JP6085035B2 - コード化された高分子微粒子 - Google Patents
コード化された高分子微粒子 Download PDFInfo
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- JP6085035B2 JP6085035B2 JP2015542957A JP2015542957A JP6085035B2 JP 6085035 B2 JP6085035 B2 JP 6085035B2 JP 2015542957 A JP2015542957 A JP 2015542957A JP 2015542957 A JP2015542957 A JP 2015542957A JP 6085035 B2 JP6085035 B2 JP 6085035B2
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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Description
本発明の一実施例によれば、コード化された高分子微粒子コア;及び前記微粒子コアを取り囲むシリカシェルを含むコード化された高分子微粒子が提供される。
3−(トリメトキシシリル)プロピルアクリレート(TMSPA)であることができる。
N,N1−メチレンビスアクリルアミド、メチレンビスメタクリルアミド及びジメタクリル酸テトラエチレングリコールなどがあげられる。
次の段階S3で、前記コード化された高分子微粒子コアにシリカ前駆体を処理して前記コード化された高分子微粒子コア上にシリカシェルを形成することによって、コード化された高分子微粒子が製造される。前記シリカシェルの形成は多様な公知された方法によって遂行することができ、たとえば修正されたStober方法を使用することができる。
Claims (12)
- コード化された高分子微粒子コアと、
前記微粒子コアを取り囲んだシリカシェルを含み、
前記コード化された高分子微粒子コアと前記シリカシェルが−Si−O−Si−結合で連結された
ことを特徴とするコード化された高分子微粒子。 - 前記高分子は光硬化性高分子である
請求項1に記載のコード化された高分子微粒子。 - 前記微粒子コアに磁性ナノ粒子を含む
請求項1に記載のコード化された高分子微粒子。 - 前記微粒子コアと前記シリカシェルの間に磁性ナノ粒子が導入された
請求項1に記載のコード化された高分子微粒子。 - 前記シリカシェル表面にカルボキシル基またはアミノ基が導入された
請求項1に記載のコード化された高分子微粒子。 - 前記シリカシェル表面に抗原、抗体、DNA,RNA及びオリゴヌクレオチドからなる群中で選択されるいずれか一つのバイオ物質が導入された
請求項1ないし5のいずれかに記載のコード化された高分子微粒子。 - 光硬化性物質、及び前記光硬化性物質と重合可能な官能基とアルコキシシリル基を共に具備したリンカーの混合物を提供する段階と;パターン化されたエネルギーを印加して前記混合物を硬化し、コード化することによってコード化された高分子微粒子コアを得る段階と;及び前記コード化された高分子微粒子コアにシリカ前駆体を処理して前記コード化された高分子微粒子コア上にシリカシェルを形成する段階を含み、
前記コード化された微粒子コアの表面にグラフトされた前記アルコキシシリル基と前記シリカ前駆体の反応によって、前記コアと前記シリカシェル界面に−Si−O−Si−結合が形成される
ことを特徴とするコード化された高分子微粒子の製造方法。 - 前記高分子微粒子コアを得る段階は、光流体マスクレスリソグラフィによって遂行される
請求項7記載のコード化された高分子微粒子の製造方法。 - 前記混合物に磁性ナノ粒子を追加する段階を含む
請求項7に記載のコード化された高分子微粒子の製造方法。 - 前記コード化された高分子微粒子コアに親水性高分子がコーティングされた磁性ナノ粒子を付着させる段階を含む
請求項7に記載のコード化された高分子微粒子の製造方法。 - 前記シリカシェル表面を改質してカルボキシル基またはアミノ基を導入する
請求項7に記載のコード化された高分子微粒子の製造方法。 - バイオ物質を前記シリカシェル表面に結合させる段階を含む
請求項7ないし11のいずれかに記載のコード化された高分子微粒子の製造方法。
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Publication number | Priority date | Publication date | Assignee | Title |
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JP6182520B2 (ja) * | 2014-12-01 | 2017-08-16 | 三洋化成工業株式会社 | 磁性シリカ粒子、該磁性シリカ粒子を用いた測定対象物質測定方法及び測定対象物質測定用試薬 |
KR101758145B1 (ko) * | 2015-02-16 | 2017-07-17 | 한국과학기술연구원 | 바이오 어세이를 위한 마이크로 입자의 제조방법 및 이에 의해 제조된 바이오 어세이를 위한 마이크로 입자 |
US20160322560A1 (en) * | 2015-04-30 | 2016-11-03 | The Regents Of The University Of California | 3d piezoelectric polymer materials and devices |
KR102435668B1 (ko) * | 2015-10-20 | 2022-08-24 | 주식회사 퀀타매트릭스 | 다중 분석 칩 및 이를 이용한 분석 장치 |
US11987693B2 (en) | 2016-10-05 | 2024-05-21 | Seoul National University R&Db Foundation | Biochemical carriers capable of storage, preservation and indexing and method for fabricating the same |
KR101879585B1 (ko) * | 2016-10-06 | 2018-07-18 | 경희대학교 산학협력단 | 하이드로겔 입자의 자동 코팅 장치 및 방법 |
KR101850854B1 (ko) * | 2016-10-17 | 2018-04-20 | 연세대학교 원주산학협력단 | 퀀타매트릭스 어세이 플랫폼 기반 그람양성, 그람음성균 및 캔디다의 검출 및 동정과 항생제 내성여부를 동시 확인할 수 있는 진단법 및 그 키트 |
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WO2018102631A1 (en) * | 2016-12-01 | 2018-06-07 | University Of Florida Research Foundation, Inc. | Polymer conjugates, methods of making polymer conjugates, and methods of using polymer conjugates |
KR101905800B1 (ko) | 2017-02-13 | 2018-10-08 | 충남대학교산학협력단 | 고분자-나노실리카 복합체 및 그의 제조방법 |
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JP7091129B2 (ja) * | 2018-04-27 | 2022-06-27 | キヤノン株式会社 | 粒子、及びその製造方法 |
GB201818421D0 (en) * | 2018-11-12 | 2018-12-26 | Cambridge Entpr Ltd | Magnetic particle and method |
JP7363327B2 (ja) * | 2019-10-09 | 2023-10-18 | 東ソー株式会社 | 抗体結合用磁性粒子およびその製造方法 |
JP7499570B2 (ja) * | 2019-10-15 | 2024-06-14 | キヤノン株式会社 | 粒子、およびその製造方法、並びにその粒子を用いた標的物質の検出方法 |
Family Cites Families (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL111186A (en) * | 1994-10-06 | 1999-09-22 | Univ Bar Ilan | Process for the preparation of microspheres and microspheres made thereby |
JP2000169591A (ja) * | 1998-12-11 | 2000-06-20 | Ube Nitto Kasei Co Ltd | 光硬化性樹脂被覆粒子、その製造方法および液晶表示素子の製造方法 |
US6548264B1 (en) * | 2000-05-17 | 2003-04-15 | University Of Florida | Coated nanoparticles |
US6790672B2 (en) * | 2001-02-19 | 2004-09-14 | Board Of Regents The University Of Texas System | Encoded molecular sieve particle-based sensors |
GB0220063D0 (en) * | 2002-08-29 | 2002-10-09 | Isis Innovation | Magnetic particle and process for preparation |
US20040101822A1 (en) * | 2002-11-26 | 2004-05-27 | Ulrich Wiesner | Fluorescent silica-based nanoparticles |
CN1317560C (zh) * | 2005-10-19 | 2007-05-23 | 华中科技大学 | 一种提高量子点编码微球的编码稳定性的方法 |
US7709544B2 (en) | 2005-10-25 | 2010-05-04 | Massachusetts Institute Of Technology | Microstructure synthesis by flow lithography and polymerization |
JP4780710B2 (ja) * | 2006-03-16 | 2011-09-28 | 大阪府 | コア−シェル型高分子ゲル微粒子及びその製造方法 |
GB0617480D0 (en) * | 2006-09-06 | 2006-10-18 | Univ Sheffield | Novel nanoparticles |
GB2445579A (en) | 2007-01-11 | 2008-07-16 | Secr Defence | An encoded microsphere |
GB2445580A (en) | 2007-01-11 | 2008-07-16 | Secr Defence | An encoded microsphere |
KR20100029613A (ko) | 2008-09-08 | 2010-03-17 | 엘지전자 주식회사 | 차량 네비게이션 방법 및 그 장치 |
KR101004769B1 (ko) * | 2008-09-09 | 2011-01-04 | 서울대학교산학협력단 | 광유체적 리소그래피 시스템 및 마이크로구조물 제조방법 |
CN102272239A (zh) * | 2008-11-26 | 2011-12-07 | 爱尔兰国家大学科克学院 | 制备二氧化硅微粒的方法 |
KR101368606B1 (ko) * | 2009-04-14 | 2014-02-28 | 서울대학교산학협력단 | 컬러 코드화된 자성 구조물 |
ES2781572T3 (es) * | 2009-04-14 | 2020-09-03 | Univ California | Procedimiento de creación de materiales coloreados fijando estructuras ordenadas de nanopartículas de magnetita dentro de un medio sólido |
KR101125191B1 (ko) * | 2009-11-09 | 2012-03-19 | 한국과학기술원 | 콜로이드 입자로 안정화된 액적을 이용한 표면구조를 갖는 미세입자의 제조방법 |
US10013647B2 (en) * | 2010-03-31 | 2018-07-03 | Quantamatrix Inc. | Method for magnetically controlling a magnetic structure |
CN101912757B (zh) * | 2010-08-30 | 2012-06-27 | 武汉大学 | 一种荧光-磁性双编码微球的制备方法 |
CN102120168B (zh) * | 2010-12-07 | 2013-05-29 | 复旦大学 | 多功能核壳结构荧光编码磁性微球及其制备方法 |
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