JP6416887B2 - 構造化照明を用いた組織試料の顕微鏡観察 - Google Patents

構造化照明を用いた組織試料の顕微鏡観察 Download PDF

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JP6416887B2
JP6416887B2 JP2016514083A JP2016514083A JP6416887B2 JP 6416887 B2 JP6416887 B2 JP 6416887B2 JP 2016514083 A JP2016514083 A JP 2016514083A JP 2016514083 A JP2016514083 A JP 2016514083A JP 6416887 B2 JP6416887 B2 JP 6416887B2
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light
image
tissue sample
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JP2016526185A5 (enExample
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クインシー ブラウン，ジョナサン
クインシー ブラウン，ジョナサン
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ジ アドミニストレーターズ オブ ザ テュレーン エデュケーショナル ファンド
ジ アドミニストレーターズ オブ ザ テュレーン エデュケーショナル ファンド
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Instruments for taking body samples for diagnostic purposes; Other methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determination; Throat striking implements
    • A61B10/02—Instruments for taking cell samples or for biopsy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F18/00—Pattern recognition
    • G06F18/20—Analysing
    • G06F18/24—Classification techniques
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V10/00—Arrangements for image or video recognition or understanding
    • G06V10/40—Extraction of image or video features
    • G06V10/42—Global feature extraction by analysis of the whole pattern, e.g. using frequency domain transformations or autocorrelation
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/56—Cameras or camera modules comprising electronic image sensors; Control thereof provided with illuminating means
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    • A61B90/00—Instruments, implements or accessories specially adapted for surgery or diagnosis and not covered by any of the groups A61B1/00 - A61B50/00, e.g. for luxation treatment or for protecting wound edges
    • A61B90/36—Image-producing devices or illumination devices not otherwise provided for
    • A61B2090/364—Correlation of different images or relation of image positions in respect to the body
    • A61B2090/366—Correlation of different images or relation of image positions in respect to the body using projection of images directly onto the body
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B90/00—Instruments, implements or accessories specially adapted for surgery or diagnosis and not covered by any of the groups A61B1/00 - A61B50/00, e.g. for luxation treatment or for protecting wound edges
    • A61B90/39—Markers, e.g. radio-opaque or breast lesions markers
    • A61B2090/3937—Visible markers
    • A61B2090/3941—Photoluminescent markers
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/302—Stain compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/063—Illuminating optical parts
    • G01N2201/0635—Structured illumination, e.g. with grating
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/067—Electro-optic, magneto-optic, acousto-optic elements
    • G01N2201/0675—SLM
    • G—PHYSICS
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G01N2201/129—Using chemometrical methods

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  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Multimedia (AREA)
  • Optics & Photonics (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Public Health (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Surgery (AREA)
  • Medical Informatics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Theoretical Computer Science (AREA)
  • Data Mining & Analysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biophysics (AREA)
  • Artificial Intelligence (AREA)
  • Signal Processing (AREA)
  • General Engineering & Computer Science (AREA)
  • Evolutionary Biology (AREA)
  • Evolutionary Computation (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2016514083A 2013-05-15 2014-05-15 構造化照明を用いた組織試料の顕微鏡観察 Expired - Fee Related JP6416887B2 (ja)

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US201361823817P 2013-05-15 2013-05-15
US61/823,817 2013-05-15
US201361919434P 2013-12-20 2013-12-20
US61/919,434 2013-12-20
PCT/US2014/038141 WO2014186544A1 (en) 2013-05-15 2014-05-15 Microscopy of a tissue sample using structured illumination

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JP2016526185A JP2016526185A (ja) 2016-09-01
JP2016526185A5 JP2016526185A5 (enExample) 2018-04-12
JP6416887B2 true JP6416887B2 (ja) 2018-10-31

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EP (1) EP2997353B1 (enExample)
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AU (1) AU2014265382B2 (enExample)
CA (1) CA2912401C (enExample)
DK (1) DK2997353T3 (enExample)
ES (1) ES2935506T3 (enExample)
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Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017075275A1 (en) * 2015-10-29 2017-05-04 The Board Of Trustees Of The Leland Stanford Junior University Methods and systems for imaging a biological sample
WO2017139649A2 (en) 2016-02-12 2017-08-17 Massachusetts Institute Of Technology Method and apparatus for imaging unsectioned tissue speciments
WO2017197491A1 (en) * 2016-05-19 2017-11-23 Huron Technologies International Inc. Spectrally-resolved scanning microscope
CN106291962B (zh) * 2016-09-19 2018-07-17 浙江大学 基于偏振光相位调制的结构光生成装置与方法
EP3538870B1 (en) * 2016-11-14 2024-09-04 Siemens Healthcare Diagnostics Inc. Methods and apparatus for characterizing a specimen using pattern illumination
CN106706577B (zh) * 2016-11-16 2019-08-16 深圳大学 一种光成像系统及方法
WO2018173121A1 (ja) 2017-03-21 2018-09-27 株式会社Ｐｒｅｆｅｒｒｅｄ Ｎｅｔｗｏｒｋｓ サーバ装置、学習済モデル提供プログラム、学習済モデル提供方法及び学習済モデル提供システム
US10401605B2 (en) * 2017-05-02 2019-09-03 SCREEN Holdings, Co., Ltd. Structured illumination in inverted light sheet microscopy
EP3467563A1 (de) * 2017-10-06 2019-04-10 Siemens Healthcare GmbH Mikroskopiervorrichtung
CN108120680B (zh) * 2017-12-19 2019-11-22 清华大学 基于光电特性先验的显微成像的杂散光去除方法及装置
TWI699816B (zh) * 2017-12-26 2020-07-21 雲象科技股份有限公司 自動化顯微鏡系統之控制方法、顯微鏡系統及電腦可讀取記錄媒體
JP6986452B2 (ja) * 2018-01-04 2021-12-22 浜松ホトニクス株式会社 蛍光測定装置および蛍光測定方法
WO2019148024A1 (en) 2018-01-26 2019-08-01 Park Jong Kang Systems and methods to reduce scattering in temporal focusing multiphoton microscopy
CA3095932A1 (en) 2018-04-05 2019-10-10 Instapath, Inc. Disposable biopsy staining kit and related methods and systems
US12181579B2 (en) 2018-07-16 2024-12-31 Cilag GmbH Intemational Controlling an emitter assembly pulse sequence
KR102130100B1 (ko) * 2018-09-07 2020-07-03 주식회사 더웨이브톡 광학 검출 시스템
WO2020066040A1 (ja) 2018-09-28 2020-04-02 オリンパス株式会社 顕微鏡システム、投影ユニット、及び、画像投影方法
EP4345776A3 (en) 2018-09-28 2024-06-19 Evident Corporation Microscope system, projection unit, and image projection method
CN112703440B (zh) 2018-09-28 2022-12-23 仪景通株式会社 显微镜系统
CN112714888B (zh) 2018-09-28 2023-02-17 仪景通株式会社 显微镜系统、投影单元以及图像投影方法
US20200284703A1 (en) * 2019-03-08 2020-09-10 Samantree Medical Sa Staining agent solutions for use in fluorescence microscopy
US12257013B2 (en) 2019-03-15 2025-03-25 Cilag Gmbh International Robotic surgical systems with mechanisms for scaling camera magnification according to proximity of surgical tool to tissue
US12618755B2 (en) 2019-03-20 2026-05-05 Dany Adams Methods and systems for assessing bioelectric patterns
WO2020210746A1 (en) * 2019-04-12 2020-10-15 Invenio Imaging, Inc. Imaging system for detection of intraoperative contrast agents in tissue
US11514575B2 (en) * 2019-10-01 2022-11-29 10X Genomics, Inc. Systems and methods for identifying morphological patterns in tissue samples
DE102019129932B4 (de) * 2019-11-06 2023-12-21 Technische Universität Braunschweig Optische Detektionseinrichtung und Verfahren zum Betreiben einer optischen Detektionseinrichtung
KR102304036B1 (ko) * 2019-11-27 2021-09-17 울산과학기술원 연속 블록면 광간섭 현미경 장치
TWI868262B (zh) * 2019-12-06 2025-01-01 美商伊路米納有限公司 提供參數估計的裝置和方法以及相關的處理器可讀取媒體
US12002571B2 (en) 2019-12-30 2024-06-04 Cilag Gmbh International Dynamic surgical visualization systems
US12053223B2 (en) 2019-12-30 2024-08-06 Cilag Gmbh International Adaptive surgical system control according to surgical smoke particulate characteristics
US12207881B2 (en) 2019-12-30 2025-01-28 Cilag Gmbh International Surgical systems correlating visualization data and powered surgical instrument data
US11896442B2 (en) 2019-12-30 2024-02-13 Cilag Gmbh International Surgical systems for proposing and corroborating organ portion removals
US11744667B2 (en) 2019-12-30 2023-09-05 Cilag Gmbh International Adaptive visualization by a surgical system
US12453592B2 (en) 2019-12-30 2025-10-28 Cilag Gmbh International Adaptive surgical system control according to surgical smoke cloud characteristics
US11284963B2 (en) 2019-12-30 2022-03-29 Cilag Gmbh International Method of using imaging devices in surgery
US11832996B2 (en) 2019-12-30 2023-12-05 Cilag Gmbh International Analyzing surgical trends by a surgical system
US11776144B2 (en) 2019-12-30 2023-10-03 Cilag Gmbh International System and method for determining, adjusting, and managing resection margin about a subject tissue
US11759283B2 (en) 2019-12-30 2023-09-19 Cilag Gmbh International Surgical systems for generating three dimensional constructs of anatomical organs and coupling identified anatomical structures thereto
WO2021252280A1 (en) * 2020-06-08 2021-12-16 The Regents Of The University Of California White dwarf: cross-polarized white light slide-free imaging
JP7621474B2 (ja) 2020-10-15 2025-01-24 アプライド マテリアルズ インコーポレイテッド 光学装置のためのシースルー計測システム、装置、及び方法
DE102020131047B4 (de) 2020-11-24 2024-08-22 Abberior Instruments Gmbh Verfahren und Vorrichtung zur Aufnahme nanoskopischer Bilder mehrfach gefärbter Proben
EP4274465A4 (en) * 2021-01-05 2024-10-23 Cytoveris Inc. MULTIMODAL MULTISPECTRAL IMAGING SYSTEM AND METHOD FOR CHARACTERIZING TISSUE TYPES IN BLADDER SAMPLES
CN113568294B (zh) * 2021-07-16 2022-07-12 西安交通大学 一种全息光镊融合结构光照明显微系统和方法

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6671540B1 (en) * 1990-08-10 2003-12-30 Daryl W. Hochman Methods and systems for detecting abnormal tissue using spectroscopic techniques
JP2000095758A (ja) * 1998-09-18 2000-04-04 Schering Ag 近赤外蛍光造影剤および蛍光造影方法
US7139598B2 (en) * 2002-04-04 2006-11-21 Veralight, Inc. Determination of a measure of a glycation end-product or disease state using tissue fluorescence
JP4700001B2 (ja) * 2003-09-19 2011-06-15 ザ ジェネラル ホスピタル コーポレイション 蛍光偏極撮像方法
US20070016078A1 (en) * 2004-12-06 2007-01-18 Hoyt Clifford C Systems and methods for in-vivo optical imaging and measurement
US8504140B2 (en) 2008-04-08 2013-08-06 Bruker Biospin Corporation Apparatus and method for fluorescence imaging and tomography using spatially structured illumination
JP2007199572A (ja) * 2006-01-30 2007-08-09 Nikon Corp 顕微鏡装置
US9080977B2 (en) * 2006-10-23 2015-07-14 Xenogen Corporation Apparatus and methods for fluorescence guided surgery
EP2110697B1 (en) * 2008-04-17 2016-02-10 Ruprecht-Karls-Universität Heidelberg Wave field microscope with sub-wavelength resolution and methods for processing microscopic images to detect objects with sub-wavelength dimensions
US8406859B2 (en) * 2008-08-10 2013-03-26 Board Of Regents, The University Of Texas System Digital light processing hyperspectral imaging apparatus
DE102008049878A1 (de) 2008-09-30 2010-04-01 Carl Zeiss Microlmaging Gmbh Verbesserte Verfahren und Vorrichtungen für die Mikroskopie mit strukturierter Beleuchtung
US20110224574A1 (en) 2008-11-13 2011-09-15 Sadler John W Methods and systems for tissue processing and imaging
JP2010151566A (ja) * 2008-12-25 2010-07-08 Hitachi High-Technologies Corp 粒子画像解析方法及び装置
WO2010132990A1 (en) * 2009-05-22 2010-11-25 British Columbia Cancer Agency Branch Selective excitation light fluorescence imaging methods and apparatus
JP2013513823A (ja) * 2009-12-09 2013-04-22 アプライド プレシジョン インコーポレイテッド 高速の３次元構造化照明による顕微鏡撮像の方法およびシステム
EP2629133A4 (en) * 2010-10-14 2017-12-13 Nikon Corporation Structured illumination device, structured illumination microscope device, and surface shape measurement device
JP5655608B2 (ja) 2011-02-08 2015-01-21 株式会社ニコン 観察装置及び画像補正プログラム
US9867525B2 (en) * 2011-04-01 2018-01-16 Nanyang Technological University High sensitivity temporal focusing widefield multiphoton endoscope capable of deep imaging
US20120307247A1 (en) * 2011-05-31 2012-12-06 Nanyang Technological University Fluorescence Microscopy Method And System
JP5834584B2 (ja) 2011-07-25 2015-12-24 ソニー株式会社 情報処理装置、情報処理方法、プログラム及び蛍光スペクトルの強度補正方法
WO2013021615A1 (ja) 2011-08-11 2013-02-14 株式会社ニコン 構造化照明装置及び構造化照明方法、並びに構造化照明顕微鏡装置
US9254103B2 (en) * 2011-08-15 2016-02-09 The Trustees Of Dartmouth College Operative microscope having diffuse optical imaging system with tomographic image reconstruction and superposition in field of view
US9599805B2 (en) 2011-10-19 2017-03-21 National Synchrotron Radiation Research Center Optical imaging system using structured illumination

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