz sposoby ich wytwarzania i zastosowania
EP2404929B1 (en) 2003-07-02 2014-06-18 BP Corporation North America Inc. Glucanases, nucleic acids encoding them and methods for making and using them
CA2891101A1 (en) 2003-08-11 2005-03-10 Basf Enzymes Llc Laccases, nucleic acids encoding them and methods for making and using them
PL2468853T3 (pl) 2004-06-16 2015-08-31 Dsm Ip Assets Bv Sposób enzymatycznego odbarwiania feofityny
CA2614769A1 (en) 2005-03-10 2006-09-21 Verenium Corporation Lyase enzymes, nucleic acids encoding them and methods for making and using them
AU2006227965B2 (en) 2005-03-15 2013-01-31 Bp Corporation North America Inc. Cellulases, nucleic acids encoding them and methods for making and using them
NZ595502A (en) 2006-02-10 2013-11-29 Verenium Corp Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
ES2682284T3 (es) 2006-02-14 2018-09-19 Bp Corporation North America Inc. Xilanasas, ácidos nucleicos que las codifican y métodos para elaborarlas y usarlas
WO2007103389A2 (en) 2006-03-07 2007-09-13 Cargill, Incorporated Aldolases, nucleic acids encoding them and methods for making and using them
EA019971B1 (ru) 2006-03-07 2014-07-30 Верениум Корпорейшн Альдолазы, кодирующие их нуклеиновые кислоты и способы их получения и применения
AU2007356171B8 (en) 2006-08-04 2014-01-16 Bp Corporation North America Inc. Glucanases, nucleic acids encoding them, and methods for making and using them
KR20090068266A (ko) 2006-09-21 2009-06-25 베레늄 코포레이션 포스포리파제, 그를 코딩하는 핵산 그리고 그를 제조 및 이용하는 방법
ES2575912T3 (es) 2006-12-21 2016-07-04 Basf Enzymes Llc Amilasas y glucoamilasas, ácidos nucleicos que las codifican y métodos para formarlas y utilizarlas
CN104212822A (zh) 2007-01-30 2014-12-17 Bp法人北美有限公司 用于处理木质纤维素的酶、编码它们的核酸及其制备和应用方法
CA2679503A1 (en) 2007-03-01 2008-09-04 Verenium Corporation Nitrilases, nucleic acids encoding them and methods for making and using them
NZ601191A (en) 2007-10-03 2014-01-31 Verenium Corp Xylanases, nucleic acids encoding them and methods for making and using them
DK2238242T5 (en) 2008-01-03 2015-08-03 Basf Enzymes Llc -Transferases and oxidoreductases, nucleic acids encoding FOR THEM, AND PROCESSES FOR THE STILL LING AND USE THEREOF
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
CN109738224B (zh) 2013-06-21 2021-07-16 伯乐生命医学产品有限公司 具有流体收集管的微流体系统
EP3708256B1 (en) 2015-04-22 2022-11-30 Stilla Technologies Contact-less priming method for loading a solution in a microfluidic device and associated system
AU2016271049B9 (en) 2015-05-29 2019-07-18 Illumina, Inc. Sample carrier and assay system for conducting designated reactions
KR102379308B1 (ko) * 2015-11-04 2022-03-28 닛토덴코 가부시키가이샤 생체유체 샘플 분배 및/또는 분석을 위한 장치 및 시스템
CN108700609A (zh) * 2015-12-30 2018-10-23 生物辐射实验室股份有限公司 具有自动校准的液滴测定系统
AU2017223404B2 (en) 2016-02-22 2022-09-22 Biofire Defense, Llc Devices and methods for rapid PCR
JP6622147B2 (ja) * 2016-06-16 2019-12-18 株式会社エンプラス エマルション生成部材、およびこれを用いたサンプル採取管、ならびに採血管
KR102578419B1 (ko) * 2016-07-28 2023-09-14 바이오파이어 다이어그노스틱스, 엘엘씨 자체-구비형 핵산 처리
USD803173S1 (en) * 2016-09-07 2017-11-21 EMULATE, Inc. Perfusion module lid without pressure features
GB201617869D0 (en) * 2016-10-21 2016-12-07 Blacktrace Holdings Limited A microfluidic device
CN108148743A (zh) * 2016-12-06 2018-06-12 中山百慧生物科技有限公司 一种液滴数字pcr芯片及相应检测方法和检测系统
CN108148744A (zh) * 2016-12-06 2018-06-12 中山百慧生物科技有限公司 一种液滴数字pcr芯片及相应检测方法和检测系统
EP3579975A4 (en) * 2017-02-13 2021-03-24 Bio-rad Laboratories, Inc. SYSTEM, METHOD AND DEVICE FOR FORMING A SERIES OF EMULSIONS
CN106984370B (zh) * 2017-05-08 2019-09-20 北京旌准医疗科技有限公司 一种基于微流控芯片的自动进样系统
CN117143960A (zh) 2017-05-18 2023-12-01 10X基因组学有限公司 用于分选液滴和珠的方法和系统
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
GB2562762B (en) * 2017-05-24 2022-07-13 Univ Heriot Watt Microfluidic mixing
WO2019006190A1 (en) 2017-06-28 2019-01-03 Bio-Rad Laboratories, Inc. SYSTEM AND METHOD FOR DROPLELET DETECTION
KR101951405B1 (ko) * 2017-06-28 2019-05-08 주식회사 파나진 시료 시약 반응 분석용 키트, 이를 이용한 표적 핵산 검출 장치 및 방법
US10610865B2 (en) 2017-08-22 2020-04-07 10X Genomics, Inc. Droplet forming devices and system with differential surface properties
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
US11045805B2 (en) 2017-11-01 2021-06-29 Bio-Rad Laboratories, Inc. Microfluidic system and method for arranging objects
WO2019087176A1 (en) 2017-11-02 2019-05-09 Memed Diagnostics Ltd. Cartridge and system for analyzing body liquid
JP6967427B2 (ja) * 2017-11-06 2021-11-17 Nok株式会社 ガスケットおよび密封構造
EP3720603B1 (en) * 2017-12-06 2025-02-19 Samplix ApS A microfluidic device and a method for provision of emulsion droplets
KR101951670B1 (ko) 2017-12-13 2019-02-25 한국과학기술원 미세액적 내 유전자 검출을 위한 광학장치
EP3501651B1 (en) * 2017-12-22 2024-03-06 IMEC vzw Microfluidic routing
GB2572408B8 (en) * 2018-03-29 2022-06-22 Univ Heriot Watt Fluidic device
WO2019204187A1 (en) * 2018-04-15 2019-10-24 Optofluidic Bioassay, Llc Differential pressure assisted drainage system
KR101891968B1 (ko) * 2018-05-30 2018-08-27 티엔에스(주) 하이브리드 유전자칩
KR101891558B1 (ko) * 2018-06-15 2018-08-27 티엔에스(주) 하이브리드 유전자칩용 유전자 분석 장치
CN113841053B (zh) * 2018-08-09 2024-05-28 港大科桥有限公司 用于将流体样本自动化处理成微流体液滴以用于体外诊断的系统
CN108728347A (zh) * 2018-08-27 2018-11-02 中国农业科学院农业质量标准与检测技术研究所 一种基因检测装置
CN109351369B (zh) * 2018-10-23 2021-01-26 深圳市博瑞生物科技有限公司 微流控液滴生成芯片
CN109351368B (zh) * 2018-10-23 2021-04-30 深圳市博瑞生物科技有限公司 微流控芯片
CN113874114A (zh) * 2019-04-08 2021-12-31 技术研究及发展基金有限公司 纳升液滴阵列设备的多路复用阵列
JP7161624B2 (ja) * 2019-08-01 2022-10-26 日本ケミファ株式会社 試薬カートリッジ
WO2021077087A1 (en) * 2019-10-18 2021-04-22 Morningbird Media Corporation Three-dimensional printer with multihead extruder and compositions for use therein
CN111304060A (zh) * 2020-03-30 2020-06-19 西安天隆科技有限公司 一种微液滴生成芯片
EP4126319A1 (en) * 2020-04-01 2023-02-08 Merck Patent GmbH Emulsification device
CN111826273B (zh) * 2020-07-22 2023-03-21 上海逢伙泰企业管理有限公司 一种用于核酸检测的自动化全封闭微流控芯片
DE102020211697A1 (de) * 2020-09-18 2022-03-24 Robert Bosch Gesellschaft mit beschränkter Haftung Mikrofluidische Vorrichtung
US11465145B2 (en) * 2020-09-18 2022-10-11 Microgem International Plc Method and system for sample collection, storage, preparation and detection
US20250177973A1 (en) * 2022-02-25 2025-06-05 Redbud Labs, Inc. Sample tube connectors and methods for attaching a microfluidics device to a sample tube
CN114672412A (zh) * 2022-05-30 2022-06-28 季华实验室 一种微滴式数字聚合酶链反应芯片
EP4431185A1 (en) * 2023-03-17 2024-09-18 CSEM Centre Suisse d'Electronique et de Microtechnique SA - Recherche et Développement Liquid handling cover for a microfluidic device

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5942443A (en) 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US20020092767A1 (en) * 1997-09-19 2002-07-18 Aclara Biosciences, Inc. Multiple array microfluidic device units
US6902703B2 (en) * 1999-05-03 2005-06-07 Ljl Biosystems, Inc. Integrated sample-processing system
US20030215957A1 (en) * 1998-02-20 2003-11-20 Tony Lemmo Multi-channel dispensing system
ATE341002T1 (de) * 1999-02-16 2006-10-15 Applera Corp Vorrichtung zur handhabung von kügelchen
US8709153B2 (en) 1999-06-28 2014-04-29 California Institute Of Technology Microfludic protein crystallography techniques
CA2380897A1 (en) 1999-08-25 2001-03-01 Caliper Technologies Corporation Dilutions in high throughput systems with a single vacuum source
NL1017374C2 (nl) * 2001-02-15 2002-08-16 Univ Delft Tech Inrichting voor het uitvoeren van een reactie, alsmede een werkwijze voor het uitvoeren van een reactie in de inrichting.
WO2002072423A1 (en) * 2001-03-09 2002-09-19 Biomicro Systems, Inc. Microplate lid
US20020187557A1 (en) * 2001-06-07 2002-12-12 Hobbs Steven E. Systems and methods for introducing samples into microfluidic devices
US6877528B2 (en) * 2002-04-17 2005-04-12 Cytonome, Inc. Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
CA2512071A1 (en) 2002-12-30 2004-07-22 The Regents Of The University Of California Methods and apparatus for pathogen detection and analysis
KR20080010395A (ko) * 2005-04-26 2008-01-30 아비자 테크놀로지 리미티드 미세유체 구조물과 이를 제조하는 방법
US8257964B2 (en) 2006-01-04 2012-09-04 Cell ASIC Microwell cell-culture device and fabrication method
EP2144843B1 (en) * 2007-05-15 2019-10-23 Wako Pure Chemical Industries, Ltd. Method of controlling pressure in a microfluidic device
EP2717041B1 (en) 2007-06-11 2015-05-27 Wako Pure Chemical Industries, Ltd. Microchip large volume polymerase chain reaction (PCR) with integrated real-time capillary electrophoresis detection
GB2477053B (en) * 2008-09-23 2013-11-13 Quantalife Inc Droplet-based assay system
US9157550B2 (en) * 2009-01-05 2015-10-13 The Board Of Trustees Of The University Of Illinois Microfluidic systems and methods
NL2002862C2 (en) * 2009-05-08 2010-11-09 Friesland Brands Bv Microfluidic apparatus and method for generating a dispersion.
WO2011002957A2 (en) * 2009-07-01 2011-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
FR2951153A1 (fr) 2009-10-09 2011-04-15 Corning Inc Dispositif microfluidique
EP2550528B1 (en) * 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
EP2593228A4 (en) * 2010-07-15 2016-07-13 Indian Statistical Inst ARCHITECTURAL PLAN FOR DILUTION WITH REDUCED WASTE IN A CHIP LABORATORY BASED ON DIGITAL MICROFLUIDIC TECHNOLOGY
DE102010047384B4 (de) * 2010-10-02 2012-06-28 Karlsruher Institut für Technologie Vorrichtung und Verfahren zur Erzeugung oder zur Ablage eines Fluidstroms aus Fluidsegmenten und ihre Verwendung
EP3132844B1 (en) * 2010-11-01 2019-08-28 Bio-Rad Laboratories, Inc. System for forming emulsions
CN102389730A (zh) * 2011-08-23 2012-03-28 东南大学 一种双重乳液制备芯片
SG11201401810QA (en) 2011-09-28 2014-10-30 Harvard College Systems and methods for droplet production and/or fluidic manipulation
CN109738224B (zh) 2013-06-21 2021-07-16 伯乐生命医学产品有限公司 具有流体收集管的微流体系统

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