CA2832367A1 - Methods of purifying viruses using gel permeation chromatography - Google Patents

Methods of purifying viruses using gel permeation chromatography Download PDF

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Publication number
CA2832367A1
CA2832367A1 CA2832367A CA2832367A CA2832367A1 CA 2832367 A1 CA2832367 A1 CA 2832367A1 CA 2832367 A CA2832367 A CA 2832367A CA 2832367 A CA2832367 A CA 2832367A CA 2832367 A1 CA2832367 A1 CA 2832367A1
Authority
CA
Canada
Prior art keywords
virus
elution buffer
gel permeation
permeation chromatography
buffered saline
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2832367A
Other languages
English (en)
French (fr)
Inventor
Roxna KAPADIA
Matthew C. Coffey
Allison HAGERMAN
Sarah Serl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oncolytics Biotech Inc
Original Assignee
Oncolytics Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oncolytics Biotech Inc filed Critical Oncolytics Biotech Inc
Publication of CA2832367A1 publication Critical patent/CA2832367A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C12N2720/12051—Methods of production or purification of viral material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicinal Preparation (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
CA2832367A 2011-04-29 2012-04-27 Methods of purifying viruses using gel permeation chromatography Abandoned CA2832367A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161480561P 2011-04-29 2011-04-29
US61/480,561 2011-04-29
PCT/CA2012/000406 WO2012145837A1 (en) 2011-04-29 2012-04-27 Methods of purifying viruses using gel permeation chromatography

Publications (1)

Publication Number Publication Date
CA2832367A1 true CA2832367A1 (en) 2012-11-01

Family

ID=47067091

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2832367A Abandoned CA2832367A1 (en) 2011-04-29 2012-04-27 Methods of purifying viruses using gel permeation chromatography

Country Status (15)

Country Link
US (1) US20120273424A1 (cg-RX-API-DMAC10.html)
EP (1) EP2702147B1 (cg-RX-API-DMAC10.html)
JP (1) JP6029651B2 (cg-RX-API-DMAC10.html)
KR (2) KR20140024406A (cg-RX-API-DMAC10.html)
CN (1) CN103492557B (cg-RX-API-DMAC10.html)
AU (1) AU2012248082B2 (cg-RX-API-DMAC10.html)
CA (1) CA2832367A1 (cg-RX-API-DMAC10.html)
DK (1) DK2702147T3 (cg-RX-API-DMAC10.html)
EA (1) EA201391605A1 (cg-RX-API-DMAC10.html)
ES (1) ES2822199T3 (cg-RX-API-DMAC10.html)
IL (1) IL228491A (cg-RX-API-DMAC10.html)
MX (1) MX337348B (cg-RX-API-DMAC10.html)
SG (1) SG194449A1 (cg-RX-API-DMAC10.html)
TW (1) TW201307561A (cg-RX-API-DMAC10.html)
WO (1) WO2012145837A1 (cg-RX-API-DMAC10.html)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025181504A1 (en) * 2024-02-29 2025-09-04 Pécsi Tudományegyetem Purification of infective virus

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2819236A1 (en) 2010-12-02 2012-06-07 Oncolytics Biotech Inc. Lyophilized viral formulations
MX373096B (es) * 2010-12-02 2020-04-27 Oncolytics Biotech Inc Formulaciones virales líquidas.
RS57864B1 (sr) * 2013-06-17 2018-12-31 De Staat Der Nederlanden Vert Door De Minister Van Vws Ministerie Van Volksgezondheid Welzijn En Spo Postupci za sprečavanje agregacije virusnih komponenti
CA3104392A1 (en) 2018-07-04 2020-01-09 Probiogen Ag Method for purifying an enveloped virus
CN111812313B (zh) * 2020-06-22 2021-10-08 北京生物制品研究所有限责任公司 铝佐剂吸附型新型冠状病毒灭活疫苗中抗原的解离方法
CN114544815B (zh) * 2022-03-01 2023-10-27 中牧实业股份有限公司 一种山羊痘病毒的定量检测方法
CN116718524B (zh) * 2023-06-26 2025-11-07 西安交通大学 一种基于细胞膜色谱技术的病毒颗粒检测方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5096885A (en) * 1988-04-15 1992-03-17 Genentech, Inc. Human growth hormone formulation
DE69029765T2 (de) * 1989-07-24 1997-05-15 Bayer Ag Stabilisierung von hochgereinigten Proteinen
ES2264126T3 (es) * 1994-05-16 2006-12-16 MERCK & CO., INC. Vacunas contra el papilomavirus.
KR100918187B1 (ko) * 1998-02-17 2009-09-22 쉐링 코포레이션 바이러스를 포함하는 조성물 및 바이러스 제제의 농축방법
IL157304A0 (en) 2001-03-16 2004-02-19 Oncolytics Biotech Inc Method of extracting virus from cell culture
DE60327069D1 (de) * 2002-04-30 2009-05-20 Oncolytics Biotech Inc Verbesserte reinigungsmethode für viren
US7901921B2 (en) * 2004-10-22 2011-03-08 Oncolytics Biotech Inc. Viral purification methods
EP1878791A1 (en) * 2006-07-11 2008-01-16 Bia Separations D.O.O. Method for influenza virus purification
CA2662569C (en) * 2006-08-07 2017-01-03 Japan As Represented By Director General Of National Institute Of Infectious Diseases Process for preparing live smallpox vaccine
SG191602A1 (en) * 2007-03-12 2013-07-31 Oncolytics Biotech Inc Reoviruses having modified sequences
AU2008321016B2 (en) * 2007-11-12 2013-03-28 Theraclone Sciences, Inc. Compositions and methods for the therapy and diagnosis of influenza
US8227577B2 (en) * 2007-12-21 2012-07-24 Hoffman-La Roche Inc. Bivalent, bispecific antibodies
CA2819236A1 (en) * 2010-12-02 2012-06-07 Oncolytics Biotech Inc. Lyophilized viral formulations
MX373096B (es) * 2010-12-02 2020-04-27 Oncolytics Biotech Inc Formulaciones virales líquidas.

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025181504A1 (en) * 2024-02-29 2025-09-04 Pécsi Tudományegyetem Purification of infective virus

Also Published As

Publication number Publication date
KR20190026059A (ko) 2019-03-12
AU2012248082A1 (en) 2013-05-09
EP2702147A1 (en) 2014-03-05
EA201391605A1 (ru) 2014-02-28
CN103492557B (zh) 2015-07-29
IL228491A0 (en) 2013-12-31
SG194449A1 (en) 2013-12-30
WO2012145837A1 (en) 2012-11-01
CN103492557A (zh) 2014-01-01
DK2702147T3 (da) 2020-09-28
IL228491A (en) 2017-05-29
JP2014516515A (ja) 2014-07-17
ES2822199T3 (es) 2021-04-29
MX2013012627A (es) 2014-05-13
AU2012248082B2 (en) 2015-08-20
KR102151890B1 (ko) 2020-09-03
TW201307561A (zh) 2013-02-16
KR20140024406A (ko) 2014-02-28
US20120273424A1 (en) 2012-11-01
EP2702147B1 (en) 2020-07-15
MX337348B (es) 2016-02-29
EP2702147A4 (en) 2014-11-05
JP6029651B2 (ja) 2016-11-24

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20170424

FZDE Discontinued

Effective date: 20201007