JP6029651B2 - ゲル浸透クロマトグラフィーを使用してウイルスを精製する方法 - Google Patents

ゲル浸透クロマトグラフィーを使用してウイルスを精製する方法 Download PDF

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JP6029651B2
JP6029651B2 JP2014506699A JP2014506699A JP6029651B2 JP 6029651 B2 JP6029651 B2 JP 6029651B2 JP 2014506699 A JP2014506699 A JP 2014506699A JP 2014506699 A JP2014506699 A JP 2014506699A JP 6029651 B2 JP6029651 B2 JP 6029651B2
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virus
elution buffer
gel permeation
buffered saline
permeation chromatography
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JP2014516515A (ja
JP2014516515A5 (cg-RX-API-DMAC10.html
Inventor
コフィー，　マシュー　シー．
マシュー シー． コフィー，
アリソン ヘイゲルマン，
アリソン ヘイゲルマン，
ロクソナ カパディア，
ロクソナ カパディア，
サラ セル，
サラ セル，
Original Assignee
オンコリティクス バイオテク，インコーポレーテッド
オンコリティクス バイオテク，インコーポレーテッド
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C12N2720/12051—Methods of production or purification of viral material

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicinal Preparation (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
JP2014506699A 2011-04-29 2012-04-27 ゲル浸透クロマトグラフィーを使用してウイルスを精製する方法 Active JP6029651B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161480561P 2011-04-29 2011-04-29
US61/480,561 2011-04-29
PCT/CA2012/000406 WO2012145837A1 (en) 2011-04-29 2012-04-27 Methods of purifying viruses using gel permeation chromatography

Publications (3)

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JP2014516515A JP2014516515A (ja) 2014-07-17
JP2014516515A5 JP2014516515A5 (cg-RX-API-DMAC10.html) 2015-06-18
JP6029651B2 true JP6029651B2 (ja) 2016-11-24

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Country Link
US (1) US20120273424A1 (cg-RX-API-DMAC10.html)
EP (1) EP2702147B1 (cg-RX-API-DMAC10.html)
JP (1) JP6029651B2 (cg-RX-API-DMAC10.html)
KR (2) KR20140024406A (cg-RX-API-DMAC10.html)
CN (1) CN103492557B (cg-RX-API-DMAC10.html)
AU (1) AU2012248082B2 (cg-RX-API-DMAC10.html)
CA (1) CA2832367A1 (cg-RX-API-DMAC10.html)
DK (1) DK2702147T3 (cg-RX-API-DMAC10.html)
EA (1) EA201391605A1 (cg-RX-API-DMAC10.html)
ES (1) ES2822199T3 (cg-RX-API-DMAC10.html)
IL (1) IL228491A (cg-RX-API-DMAC10.html)
MX (1) MX337348B (cg-RX-API-DMAC10.html)
SG (1) SG194449A1 (cg-RX-API-DMAC10.html)
TW (1) TW201307561A (cg-RX-API-DMAC10.html)
WO (1) WO2012145837A1 (cg-RX-API-DMAC10.html)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2819236A1 (en) 2010-12-02 2012-06-07 Oncolytics Biotech Inc. Lyophilized viral formulations
MX373096B (es) * 2010-12-02 2020-04-27 Oncolytics Biotech Inc Formulaciones virales líquidas.
RS57864B1 (sr) * 2013-06-17 2018-12-31 De Staat Der Nederlanden Vert Door De Minister Van Vws Ministerie Van Volksgezondheid Welzijn En Spo Postupci za sprečavanje agregacije virusnih komponenti
CA3104392A1 (en) 2018-07-04 2020-01-09 Probiogen Ag Method for purifying an enveloped virus
CN111812313B (zh) * 2020-06-22 2021-10-08 北京生物制品研究所有限责任公司 铝佐剂吸附型新型冠状病毒灭活疫苗中抗原的解离方法
CN114544815B (zh) * 2022-03-01 2023-10-27 中牧实业股份有限公司 一种山羊痘病毒的定量检测方法
CN116718524B (zh) * 2023-06-26 2025-11-07 西安交通大学 一种基于细胞膜色谱技术的病毒颗粒检测方法
HUP2400154A1 (hu) * 2024-02-29 2025-09-28 Pecsi Tudomanyegyetem Elúciós oldat és tisztítási eljárás

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5096885A (en) * 1988-04-15 1992-03-17 Genentech, Inc. Human growth hormone formulation
DE69029765T2 (de) * 1989-07-24 1997-05-15 Bayer Ag Stabilisierung von hochgereinigten Proteinen
ES2264126T3 (es) * 1994-05-16 2006-12-16 MERCK & CO., INC. Vacunas contra el papilomavirus.
KR100918187B1 (ko) * 1998-02-17 2009-09-22 쉐링 코포레이션 바이러스를 포함하는 조성물 및 바이러스 제제의 농축방법
IL157304A0 (en) 2001-03-16 2004-02-19 Oncolytics Biotech Inc Method of extracting virus from cell culture
DE60327069D1 (de) * 2002-04-30 2009-05-20 Oncolytics Biotech Inc Verbesserte reinigungsmethode für viren
US7901921B2 (en) * 2004-10-22 2011-03-08 Oncolytics Biotech Inc. Viral purification methods
EP1878791A1 (en) * 2006-07-11 2008-01-16 Bia Separations D.O.O. Method for influenza virus purification
CA2662569C (en) * 2006-08-07 2017-01-03 Japan As Represented By Director General Of National Institute Of Infectious Diseases Process for preparing live smallpox vaccine
SG191602A1 (en) * 2007-03-12 2013-07-31 Oncolytics Biotech Inc Reoviruses having modified sequences
AU2008321016B2 (en) * 2007-11-12 2013-03-28 Theraclone Sciences, Inc. Compositions and methods for the therapy and diagnosis of influenza
US8227577B2 (en) * 2007-12-21 2012-07-24 Hoffman-La Roche Inc. Bivalent, bispecific antibodies
CA2819236A1 (en) * 2010-12-02 2012-06-07 Oncolytics Biotech Inc. Lyophilized viral formulations
MX373096B (es) * 2010-12-02 2020-04-27 Oncolytics Biotech Inc Formulaciones virales líquidas.

Also Published As

Publication number Publication date
KR20190026059A (ko) 2019-03-12
AU2012248082A1 (en) 2013-05-09
EP2702147A1 (en) 2014-03-05
CA2832367A1 (en) 2012-11-01
EA201391605A1 (ru) 2014-02-28
CN103492557B (zh) 2015-07-29
IL228491A0 (en) 2013-12-31
SG194449A1 (en) 2013-12-30
WO2012145837A1 (en) 2012-11-01
CN103492557A (zh) 2014-01-01
DK2702147T3 (da) 2020-09-28
IL228491A (en) 2017-05-29
JP2014516515A (ja) 2014-07-17
ES2822199T3 (es) 2021-04-29
MX2013012627A (es) 2014-05-13
AU2012248082B2 (en) 2015-08-20
KR102151890B1 (ko) 2020-09-03
TW201307561A (zh) 2013-02-16
KR20140024406A (ko) 2014-02-28
US20120273424A1 (en) 2012-11-01
EP2702147B1 (en) 2020-07-15
MX337348B (es) 2016-02-29
EP2702147A4 (en) 2014-11-05

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