JP6093505B2 - スーパーコンティニュームレーザパルスの様々なスペクトル色のパルス合成器 - Google Patents
スーパーコンティニュームレーザパルスの様々なスペクトル色のパルス合成器 Download PDFInfo
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- JP6093505B2 JP6093505B2 JP2012028232A JP2012028232A JP6093505B2 JP 6093505 B2 JP6093505 B2 JP 6093505B2 JP 2012028232 A JP2012028232 A JP 2012028232A JP 2012028232 A JP2012028232 A JP 2012028232A JP 6093505 B2 JP6093505 B2 JP 6093505B2
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
- G01J3/0218—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using optical fibers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
- G01J3/4406—Fluorescence spectrometry
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B2207/00—Coding scheme for general features or characteristics of optical elements and systems of subclass G02B, but not including elements and systems which would be classified in G02B6/00 and subgroups
- G02B2207/114—Two photon or multiphoton effect
Description
22 レーザ装置
24 レーザユニット
26 単色レーザ光パルス
28 スペクトル分散素子
30 時間がずらされた光成分を有する白色光レーザパルス
32 光学素子
34 スペクトル分散レーザ光パルス
36 補正ユニット
38 白色光レーザパルス
40 顕微鏡
42 第1のレンズ
44 照明ピンホール
46 ビームスプリッタ
48 第2のレンズ
49 ビーム軸
50 走査装置
52 第3のレンズ
54 第4のレンズ
56 対物系
58 第5のレンズ
60 第6のレンズ
62 試料スライド
64 検出ピンホール
66 検出装置
70 波長−時間図
71 第1の光成分
72 第2の光成分
73 第3の光成分
74 第4の光成分
75 第5の光成分
76 第6の光成分
80 補正素子
82 入力セクション
84 テーパセクション
86 出力セクション
88 第1の断熱遷移
90 第2の断熱遷移
92 ファイバ芯
94 ファイバの厚いセクション内の光パルス
96 ファイバの超薄セクション内の光パルス
98 ファイバの超薄セクション内の分散補正光パルス
100 ファイバの厚いセクション内の分散補正光パルス
110 波長−分散図
S2〜S10 ステップ2〜ステップ10
Claims (11)
- 少なくとも1つの広帯域レーザ光パルス(30)を生成するレーザユニット(24)と、前記広帯域レーザ光パルス(30)からレーザ光の狭いスペクトル波長帯を分離する音響光学調整可能フィルタ(32;AOTF)と、前記広帯域レーザ光パルス(30)の経路に配置された補正ユニット(36)とを備えて構成される、顕微鏡(40)のための照明装置(20)であって、前記広帯域レーザ光パルス(30)の異なる波長の光成分(71、72、73、74、75、76)が前記音響光学調整可能フィルタ(32;AOTF)を通過した後に互いに時間的にずらされ、スペクトル的に制限され、互いにスペクトル的に離間され、前記音響光学調整可能フィルタ(32;AOTF)の下流側の前記補正ユニット(36)は、前記光成分(71、72、73、74、75、76)を時間的にずらして、前記光成分(71、72、73、74、75、76)が前記補正ユニット(36)を同時に出る、照明装置(20)。
- 前記補正ユニット(36)は、微小構造光誘導ファイバの形態をした補正素子(80)を含む、請求項1に記載の照明装置(20)。
- 前記補正ユニット(36)は、超薄ガラス繊維又はテーパ形繊維の形態をした補正素子(80)を含む、請求項1または2に記載の照明装置(20)。
- 前記微小構造光誘導ファイバは、ガラス芯又は空気芯を有する、請求項2に記載の照明装置(20)。
- 前記微小構造光誘導ファイバの分散は、前記ファイバの構造および/または長さによって決まる、請求項2または4に記載の照明装置(20)。
- 前記微小構造光誘導ファイバの構造は、断熱的に変わる、請求項5に記載の照明装置(20)。
- 前記微小構造光誘導ファイバは、回転対称な設計ではない、請求項2、4〜6のいずれか一項に記載の照明装置(20)。
- 前記広帯域レーザ光パルスは、前記照明装置を励起させた後、高度に時間分解された顕微鏡測定、例えば、蛍光寿命イメージング(FLIM)に使用される、請求項1〜7のいずれか一項に記載の照明装置(20)。
- 物体を検査する顕微鏡(40)であって、請求項1〜8のいずれか一項に記載の照明装置(20)に結合される、顕微鏡(40)。
- 共焦点走査型顕微鏡である、請求項9に記載の顕微鏡(40)。
- 顕微鏡(40)のための照明光線を生成する方法であって、互いに時間的にずらされた異なる波長の光成分(71、72、73、74、75、76)を有する広帯域レーザ光パルス(30)が生成される、方法において、
音響光学調整可能フィルタ(32;AOTF)によって、前記広帯域レーザ光パルス(30)からレーザ光の狭いスペクトル波長帯を分離して、前記音響光学調整可能フィルタ(32;AOTF)を通過した後に前記光成分(71、72、73、74、75、76)が互いに時間的にずらされ、スペクトル的に制限され、互いにスペクトル的に離間される、ステップと、
時間的にずらされた光成分(71、72、73、74、75、76)を補正ユニット(36)に結合するステップと、
前記光成分(71、72、73、74、75、76)を、前記補正ユニット(36)内で時間的にずらして、前記光成分(71、72、73、74、75、76)が前記補正ユニット(36)を同時に出るステップと、
を有する、方法。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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DE102011000905A DE102011000905A1 (de) | 2011-02-24 | 2011-02-24 | Pulsvereiniger für die verschiedenen Spektralfarben eines Superkontinuum-Lasers |
DE102011000905.1 | 2011-02-24 |
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JP2012177919A JP2012177919A (ja) | 2012-09-13 |
JP6093505B2 true JP6093505B2 (ja) | 2017-03-08 |
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JP2012028232A Active JP6093505B2 (ja) | 2011-02-24 | 2012-02-13 | スーパーコンティニュームレーザパルスの様々なスペクトル色のパルス合成器 |
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US (1) | US8879148B2 (ja) |
EP (1) | EP2492737B1 (ja) |
JP (1) | JP6093505B2 (ja) |
CN (1) | CN102681157B (ja) |
DE (1) | DE102011000905A1 (ja) |
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CN113155795B (zh) * | 2021-04-15 | 2023-03-21 | 西北核技术研究所 | 掺稀土元素光纤激光上能级荧光寿命直接测量装置和方法 |
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US5862287A (en) * | 1996-12-13 | 1999-01-19 | Imra America, Inc. | Apparatus and method for delivery of dispersion compensated ultrashort optical pulses with high peak power |
US6020988A (en) * | 1997-08-05 | 2000-02-01 | Science Research Laboratory, Inc. | Ultra high resolution wave focusing method and apparatus and systems employing such method and apparatus |
JP4282839B2 (ja) * | 1999-08-20 | 2009-06-24 | 独立行政法人科学技術振興機構 | 超短パルス広帯域光波発生方法及びその装置 |
DE10115589B4 (de) | 2000-06-17 | 2020-07-30 | Leica Microsystems Cms Gmbh | Konfokales Scanmikroskop |
US6954575B2 (en) * | 2001-03-16 | 2005-10-11 | Imra America, Inc. | Single-polarization high power fiber lasers and amplifiers |
JP3757854B2 (ja) * | 2001-12-06 | 2006-03-22 | 株式会社島津製作所 | 複数の蛍光物質を含む試料の分析方法及び装置 |
DE10247247A1 (de) * | 2002-10-10 | 2004-04-22 | Leica Microsystems Heidelberg Gmbh | Optische Anordnung und Mikroskop |
DE10259443B4 (de) * | 2002-12-19 | 2015-01-22 | Carl Zeiss Microscopy Gmbh | Verfahren und Anordnung zur optischen Untersuchung und/oder Bearbeitung einer Probe |
US6914720B2 (en) * | 2003-03-27 | 2005-07-05 | Riken | Time resolved fluorescence microscope |
US7024078B2 (en) * | 2003-11-03 | 2006-04-04 | University Of Rochester | Fiber device with high nonlinearity, dispersion control and gain |
JP2005172776A (ja) * | 2003-12-07 | 2005-06-30 | Kazuyoshi Ito | 複数の波長依存性物質を含む試料の分析方法及び分析装置 |
JP4869734B2 (ja) * | 2005-04-25 | 2012-02-08 | オリンパス株式会社 | 多光子励起走査型レーザ顕微鏡 |
US8120778B2 (en) * | 2009-03-06 | 2012-02-21 | Imra America, Inc. | Optical scanning and imaging systems based on dual pulsed laser systems |
WO2007112449A2 (en) * | 2006-03-28 | 2007-10-04 | The Regents Of The University Of California | Apparatus and method for raman spectroscopy and microscopy with time domain spectral analysis |
JP5248804B2 (ja) | 2007-04-20 | 2013-07-31 | オリンパス株式会社 | 超短光パルスの光ファイバ伝送装置、およびこれを有する光学システム |
JP5307439B2 (ja) | 2007-04-23 | 2013-10-02 | オリンパス株式会社 | レーザ顕微鏡 |
US9285575B2 (en) * | 2009-01-26 | 2016-03-15 | President And Fellows Of Harvard College | Systems and methods for selective detection and imaging in coherent Raman microscopy by spectral excitation shaping |
DE102010037190B4 (de) * | 2010-08-27 | 2015-11-26 | Leica Microsystems Cms Gmbh | Vorrichtung zum zeitlichen Verschieben von Weißlichtlaserpulsen |
US8432543B2 (en) * | 2010-09-20 | 2013-04-30 | Robert D Frankel | Method and system for raman, fluorescence, lithographic, stimulated emission and photochemical imaging beyond the diffraction limit |
EP2458750A3 (en) | 2010-11-30 | 2017-01-25 | Olympus Corporation | Optical fiber delivery system for delivering optical short pulses and optical fiber delivery method |
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2011
- 2011-02-24 DE DE102011000905A patent/DE102011000905A1/de not_active Ceased
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2012
- 2012-02-07 CN CN201210026046.1A patent/CN102681157B/zh active Active
- 2012-02-13 JP JP2012028232A patent/JP6093505B2/ja active Active
- 2012-02-22 US US13/402,007 patent/US8879148B2/en not_active Expired - Fee Related
- 2012-02-23 EP EP12156692.1A patent/EP2492737B1/de active Active
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CN102681157B (zh) | 2016-09-21 |
US8879148B2 (en) | 2014-11-04 |
DE102011000905A1 (de) | 2012-08-30 |
EP2492737A3 (de) | 2012-12-05 |
US20120218631A1 (en) | 2012-08-30 |
JP2012177919A (ja) | 2012-09-13 |
EP2492737B1 (de) | 2020-06-03 |
CN102681157A (zh) | 2012-09-19 |
EP2492737A2 (de) | 2012-08-29 |
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