JP6340359B2 - 少なくとも１種の２−エチルヘキシルエステル、並びにエチルアルコールおよびイソプロピルアルコールを含む組成物 - Google Patents
少なくとも１種の２−エチルヘキシルエステル、並びにエチルアルコールおよびイソプロピルアルコールを含む組成物 Download PDFInfo
- Publication number
- JP6340359B2 JP6340359B2 JP2015502560A JP2015502560A JP6340359B2 JP 6340359 B2 JP6340359 B2 JP 6340359B2 JP 2015502560 A JP2015502560 A JP 2015502560A JP 2015502560 A JP2015502560 A JP 2015502560A JP 6340359 B2 JP6340359 B2 JP 6340359B2
- Authority
- JP
- Japan
- Prior art keywords
- ethylhexyl
- alcohol
- composition
- concentration
- ethyl alcohol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims description 71
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 title claims description 57
- 239000000203 mixture Substances 0.000 title claims description 50
- 235000019441 ethanol Nutrition 0.000 title claims description 47
- NPSJHQMIVNJLNN-UHFFFAOYSA-N 2-ethylhexyl 4-nitrobenzoate Chemical compound CCCCC(CC)COC(=O)C1=CC=C([N+]([O-])=O)C=C1 NPSJHQMIVNJLNN-UHFFFAOYSA-N 0.000 title claims description 17
- 239000004808 2-ethylhexylester Substances 0.000 title claims description 17
- 238000012545 processing Methods 0.000 claims description 40
- 239000000523 sample Substances 0.000 claims description 30
- 239000012472 biological sample Substances 0.000 claims description 18
- UADWUILHKRXHMM-UHFFFAOYSA-N 2-ethylhexyl benzoate Chemical compound CCCCC(CC)COC(=O)C1=CC=CC=C1 UADWUILHKRXHMM-UHFFFAOYSA-N 0.000 claims description 15
- 229940106004 2-ethylhexyl benzoate Drugs 0.000 claims description 15
- UADWUILHKRXHMM-ZDUSSCGKSA-N benzoflex 181 Natural products CCCC[C@H](CC)COC(=O)C1=CC=CC=C1 UADWUILHKRXHMM-ZDUSSCGKSA-N 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 11
- -1 2-ethylhexyl Chemical group 0.000 claims description 9
- 230000018044 dehydration Effects 0.000 claims description 9
- 238000006297 dehydration reaction Methods 0.000 claims description 9
- 238000011888 autopsy Methods 0.000 claims description 7
- WOYWLLHHWAMFCB-UHFFFAOYSA-N 2-ethylhexyl acetate Chemical compound CCCCC(CC)COC(C)=O WOYWLLHHWAMFCB-UHFFFAOYSA-N 0.000 claims description 6
- SFAAOBGYWOUHLU-UHFFFAOYSA-N 2-ethylhexyl hexadecanoate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(CC)CCCC SFAAOBGYWOUHLU-UHFFFAOYSA-N 0.000 claims description 6
- OPJWPPVYCOPDCM-UHFFFAOYSA-N 2-ethylhexyl octadecanoate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(CC)CCCC OPJWPPVYCOPDCM-UHFFFAOYSA-N 0.000 claims description 6
- 229940071160 cocoate Drugs 0.000 claims description 6
- 230000002380 cytological effect Effects 0.000 claims description 6
- 238000004458 analytical method Methods 0.000 claims description 3
- 238000002991 immunohistochemical analysis Methods 0.000 claims description 3
- 102100032218 Cytokine-inducible SH2-containing protein Human genes 0.000 claims description 2
- 101000943420 Homo sapiens Cytokine-inducible SH2-containing protein Proteins 0.000 claims description 2
- 150000001298 alcohols Chemical class 0.000 claims description 2
- 238000002509 fluorescent in situ hybridization Methods 0.000 claims description 2
- 238000007901 in situ hybridization Methods 0.000 claims description 2
- 238000004452 microanalysis Methods 0.000 claims description 2
- 238000001823 molecular biology technique Methods 0.000 claims description 2
- 230000003796 beauty Effects 0.000 claims 1
- 239000012188 paraffin wax Substances 0.000 description 21
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 17
- 239000003153 chemical reaction reagent Substances 0.000 description 16
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 12
- TVMXDCGIABBOFY-UHFFFAOYSA-N octane Chemical compound CCCCCCCC TVMXDCGIABBOFY-UHFFFAOYSA-N 0.000 description 9
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 208000005156 Dehydration Diseases 0.000 description 6
- 238000001574 biopsy Methods 0.000 description 6
- 239000002904 solvent Substances 0.000 description 6
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 5
- 238000010186 staining Methods 0.000 description 5
- XMGQYMWWDOXHJM-JTQLQIEISA-N (+)-α-limonene Chemical compound CC(=C)[C@@H]1CCC(C)=CC1 XMGQYMWWDOXHJM-JTQLQIEISA-N 0.000 description 4
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 4
- 230000002055 immunohistochemical effect Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 210000003484 anatomy Anatomy 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 230000000877 morphologic effect Effects 0.000 description 3
- 238000004445 quantitative analysis Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- UWNADWZGEHDQAB-UHFFFAOYSA-N 2,5-dimethylhexane Chemical group CC(C)CCC(C)C UWNADWZGEHDQAB-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- XTDYIOOONNVFMA-UHFFFAOYSA-N dimethyl pentanedioate Chemical compound COC(=O)CCCC(=O)OC XTDYIOOONNVFMA-UHFFFAOYSA-N 0.000 description 2
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 239000003021 water soluble solvent Substances 0.000 description 2
- 239000008096 xylene Substances 0.000 description 2
- RDOFJDLLWVCMRU-UHFFFAOYSA-N Diisobutyl adipate Chemical compound CC(C)COC(=O)CCCCC(=O)OCC(C)C RDOFJDLLWVCMRU-UHFFFAOYSA-N 0.000 description 1
- UDSFAEKRVUSQDD-UHFFFAOYSA-N Dimethyl adipate Chemical compound COC(=O)CCCCC(=O)OC UDSFAEKRVUSQDD-UHFFFAOYSA-N 0.000 description 1
- MUXOBHXGJLMRAB-UHFFFAOYSA-N Dimethyl succinate Chemical compound COC(=O)CCC(=O)OC MUXOBHXGJLMRAB-UHFFFAOYSA-N 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- WNLRTRBMVRJNCN-UHFFFAOYSA-L adipate(2-) Chemical compound [O-]C(=O)CCCCC([O-])=O WNLRTRBMVRJNCN-UHFFFAOYSA-L 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000003124 biologic agent Substances 0.000 description 1
- QCOAPBRVQHMEPF-UHFFFAOYSA-N bis(2-methylpropyl) butanedioate Chemical compound CC(C)COC(=O)CCC(=O)OCC(C)C QCOAPBRVQHMEPF-UHFFFAOYSA-N 0.000 description 1
- UFWRCRCDRAUAAO-UHFFFAOYSA-N bis(2-methylpropyl) pentanedioate Chemical compound CC(C)COC(=O)CCCC(=O)OCC(C)C UFWRCRCDRAUAAO-UHFFFAOYSA-N 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000010779 crude oil Substances 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 229940031769 diisobutyl adipate Drugs 0.000 description 1
- 238000001839 endoscopy Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 125000005909 ethyl alcohol group Chemical group 0.000 description 1
- 239000000834 fixative Substances 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- 230000001744 histochemical effect Effects 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000001073 sample cooling Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
- G01N2001/307—Fixative compositions non-toxic, no Hg, no formaldehyde
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Steroid Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT000004A ITCA20120004A1 (it) | 2012-03-30 | 2012-03-30 | Unico reattivo disidratante e diafanizzante per istologia e citologia non nocivo e non tossico, biodegradabile 88%, a bassa volatilita' |
| ITCA2012A000004 | 2012-03-30 | ||
| ITRM2012A000583 | 2012-11-21 | ||
| IT000583A ITRM20120583A1 (it) | 2012-03-30 | 2012-11-21 | Composizione per la processazione di campioni istologici, autoptici, citologici. |
| PCT/IT2013/000088 WO2013144986A2 (en) | 2012-03-30 | 2013-03-25 | Composition for processing histological, postmortem, cytological samples |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2015511722A JP2015511722A (ja) | 2015-04-20 |
| JP6340359B2 true JP6340359B2 (ja) | 2018-06-06 |
Family
ID=45955292
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015502560A Expired - Fee Related JP6340359B2 (ja) | 2012-03-30 | 2013-03-25 | 少なくとも１種の２−エチルヘキシルエステル、並びにエチルアルコールおよびイソプロピルアルコールを含む組成物 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US10139320B2 (it) |
| EP (1) | EP2831560B1 (it) |
| JP (1) | JP6340359B2 (it) |
| CN (1) | CN104797921B (it) |
| CA (1) | CA2905652C (it) |
| ES (1) | ES2688322T3 (it) |
| IT (2) | ITCA20120004A1 (it) |
| WO (1) | WO2013144986A2 (it) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017041005A1 (en) * | 2015-09-03 | 2017-03-09 | Abbott Molecular Inc. | Hybridization buffers comprising an alkyl diester |
| US10107727B2 (en) | 2015-12-30 | 2018-10-23 | Sakura Finetek U.S.A., Inc. | Tissue processing reagent |
| US9835527B2 (en) | 2015-12-30 | 2017-12-05 | Sakura Finetek U.S.A., Inc. | Tissue processing reagent |
| US10457981B2 (en) | 2016-01-08 | 2019-10-29 | Abbott Molecular Inc. | Hybridization buffers |
| CN111982637B (zh) * | 2020-08-06 | 2023-08-18 | 西南大学 | 一种环保的石蜡切片透明脱蜡试剂 |
| US20230393038A1 (en) * | 2020-10-23 | 2023-12-07 | Diapath S.P.A. | Composition for the treatment of biological, cytological, histological and autopsical samples |
| IT202000025159A1 (it) | 2020-10-23 | 2022-04-23 | Diapath S P A | Composizione per il trattamento di campioni biologici, citologici, istologici e autoptici. |
| IT202200014608A1 (it) | 2022-07-12 | 2024-01-12 | Diapath S P A | Composizione migliorata per il trattamento di campioni biologici, citologici, istologici e autoptici. |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4263278A (en) * | 1979-07-10 | 1981-04-21 | Saurino Vincent R | Mortuary composition |
| DE3150988A1 (de) | 1980-12-30 | 1982-08-05 | Institut Français du Pétrole, 92502 Rueil-Malmaison, Hauts-de-Seine | Brennbare kompositionen, die alkohole und fettsaeureester enthalten und insbesondere als dieseltreibstoffe brauchbar sind |
| US4425328A (en) * | 1981-12-21 | 1984-01-10 | American Cyanamid Company | Solid antiperspirant stick composition |
| EP0653617A3 (en) | 1993-11-12 | 1997-10-22 | Shino Junko | Fat-soluble substitute for the substitution of a drainage agent for the manufacture of a tissue preparation. |
| JP2602789B2 (ja) * | 1993-11-12 | 1997-04-23 | 篠 純子 | 組織標本作製用の脱水剤を置換するための脂溶性置換剤及び前記脂溶性置換剤を用いた組織標本作製方法 |
| DK142193D0 (da) * | 1993-12-21 | 1993-12-21 | Esti Chem A S | Anvendelse af fedtsyreestere |
| ES2119535T3 (es) | 1996-08-02 | 1998-10-01 | Milestone Srl | Procedimiento para procesar muestras organicas. |
| FR2787712B1 (fr) * | 1998-12-24 | 2002-08-30 | Sanofi Sa | Composition cosmetique pulverisable et matrice utilisable dans cette composition pour une administration dermique |
| EP1276372B1 (en) * | 2000-04-28 | 2005-10-19 | Ecolab Inc. | Antimicrobial composition |
| DE10221335B4 (de) | 2002-05-10 | 2006-05-24 | Braun Gmbh | Verwendung einer Reinigungsflüssigkeit für elektrische Rasierer |
| ES2329355T3 (es) | 2002-05-28 | 2009-11-25 | Diapath S.P.A. | Composicion para la preparacion de muestras histologicas, citologicas y de autopsia. |
| NZ544311A (en) * | 2003-06-30 | 2008-12-24 | Groningen Acad Ziekenhuis | Method for histoprocessing |
| GB0426308D0 (en) * | 2004-12-01 | 2004-12-29 | Byotrol Llc | Preservative and embalming fluids |
| DE102005025495A1 (de) * | 2005-06-01 | 2006-12-14 | Henkel Kgaa | Ölhaltige desodorierende Aerosolzusammensetzungen mit hautkühlenden Wirkstoffen |
| CA2835365A1 (en) | 2006-01-13 | 2007-07-26 | Ventana Medical Systems, Inc. | Biological sample processing composition and method |
| WO2008112993A1 (en) * | 2007-03-15 | 2008-09-18 | Ventana Medical Systems, Inc. | Stabilized hematoxylin |
| ATE509095T1 (de) * | 2008-05-28 | 2011-05-15 | Symrise Ag | Menschliches ex-vivo-hautmodell |
| DE102009044948A1 (de) * | 2009-09-24 | 2011-03-31 | Henkel Ag & Co. Kgaa | Mittel zur Behandlung keratinischer Fasern enthaltend Dimethylsilanol Hyaluronate und Glycerin |
| DE102011013341A1 (de) * | 2011-03-08 | 2011-12-08 | Clariant International Ltd. | Polymere auf Basis von Sulfonsäuren, Amiden und speziellen Vernetzern |
| US20130251663A1 (en) * | 2012-03-23 | 2013-09-26 | Greenblendz, Inc. | Tissue Preservation Fluid |
-
2012
- 2012-03-30 IT IT000004A patent/ITCA20120004A1/it unknown
- 2012-11-21 IT IT000583A patent/ITRM20120583A1/it unknown
-
2013
- 2013-03-25 US US14/388,754 patent/US10139320B2/en active Active
- 2013-03-25 CN CN201380030196.2A patent/CN104797921B/zh not_active Expired - Fee Related
- 2013-03-25 EP EP13722583.5A patent/EP2831560B1/en active Active
- 2013-03-25 JP JP2015502560A patent/JP6340359B2/ja not_active Expired - Fee Related
- 2013-03-25 ES ES13722583.5T patent/ES2688322T3/es active Active
- 2013-03-25 WO PCT/IT2013/000088 patent/WO2013144986A2/en not_active Ceased
- 2013-03-25 CA CA2905652A patent/CA2905652C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ES2688322T3 (es) | 2018-10-31 |
| JP2015511722A (ja) | 2015-04-20 |
| ITCA20120004A1 (it) | 2012-06-29 |
| ITRM20120583A1 (it) | 2013-10-01 |
| CA2905652A1 (en) | 2013-10-03 |
| WO2013144986A2 (en) | 2013-10-03 |
| CN104797921B (zh) | 2018-04-03 |
| EP2831560B1 (en) | 2018-06-27 |
| US10139320B2 (en) | 2018-11-27 |
| WO2013144986A3 (en) | 2013-12-27 |
| EP2831560A2 (en) | 2015-02-04 |
| US20150050652A1 (en) | 2015-02-19 |
| CN104797921A (zh) | 2015-07-22 |
| CA2905652C (en) | 2020-07-28 |
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