JP6049621B2 - 分析物の分離 - Google Patents
分析物の分離 Download PDFInfo
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- JP6049621B2 JP6049621B2 JP2013530348A JP2013530348A JP6049621B2 JP 6049621 B2 JP6049621 B2 JP 6049621B2 JP 2013530348 A JP2013530348 A JP 2013530348A JP 2013530348 A JP2013530348 A JP 2013530348A JP 6049621 B2 JP6049621 B2 JP 6049621B2
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03B—SEPARATING SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS
- B03B5/00—Washing granular, powdered or lumpy materials; Wet separating
- B03B5/48—Washing granular, powdered or lumpy materials; Wet separating by mechanical classifiers
- B03B5/52—Spiral classifiers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/34—Size selective separation, e.g. size exclusion chromatography, gel filtration, permeation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6052—Construction of the column body
- G01N30/6073—Construction of the column body in open tubular form
- G01N30/6078—Capillaries
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6052—Construction of the column body
- G01N30/6086—Construction of the column body form designed to optimise dispersion
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2220/00—Aspects relating to sorbent materials
- B01J2220/80—Aspects related to sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J2220/84—Capillaries
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6095—Micromachined or nanomachined, e.g. micro- or nanosize
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Organic Chemistry (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Description
Claims (7)
- 溶離液槽と、
分析物検出器と、
液体路と、
液体を前記溶離液槽から前記液体路を通じて前記分析物検出器に送るためのポンプと、
前記液体路に試料を導入するための装置と、
前記試料導入装置の下流にある前記液体路の少なくとも一部を形成し、前記試料導入装置を通じて導入される試料を含む液体の流れが起こり得る、キャピラリーチャンネルを具え、前記キャピラリーチャンネルは、複数の細長部と複数の急な屈曲部とで構成され、前記細長部は前記急な屈曲部によって一連に相互につながれており、それぞれの前記細長部は、滑らかな線形又は曲線形を有し、
前記一連の細長部では、各一組の連続する細長部は、別々の方向に延び、且つ、前記屈曲部の1つによって相互につながり、前記液体路中にあるそれぞれの前記屈曲部は、前記ポンプが、液体を前記溶離槽から前記液体路を通じて前記分析物検出器に送っている間、その付近の液体を非層流に導くことができるほど十分に鋭く、
前記細長部のそれぞれは、上流端と下流端を有し、前記ポンプが、液体を前記溶離槽から前記液体路を通じて前記分析物検出器に送っている間、前記上流端での非層流の流れを許容し且つ消散させ、前記上流端と下流端との間で層流を再生させることができるほど十分に長く、
前記キャピラリーチャンネルは、前記急な屈曲部によって相互につながる一連の前記細長部によって構成されている螺旋形状である、
分析物の大きさに基づく分離装置。 - 前記キャピラリーチャンネルが、急な屈曲部によって相互につながる一連の細長部によって構成される細長い管からなる、請求項1の分析物の大きさに基づく分離装置。
- 前記キャピラリーチャンネルが実質的に平面上に配列された中心線を具える、請求項1の分析物の大きさに基づく分離装置。
- 前記キャピラリーチャンネルがほぼ直角の突出部を有する折れ曲りによって構成される螺旋状に形成された細長い管からなる、請求項1の分析物の大きさに基づく分離装置。
- 前記キャピラリーチャンネルが第一プレートの表面の細長い凹みと、前記表面に面するカバーによって形成される、請求項1の分析物の大きさに基づく分離装置。
- 細長いキャピラリー液体路を通って分析物検出器に大きさが異なる少なくとも2種以上の分析物を含有するサンプルを含む液体を送り、前記キャピラリー液体路が複数の細長部と複数の屈曲部を具え、前記細長部が屈曲部によって一連に相互につながり、前記細長部のそれぞれが滑らかな線形又は曲線形を有し、
前記屈曲部における前記液体の急激な方向転換によって非層流と層流の交互の領域を実現し、
前記非層流の領域において、前記キャピラリー液体路の外壁に対して小さな分析物よりも大きな分析物が近くに集められ、前記キャピラリー液体路の中心部に対して大きな分析物よりも小さな分析物が近くに集められ、
前記各細長部の長さに沿って層流となるように前記液体の流速をコントロールし、前記小さな分析物が前記大きな分析物よりも前記細長部に沿ってより速く移動し、前記大きな分析物から分離される、
分析物の大きさに基づく分離方法。 - 前記非層流領域が前記サンプルを含む液体の急激な方向転換によって実現される、請求項6の分析物の大きさに基づく分離方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US38651410P | 2010-09-26 | 2010-09-26 | |
US61/386,514 | 2010-09-26 | ||
PCT/US2011/052917 WO2012040555A1 (en) | 2010-09-26 | 2011-09-23 | Separation of analytes |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2013537980A JP2013537980A (ja) | 2013-10-07 |
JP2013537980A5 JP2013537980A5 (ja) | 2014-11-06 |
JP6049621B2 true JP6049621B2 (ja) | 2016-12-21 |
Family
ID=45874179
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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JP2013530348A Expired - Fee Related JP6049621B2 (ja) | 2010-09-26 | 2011-09-23 | 分析物の分離 |
Country Status (7)
Country | Link |
---|---|
US (1) | US9821318B2 (ja) |
EP (1) | EP2618935B1 (ja) |
JP (1) | JP6049621B2 (ja) |
CN (1) | CN103108701B (ja) |
AU (1) | AU2011305254B2 (ja) |
CA (1) | CA2810805C (ja) |
WO (1) | WO2012040555A1 (ja) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103575622B (zh) * | 2013-10-24 | 2015-11-25 | 中国石油大学(北京) | 一种蜡分子扩散系数的测试方法 |
EP3118619A1 (en) * | 2015-07-14 | 2017-01-18 | Vrije Universiteit Brussel | High-performance liquid chromatography with a vortex flow inducer |
JP2018089576A (ja) * | 2016-12-02 | 2018-06-14 | 東ソー株式会社 | 粒子分離装置 |
CN106596692B (zh) * | 2017-01-14 | 2018-12-28 | 常州大学 | 一种弯曲毛细管电泳检测抗体多肽相互作用的方法 |
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US5032283A (en) * | 1983-03-03 | 1991-07-16 | The Perkin Elmer Corporation | Low dispersion fluid conduit useful in chromatography systems |
EP0339780B1 (en) * | 1988-04-29 | 1996-08-28 | Beckman Instruments, Inc. | Capillary detector cartridge for electrophoresis |
WO1996010170A1 (en) * | 1994-09-29 | 1996-04-04 | The Board Of Trustees Of The Leland Stanford Junior University | Capillary-based separation methods for identifying bioactive analytes in a mixture |
US5716852A (en) * | 1996-03-29 | 1998-02-10 | University Of Washington | Microfabricated diffusion-based chemical sensor |
US5772874A (en) * | 1995-11-02 | 1998-06-30 | Cohesive Technologies, Inc. | High performance liquid chromatography method and apparatus |
US5948684A (en) * | 1997-03-31 | 1999-09-07 | University Of Washington | Simultaneous analyte determination and reference balancing in reference T-sensor devices |
JP3469585B2 (ja) * | 1997-05-23 | 2003-11-25 | ガメラ バイオサイエンス コーポレイション | ミクロ流体工学システムでの流動運動を駆動するために向心的加速を使用するための装置および方法 |
JP2001523001A (ja) * | 1997-11-12 | 2001-11-20 | ピーイー コーポレイション (エヌワイ) | 自己修正ベンドをもつ蛇行電気泳動チャネル |
WO1999060397A1 (en) | 1998-05-18 | 1999-11-25 | University Of Washington | Liquid analysis cartridge |
EP1125105A2 (en) * | 1998-11-05 | 2001-08-22 | ChemoMetec A/S | A method for the assessment of particles and a system and a device for use in the method |
JP2004509335A (ja) * | 2000-09-18 | 2004-03-25 | ユニバーシティ オブ ワシントン | 複数のフローストリーム間の流体界面の回転操作のための微小流体デバイス |
US20040224425A1 (en) * | 2003-05-08 | 2004-11-11 | Gjerde Douglas T. | Biomolecule open channel solid phase extraction systems and methods |
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JP2006090806A (ja) * | 2004-09-22 | 2006-04-06 | Yamatake Corp | クロマトグラフ装置 |
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CA2505657A1 (en) * | 2005-04-28 | 2006-10-28 | York University | Method for mixing inside a capillary and device for achieving same |
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SE529516C2 (sv) * | 2005-10-24 | 2007-09-04 | Alfa Laval Corp Ab | Universell flödesmodul |
US20070122819A1 (en) * | 2005-11-25 | 2007-05-31 | Industrial Technology Research Institute | Analyte assay structure in microfluidic chip for quantitative analysis and method for using the same |
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WO2009002580A2 (en) * | 2007-04-02 | 2008-12-31 | Boston Medical Center Corporation | Method for bacterial lysis |
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JP5413916B2 (ja) * | 2007-09-19 | 2014-02-12 | オプコ・ダイアグノスティクス・リミテッド・ライアビリティ・カンパニー | 統合検定のための液体格納 |
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-
2011
- 2011-09-23 US US13/825,077 patent/US9821318B2/en active Active
- 2011-09-23 EP EP11827594.0A patent/EP2618935B1/en not_active Not-in-force
- 2011-09-23 CA CA2810805A patent/CA2810805C/en not_active Expired - Fee Related
- 2011-09-23 WO PCT/US2011/052917 patent/WO2012040555A1/en active Application Filing
- 2011-09-23 JP JP2013530348A patent/JP6049621B2/ja not_active Expired - Fee Related
- 2011-09-23 CN CN201180044834.7A patent/CN103108701B/zh not_active Expired - Fee Related
- 2011-09-23 AU AU2011305254A patent/AU2011305254B2/en not_active Ceased
Also Published As
Publication number | Publication date |
---|---|
EP2618935B1 (en) | 2018-11-07 |
CA2810805A1 (en) | 2012-03-29 |
EP2618935A1 (en) | 2013-07-31 |
CA2810805C (en) | 2014-11-18 |
CN103108701B (zh) | 2015-03-11 |
WO2012040555A1 (en) | 2012-03-29 |
AU2011305254A1 (en) | 2013-03-14 |
US9821318B2 (en) | 2017-11-21 |
US20130180894A1 (en) | 2013-07-18 |
CN103108701A (zh) | 2013-05-15 |
EP2618935A4 (en) | 2015-10-28 |
JP2013537980A (ja) | 2013-10-07 |
AU2011305254B2 (en) | 2014-06-05 |
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