JP6071886B2 - 脳損傷のバイオマーカー - Google Patents
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
- G01N33/6896—Neurological disorders, e.g. Alzheimer's disease
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- G01N2800/00—Detection or diagnosis of diseases
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- G01N2800/2871—Cerebrovascular disorders, e.g. stroke, cerebral infarct, cerebral haemorrhage, transient ischemic event
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Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2665245T3 (es) | 2008-08-11 | 2018-04-25 | Banyan Biomarkers, Inc. | Proceso de detección de biomarcador y ensayo de estado neurológico |
| EP2825893A4 (en) | 2012-03-13 | 2016-03-16 | Univ Johns Hopkins | CITRILLINATED BRAIN AND NERVE PROTEINS AS A BIOMARKER OF BRAIN INJURY OR NERVOUS GENERATION |
| US9378551B2 (en) * | 2013-01-03 | 2016-06-28 | Siemens Aktiengesellschaft | Method and system for lesion candidate detection |
| EP3022322A4 (en) | 2013-07-17 | 2017-05-17 | The Johns Hopkins University | A multi-protein biomarker assay for brain injury detection and outcome |
| JP6312302B2 (ja) * | 2014-01-06 | 2018-04-18 | 公益財団法人ヒューマンサイエンス振興財団 | 脳梗塞の診断マーカー |
| EP2896702A1 (en) * | 2014-01-16 | 2015-07-22 | University College Cork, National University Of Irland Cork | A method of identifying a neonate at risk of having or developing hypoxic-ischaemic encephalopathy (HIE) |
| EP3166613A4 (en) | 2014-07-09 | 2018-02-21 | Duke University | Compositions and methods for the repair of myelin |
| EP3702786A1 (en) * | 2014-10-06 | 2020-09-02 | Université de Genève | Markers and their use in brain injury |
| US12422442B2 (en) | 2014-10-06 | 2025-09-23 | Universite De Geneve | Markers and their use in brain injury |
| US20180024145A1 (en) | 2015-02-05 | 2018-01-25 | Immunarray USA, Inc. | Methods and compositions for diagnosing brain injury or neurodegeneration |
| JP6839854B2 (ja) | 2015-05-05 | 2021-03-17 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California | 星状細胞トラウマトーム(traumatome)および神経外傷のバイオマーカー |
| ES3001985T3 (en) * | 2016-10-03 | 2025-03-06 | Abbott Lab | Improved methods of assessing uch-l1 in patient samples |
| CN110366558A (zh) | 2016-10-28 | 2019-10-22 | 班扬生物标记公司 | 针对泛素c末端水解酶l1(uch-l1)和胶质纤维酸性蛋白(gfap)的抗体及相关方法 |
| US20200191801A1 (en) * | 2016-12-23 | 2020-06-18 | Susanne PANGRATZ-FÜHRER | Alpha-b crystallin in the diagnosis of neonatal brain damage |
| CN106645755B (zh) * | 2016-12-30 | 2018-09-25 | 深圳大学 | 一种ad生物标记物及其检测方法 |
| WO2018132676A1 (en) | 2017-01-13 | 2018-07-19 | Duke University | Compositions and methods for the treatment of myelin related and inflammation related diseases or disorders |
| US11016092B2 (en) | 2017-03-23 | 2021-05-25 | Abbott Laboratories | Methods for aiding in the diagnosis and determination of the extent of traumatic brain injury in a human subject using the early biomarker ubiquitin carboxy-terminal hydrolase L1 |
| BR112019021612A2 (pt) | 2017-04-15 | 2020-05-12 | Abbott Laboratories | Métodos para auxiliar o diagnóstico hiperagudo e determinação de traumatismo crânioencefálico em um indivíduo humano com o uso de biomarcadores precoces |
| EP3635407B1 (en) | 2017-04-28 | 2025-09-10 | Abbott Laboratories | Methods for aiding in the hyperacute diagnosis and determination of traumatic brain injury using early biomarkers on at least two samples from the same human subject |
| US11709168B2 (en) | 2017-05-23 | 2023-07-25 | Brainbox Solutions, Inc. | Biomarker levels and neuroimaging for detecting, monitoring and treating brain injury or trauma |
| US10866251B2 (en) | 2017-05-25 | 2020-12-15 | Abbott Laboratories | Methods for aiding in the determination of whether to perform imaging on a human subject who has sustained or may have sustained an injury to the head using early biomarkers |
| CN110709704B (zh) | 2017-05-30 | 2024-06-21 | 雅培实验室 | 利用心肌肌钙蛋白i辅助诊断和评估人类受试者的轻度创伤性脑损伤的方法 |
| CA3068041C (en) | 2017-07-03 | 2024-06-25 | Abbott Laboratories | Improved methods for measuring ubiquitin carboxy-terminal hydrolase l1 levels in blood |
| WO2019112863A1 (en) * | 2017-12-06 | 2019-06-13 | FloTBI Inc. | Assay and point of care device utilizing saliva for diagnosis and treatment of neurological conditions affected brain health |
| CA3067057A1 (en) | 2017-12-09 | 2019-06-13 | Abbott Laboratories | Methods for aiding in the diagnosis and evaluation of a subject who has sustained an orthopedic injury and that has or may have sustained an injury to the head, such as mild traumatic brain injury (tbi), using glial fibrillary acidic protein (gfap) and/or ubiquitin carboxy-terminal hydrolase l1 (uch-l1) |
| US11016105B2 (en) | 2017-12-09 | 2021-05-25 | Abbott Laboratories | Methods for aiding in diagnosing and evaluating a traumatic brain injury in a human subject using a combination of GFAP and UCH-L1 |
| EP3732489B1 (en) | 2017-12-29 | 2025-10-22 | Abbott Laboratories | Novel biomarkers and methods for diagnosing and evaluating traumatic brain injury |
| CN108872589B (zh) * | 2018-01-03 | 2021-09-03 | 深圳市人民医院 | 脑梗死外周血标志物及其应用 |
| CN111919121A (zh) * | 2018-03-30 | 2020-11-10 | 国立研究开发法人国立精神·神经医疗研究中心 | 新生儿缺氧缺血性脑病的严重程度判定方法及预后预测方法 |
| WO2020102524A1 (en) * | 2018-11-14 | 2020-05-22 | Mark Evans | Method and apparatus for reducing the risk of neonatal neurological injury |
| US20220016221A1 (en) * | 2018-12-05 | 2022-01-20 | Ipsen Biopharm Limited | Treatment of symptoms of traumatic brain injury |
| CN110261617B (zh) * | 2019-05-14 | 2022-07-01 | 深圳市人民医院 | 脑出血外周血标志物及其应用 |
| CN110907422B (zh) * | 2019-12-16 | 2021-07-06 | 吉林大学 | 基于Ti3C2的凝血酶适体传感器及其制备方法 |
| WO2021234607A1 (en) * | 2020-05-20 | 2021-11-25 | St. Jude Children's Research Hospital | Detection of alzheimer's disease using specific biomarkers |
| EP3982123A1 (en) * | 2020-10-08 | 2022-04-13 | Fundació Hospital Universitari Vall d'Hebron - Institut de Recerca | Markers and their use in brain injury |
| US20240108276A1 (en) * | 2021-02-01 | 2024-04-04 | The University Of Chicago | Systems and Methods for Identifying Progression of Hypoxic-Ischemic Brain Injury |
Family Cites Families (71)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3234412A (en) * | 1961-07-21 | 1966-02-08 | Babcock & Wilcox Co | Thermionic conversion nuclear reactor |
| SE462454B (sv) | 1988-11-10 | 1990-06-25 | Pharmacia Ab | Maetyta foer anvaendning i biosensorer |
| US5660985A (en) | 1990-06-11 | 1997-08-26 | Nexstar Pharmaceuticals, Inc. | High affinity nucleic acid ligands containing modified nucleotides |
| US5637459A (en) | 1990-06-11 | 1997-06-10 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: chimeric selex |
| US5567588A (en) | 1990-06-11 | 1996-10-22 | University Research Corporation | Systematic evolution of ligands by exponential enrichment: Solution SELEX |
| US5707796A (en) | 1990-06-11 | 1998-01-13 | Nexstar Pharmaceuticals, Inc. | Method for selecting nucleic acids on the basis of structure |
| US5683867A (en) | 1990-06-11 | 1997-11-04 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: blended SELEX |
| DE69128350T2 (de) | 1990-06-11 | 1998-03-26 | Nexstar Pharmaceuticals Inc | Nukleinsäureliganden |
| US5496938A (en) | 1990-06-11 | 1996-03-05 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands to HIV-RT and HIV-1 rev |
| US5270163A (en) | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
| US6011020A (en) | 1990-06-11 | 2000-01-04 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligand complexes |
| DE69432791T2 (de) | 1993-05-28 | 2004-06-03 | Baylor College Of Medicine, Houston | Verfahren und massenspektrometer zur desorption und ionisierung von analyten |
| US5866434A (en) | 1994-12-08 | 1999-02-02 | Meso Scale Technology | Graphitic nanotubes in luminescence assays |
| US6673533B1 (en) | 1995-03-10 | 2004-01-06 | Meso Scale Technologies, Llc. | Multi-array multi-specific electrochemiluminescence testing |
| US6140045A (en) | 1995-03-10 | 2000-10-31 | Meso Scale Technologies | Multi-array, multi-specific electrochemiluminescence testing |
| NZ306051A (en) | 1995-03-10 | 1999-11-29 | Meso Scale Technologies Llc | Testing using electrochemiluminescence |
| US6207369B1 (en) | 1995-03-10 | 2001-03-27 | Meso Scale Technologies, Llc | Multi-array, multi-specific electrochemiluminescence testing |
| US6319670B1 (en) | 1995-05-09 | 2001-11-20 | Meso Scale Technology Llp | Methods and apparatus for improved luminescence assays using microparticles |
| NZ516848A (en) | 1997-06-20 | 2004-03-26 | Ciphergen Biosystems Inc | Retentate chromatography apparatus with applications in biology and medicine |
| US6413783B1 (en) | 1997-09-18 | 2002-07-02 | Meso Scale Technologies, Llc | Assay sonication apparatus and methodology |
| JP2002510505A (ja) | 1998-04-03 | 2002-04-09 | フィロス インク. | 位置特定可能な蛋白質アレイ |
| US6406921B1 (en) | 1998-07-14 | 2002-06-18 | Zyomyx, Incorporated | Protein arrays for high-throughput screening |
| US6780606B1 (en) | 1999-02-26 | 2004-08-24 | Synx Pharma, Inc. | Method for diagnosing and distinguishing stroke and diagnostic devices for use therein |
| AU4025300A (en) | 1999-03-24 | 2000-10-09 | Packard Bioscience Company | Continuous porous matrix arrays |
| JP5064625B2 (ja) | 1999-10-27 | 2012-10-31 | バイオウルフ テクノロジーズ エルエルスィー | パターンを同定するための方法及び機械 |
| CN1484806A (zh) | 2000-07-18 | 2004-03-24 | �����弪��ϵͳ��˾ | 基于隐含模式用于从生物数据中识别生物状态的方法 |
| US20030077590A1 (en) | 2000-09-22 | 2003-04-24 | Pedersen Finn Skou | Methods for diagnosis and treatment of diseases associated with altered expression of neurogranin |
| US20030224460A1 (en) | 2000-09-22 | 2003-12-04 | Pedersen Finn Skou | Novel compositions and methods for lymphoma and leukemia |
| JP2004522980A (ja) | 2000-11-16 | 2004-07-29 | シファーゲン バイオシステムズ, インコーポレイテッド | 質量スペクトルを分析する方法 |
| US7113896B2 (en) | 2001-05-11 | 2006-09-26 | Zhen Zhang | System and methods for processing biological expression data |
| US6884591B2 (en) | 2001-06-25 | 2005-04-26 | The Cleveland Clinic Foundation | Peripheral marker of blood brain barrier permeability |
| US7144708B2 (en) | 2001-06-25 | 2006-12-05 | The Cleveland Clinic Foundation | Markers of blood barrier disruption and methods of using same |
| CA2764307C (en) | 2001-06-29 | 2015-03-03 | Meso Scale Technologies, Llc. | Assay plates, reader systems and methods for luminescence test measurements |
| WO2003068947A1 (en) | 2001-07-30 | 2003-08-21 | Meso Scale Technologies, Llc. | Assay electrodes having immobilized lipid/protein layers and methods of making and using the same |
| EP1419388B1 (en) * | 2001-08-20 | 2009-10-07 | Biosite Incorporated | Diagnostic markers of stroke and cerebral injury and methods of use thereof |
| AU2002324947B2 (en) | 2001-09-10 | 2007-11-15 | Meso Scale Technologies, Llc. | Assay buffer, compositions containing the same, and methods of using the same |
| AU2002333526B2 (en) | 2001-09-10 | 2008-01-17 | Meso Scale Technologies, Llc. | Methods and apparatus for conducting multiple measurements on a sample |
| US20070098728A1 (en) | 2001-09-24 | 2007-05-03 | Pedersen Finn S | Novel compositions and methods in cancer |
| JP4781628B2 (ja) | 2001-12-05 | 2011-09-28 | センス プロテオミック リミテッド | 対立遺伝子改変体のためのタンパク質アレイおよびその使用 |
| US20020193950A1 (en) | 2002-02-25 | 2002-12-19 | Gavin Edward J. | Method for analyzing mass spectra |
| US7036946B1 (en) | 2002-09-13 | 2006-05-02 | Rockwell Collins, Inc. | LCD backlight with UV light-emitting diodes and planar reactive element |
| EP1583950B1 (en) | 2002-12-26 | 2017-04-05 | Meso Scale Technologies, LLC. | Assay cartridges and methods of using the same |
| EP1519194A1 (en) | 2003-09-24 | 2005-03-30 | Roche Diagnostics GmbH | Use of gfap for identification of intracerebral hemorrhage |
| US7981362B2 (en) | 2003-11-04 | 2011-07-19 | Meso Scale Technologies, Llc | Modular assay plates, reader systems and methods for test measurements |
| US20140303041A1 (en) | 2004-04-15 | 2014-10-09 | University Of Florida Research Foundation Inc. | In vitro diagnostic devices for nervous system injury and other neural disorders |
| US7396654B2 (en) | 2004-04-15 | 2008-07-08 | University Of Florida Research Foundation, Inc. | Neural proteins as biomarkers for traumatic brain injury |
| US8492107B2 (en) | 2004-04-15 | 2013-07-23 | University Of Florida Research Foundation, Inc. | Neural proteins as biomarkers for nervous system injury and other neural disorders |
| US7456027B2 (en) | 2004-04-15 | 2008-11-25 | University Of Florida Research Foundation, Inc. | Proteolytic biomarkers for traumatic injury to the nervous system |
| US7776583B2 (en) | 2004-06-03 | 2010-08-17 | Meso Scale Technologies, Llc | Methods and apparatuses for conducting assays |
| WO2006012351A2 (en) | 2004-06-25 | 2006-02-02 | Washington University | Markers for brain damage |
| US20060257943A1 (en) | 2005-01-25 | 2006-11-16 | Cis Biotech, Inc. | Ischemic biomarkers and their use to predict adverse neurological events from surgery |
| US7884260B2 (en) | 2005-06-14 | 2011-02-08 | University Of Chicago | Cell-based screen for agents useful for reducing neuronal demyelination or promoting neuronal remyelination |
| GB2428240A (en) * | 2005-07-14 | 2007-01-24 | Univ Gen Ve | Diagnostic method for brain damage-related disorders |
| US20080026485A1 (en) | 2006-04-18 | 2008-01-31 | Wolfgang Hueber | Antibody profiling for determination of patient responsiveness |
| WO2007136617A2 (en) * | 2006-05-18 | 2007-11-29 | Walter Reed Army Institute Of Research (Wrair) | Endothelial-monocyte activating polypeptide ii, a biomarker for use in diagnosis of brain injury |
| ES2443042T3 (es) * | 2006-10-16 | 2014-02-17 | Bayer Intellectual Property Gmbh | LTBP2 como biomarcador, diana terapéutica y diagnóstica |
| WO2008097618A1 (en) | 2007-02-06 | 2008-08-14 | University Of Florida Research Foundation, Inc. | Synaptotagmin and collapsin response mediator protein as biomarkers for traumatic brain injury |
| EP2015196A1 (en) | 2007-06-28 | 2009-01-14 | Siemens Aktiengesellschaft | Programmer interface for manufacturing execution system |
| US8497078B2 (en) | 2008-05-23 | 2013-07-30 | The Johns Hopkins University | Biomarkers for myocardial ischemia |
| EP2143735A1 (en) | 2008-07-10 | 2010-01-13 | Institut Pasteur | Variable domains of camelid heavy-chain antibodies directed against glial fibrillary acidic proteins |
| ES2665245T3 (es) | 2008-08-11 | 2018-04-25 | Banyan Biomarkers, Inc. | Proceso de detección de biomarcador y ensayo de estado neurológico |
| US20140342381A1 (en) | 2008-08-11 | 2014-11-20 | Banyan Biomarkers, Inc. | Devices and methods for biomarker detection process and assay of neurological condition |
| US20170315136A9 (en) | 2009-06-19 | 2017-11-02 | Banyan Biomarkers, Inc. | Biomarker assay of neurological condition |
| WO2011032155A2 (en) | 2009-09-14 | 2011-03-17 | Banyan Biomarkers, Inc. | Micro-rna, autoantibody and protein markers for diagnosis of neuronal injury |
| GB201008541D0 (en) * | 2010-05-21 | 2010-07-07 | Univ Geneve | Diagnostic methods |
| US8663911B2 (en) | 2011-01-28 | 2014-03-04 | Cyrex Laboratories, Llc | Method for detection of intestinal, and blood-brain barrier permeability and testing materials thereto |
| JP2014518624A (ja) * | 2011-05-12 | 2014-08-07 | ザ・ジョンズ・ホプキンス・ユニバーシティー | ニューログラニン診断キットのためのアッセイ試薬 |
| WO2012170998A1 (en) | 2011-06-10 | 2012-12-13 | Cornell University | Immobilized protein system for rapid and enhanced multiplexed diagnostics |
| WO2013173596A1 (en) | 2012-05-16 | 2013-11-21 | Trustees Of Boston University | Chronic traumatic encephalopathy in blast-exposed individuals |
| US20150141528A1 (en) | 2013-05-31 | 2015-05-21 | Banyan Biomarkers, Inc. | Neural specific s100b for biomarker assays and devices for detection of a neurological condition |
| WO2015066211A1 (en) | 2013-10-29 | 2015-05-07 | Banyan Biomarkers, Inc. | Uch-l1 isoforms, assays and devices for detection of a neurological condition |
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| US20150051115A1 (en) | 2015-02-19 |
| IL225731B (en) | 2018-01-31 |
| WO2012051519A3 (en) | 2012-09-20 |
| US20140045713A1 (en) | 2014-02-13 |
| WO2012051519A2 (en) | 2012-04-19 |
| EP2628013B1 (en) | 2019-06-12 |
| JP2013545087A (ja) | 2013-12-19 |
| EP2628013A4 (en) | 2014-03-12 |
| EP2628013A2 (en) | 2013-08-21 |
| US9746481B2 (en) | 2017-08-29 |
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