JP6169847B2 - バイオリアクター内の培養パラメーターの制御方法 - Google Patents

バイオリアクター内の培養パラメーターの制御方法 Download PDF

Info

Publication number
JP6169847B2
JP6169847B2 JP2012545901A JP2012545901A JP6169847B2 JP 6169847 B2 JP6169847 B2 JP 6169847B2 JP 2012545901 A JP2012545901 A JP 2012545901A JP 2012545901 A JP2012545901 A JP 2012545901A JP 6169847 B2 JP6169847 B2 JP 6169847B2
Authority
JP
Japan
Prior art keywords
bioreactor
information
bag
culture
bags
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2012545901A
Other languages
English (en)
Japanese (ja)
Other versions
JP2013514804A (ja
Inventor
ゲバウアー，クラウス
リンドスコッグ，イヴァ
マグヌソン，ラース
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
Amersham Bioscience AB
GE Healthcare Bio Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44196030&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JP6169847(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Amersham Bioscience AB, GE Healthcare Bio Sciences Corp filed Critical Amersham Bioscience AB
Publication of JP2013514804A publication Critical patent/JP2013514804A/ja
Application granted granted Critical
Publication of JP6169847B2 publication Critical patent/JP6169847B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16—Vibrating; Shaking; Tilting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Computer Hardware Design (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2012545901A 2009-12-22 2010-12-20 バイオリアクター内の培養パラメーターの制御方法 Active JP6169847B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0951006 2009-12-22
SE0951006-6 2009-12-22
PCT/SE2010/051425 WO2011078773A1 (en) 2009-12-22 2010-12-20 A method for controlling culture parameters in a bioreactor

Publications (2)

Publication Number Publication Date
JP2013514804A JP2013514804A (ja) 2013-05-02
JP6169847B2 true JP6169847B2 (ja) 2017-07-26

Family

ID=44196030

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012545901A Active JP6169847B2 (ja) 2009-12-22 2010-12-20 バイオリアクター内の培養パラメーターの制御方法

Country Status (7)

Country Link
US (1) US20120258441A1 (enExample)
EP (1) EP2516682B1 (enExample)
JP (1) JP6169847B2 (enExample)
CN (1) CN102656282B (enExample)
CA (1) CA2784519A1 (enExample)
IN (1) IN2012DN05207A (enExample)
WO (1) WO2011078773A1 (enExample)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10260388B2 (en) 2006-11-16 2019-04-16 General Electric Company Sensing system and method
US9589686B2 (en) 2006-11-16 2017-03-07 General Electric Company Apparatus for detecting contaminants in a liquid and a system for use thereof
US9538657B2 (en) 2012-06-29 2017-01-03 General Electric Company Resonant sensor and an associated sensing method
US9536122B2 (en) 2014-11-04 2017-01-03 General Electric Company Disposable multivariable sensing devices having radio frequency based sensors
US9658178B2 (en) 2012-09-28 2017-05-23 General Electric Company Sensor systems for measuring an interface level in a multi-phase fluid composition
US10018613B2 (en) 2006-11-16 2018-07-10 General Electric Company Sensing system and method for analyzing a fluid at an industrial site
US10914698B2 (en) 2006-11-16 2021-02-09 General Electric Company Sensing method and system
US8455242B2 (en) 2010-02-22 2013-06-04 Hyclone Laboratories, Inc. Mixing system with condenser
US8542023B2 (en) 2010-11-09 2013-09-24 General Electric Company Highly selective chemical and biological sensors
US8365462B2 (en) 2011-05-31 2013-02-05 Heliae Development, Llc V-Trough photobioreactor systems
USD679965S1 (en) 2011-06-10 2013-04-16 Heliae Development, Llc Aquaculture vessel
USD682637S1 (en) 2011-06-10 2013-05-21 Heliae Development, Llc Aquaculture vessel
USD661164S1 (en) 2011-06-10 2012-06-05 Heliae Development, Llc Aquaculture vessel
EA030927B1 (ru) 2012-08-22 2018-10-31 Дженерал Электрик Компани Система и способ беспроводного измерения параметров рабочего состояния машины
US10598650B2 (en) 2012-08-22 2020-03-24 General Electric Company System and method for measuring an operative condition of a machine
WO2014033889A1 (ja) * 2012-08-31 2014-03-06 株式会社日立製作所 自動培養装置及び自動培養方法
US10684268B2 (en) 2012-09-28 2020-06-16 Bl Technologies, Inc. Sensor systems for measuring an interface level in a multi-phase fluid composition
ES2812582T3 (es) * 2013-06-17 2021-03-17 Cytiva Sweden Ab Sistema de biorreactor con un sensor de temperatura
ES2796274T3 (es) 2013-06-17 2020-11-26 Cytiva Sweden Ab Sistema de biorreactor que comprende un medio sensor de temperatura
EP3466509B1 (en) 2014-03-21 2021-03-10 Life Technologies Corporation Condenser systems for fluid processing systems
CN106232219B (zh) 2014-03-21 2020-04-24 生命科技股份有限公司 用于流体处理系统的气体过滤器系统
US9617566B2 (en) * 2014-07-11 2017-04-11 Lanzatech New Zealand Limited Control of bioreactor processes
US9457306B2 (en) 2014-10-07 2016-10-04 Life Technologies Corporation Regulated vacuum off-gassing of gas filter for fluid processing system and related methods
PL3209764T3 (pl) * 2014-10-23 2020-11-30 Cytiva Sweden Ab Układ bioreaktora
JP6675765B2 (ja) * 2015-02-05 2020-04-01 ゼネラル・エレクトリック・カンパニイ 細胞培養のためのバイオリアクタシステム
US10995312B2 (en) 2015-08-25 2021-05-04 Global Life Sciences Solutions Usa Llc Biomanufacturing apparatus
WO2017032847A1 (en) * 2015-08-25 2017-03-02 General Electric Company Improvements in and relating to biomanufacturing apparatus
EP3341467A1 (en) 2015-08-25 2018-07-04 General Electric Company Improvements in and relating to biomanufacturing apparatus
WO2017032830A1 (en) * 2015-08-25 2017-03-02 General Electric Company Improvements in and relating to biomanufacturing apparatus
US9920292B2 (en) * 2015-08-31 2018-03-20 General Electric Company System and method for initiating a cell culture
CN112403668B (zh) 2015-12-29 2023-06-27 生命科技股份有限公司 具有部分分割分隔区的柔性生物处理容器
TWI808057B (zh) * 2016-06-24 2023-07-11 瑞士商隆沙有限公司 可變直徑生物感測器容器及系統，在接種階段列及生產感測器中使用減少量之感測器生產醱酵產物之方法，及生物生產設施
JP6207708B2 (ja) * 2016-10-18 2017-10-04 株式会社日立製作所 自動培養装置、及び細胞培養方法
EP3532598B1 (en) * 2016-10-28 2020-09-09 Global Life Sciences Solutions USA LLC Bioreactor tray
EP3532602A1 (en) * 2016-10-31 2019-09-04 General Electric Company Bioreactor assembly
US12203061B2 (en) 2017-04-28 2025-01-21 Cytiva Sweden Ab System and method for enabling a single use wireless sensor by optimizing electrical power
WO2018197273A1 (en) * 2017-04-28 2018-11-01 Ge Healthcare Bio-Sciences Ab Method and system for creating reconfigurable bio-processing workflows
CN110537310B (zh) 2017-04-28 2023-11-10 思拓凡瑞典有限公司 用于为生物处理环境中的传感器无线提供功率的系统和方法
CN108795754A (zh) * 2018-04-23 2018-11-13 苏州欧飞纳米科技有限公司 生物反应器培养参数的控制系统及方法
GB201810772D0 (en) 2018-06-29 2018-08-15 Ge Healthcare Bio Sciences Ab Method in bioprocess purification system
EP3985097A1 (en) * 2020-10-14 2022-04-20 Sartorius Stedim Biotech GmbH A method and system for configuring and/or setup of a downstream process for processing a biomass
GB2616451B (en) * 2022-03-09 2024-11-27 Oribiotech Ltd A cell processing system for housing a bioreactor

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6029101A (en) * 1996-11-18 2000-02-22 Scius Corporation Process control system user interface
US6673008B1 (en) * 1998-04-28 2004-01-06 Ronald J. Thompson Fallopian tube and method of in vitro fertilization and embryo development
JP2000121511A (ja) * 1998-10-13 2000-04-28 Hitachi Koki Co Ltd 自動分離抽出装置及びその制御方法
EP1295393A2 (en) * 2000-06-13 2003-03-26 Azoteq (PTY) Limited Intelligent switch for connecting power to a load
US6934861B2 (en) * 2001-11-06 2005-08-23 Crosscheck Identification Systems International, Inc. National identification card system and biometric identity verification method for negotiating transactions
US6997043B2 (en) * 2002-08-15 2006-02-14 The Boeing Company Integration of atmospheric intrusion sensors in electronic component packages
US7604987B2 (en) * 2003-02-06 2009-10-20 National University Of Singapore Bioreactor for growing cell or tissue cultures
US20050015884A1 (en) * 2003-07-23 2005-01-27 Conaway Kenneth L. Self-interlocking resinous frame for sleeping mattresses
US20050051723A1 (en) * 2003-07-23 2005-03-10 Neagle Bradley D. Examination systems for biological samples
US7435581B2 (en) 2003-11-26 2008-10-14 Broadley-James Corporation Integrated bio-reactor monitor and control system
NZ551640A (en) * 2004-05-20 2010-05-28 Id Biomedical Corp Process for the production of an influenza vaccine
DE602005007423D1 (de) * 2004-06-02 2008-07-24 Millipore Corp Einwegreaktor
US7629167B2 (en) * 2004-06-04 2009-12-08 Xcellerex, Inc. Disposable bioreactor systems and methods
US7771988B2 (en) * 2005-03-24 2010-08-10 Hitachi, Ltd. Control device for fermenter
US20060275858A1 (en) * 2005-06-02 2006-12-07 Saucedo Victor M Optimization of Process Variables in Oxygen Enriched Fermentors Through Process Controls
JP5243038B2 (ja) 2005-11-01 2013-07-24 株式会社メディネット 細胞培養装置
EP1961806B1 (en) * 2006-05-13 2019-03-27 Pall Life Sciences Belgium Disposable bioreactor
WO2007139747A1 (en) * 2006-05-22 2007-12-06 Biovest International Inc. Interface of a cultureware module in a cell culture system and installation method thereof
EP2132534A1 (en) 2007-02-28 2009-12-16 Xcellerex, Inc. Weight measurements of liquids in flexible containers
CA2690384A1 (en) * 2007-06-22 2008-12-31 Algaedyne Corporation Bioreactor
US20100203624A1 (en) * 2007-09-26 2010-08-12 Ge Healthcare Bioscience Bioprocess Corp. Three dimensional disposable bioreactor

Also Published As

Publication number Publication date
EP2516682A1 (en) 2012-10-31
CA2784519A1 (en) 2011-06-30
EP2516682A4 (en) 2016-07-13
WO2011078773A1 (en) 2011-06-30
US20120258441A1 (en) 2012-10-11
EP2516682B1 (en) 2020-02-26
CN102656282A (zh) 2012-09-05
IN2012DN05207A (enExample) 2015-10-23
JP2013514804A (ja) 2013-05-02
CN102656282B (zh) 2015-09-02

Similar Documents

Publication Publication Date Title
JP6169847B2 (ja) バイオリアクター内の培養パラメーターの制御方法
Klöckner et al. Correlation between mass transfer coefficient kLa and relevant operating parameters in cylindrical disposable shaken bioreactors on a bench-to-pilot scale
Kadic et al. An introduction to bioreactor hydrodynamics and gas-liquid mass transfer
Stettler et al. Novel orbital shake bioreactors for transient production of CHO derived IgGs
Betts et al. Characterization and application of a miniature 10 mL stirred‐tank bioreactor, showing scale‐down equivalence with a conventional 7 L reactor
US20230123126A1 (en) Control Systems For A Fluid Mixing Apparatus
Von Stockar et al. Large-scale calorimetry and biotechnology
CN108018204A (zh) 一种平行生物反应器系统
Schaepe et al. kLa of stirred tank bioreactors revisited
Bolic et al. A flexible well-mixed milliliter-scale reactor with high oxygen transfer rate for microbial cultivations
US11773358B2 (en) Method for controlling culture parameters in a bioreactor
Dewasme et al. Linear robust control of S. cerevisiae fed-batch cultures at different scales
JP2014113109A (ja) 培地交換システムおよび培地加温装置
Tissot et al. Efficient and reproducible mammalian cell bioprocesses without probes and controllers?
US10989362B2 (en) Fluid processing control system and related methods
Zhu et al. Analysis of volumetric mass transfer coefficient (k L a) in small-(250 mL) to large-scale (2500 L) orbitally shaken bioreactors
Hill et al. Computational fluid dynamics based digital twins of fixed bed bioreactors validate scaling principles for recombinant adeno‐associated virus gene therapy manufacturing
Yu et al. A 3D analysis of oxygen transfer in a low-cost micro-bioreactor for animal cell suspension culture
Maischberger Optimized process and bioreactor characterization
JP5151932B2 (ja) 計測装置及び方法、並びに培養槽システムの運転装置及び方法
Warr Microbioreactors and scale-down models: Growth of CHO cells using the Pall Micro24 MicroReactor system
JP2025136306A (ja) 培養制御システム、培養制御方法、培養システム、及び培養方法
Isailovic et al. Fluid dynamics of a single-use, stirred-tank bioreactor for mammalian cell culture
Ruiz et al. A set membership approach to oxygen transport modeling with unmodeled dynamics
León‐Rodríguez et al. Design and characterization of a one‐compartment scale‐down system for simulating dissolved oxygen tension gradients

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20131206

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20141126

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20141209

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150309

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20150309

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20150804

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20151204

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20160128

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20160318

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20161228

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170203

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170303

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170403

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20170629

R150 Certificate of patent or registration of utility model

Ref document number: 6169847

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R157 Certificate of patent or utility model (correction)

Free format text: JAPANESE INTERMEDIATE CODE: R157

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

S533 Written request for registration of change of name

Free format text: JAPANESE INTERMEDIATE CODE: R313533

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250