JP6298404B2 - 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 - Google Patents

膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 Download PDF

Info

Publication number
JP6298404B2
JP6298404B2 JP2014522152A JP2014522152A JP6298404B2 JP 6298404 B2 JP6298404 B2 JP 6298404B2 JP 2014522152 A JP2014522152 A JP 2014522152A JP 2014522152 A JP2014522152 A JP 2014522152A JP 6298404 B2 JP6298404 B2 JP 6298404B2
Authority
JP
Japan
Prior art keywords
polynucleotide
target polynucleotide
pore
strand
stranded
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2014522152A
Other languages
English (en)
Japanese (ja)
Other versions
JP2014531196A (ja
JP2014531196A5 (https=
Inventor
ブラウン，クライブ
クラーク，ジェームス
ホール，グラハム
ハーパー，ギャビン
ヘロン，アンドリュー
ホワイト，ジェームス
Original Assignee
オックスフォード ナノポール テクノロジーズ リミテッド
オックスフォード ナノポール テクノロジーズ リミテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by オックスフォード ナノポール テクノロジーズ リミテッド, オックスフォード ナノポール テクノロジーズ リミテッド filed Critical オックスフォード ナノポール テクノロジーズ リミテッド
Publication of JP2014531196A publication Critical patent/JP2014531196A/ja
Publication of JP2014531196A5 publication Critical patent/JP2014531196A5/ja
Application granted granted Critical
Publication of JP6298404B2 publication Critical patent/JP6298404B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
JP2014522152A 2011-07-25 2012-07-25 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 Active JP6298404B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161511436P 2011-07-25 2011-07-25
US61/511,436 2011-07-25
PCT/GB2012/051786 WO2013014451A1 (en) 2011-07-25 2012-07-25 Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores

Publications (3)

Publication Number Publication Date
JP2014531196A JP2014531196A (ja) 2014-11-27
JP2014531196A5 JP2014531196A5 (https=) 2015-09-10
JP6298404B2 true JP6298404B2 (ja) 2018-03-20

Family

ID=46763140

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014522152A Active JP6298404B2 (ja) 2011-07-25 2012-07-25 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法

Country Status (10)

Country Link
US (7) US9957560B2 (https=)
EP (1) EP2737084B1 (https=)
JP (1) JP6298404B2 (https=)
KR (1) KR20140050067A (https=)
CN (1) CN103827320B (https=)
AU (1) AU2012288629B2 (https=)
BR (1) BR112014001699A2 (https=)
CA (1) CA2843136C (https=)
IN (1) IN2014DN00221A (https=)
WO (1) WO2013014451A1 (https=)

Families Citing this family (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3092369C (en) 2008-09-22 2025-09-23 University Of Washington MSP NANOPORES AND ASSOCIATED PROCESSES
JP5843614B2 (ja) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
SG10201604316WA (en) 2011-05-27 2016-07-28 Oxford Nanopore Tech Ltd Coupling method
AU2012288629B2 (en) 2011-07-25 2017-02-02 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
EP3663412B1 (en) 2011-09-23 2022-03-09 Oxford Nanopore Technologies PLC Analysis of a polymer comprising polymer units by means of translocation through a nanopore
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
US9617591B2 (en) 2011-12-29 2017-04-11 Oxford Nanopore Technologies Ltd. Method for characterising a polynucleotide by using a XPD helicase
BR112014016112B8 (pt) 2011-12-29 2022-12-27 Oxford Nanopore Tech Ltd Método e aparelho para caracterizar um polinucleotídeo alvo, kit, e, uso de uma helicase
US9238836B2 (en) 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
WO2013121201A1 (en) 2012-02-15 2013-08-22 Oxford Nanopore Technologies Limited Aptamer method
EP3540077B1 (en) * 2012-02-16 2021-01-27 Genia Technologies, Inc. Methods for creating bilayers for use with nanopore sensors
BR112014020211A2 (pt) 2012-02-16 2017-07-04 Oxford Nanopore Tech Ltd métodos para analisar uma série ordenada por tempo de medições de um polímero, para estimar a presença, ausência ou quantidade de um polímero alvo, e para determinar uma alteração em um polímero, programa de computador, e, dispositivos de análise e de diagnóstico
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
US9116118B2 (en) 2012-06-08 2015-08-25 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
EP2875128B8 (en) 2012-07-19 2020-06-24 Oxford Nanopore Technologies Limited Modified helicases
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
EP2895618B1 (en) 2012-09-14 2017-07-26 Oxford Nanopore Technologies Limited Sample preparation method
EP2917366B1 (en) 2012-11-06 2017-08-02 Oxford Nanopore Technologies Limited Quadruplex method
EP2917732B1 (en) * 2012-11-09 2016-09-07 Stratos Genomics Inc. Concentrating a target molecule for sensing by a nanopore
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
KR20150119353A (ko) * 2013-02-20 2015-10-23 이브 바이오메디컬, 인크. 나노구조-기반 핵산 서열분석을 위한 방법 및 조성물
KR102168813B1 (ko) * 2013-03-08 2020-10-22 옥스포드 나노포어 테크놀로지즈 리미티드 효소 정지 방법
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CN118256603A (zh) 2013-10-18 2024-06-28 牛津纳米孔科技公开有限公司 经修饰的酶
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
JP6749243B2 (ja) * 2014-01-22 2020-09-02 オックスフォード ナノポール テクノロジーズ リミテッド １つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法
ES3035698T3 (en) 2014-02-19 2025-09-08 Univ Washington Nanopore-based analysis of protein characteristics
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
EP3126515B1 (en) * 2014-04-04 2018-08-29 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
WO2016009180A1 (en) 2014-07-14 2016-01-21 Isis Innovation Limited Measurement of analytes with membrane channel molecules, and bilayer arrays
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
US10421998B2 (en) 2014-09-29 2019-09-24 The Regents Of The University Of California Nanopore sequencing of polynucleotides with multiple passes
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
EP3971300B1 (en) 2014-10-16 2024-06-05 Oxford Nanopore Technologies plc Sorting of polymers
KR102457147B1 (ko) 2014-10-17 2022-10-19 옥스포드 나노포어 테크놀로지즈 피엘씨 나노 세공 rna 특징 규명을 위한 방법
GB201418469D0 (en) * 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
WO2016077313A1 (en) 2014-11-11 2016-05-19 Bgi Shenzhen Multi-pass sequencing
EP3253889A4 (en) * 2015-02-02 2018-09-26 Two Pore Guys, Inc. Nanopore detection of target polynucleotides from sample background
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
CN107208162B (zh) 2015-02-26 2020-12-29 株式会社日立高新技术 核酸分子的构建方法
US11169138B2 (en) 2015-04-14 2021-11-09 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
CA2995422A1 (en) * 2015-08-12 2017-02-16 The Chinese University Of Hong Kong Single-molecule sequencing of plasma dna
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
KR102837999B1 (ko) 2015-12-08 2025-07-24 카트호리이케 유니버시타이트 로이펜 변형된 나노세공, 그를 포함하는 조성물, 및 그의 용도
EP3387108B1 (en) * 2015-12-08 2022-05-25 Quantapore, Inc. Method of translocating nucleic acids through nanopores
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
WO2017174990A1 (en) 2016-04-06 2017-10-12 Oxford Nanopore Technologies Limited Mutant pore
EP4063521B1 (en) 2016-05-25 2024-02-21 Oxford Nanopore Technologies PLC Method of nanopore sequencing
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
EP3464612B8 (en) * 2016-05-31 2024-12-11 Switchback Systems, Inc. Two-color nanopore sequencing
US10577653B2 (en) 2016-06-27 2020-03-03 Roche Sequencing Solutions, Inc. Counteracting osmotic imbalance in a sequencing cell
WO2018001925A1 (en) 2016-06-27 2018-01-04 F. Hoffmann-La Roche Ag Osmotic imbalance methods for bilayer formation
GB201612458D0 (en) * 2016-07-14 2016-08-31 Howorka Stefan And Pugh Genevieve Membrane spanning DNA nanopores for molecular transport
AU2017312746A1 (en) 2016-08-19 2019-01-31 Quantapore, Inc. Optically-based nanopore sequencing using quenching agents
GB201617886D0 (en) * 2016-10-21 2016-12-07 Oxford Nanopore Technologies Limited Method
GB201620450D0 (en) * 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
JP7048609B2 (ja) 2016-12-09 2022-04-05 ボリアル ジェノミクス， インコーポレイテッド 連結型ライゲーション
CN110267974A (zh) 2017-02-10 2019-09-20 牛津纳米孔技术公司 修饰的纳米孔、包含其的组合物及其用途
GB201707138D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Machine learning analysis of nanopore measurements
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CA3067993C (en) 2017-06-21 2024-02-27 Ontera Inc. Dual pore-control and sensor device
WO2019002893A1 (en) * 2017-06-30 2019-01-03 Vib Vzw NEW PROTEIN PORES
AU2018353924A1 (en) 2017-12-29 2019-07-18 Clear Labs, Inc. Automated priming and library loading device
WO2019183640A1 (en) * 2018-03-23 2019-09-26 Board Of Regents, The University Of Texas System Efficient sequencing of dsdna with extremely low level of errors
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
CN112639983B (zh) 2018-06-29 2024-06-04 豪夫迈·罗氏有限公司 微卫星不稳定性检测
WO2020039261A1 (en) 2018-08-23 2020-02-27 Boreal Genomics, Inc. Linked target capture and ligation
GB201814369D0 (en) 2018-09-04 2018-10-17 Oxford Nanopore Tech Ltd Method for determining a polymersequence
EP3650558A1 (en) * 2018-11-07 2020-05-13 Siemens Healthcare GmbH Liquid sample workflow for nanopore sequencing
EP3884064A4 (en) * 2018-11-19 2022-08-10 The Regents of The University of California METHODS FOR DETECTING AND SEQUENCING A TARGET NUCLEIC ACID
GB201819378D0 (en) 2018-11-28 2019-01-09 Oxford Nanopore Tech Ltd Analysis of nanopore signal using a machine-learning technique
WO2020141464A1 (en) * 2019-01-03 2020-07-09 Boreal Genomics, Inc. Linked target capture
WO2020168286A1 (en) 2019-02-14 2020-08-20 University Of Washington Systems and methods for improved nanopore-based analysis of nucleic acids
US11860123B2 (en) 2019-04-16 2024-01-02 The Board Of Trustees Of The University Of Illinois Classification of epigenetic biomarkers and/or DNA conformational superstructures via use of atomically thin nanopores
GB201917060D0 (en) * 2019-11-22 2020-01-08 Oxford Nanopore Tech Ltd Method
AU2021230282A1 (en) 2020-03-03 2022-09-22 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
US20230295712A1 (en) 2020-06-18 2023-09-21 Oxford Nanopore Technologies Plc A method of selectively characterising a polynucleotide using a detector
WO2022023753A1 (en) 2020-07-30 2022-02-03 Cambridge Epigenetix Limited Compositions and methods for nucleic acid analysis
US11248265B1 (en) 2020-11-19 2022-02-15 Clear Labs, Inc Systems and processes for distinguishing pathogenic and non-pathogenic sequences from specimens
CN115029424B (zh) * 2021-03-03 2025-05-16 郑州思昆生物工程有限公司 一种固定化模板多核苷酸配对末端测序方法
CN113462764B (zh) * 2021-09-01 2021-12-17 北京齐碳科技有限公司 用于表征双链靶多核苷酸的类发夹衔接子、构建体和方法
CN114134142B (zh) * 2021-11-23 2024-05-28 成都齐碳科技有限公司 衔接体、复合物、单链分子、试剂盒、方法和应用
CN114262735B (zh) * 2021-12-15 2025-09-16 成都齐碳科技有限公司 用于表征多核苷酸的衔接体及其用途
CN114457145B (zh) * 2022-01-29 2023-08-11 成都齐碳科技有限公司 用于表征靶多核苷酸测序的接头、构建体、方法和应用
IL320502A (en) 2022-10-28 2025-06-01 Univ Groningen Nanopore-based protein efflux
WO2024124567A1 (zh) * 2022-12-16 2024-06-20 深圳华大生命科学研究院 纳米孔测序方法和试剂盒
CN120265789A (zh) * 2022-12-29 2025-07-04 深圳华大生命科学研究院 测序接头、测序接头复合物、靶核酸序列多次纳米孔测序的方法
GB202414276D0 (en) 2024-09-27 2024-11-13 Oxford Nanopore Tech Plc Method

Family Cites Families (196)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI82266C (fi) 1982-10-19 1991-02-11 Cetus Corp Foerfarande foer framstaellning av il-2 -mutein.
GB8924338D0 (en) 1989-10-28 1989-12-13 Atomic Energy Authority Uk Electrodes
US5215899A (en) 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription
US5424413A (en) 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
FR2703693B1 (fr) 1993-04-06 1995-07-13 Pasteur Institut Procédé rapide de détermination d'une séquence d'ADN et application au séquençage et au diagnostic.
US5714320A (en) 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
EP0753071A1 (en) 1993-04-28 1997-01-15 Worcester Foundation For Experimental Biology Cell-targeted lytic pore-forming agents
US5777078A (en) 1993-04-28 1998-07-07 Worcester Foundation For Experimental Biology Triggered pore-forming agents
DE4320201A1 (de) 1993-06-18 1995-01-12 Asta Medica Ag Verwendung von Cetrorelix und weiteren Nona- und Dekapeptiden zur Herstellung eines Arzneimittels zur Bekämpfung von Aids und zur Wachstumsstimulation
US7569341B2 (en) 1994-01-31 2009-08-04 Trustees Of Boston University Nucleic acid directed immobilization arrays and methods of assembly
US5561043A (en) 1994-01-31 1996-10-01 Trustees Of Boston University Self-assembling multimeric nucleic acid constructs
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6395887B1 (en) 1995-08-01 2002-05-28 Yale University Analysis of gene expression by display of 3'-end fragments of CDNAS
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
DE19648625A1 (de) 1996-11-13 1998-05-14 Soft Gene Gmbh Mikroprojektil für das Einbringen von Substanzen in Zellen durch ballistischen Transfer
WO1999005167A1 (en) 1997-07-25 1999-02-04 University Of Massachusetts Designed protein pores as components for biosensors
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6127166A (en) 1997-11-03 2000-10-03 Bayley; Hagan Molluscan ligament polypeptides and genes encoding them
JPH11137260A (ja) 1997-11-06 1999-05-25 Soyaku Gijutsu Kenkyusho:Kk 抗インフルエンザウイルス環状ダンベル型ｒｎａ−ｄｎａキメラ化合物及び抗インフルエンザウイルス剤
US6123819A (en) 1997-11-12 2000-09-26 Protiveris, Inc. Nanoelectrode arrays
DE19826758C1 (de) 1998-06-15 1999-10-21 Soft Gene Gmbh Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6787308B2 (en) * 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6235502B1 (en) 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
WO2000034527A2 (en) 1998-12-11 2000-06-15 The Regents Of The University Of California Targeted molecular bar codes
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
JP2003502166A (ja) * 1999-06-22 2003-01-21 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 固体状態の次元的特徴の制御
WO2001002425A2 (en) 1999-06-29 2001-01-11 University Health Network Peptide conjugates for the stabilization of membrane proteins and interactions with biological membranes
JP2003527087A (ja) 1999-08-13 2003-09-16 イェール・ユニバーシティ バイナリーコード化配列タグ
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US6682649B1 (en) 1999-10-01 2004-01-27 Sophion Bioscience A/S Substrate and a method for determining and/or monitoring electrophysiological properties of ion channels
WO2001040516A2 (en) 1999-12-02 2001-06-07 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
WO2001059453A2 (en) 2000-02-11 2001-08-16 The Texas A & M University System Biosensor compositions and methods of use
US20020132978A1 (en) 2000-03-21 2002-09-19 Hans-Peter Gerber VEGF-modulated genes and methods employing them
DK1265914T3 (da) 2000-03-22 2008-03-10 Curagen Corp WNT-1-relaterede polypeptider og nukleinsyrer, der koder for samme
US6596488B2 (en) 2000-03-30 2003-07-22 City Of Hope Tumor suppressor gene
US6387624B1 (en) 2000-04-14 2002-05-14 Incyte Pharmaceuticals, Inc. Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US20020132350A1 (en) 2000-09-14 2002-09-19 Pioneer Hi-Bred International, Inc. Targeted genetic manipulation using Mu bacteriophage cleaved donor complex
US6709861B2 (en) 2000-11-17 2004-03-23 Lucigen Corp. Cloning vectors and vector components
AU2002239284A1 (en) 2000-11-27 2002-06-03 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US20030087232A1 (en) 2001-01-25 2003-05-08 Fred Christians Methods for screening polypeptides
US7807408B2 (en) 2001-03-19 2010-10-05 President & Fellows Of Harvard College Directed evolution of proteins
US6863833B1 (en) 2001-06-29 2005-03-08 The Board Of Trustees Of The Leland Stanford Junior University Microfabricated apertures for supporting bilayer lipid membranes
AU2002320294B2 (en) 2001-07-03 2009-01-15 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Mammalian sweet and amino acid heterodimeric taste receptors
US6852492B2 (en) 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
EP1487978B1 (en) 2002-03-15 2008-11-19 Nuevolution A/S An improved method for synthesising templated molecules
WO2003095669A1 (en) 2002-05-10 2003-11-20 The Texas A & M University System Stochastic sensing through covalent interactions
US7452699B2 (en) 2003-01-15 2008-11-18 Dana-Farber Cancer Institute, Inc. Amplification of DNA in a hairpin structure, and applications
WO2004072294A2 (en) 2003-02-12 2004-08-26 Genizon Svenska Ab Methods and means for nucleic acid sequencing
US20100035254A1 (en) 2003-04-08 2010-02-11 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
US7163658B2 (en) 2003-04-23 2007-01-16 Rouvain Bension Rapid sequencing of polymers
US7344882B2 (en) 2003-05-12 2008-03-18 Bristol-Myers Squibb Company Polynucleotides encoding variants of the TRP channel family member, LTRPC3
US20070122885A1 (en) 2005-08-22 2007-05-31 Fermalogic, Inc. Methods of increasing production of secondary metabolites by manipulating metabolic pathways that include methylmalonyl-coa
WO2005056750A2 (en) 2003-12-11 2005-06-23 Quark Biotech, Inc. Inversion-duplication of nucleic acids and libraries prepared thereby
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
US7238485B2 (en) 2004-03-23 2007-07-03 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
GB2413796B (en) 2004-03-25 2006-03-29 Global Genomics Ab Methods and means for nucleic acid sequencing
US20050227239A1 (en) * 2004-04-08 2005-10-13 Joyce Timothy H Microarray based affinity purification and analysis device coupled with solid state nanopore electrodes
US7618778B2 (en) * 2004-06-02 2009-11-17 Kaufman Joseph C Producing, cataloging and classifying sequence tags
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
US7700281B2 (en) 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
CA2577079C (en) 2004-08-13 2014-05-20 President And Fellows Of Harvard College An ultra high-throughput opti-nanopore dna readout platform
US20060086626A1 (en) * 2004-10-22 2006-04-27 Joyce Timothy H Nanostructure resonant tunneling with a gate voltage source
US7867716B2 (en) 2004-12-21 2011-01-11 The Texas A&M University System High temperature ion channels and pores
US7890268B2 (en) 2004-12-28 2011-02-15 Roche Molecular Systems, Inc. De-novo sequencing of nucleic acids
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US7507575B2 (en) 2005-04-01 2009-03-24 3M Innovative Properties Company Multiplex fluorescence detection device having removable optical modules
US7601499B2 (en) * 2005-06-06 2009-10-13 454 Life Sciences Corporation Paired end sequencing
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
WO2007018601A1 (en) 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
CA2633476C (en) 2005-12-22 2015-04-21 Pacific Biosciences Of California, Inc. Active surface coupled polymerases
US7932029B1 (en) 2006-01-04 2011-04-26 Si Lok Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities
EP1994179A2 (en) * 2006-02-18 2008-11-26 Michael Strathmann Massively multiplexed sequencing
US8673567B2 (en) * 2006-03-08 2014-03-18 Atila Biosystems, Inc. Method and kit for nucleic acid sequence detection
DK3239304T3 (da) 2006-04-04 2020-10-26 Keygene Nv Højgennemløbsdetektering af molekylære markører baseret på AFLP og sekventering med højt gennemløb
US8889348B2 (en) 2006-06-07 2014-11-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by nanopore using modified nucleotides
JP4876766B2 (ja) 2006-08-10 2012-02-15 トヨタ自動車株式会社 燃料電池
EP2059606A4 (en) 2006-09-06 2010-04-07 Phasebio Pharmaceuticals Inc PEPTIDE FUSION THERAPEUTIC COMPOSITIONS
WO2008045575A2 (en) 2006-10-13 2008-04-17 J. Craig Venter Institute, Inc. Sequencing method
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
GB2445016B (en) 2006-12-19 2012-03-07 Microsaic Systems Plc Microengineered ionisation device
WO2008102120A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
AU2008236694B2 (en) * 2007-04-04 2014-01-23 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
US8698481B2 (en) 2007-09-12 2014-04-15 President And Fellows Of Harvard College High-resolution molecular sensor
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
KR101414713B1 (ko) 2007-10-11 2014-07-03 삼성전자주식회사 리가제 및 엔도뉴클레아제의 존재하에서 롤링서클 증폭에의하여 표적 핵산을 증폭하는 방법
CN100554946C (zh) * 2007-12-18 2009-10-28 中国农业大学 一种双链核酸精确定量的方法
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
WO2009084721A1 (en) 2007-12-31 2009-07-09 Fujirebio Inc. Clusters of microresonators for cavity mode optical sensing
US8852864B2 (en) * 2008-01-17 2014-10-07 Sequenom Inc. Methods and compositions for the analysis of nucleic acids
US8263367B2 (en) 2008-01-25 2012-09-11 Agency For Science, Technology And Research Nucleic acid interaction analysis
US8231969B2 (en) 2008-03-26 2012-07-31 University Of Utah Research Foundation Asymmetrically functionalized nanoparticles
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2009120374A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
MX2010010600A (es) 2008-03-28 2011-03-30 Pacific Biosciences California Inc Composiciones y metodos para secuenciacion de acidos nucleicos.
WO2009132315A1 (en) * 2008-04-24 2009-10-29 Life Technologies Corporation Method of sequencing and mapping target nucleic acids
WO2009132124A2 (en) 2008-04-24 2009-10-29 The Trustees Of Columbia University In The City Of New York Geometric patterns and lipid bilayers for dna molecule organization and uses thereof
WO2010004265A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Enzyme-pore constructs
JP2011527191A (ja) 2008-07-07 2011-10-27 オックスフォード ナノポア テクノロジーズ リミテッド 塩基検出細孔
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
EP2334802A4 (en) * 2008-09-09 2012-01-25 Life Technologies Corp METHODS OF GENERATING SPECIFIC LIBRARIES OF GENES
US8481264B2 (en) 2008-09-19 2013-07-09 Pacific Biosciences Of California, Inc. Immobilized nucleic acid complexes for sequence analysis
CA3092369C (en) 2008-09-22 2025-09-23 University Of Washington MSP NANOPORES AND ASSOCIATED PROCESSES
WO2010036287A1 (en) 2008-09-24 2010-04-01 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US8383369B2 (en) 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
JP5794572B2 (ja) 2008-10-24 2015-10-14 エピセンター テクノロジーズ コーポレイションＥｐｉｃｅｎｔｒｅ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｃｏｒｐｏｒａｔｉｏｎ 核酸を修飾するためのトランスポゾン末端組成物及び方法
US8486630B2 (en) * 2008-11-07 2013-07-16 Industrial Technology Research Institute Methods for accurate sequence data and modified base position determination
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
CN102317473A (zh) * 2008-12-11 2012-01-11 加利福尼亚太平洋生物科学股份有限公司 核酸模板的分类
JP5843614B2 (ja) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
US20120100530A1 (en) 2009-01-30 2012-04-26 Oxford Nanopore Technologies Limited Enzyme mutant
CA2751470C (en) 2009-02-16 2016-07-26 Epicentre Technologies Corporation Template-independent ligation of single-stranded dna
JP5861223B2 (ja) 2009-02-23 2016-02-16 サイトムエックス セラピューティクス, インク.CytomX Therapeutics, Inc. プロタンパク質およびその使用方法
FR2943656A1 (fr) 2009-03-25 2010-10-01 Air Liquide Procede et installation de production d'hydrogene mettant en oeuvre un compresseur thermocinetique
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
US8986928B2 (en) 2009-04-10 2015-03-24 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
BRPI1012752B1 (pt) 2009-04-20 2019-06-25 Oxford Nanopore Technologies Limited Método e aparelho para detectar uma interação de uma entidade molecular com uma proteína de membrana em uma camada de moléculas anfifílicas
GB0910302D0 (en) 2009-06-15 2009-07-29 Lumora Ltd Nucleic acid amplification
US20120015821A1 (en) 2009-09-09 2012-01-19 Life Technologies Corporation Methods of Generating Gene Specific Libraries
CN102741430B (zh) 2009-12-01 2016-07-13 牛津楠路珀尔科技有限公司 生化分析仪器、用于进行生化分析的第一模块以及相关方法
US20120010085A1 (en) * 2010-01-19 2012-01-12 Rava Richard P Methods for determining fraction of fetal nucleic acids in maternal samples
FR2955773B1 (fr) 2010-02-01 2017-05-26 Commissariat Energie Atomique Complexe moleculaire de ciblage des antigenes vers les cellules presentatrices d'antigene et ses applications pour la vaccination
KR20110100963A (ko) * 2010-03-05 2011-09-15 삼성전자주식회사 미세 유동 장치 및 이를 이용한 표적 핵산의 염기 서열 결정 방법
US20110224106A1 (en) 2010-03-10 2011-09-15 Ibis Biosciences, Inc. Production Of Single-Stranded Circular Nucleic Acid
US8652779B2 (en) 2010-04-09 2014-02-18 Pacific Biosciences Of California, Inc. Nanopore sequencing using charge blockade labels
US20120244525A1 (en) 2010-07-19 2012-09-27 New England Biolabs, Inc. Oligonucleotide Adapters: Compositions and Methods of Use
EP2614156B1 (en) 2010-09-07 2018-08-01 The Regents of The University of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
CA2821299C (en) 2010-11-05 2019-02-12 Frank J. Steemers Linking sequence reads using paired code tags
EP2652153B1 (en) 2010-12-17 2017-07-05 The Trustees of Columbia University in the City of New York Dna sequencing by synthesis using modified nucleotides and nanopore detection
WO2012098561A2 (en) 2011-01-18 2012-07-26 Swamy R K A multipupurpose instrument for triangle solutions, measurements and geometrical applications called triometer
WO2012098562A2 (en) 2011-01-19 2012-07-26 Panacea Biotec Limited Liquid oral compositions of lanthanum salts
EP3037536B1 (en) 2011-01-28 2019-11-27 Illumina, Inc. Oligonucleotide replacement for di-tagged and directional libraries
US20120196279A1 (en) 2011-02-02 2012-08-02 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
AU2012215135B9 (en) 2011-02-11 2017-03-09 Oxford Nanopore Technologies Limited Mutant pores
US9347929B2 (en) 2011-03-01 2016-05-24 The Regents Of The University Of Michigan Controlling translocation through nanopores with fluid wall
SG10201604316WA (en) 2011-05-27 2016-07-28 Oxford Nanopore Tech Ltd Coupling method
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
US9145623B2 (en) 2011-07-20 2015-09-29 Thermo Fisher Scientific Oy Transposon nucleic acids comprising a calibration sequence for DNA sequencing
AU2012288629B2 (en) 2011-07-25 2017-02-02 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
EP3663412B1 (en) 2011-09-23 2022-03-09 Oxford Nanopore Technologies PLC Analysis of a polymer comprising polymer units by means of translocation through a nanopore
US20140308661A1 (en) 2011-09-25 2014-10-16 Theranos, Inc. Systems and methods for multi-analysis
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
US9404147B2 (en) 2011-12-19 2016-08-02 Gen-Probe Incorporated Closed nucleic acid structures
US9617591B2 (en) 2011-12-29 2017-04-11 Oxford Nanopore Technologies Ltd. Method for characterising a polynucleotide by using a XPD helicase
BR112014016112B8 (pt) 2011-12-29 2022-12-27 Oxford Nanopore Tech Ltd Método e aparelho para caracterizar um polinucleotídeo alvo, kit, e, uso de uma helicase
NO2694769T3 (https=) 2012-03-06 2018-03-03
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
US9116118B2 (en) 2012-06-08 2015-08-25 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
TWI655213B (zh) 2012-07-13 2019-04-01 Menicon Co., Ltd. 自我組織化肽衍生物的製造方法
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
EP2875128B8 (en) 2012-07-19 2020-06-24 Oxford Nanopore Technologies Limited Modified helicases
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
EP2895618B1 (en) 2012-09-14 2017-07-26 Oxford Nanopore Technologies Limited Sample preparation method
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
EP2911771B1 (en) 2012-10-26 2017-01-18 Oxford Nanopore Technologies Limited Droplet interfaces
EP2917732B1 (en) 2012-11-09 2016-09-07 Stratos Genomics Inc. Concentrating a target molecule for sensing by a nanopore
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US20140206842A1 (en) 2013-01-22 2014-07-24 Muhammed Majeed Peptides Modified with Triterpenoids and Small Organic Molecules: Synthesis and use in Cosmeceutical
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
KR102168813B1 (ko) 2013-03-08 2020-10-22 옥스포드 나노포어 테크놀로지즈 리미티드 효소 정지 방법
WO2014142850A1 (en) 2013-03-13 2014-09-18 Illumina, Inc. Methods and compositions for nucleic acid sequencing
JP6441893B2 (ja) 2013-03-19 2018-12-19 ディレクティド・ジェノミクス・エル・エル・シー 標的配列の濃縮
US10017814B2 (en) 2013-08-30 2018-07-10 University Of Washington Through Its Center For Commercialization Selective modification of polymer subunits to improve nanopore-based analysis
CN118256603A (zh) 2013-10-18 2024-06-28 牛津纳米孔科技公开有限公司 经修饰的酶
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201321123D0 (en) 2013-11-29 2014-01-15 Linea Ab Q Amplification of circular molecules
JP6749243B2 (ja) 2014-01-22 2020-09-02 オックスフォード ナノポール テクノロジーズ リミテッド １つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法
US20170226503A1 (en) 2014-02-06 2017-08-10 MetaMixis Biologics, Inc. Methods for Sequential Screening with Co-Culture Based Detection of Metagenomic Elements Conferring Heterologous Metabolite Secretion
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
WO2015148567A1 (en) 2014-03-24 2015-10-01 Bernick David L Molecular adapter for capture and manipulation of transfer rna
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
SG11201610357UA (en) 2014-06-13 2017-01-27 Illumina Cambridge Ltd Methods and compositions for preparing sequencing libraries
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
WO2016003814A1 (en) 2014-06-30 2016-01-07 Illumina, Inc. Methods and compositions using one-sided transposition
WO2016022557A1 (en) 2014-08-05 2016-02-11 Twist Bioscience Corporation Cell free cloning of nucleic acids
US10435685B2 (en) 2014-08-19 2019-10-08 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
EP3633047B1 (en) 2014-08-19 2022-12-28 Pacific Biosciences of California, Inc. Method of sequencing nucleic acids based on an enrichment of nucleic acids
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
WO2016138080A1 (en) 2015-02-24 2016-09-01 Trustees Of Boston University Protection of barcodes during dna amplification using molecular hairpins
CN107208162B (zh) 2015-02-26 2020-12-29 株式会社日立高新技术 核酸分子的构建方法
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN107488656B (zh) 2016-06-13 2020-07-17 陆欣华 一种核酸等温自扩增方法
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method

Also Published As

Publication number Publication date
US20200024655A1 (en) 2020-01-23
US10851409B2 (en) 2020-12-01
CA2843136A1 (en) 2013-01-31
EP2737084B1 (en) 2017-10-18
US11168363B2 (en) 2021-11-09
US20150152492A1 (en) 2015-06-04
JP2014531196A (ja) 2014-11-27
US11261487B2 (en) 2022-03-01
US20160281159A1 (en) 2016-09-29
CA2843136C (en) 2020-12-29
AU2012288629A1 (en) 2014-03-06
US20200239950A1 (en) 2020-07-30
US9957560B2 (en) 2018-05-01
KR20140050067A (ko) 2014-04-28
US12168799B2 (en) 2024-12-17
CN103827320B (zh) 2017-04-26
BR112014001699A2 (pt) 2017-06-13
US20220127669A1 (en) 2022-04-28
US20160362739A1 (en) 2016-12-15
WO2013014451A1 (en) 2013-01-31
IN2014DN00221A (https=) 2015-06-05
CN103827320A (zh) 2014-05-28
EP2737084A1 (en) 2014-06-04
AU2012288629B2 (en) 2017-02-02
US20180291441A1 (en) 2018-10-11
US10597713B2 (en) 2020-03-24

Similar Documents

Publication Publication Date Title
JP6298404B2 (ja) 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
JP6408494B2 (ja) 酵素停止法
JP6226869B2 (ja) 酵素法
KR101963918B1 (ko) 커플링 방법
US20190345550A1 (en) Method for controlling the movement of a polynucleotide through a transmembrane pore
CN105705656B (zh) 方法
EP3126516B1 (en) Method of target molecule characterisation using a molecular pore
CN109196116B (zh) 一种表征靶多核苷酸的方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150724

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20150724

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20160614

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20160909

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20161111

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20161214

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170328

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170623

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170825

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170928

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20180130

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20180223

R150 Certificate of patent or registration of utility model

Ref document number: 6298404

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250