JP5982039B2 - Indicator for body odor determination - Google Patents

Description

  The present invention relates to an indicator for body odor determination. More specifically, the present invention relates to an indicator for body odor determination useful for development of cosmetics, fragrances, cleaning agents, etc. suitable for humans who care about body odor, evaluation method for deodorizing effect of body odor, evaluation of deodorant agent The present invention relates to a method, a method for determining body odor, and a method for screening a body odor inhibitor.

  The body odor is a mixture of a plurality of odors generated from each part such as the axilla, head, trunk, toe, and sole. Among these parts, for example, the axilla and the head are parts where the amount of sweat secretion is large, but the sweat is hard to evaporate, and the environment where the skin resident microorganisms are easy to propagate. In such an axilla and head, components contained in sweat are decomposed by skin resident microorganisms together with sebum, dirt, etc., and a substance that emits a strong odor is generated. Therefore, the smell of the axilla (hereinafter referred to as “smelling odor”) and the smell of the head (hereinafter referred to as “head odor”) are strong and easily perceived by others.

  In recent years, the desire for cleanliness is increasing, and there is a tendency for more people to care about their body odors. Therefore, cosmetics, fragrances, cleaning agents, and the like that exhibit a deodorizing effect on body odors have been proposed.

  The deodorizing effect of body odor due to cosmetics, fragrances, cleaning agents, etc. is often a method that allows the panel to evaluate the presence or absence of body odors on the monitor after using the monitor with cosmetics, fragrances, cleaning agents, etc. Is evaluated by a method in which a panelist evaluates the presence or absence of body odor attached to clothes after applying cosmetics, fragrances, cleaning agents, or the like to the clothes used. However, since these methods are easily affected by the physical condition of the monitor and the panelist, it is difficult to objectively and accurately evaluate the deodorizing effect of the body odor.

  On the other hand, it is conceivable to use a simulated body odor using a causative substance of body odor for evaluating the deodorizing effect of body odor.

  For example, as a causative substance of a bad odor, a fatty acid having 2 to 11 carbon atoms such as 3-methyl-2-hexenoic acid, 4-ethylhexanoic acid, 7-octenoic acid; androstenone (5α-16-androsten-3-one ), Androstenol (such as 5α-16-androsten-3α-ol or 5α-16-androsten-3β-ol), androstadienone (4,16-androstadien-3-one), etc. Is known (see, for example, Patent Document 1 and Non-Patent Document 1). Among these, carboxylic acids having 2 to 5 carbon atoms are considered to be a causative substance of acid odor, which has a strong odor and an unpleasant odor. However, the smell of the pseudo odor composition containing the causative substance does not sufficiently reproduce the odor and is different from the actual odor.

  Further, as a causative substance of head odor, for example, fatty acids such as acetic acid and propionic acid are known (for example, see Patent Document 2). However, the odor of the simulated head odor composition containing the causative substance does not sufficiently reproduce the head odor and is different from the actual head odor.

  Therefore, objectively and accurately evaluate indicators that can reproduce human body odor accurately and objectively evaluate it, as well as deodorizing effects of body odors such as cosmetics, fragrances, and detergents, etc. with simple operations. There is a need for means that can

JP 2005-62159 A JP 2001-220593 A

The Chemical Society of Japan, “Molecule Recognition of Taste and Odor”, Quarterly Chemical Review, Society Publishing Center, March 1999, Volume 40, p. 205-211

  This invention is made | formed in view of the said prior art, and it aims at providing the body odor determination index agent which can reproduce a human body odor accurately and can objectively evaluate. Another object of the present invention is to provide a method for evaluating the deodorizing effect of body odor that can objectively and accurately evaluate the deodorizing effect of body odor with a simple operation. Furthermore, an object of this invention is to provide the evaluation method of a deodorant agent which can objectively and accurately evaluate the performance of a deodorant agent by simple operation. Another object of the present invention is to provide a method for determining body odor that can objectively and accurately determine the type and intensity of body odor with a simple operation. An object of this invention is to provide the screening method of the body odor suppressor which can screen a body odor suppressor efficiently by simple operation.

That is, the gist of the present invention is as follows.
(1) An indicator for body odor determination for use as an index for determination of body odor, wherein the general formula (I):
R ^1- (CO)-(CO) -R ² (I)
(Wherein R ¹ and R ² each independently represents an alkyl group having 1 to 4 carbon atoms)
An indicator for body odor determination, comprising a diketone compound represented by:
(2) The indicator for body odor determination according to (1), further comprising a fatty acid having 2 to 5 carbon atoms,
(3) The indicator for body odor determination according to (1), further comprising a fatty acid having 6 to 10 carbon atoms,
(4) The indicator for body odor determination according to (3), further comprising nonenal,
(5) After contacting the test sample with the indicator for body odor determination according to any one of (1) to (4), the presence or absence of an odor emitted by the indicator for body odor determination, the intensity of the odor, and the above At least one selected from the group consisting of odor discomfort is examined, and from the difference in odor intensity due to the presence or absence of odor, the presence or absence of contact with the test sample, and the difference in odor discomfort due to the presence or absence of contact with the test sample A method for evaluating the deodorizing effect of body odor, wherein the deodorizing effect of body odor is evaluated by at least one selected from the group consisting of:
(6) Collecting substances present at the application site of the deodorant agent, examining the amount of the indicator for body odor determination according to any one of (1) to (4) contained in the substance, and determining the body odor A method of evaluating a deodorant agent, characterized by evaluating the performance of the deodorant agent according to the amount of the indicator agent;
(7) A substance present on the skin is collected, the amount of the body odor determination index agent according to any one of (1) to (4) contained in the obtained substance is examined, and the body odor determination index Body odor determination method, characterized by determining body odor type or body odor intensity according to the amount of the agent,
(8) A screening method for a body odor suppressor for suppressing the generation of body odor caused by the indicator for body odor determination according to any one of (1) to (4),
(A) General formula (I):
R ^1- (CO)-(CO) -R ² (I)
(Wherein R ¹ and R ² each independently represents an alkyl group having 1 to 4 carbon atoms)
Culturing a microorganism capable of producing a diketone compound represented by a medium containing a test substance and a precursor of the diketone compound,
(B) By measuring the number of viable microorganisms and / or the amount of the diketone compound in the culture obtained in the step (A), it is determined whether the test substance suppresses the production of the diketone compound. And (C) a screening method for a body odor suppressor, comprising the step of selecting, as a body odor suppressor, a test substance that suppresses the formation of a diketone compound in step (B), and (9) the diketone The screening method according to (8) above, wherein the microorganism capable of producing a compound is at least one selected from the group consisting of Staphylococcus aureus and Staphylococcus epidermidis.

  The indicator for body odor determination of the present invention has an excellent effect of accurately reproducing human body odor and objectively evaluating it. Moreover, the evaluation method of the deodorizing effect of body odor of this invention has the outstanding effect that the deodorizing effect of body odor can be objectively and accurately evaluated by simple operation. Furthermore, the deodorant agent evaluation method of the present invention has an excellent effect that the performance of the deodorant agent can be objectively and accurately evaluated by a simple operation. Further, the body odor determination method of the present invention has an excellent effect that the type and intensity of body odor can be determined objectively and accurately by a simple operation. The screening method for a body odor suppressor of the present invention has an excellent effect that the body odor suppressor can be efficiently screened by a simple operation.

(A) is the graph which shows the result which investigated the relationship between the site | part of a head and the ratio of the sweat odor which occupies for head odor in Experimental example 1, (B) is a head part and head odor in Experimental example 1. It is a graph which shows the result of having investigated the relationship with the ratio of the oil odor to occupy. In Example 1, it is the chromatogram which shows the result of having analyzed the component adhering to the pillow cover used by the test subject who has an oil odor. In Example 1, it is a graph which shows the result of having investigated the relationship between the presence or absence of an oil odor, and the area of the peak corresponding to diacetyl. In Example 1, it is a graph which shows the result of having investigated the quantity of each compound detected by the gas chromatograph-mass spectrometry. (A) is the chromatogram which shows the result of having analyzed the component contained in the headspace gas of the test subject who has strong oil odor in the scalp in Example 2, and (B) is the test subject in Example 2 who has weak oil odor in the scalp. It is a chromatogram which shows the result of having analyzed the component contained in headspace gas of. In Example 3, it is a graph which shows the result of having investigated the relationship between the oil odor level and the area of the peak corresponding to diacetyl. In Example 4, it is a graph which shows the result of having investigated the relationship between the level of a head odor, and the density | concentration of the diacetyl in the head space gas of a head. In Example 4, it is a graph which shows the result of having investigated the relationship between the level of an oil odor, and the density | concentration of the diacetyl in the head space gas of a head. (A) is the graph which shows the result which investigated the relationship between the type of odor and the area of the peak corresponding to diacetyl in Example 5, (B) is the peak of the peak corresponding to the type of odor and acetic acid in Example 5. It is a graph which shows the result of having investigated the relationship with an area. (A) is the chromatogram which shows the result of having analyzed the component adhering to the cotton sheet | seat contacted with the armpit part of the test subject with a strong acid odor in Example 6, (B) is a test subject's axilla part with a weak acid odor. It is a chromatogram which shows the result of having analyzed the component adhering to the contacted cotton sheet. (A) is the graph which shows the result of having investigated the relationship between the concentration of pyruvic acid in a culture, and the kind of Staphylococcus genus bacteria in Experimental example 3, (B) is the density | concentration of diacetyl in a culture in Experimental example 3. It is a graph which shows the result of having investigated the relationship between and the kind of Staphylococcus genus bacteria. (A) is the graph which shows the result which investigated the relationship between the concentration of pyruvic acid in a culture, and the kind of Corynebacterium in Experimental example 3, (B) is the density | concentration of diacetyl in a culture in Experimental example 3. It is a graph which shows the result of having investigated the relationship between and the kind of Corynebacterium genus bacteria. In Experimental example 4, it is a graph which shows the result of having investigated the relationship between the kind of carbon source, and the density | concentration of the diacetyl in a culture. In Example 19, it is a graph which shows the result of having investigated the relationship between the kind of test substance, and inhibition rate.

The body odor determination indicator of the present invention, as described above, is a body odor determination indicator for use as a body odor determination index,
Formula (I):
R ^1- (CO)-(CO) -R ² (I)
(Wherein R ¹ and R ² each independently represents an alkyl group having 1 to 4 carbon atoms)
It contains the diketone compound represented by these.

  Since the indicator for body odor determination of the present invention contains the diketone compound, it can accurately reproduce the human body odor, particularly the oily odor in the head and the acid odor in the armpit. Therefore, the body odor determination indicator of the present invention is useful for evaluating body odor deodorizing effect or deodorizing effect, human body odor type, body odor intensity, deodorant agent performance, and the like.

  In the present specification, “body odor” refers to an odor generated from the surface of the skin. In the present specification, “head odor” refers to an odor generated from the head including scalp and hair among body odors.

  Further, in this specification, the “oil odor” is similar to the scent of old oil and refers to a smell that is fermented. Further, in the present specification, the “acid odor” refers to a sour smell that is steamed.

  Further, in this specification, the concept of “deodorizing body odor” includes masking body odor, removing the body odor causing substance by decomposition, absorption, adsorption, washing, wiping, etc. It includes changing the structure to remove unpleasant odors and suppressing the volatilization of the body odor-causing substances.

  In the present specification, “deodorizing body odor” refers to suppressing the generation of body odor.

In the general formula (I), R ¹ and R ² are each independently an alkyl group having 1 to 4 carbon atoms. The alkyl group has 1 to 4 carbon atoms, preferably 1 to 3, more preferably 1 or 2, and still more preferably 1. Examples of the alkyl group having 1 to 4 carbon atoms include a methyl group, an ethyl group, an n-propyl group, an isopropyl group, an n-butyl group, an isobutyl group, and a tert-butyl group. It is not limited to illustration only. Among these, a methyl group is preferable from the viewpoint of accurately reproducing human body odor.

  Examples of the diketone compound include diacetyl, 2,3-pentanedione, and 2,3-hexanedione, but the present invention is not limited to such examples. Among the diketone compounds, diacetyl and 2,3-pentanedione are preferable, and diacetyl is more preferable because human body odors, particularly the odor of abra in the head and the acid odor in the axilla, can be accurately reproduced. The diketone compounds represented by the general formula (I) can be used alone or in admixture of two or more.

  Since the content of the diketone compound in the body odor determination indicator of the present invention varies depending on the use of the body odor determination indicator, etc., it cannot be generally determined. Therefore, it is desirable that the content is appropriately determined according to the use of the indicator for body odor determination.

  The content rate of the diketone compound in the indicator for body odor determination of the present invention, for example, when used to evaluate the deodorizing effect of body odor, more accurately evaluate the difference in odor intensity, and accurately reproduce the characteristics of odor From this point of view, it is preferably 0.0001 to 0.1 mass%, more preferably 0.001 to 0.05 mass%. The content rate of the diketone compound in the indicator for body odor determination of the present invention can be easily adjusted with a diluent or the like at the time of use. Moreover, the content rate of the diketone compound in the index agent for body odor determination of this invention may be adjusted previously. Examples of the diluent include mineral oil, propylene glycol, 1.3-butylene glycol, and the like, but the present invention is not limited to such examples.

  The indicator for body odor determination of the present invention, for example, when used for determination of body odor or evaluation of a deodorant agent, may be composed of only a diketone compound, and a mixture of the diketone compound and a component according to the type of body odor. It may be.

  When the body odor is a musty odor, the indicator for body odor determination of the present invention further contains a fatty acid having 2 to 5 carbon atoms as a component according to the type of the body odor, from the viewpoint of accurately reproducing the human odor. It is preferable. The number of carbon atoms of the fatty acid is 2 to 5, preferably 2 or 3, from the viewpoint of accurately reproducing the odor. Examples of the fatty acid having 2 to 5 carbon atoms include acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, and isovaleric acid, but the present invention is not limited to such examples. Among these, acetic acid and propionic acid are preferable from the viewpoint of accurately reproducing the human odor. These C2-5 fatty acids can be used alone or in admixture of two or more.

  In the indicator for body odor determination of the present invention, the amount of the fatty acid having 2 to 5 carbon atoms per 100 parts by mass of the diketone compound varies depending on the type of fatty acid, the olfactory threshold of each fatty acid, odor characteristics, etc. Cannot be determined. Usually, the amount of the fatty acid having 2 to 5 carbon atoms per 100 parts by mass of the diketone compound is preferably 1 to 10000 parts by mass, more preferably 10 to 1000 parts by mass, from the viewpoint of accurately reproducing the human odor. is there.

  When the body odor is a bad odor, the body odor determination indicator of the present invention may contain other components. Examples of other components include fatty acids other than fatty acids having 2 to 5 carbon atoms, diluents, and aldehydes having 1 to 10 carbon atoms, but the present invention is not limited to such examples.

  Examples of the fatty acid other than the fatty acid having 2 to 5 carbon atoms include fatty acids having 6 to 10 carbon atoms such as caproic acid, enanthic acid, caprylic acid, pelargonic acid, capric acid, and the like. It is not limited to illustration only.

  Examples of the aldehyde having 1 to 10 carbon atoms include saturated aldehydes such as formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, valeric aldehyde, hexanal, heptanal, octanal, nonanal and decanal; unsaturated such as octenal, nonenal and desenal. Although aldehyde etc. are mentioned, this invention is not limited only to this illustration.

  When the indicator for body odor determination of the present invention contains the other component, in the indicator for body odor determination, the amount of the other component per 100 parts by mass of the diketone compound depends on the type of the other component, etc. Because it is different, it cannot be determined in general. Usually, the amount of the other component per 100 parts by mass of the diketone compound is preferably 1 to 1000000 parts by mass, more preferably 10 to 100000 parts by mass, and even more preferably 30 to 30 parts from the viewpoint of accurately reproducing the human odor. 10,000, even more preferably 50-1000.

  When the body odor is a head odor, the indicator for body odor determination of the present invention has 6 to 10 carbon atoms as a component according to the type of the body odor from the viewpoint of more accurately reproducing the human head odor. It is preferable to further contain a fatty acid.

  The number of carbon atoms of the fatty acid is 6 to 10, preferably 7 to 10 from the viewpoint of accurately reproducing the human head odor. Examples of the fatty acid having 6 to 10 carbon atoms include caproic acid, isocaproic acid, enanthic acid, isoenanthic acid, caprylic acid, isocaprylic acid, pelargonic acid, isopelargonic acid, capric acid, and isocaric acid. The invention is not limited to such examples. Among these, enanthic acid, caprylic acid, pelargonic acid and capric acid are preferred from the viewpoint of accurately reproducing the human head odor. These fatty acids having 6 to 10 carbon atoms can be used alone or in admixture of two or more.

  In the indicator for body odor determination of the present invention, the amount of the fatty acid having 6 to 10 carbon atoms per 100 parts by mass of the diketone compound varies depending on the type of fatty acid, the olfactory threshold of each fatty acid, odor characteristics, etc. Cannot be determined. In general, the amount of the fatty acid having 6 to 10 carbon atoms per 100 parts by mass of the diketone compound is preferably 10 to 40000 parts by mass, more preferably 30 to 8000 parts by mass, from the viewpoint of accurately reproducing the human odor. More preferably, it is 50-5000.

  When the body odor is a head odor, the indicator for body odor determination of the present invention preferably further contains Nonenal from the viewpoint of more accurately reproducing the human head odor.

  In the indicator for body odor determination according to the present invention, the amount of the nonenal per 100 parts by mass of the diketone compound varies depending on the characteristics of the target odor and cannot be determined unconditionally. Usually, the amount of the nonenal per 100 parts by mass of the diketone compound is preferably 0.01 to 1000 parts by mass, more preferably 0.05 to 500 parts by mass, from the viewpoint of more accurately reproducing the human odor. More preferably, it is 0.1-300 mass parts.

  When the body odor is a head odor, the body odor determination indicator of the present invention may contain other components as long as the object of the present invention is not impaired. Examples of other components include fatty acids other than fatty acids having 6 to 10 carbon atoms, diluents, and aldehydes having 1 to 10 carbon atoms, but the present invention is not limited to such examples.

  Examples of fatty acids other than the fatty acids having 6 to 10 carbon atoms include fatty acids having 2 to 5 carbon atoms such as acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, and isovaleric acid; undecanoic acid, dodecanoic acid, and tridecanoic acid And fatty acids having 11 to 18 carbon atoms such as myristic acid, palmitic acid, stearic acid, palmitoleic acid, and oleic acid, but the present invention is not limited to such examples.

  Examples of the aldehyde having 1 to 10 carbon atoms include saturated aldehydes such as formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, valeric aldehyde, hexanal, heptanal, octanal, nonanal and decanal; unsaturated such as octenal, nonenal and desenal. Although aldehyde etc. are mentioned, this invention is not limited only to this illustration.

  When the indicator for body odor determination of the present invention contains the other component, in the indicator for body odor determination, the amount of the other component per 100 parts by mass of the diketone compound depends on the type of the other component, etc. Because it is different, it cannot be determined in general. Usually, the amount of the other component per 100 parts by mass of the diketone compound is preferably 1 to 1000000 parts by mass, more preferably 10 to 100000 parts by mass, and still more preferably from the viewpoint of accurately reproducing the human head odor. It is 30-10000 mass parts, More preferably, it is 50-1000 mass parts.

  As described above, the smell generated by the indicator for body odor determination of the present invention accurately reproduces human body odor, particularly head odor and odor, which is useful for evaluating the deodorizing effect of body odor. is there. Therefore, the deodorizing effect of body odor by the test sample can be evaluated using the indicator for body odor determination of the present invention.

  The method for evaluating the deodorizing effect of body odor according to the present invention is the following: after contacting a test sample and the indicator for body odor determination, the presence or absence of the odor generated by the indicator for body odor determination, the intensity of the odor and the unpleasant odor At least one selected from the group consisting of degrees, from the group consisting of the difference in odor intensity due to the presence or absence of odor, the presence or absence of contact with the test sample and the difference in odor discomfort due to the presence or absence of contact with the test sample The deodorizing effect of body odor is evaluated by at least one selected.

  The method for evaluating the deodorizing effect of body odor according to the present invention can accurately reproduce human body odor by using the body odor determination index agent without using body odor emitted by the subject himself / herself. The odor effect can be objectively and accurately evaluated with a simple operation.

  Examples of the test sample include odor components such as fragrances and flavors, substances expected to absorb or adsorb the indicator for body odor determination, substances expected to change the structure of the indicator for body odor determination, Substance that is expected to decompose the indicator for body odor determination, substance that is expected to suppress volatilization of the indicator for body odor determination, and the indicator for body odor determination from the site where the indicator for body odor determination is attached Although the substance or base material etc. which are anticipated to separate are mentioned, this invention is not limited only to this illustration.

  The contact between the test sample and the indicator for body odor determination can be performed by a method according to the type of the test sample.

  The test sample decomposes the odor component, the substance expected to absorb or adsorb the body odor indicator, the substance expected to change the structure of the body odor indicator, and the body odor indicator Is a substance that is expected to suppress volatilization of the indicator for body odor determination or the body odor determination indicator, the test sample and the indicator for body odor determination are mixed by mixing the test sample with the body odor determination index agent. It can be done by doing.

  When the test sample and the body odor determination index agent are mixed, the mixing ratio of the test sample and the body odor determination index agent [test sample / body odor determination index agent (mass ratio)] is the type of body odor determination index agent, the test Since it differs depending on the type of sample and the evaluation method of the deodorizing effect, it cannot be determined unconditionally. Usually, the mixing ratio of the test sample to the body odor determination index [test sample / body odor determination index (mass ratio)] is from the viewpoint of objectively and accurately evaluating the human body odor deodorizing effect of the test sample. The ratio is preferably 0.1 / 1 to 1000000/1, more preferably 1/1 to 100000/1, and still more preferably 1/1 to 10000/1.

  When the test sample is a substance expected to separate the body odor determination index agent from the site to which the body odor determination index agent is attached, contact between the test sample and the body odor determination index agent depends on the test sample. This can be done by washing the part.

  Examples of the site to which the body odor determination index agent is attached include, for example, the skin of the part where the body odor is generated in the body, the clothes or the fabric product where the generated body odor may be attached, etc. However, the present invention is not limited to such examples.

  The amount of the test sample used for washing varies depending on the site to which the indicator for body odor determination is attached, the type of test sample, etc., and therefore cannot be determined unconditionally. It is desirable to set.

  Furthermore, when the test sample is a base material expected to separate the body odor determination index agent from the site to which the body odor determination index agent is attached, the contact between the test sample and the body odor determination index agent is: This can be done by wiping the site with a test sample.

  Since the size of the base material used for wiping varies depending on the part to which the body odor determination index agent is attached, etc., it cannot be determined unconditionally, such as the part to which the body odor determination index agent is attached, etc. It is preferable to set appropriately according to the above. In addition, the base material expected to separate the body odor determination index agent from the part to which the body odor determination index material is attached includes a base material impregnated with at least one of the substances. In this case, since the amount of the substance contained in the base material varies depending on the site to which the indicator for body odor determination is attached, the type of the test sample, etc., it cannot be determined unconditionally. It is preferable to set appropriately according to the above.

  The presence or absence of the odor generated by the indicator for body odor determination, the intensity of the odor, and the unpleasantness of the odor can be examined, for example, by sensory evaluation.

In the evaluation method of the deodorizing effect of body odor of the present invention,
(A) Presence or absence of smell generated by the indicator for body odor determination,
(B) change in odor intensity before and after contact between the test sample and the body odor determination indicator;
(C) The intensity of the odor emitted by the indicator for body odor determination after contact between the reference substance and the indicator for body odor determination, and the indicator for body odor determination after contact between the test sample and the indicator for body odor determination The difference between the intensity of the smell,
(D) change in odor discomfort before and after contact between the test sample and the body odor determination indicator;
(E) The discomfort of the odor generated by the indicator for body odor determination after contact with the reference substance and the indicator for body odor determination, and the indicator for body odor determination after contact between the test sample and the indicator for body odor determination The deodorizing effect of the body odor by the test sample can be evaluated based on the difference between the discomfort level of the odor generated and the like.

  Here, when the odor emitted by the indicator for body odor determination is eliminated by the test sample, the intensity of the odor emitted by the indicator for body odor determination is reduced by the test sample, or the body odor determination index by the test sample When the discomfort level of the odor generated by the agent is reduced, it can be evaluated that the test sample exhibits a deodorizing effect on body odor. In addition, the degree of change in odor intensity before and after contact between the test sample and the body odor determination index agent, or the intensity of the odor emitted by the body odor determination index agent after contact between the reference substance and the body odor determination index agent, The degree of body odor deodorizing effect by the test sample can be evaluated based on the degree of difference between the test sample and the body odor determination index agent after the contact with the body odor determination index agent.

  The odor emitted by the body odor determination indicator of the present invention accurately reproduces human body odor, particularly head odor and odor, and the detection of the body odor determination indicator is easy. The body odor determination indicator is useful for evaluating the performance of a deodorant agent. Therefore, a deodorant agent can be evaluated using the indicator for body odor determination of the present invention.

  The evaluation method of the deodorant agent of the present invention is to collect a substance present at an application site of the deodorant agent, examine the amount of the indicator agent for body odor determination contained in the substance, and deodorant according to the amount of the indicator agent for body odor determination It is characterized by evaluating the performance of the agent.

  The evaluation method of the deodorant agent of the present invention is not to evaluate the performance of the deodorant agent by evaluating the human body odor after the deodorant agent is attached by sensory evaluation, but the amount of the body odor determination indicator is determined by the amount of the deodorant agent. Since the performance of the deodorant agent is evaluated as an index of performance, the deodorant agent can be objectively and accurately evaluated with a simple operation.

  In the present specification, the “deodorant agent” is a concept including both a deodorant agent applied directly to the skin and a deodorant agent applied to a place where body odor may adhere. Examples of places where the body odor may adhere include hair, clothing, shoes, bedding, carpets, curtains, and the like, but the present invention is not limited to such examples.

When the deodorant agent is applied, the amount of the deodorant agent used varies depending on the type of the deodorant agent, the amount of the active ingredient contained in the deodorant agent, and the like, and thus cannot be determined unconditionally. Usually, the use amount of the deodorant agent is preferably 0.1 to 100 mg, more preferably 1 to 100 cm / cm ² from the viewpoint of evaluating the performance of the deodorant agent reflecting the actual use state of the deodorant agent. 50 mg.

  Examples of locations where the deodorant agent is applied include skin parts such as axilla, trunk, soles, and head, and places where the body odor may adhere, but the present invention is limited only to such examples. Is not to be done.

  Since the contact time of the deodorant agent to the application site varies depending on the type of application site, the type of deodorant agent, the amount of deodorant agent used, the amount of active ingredients contained in the deodorant agent, etc., it cannot be determined unconditionally. . For example, when the application site is a part of the skin, usually, the contact time of the deodorant agent to the skin is preferably 1 to 72 hours, more preferably from the viewpoint that the effect of the deodorant agent can be objectively and accurately determined. 3 to 24 hours.

Collection of the substance present at the application site of the deodorant agent is, for example,
(A) collecting the headspace gas at the application site of the deodorant;
(B) bringing a test piece into contact with the application site of the deodorant agent, and extracting a substance attached to the test piece;
(C) It can be carried out by adding a solvent directly to the application site of the deodorant agent and extracting substances present at the application site.

  Examples of the method for measuring the amount of the indicator for body odor determination contained in the substance present at the application site of the deodorant agent include gas chromatography-mass spectrometry, gas chromatograph-hydrogen flame ion detection method, gas chromatograph-electron. Examples include capture detection methods, high performance liquid chromatograph-ultraviolet visible spectroscopy, high performance liquid chromatograph-mass spectrometry, and detection methods using semiconductor sensors, but the present invention is not limited to such examples. Absent. For example, in the gas chromatograph-mass spectrometry method, the amount of the body odor determination indicator can be calculated from the area of the peak corresponding to the body odor determination indicator in the chromatogram.

  In the deodorant evaluation method of the present invention, the amount of the indicator for body odor determination contained in the substance collected from the skin before application of the deodorant and the body odor judgment contained in the substance present at the application site of the deodorant The performance of the deodorant agent can be evaluated by the difference between the amount of the indicator agent for use. For example, when the amount of the indicator for body odor determination is reduced by the deodorant agent, it can be determined that the deodorant agent has a body odor deodorizing effect and / or a deodorizing effect.

  The odor emitted by the indicator for body odor determination of the present invention accurately reproduces human body odor, particularly head odor and foul odor, and is easy to detect the indicator for body odor determination. The indicator for body odor determination is useful for determining body odor. Therefore, the body odor type or body odor intensity can be determined using the body odor determination indicator of the present invention.

  The body odor determination method of the present invention is to collect a substance present on the skin, examine the amount of the body odor determination indicator contained in the obtained substance, and depending on the amount of the body odor determination indicator, the type of body odor Alternatively, the intensity of body odor is determined.

  The body odor determination method of the present invention does not perform body odor determination by sensory evaluation, but performs body odor determination using the amount of the body odor determination indicator as a body odor index. The subject's body odor type or body odor intensity can be determined accurately and accurately.

The collection of the substance is, for example,
(A) collecting headspace gas at a site on the body surface of the subject;
(B) bringing a test piece into contact with the skin of the subject and extracting a substance attached to the test piece;
(C) It can be carried out by bringing a solvent into contact with the skin of the subject and extracting a substance present on the skin with the solvent.

  Examples of the body surface part include the head, axilla, foot, trunk, and back, but the present invention is not limited to such examples. Examples of the test piece include a cotton sheet, a cellulose sheet, a polyethylene sheet, a polyethylene terephthalate sheet, and the like, but the present invention is not limited to such examples.

  Examples of the method for measuring the amount of the body odor determination indicator contained in the substance present on the skin include, for example, gas chromatograph-mass spectrometry, gas chromatograph-hydrogen flame ion detection method, gas chromatograph-electron capture detection method , High-performance liquid chromatograph-ultraviolet visible spectroscopy, high-performance liquid chromatograph-mass spectrometry, detection method using a semiconductor sensor, and the like, but the present invention is not limited to such examples. For example, in the gas chromatograph-mass spectrometry method, the amount of the body odor determination indicator can be calculated from the area of the peak corresponding to the body odor determination indicator in the chromatogram.

  In the body odor determination method of the present invention, for example, when the substance present on the skin is a substance derived from the head of the subject, the body odor determination index contained in the substance present in the head space of the head When the amount of the agent is 2 ppb or more as the amount of diacetyl, it can be determined that the type of head odor of the subject is the oil odor. In particular, when the amount of the body odor determination indicator contained in the substance present in the head space of the head is 3 ppb or more as the amount of diacetyl, the strength of the subject's head odor, particularly the oil odor It can be determined that the intensity of is high.

  On the other hand, in the body odor determination method of the present invention, for example, when the substance present on the skin is a substance derived from the axilla of the subject, the body odor contained in the substance present in the cotton sheet collected from the axilla When the amount of the indicator for determination is 50 ng or more as the amount of diacetyl, it can be determined that the type of odor of the subject is an acid odor. In particular, when the amount of the indicator for body odor determination contained in the substance present in the cotton sheet collected from the axilla is 100 ng or more as the amount of diacetyl, the strength of the test odor, particularly the strength of acid odor Can be determined to be high.

  It has been found by the present inventors that the diketone compound is produced by a microorganism. Therefore, a substance that acts on a microorganism capable of producing the diketone compound to suppress the production of the diketone compound is useful as a body odor suppressor for suppressing the generation of body odor caused by the body odor determination indicator.

The screening method for body odor suppressor of the present invention is a screening method for body odor suppressor for suppressing the generation of body odor due to the indicator for body odor determination,
(A) culturing a microorganism capable of producing the diketone compound in a medium containing a test substance and a precursor of the diketone compound;
(B) By measuring the number of viable microorganisms and / or the amount of the diketone compound in the culture obtained in the step (A), it is determined whether the test substance suppresses the production of the diketone compound. And (C) the step (B) includes a step of selecting a test substance that suppresses production of a diketone compound as a body odor suppressor.

  In the method for screening body odor suppressors of the present invention, a test substance that suppresses the production of diketone compounds is selected as a body odor suppressor based on the number of viable microorganisms and / or the amount of diketone compounds produced by the microorganisms. Therefore, the body odor inhibitor screening method of the present invention can evaluate the body odor inhibitor objectively and accurately with a simple operation, compared with the case where the body odor inhibitor is selected by sensory evaluation.

  In step (A), the microorganism is cultured in a medium containing a test substance and a precursor of the diketone compound (hereinafter referred to as “medium A”).

  The microorganism has an ability to produce a diketone compound. The microorganism is not limited as long as it has the ability to produce a diketone compound, and examples include soil microorganisms, skin resident microorganisms, intestinal resident microorganisms, microorganisms used in fermented foods, etc. It is not limited only to such illustration. Among the microorganisms, skin-resident microorganisms are preferable from the viewpoint of screening a body odor inhibitor that is more suitable for suppressing human body odor.

  Examples of the skin resident microorganism include Staphylococcus aureus and Staphylococcus epidermidis, but the present invention is not limited to such examples. In the screening method for body odor inhibitor of the present invention, the skin resident microorganism is at least one selected from the group consisting of Staphylococcus aureus and Staphylococcus epidermidis from the viewpoint of excellent production ability of the diketone compound. It is preferable that As Staphylococcus aureus, for example, Staphylococcus aureus NBRC13276 can be used. Further, Staphylococcus epidermidis IAM1296 or the like can be used as Staphylococcus epidermidis. Staphylococcus aureus and Staphylococcus epidermidis are genetically related species and may be species having the ability to produce diketone compounds.

  Examples of the test substance include odor components such as fragrances and flavors, substances expected to absorb or adsorb the indicator for body odor determination, substances expected to change the structure of the indicator for body odor determination, Examples include substances expected to decompose the body odor determination indicator, substances expected to suppress volatilization of the body odor determination index, and the present invention is not limited to such examples. Absent.

  Since the content rate of the test substance in the medium A varies depending on the type of test substance, the use of the test substance, etc., it cannot be determined unconditionally. Usually, the content of the test substance in the medium A is preferably 0.000001 to 5% by mass, more preferably from the viewpoint of selecting a test substance suitable for use as a body odor suppressant for blending with a deodorant agent or the like. Is 0.00001-2 mass%.

  Examples of the precursor of the diketone compound include pyruvic acid, lactic acid, serine, and the like, but the present invention is not limited to such examples. Among these, pyruvic acid and lactic acid are preferable because they are easily assimilated by the microorganism and the production amount of the diketone compound is high, and lactic acid is more preferable.

  The content of the precursor of the diketone compound in the medium A varies depending on the type of the precursor of the diketone compound and cannot be determined unconditionally. Usually, the content of the precursor of the diketone compound is preferably 0.01 to 20 mM, and more preferably 0.1 to 5 mM, from the viewpoint of reproducing conditions when an actual body odor is generated.

  In addition, components other than the test substance and the diketone compound in the medium A may be medium components used for culturing the microorganism. Examples of such medium components include yeast extract, phosphoric acid, magnesium sulfate, sodium chloride, ammonium chloride, manganese chloride, iron chloride, calcium chloride, and the like, but the present invention is not limited to such examples. Absent.

  The culture time of the microorganism varies depending on the type of microorganism, the type of medium, and the like, and thus cannot be determined unconditionally. Usually, the culture time of the microorganism is preferably 0.5 to 72 hours, more preferably 3 to 24 hours, from the viewpoint of sufficiently securing the time required for the production of the diketone compound from the diketone compound precursor by the microorganism. It is.

  Since the culture temperature of the microorganism varies depending on the type of microorganism, it cannot be determined unconditionally. Usually, the culture temperature of the microorganism is preferably 20 to 50 ° C., more preferably 30 to 40 ° C., from the viewpoint of favorably growing the microorganism.

  In step (B), the test substance is measured by measuring the number of viable bacteria and / or the amount of the diketone compound in the culture obtained in step (A) (hereinafter referred to as “culture A”). Determine whether to suppress the formation of diketone compounds.

  The method for measuring the number of viable microorganisms in the culture A is not particularly limited. In the present invention, for example, a colony for applying the culture A to an agar medium and measuring the number of colonies generated after culturing for a certain period of time. Examples thereof include a counting method and a method of measuring the number of the microorganisms in the culture A by microscopic observation.

  As a method for measuring the amount of the diketone compound in the culture A, in the present invention, for example, gas chromatograph-mass spectrometry, gas chromatograph-hydrogen flame ion detection method, gas chromatograph-electron capture detection method, high performance liquid chromatography Examples include graph-ultraviolet visible spectroscopy, high-performance liquid chromatograph-mass spectrometry, and a detection method using a semiconductor sensor, but the present invention is not limited to such examples. For example, in the gas chromatograph-mass spectrometry method, the amount of the diketone compound can be calculated from the area of the peak corresponding to the diketone compound in the chromatogram.

The determination is, for example,
(A) In the step (A), the same operation as in the step (A) is performed except that the medium containing the precursor of the diketone compound is used instead of the medium A. Comparing the viable cell count of the microorganism in the obtained culture (hereinafter referred to as “culture B”) with the viable cell count of the microorganism in the culture A,
(B) comparing the amount of the diketone compound contained in the culture B with the amount of the diketone compound contained in the culture A;
(C) It can be performed by performing the operations (a) and (b). in this case,
(1) The viable count of the microorganism in the culture A is less than the viable count of the microorganism in the culture B, and / or (2) the amount of the diketone compound contained in the culture A is the culture. When satisfy | filling that it is less than the quantity of the said diketone compound contained in B, it can determine with a test substance being a substance which suppresses the production | generation of a diketone compound.

  In step (C), the test substance that suppresses the formation of the diketone compound in step (B) is selected as a body odor suppressor.

  Thus, according to the screening method for body odor suppressor of the present invention, it is possible to screen a body odor suppressor for suppressing the generation of body odor caused by the indicator for body odor determination.

  As described above, according to the body odor determination indicator of the present invention, human body odor can be accurately reproduced. According to the method for evaluating the deodorizing effect of body odor of the present invention, the deodorizing effect of body odor can be objectively and accurately evaluated by a simple operation. Furthermore, according to the method for evaluating a deodorant agent of the present invention, the performance of the deodorant agent can be objectively and accurately evaluated by a simple operation. Moreover, according to the determination method of body odor of this invention, the kind and intensity | strength of body odor can be determined objectively and accurately by simple operation. Moreover, the body odor inhibitor screening method of the present invention can efficiently screen a body odor inhibitor by a simple operation.

  Therefore, the indicator for body odor determination of the present invention, the method for evaluating the deodorizing effect of body odor, the method for evaluating the deodorant agent, the method for determining body odor, and the method for screening body odor inhibitor are cosmetics suitable for humans who care about body odor. It is useful for the development of fragrances and cleaning agents.

  Next, the present invention will be described in more detail based on examples. However, the present invention is not limited to the examples.

(Experimental example 1)
The scalp and hair of each of the 26 subjects (10 healthy Japanese men in their 40s and 50s and 16 healthy Japanese men in their 20s) were washed with unscented shampoo. Four panelists (three men and one woman) evaluated the types of odors in the frontal, top, back, and temporal regions after 12 hours from the end of washing. The evaluation criteria for odor types are as follows.

[Evaluation criteria for odor types]
Sweaty odor: Sour smell when sweating Abra odor: Similar to the smell of old oil, smelled like fermented

  FIG. 1 (A) shows the relationship between the head region and the ratio of sweat odor to head odor in Experimental Example 1, and the ratio of oil odor to head region and head odor in Experimental Example 1 The result of investigating the relationship with is shown in FIG.

  From the results shown in FIG. 1 (A), the ratio of sweat odor to head odor is almost the same between Japanese men in their 20s and Japanese men in their 40s and 50s. Recognize. On the other hand, from the results shown in FIG. 1 (B), the ratio of oil odor to the head odor of Japanese men in their 40s and 50s is the oil odor in the head odor of Japanese men in their 20s. It can be seen that it is higher than the ratio of. Therefore, these results suggest that the oil odor is an odor often found in the scalp of Japanese men in their 40s and 50s.

Example 1
A pillow cover used by a subject having an oily odor emits an oily odor similar to the scalp of the subject. Therefore, a subject having an oil odor was allowed to use a pillow with a non-brominated cotton sheet as a pillow cover for 7 days at bedtime. Next, the cotton sheet was collected and placed in a headspace analysis vial (manufactured by Spellco). Thereafter, a micro solid phase extraction (SPME) fiber (manufactured by Spelco) was inserted into the headspace analysis vial and exposed to the headspace gas phase portion of the cotton sheet for 24 hours.

  Next, the SPME fiber was subjected to a gas chromatograph-mass spectrometer, and components adhering to the cotton sheet brought into contact with the axilla of the subject were analyzed. First, the volatile component adhering to the pillow cover was adsorbed on a micro solid-phase extraction fiber (manufactured by Spelco). The volatile component adsorbed on the fiber was subjected to a gas chromatograph-mass spectrometer (manufactured by Agilent Technology, trade name: 6890GC-5993MSD), and the components contained in the sample were analyzed. The analysis conditions used are as follows.

〔Analysis conditions〕
Column used: Agilent Technology, product name: DB-1701 (60 m × 0.25 mm × 1 μm)
Gas used: helium gas Temperature conditions: 40 ° C. (maintained for 4 minutes), temperature increase from 40 ° C. to 160 ° C. (temperature increase rate 3 ° C./min), maintained at 160 ° C. for 5 minutes and temperature increase from 160 ° C. to 260 ° C. Temperature (temperature increase rate 10 ° C / min)
Ionization method: Electron ionization method (EI), 60 eV

  In Example 1, the result of having analyzed the component adhering to the pillow cover used by the test subject who has an oil odor is shown in FIG. In the figure, the arrow indicates a peak corresponding to diacetyl.

  From the results shown in FIG. 2, it can be seen that a peak is observed at a retention time of about 16 minutes. This peak is presumed to be a peak attributed to diacetyl from the retention time. Therefore, it is suggested that diacetyl adheres to the pillow cover used by the subject having the oil odor.

  Next, the same operation as described above was performed except that a test subject group consisting of seven subjects with abra odor and a non-smeller subject group consisting of eight subjects who did not perceive the scent of abra odor were used as subjects. The components adhering to the pillow covers used by the subjects were analyzed with a gas chromatograph-mass spectrometer. Next, the area of the peak corresponding to diacetyl was calculated using the obtained chromatogram.

  In Example 1, the result of investigating the relationship between the presence or absence of oil odor and the area of the peak corresponding to diacetyl is shown in FIG.

  From the results shown in FIG. 3, the area of the peak corresponding to diacetyl in the component attached to the pillow cover used by the test subject group having the oil odor (see “Abra odor” in the figure) shows that the oil odor is less. It can be seen that it is significantly larger than the area of the peak corresponding to diacetyl in the component attached to the pillow cover used by the undetected group of subjects (see “non-abra odor” in the figure).

  Further, as the test subject, the same operation as described above was carried out except that a test group of testicular odor consisting of six test subjects with abra odor and a test group of non-smellar odor tester consisting of five subjects who were not perceived to have a bad odor, The components adhering to the pillow cover used by each subject were analyzed with a gas chromatograph-mass spectrometer. Next, using the obtained chromatogram, the area of a peak corresponding to diacetyl or a short-chain or medium-chain fatty acid which is a known body odor component was calculated. Using a calibration curve created with a known amount of diacetyl or short or medium chain fatty acid, the amount of diacetyl or short or medium chain fatty acid is determined from the area of the peak corresponding to diacetyl or short or medium chain fatty acid. Calculated.

  FIG. 4 shows the result of examining the amount of each compound detected by the gas chromatograph-mass spectrometer in Example 1. In FIG. 4, Compound No. 1 is diacetyl, Compound No. 2 is a fatty acid having 2 carbon atoms (acetic acid), Compound No. 3 is a fatty acid having 3 carbon atoms (propionic acid), Compound No. 4 is a fatty acid having 4 carbon atoms (butyric acid), Compound No. 5 is a fatty acid having 5 carbon atoms (valeric acid), Compound No. 6 is a fatty acid having 6 carbon atoms (caproic acid), Compound No. 7 is a fatty acid having 7 carbon atoms (enanthic acid), Compound No. 8 is having 8 carbon atoms Fatty acid (caprylic acid), Compound No. 9 represents a fatty acid having 9 carbon atoms (pelargonic acid), and Compound No. 10 represents a fatty acid having 10 carbon atoms (capric acid).

  From the results shown in FIG. 4, the amount of diacetyl and the fatty acid having 6 to 10 carbon atoms contained in the components attached to the pillow cover used by the group of test subjects for the odor of arabic was determined by It can be seen that the amount of diacetyl and the fatty acid having 6 to 10 carbon atoms contained in the adhering component is significantly larger.

  Therefore, these results suggest that diacetyl and a fatty acid having 6 to 10 carbon atoms are related to the oil odor emitted by the pillow cover used by the oil odor test subject group.

(Example 2)
Sixteen subjects [16 Japanese men in their 40s to 50s] were grouped into a group of subjects with strong oil odor on the scalp and a group of subjects with weak oil odor on the scalp. Each subject's scalp and hair were washed with unscented shampoo.

  After 12 hours from the end of washing, 1 L of headspace gas in the head of the subject was collected using a vacuum-like airtight container (product name: MiniCan manufactured by Entech Instruments Inc.). The obtained headspace gas was diluted 3 times with nitrogen to obtain a sample.

  Gas chromatograph-mass spectrometer (trade name: 6890N gas chromatograph manufactured by Agilent Technology, Inc.) connected with an automatic concentrator (product name: 7100A-Preconcentrator) manufactured by Entech Instruments Inc. 5973N Mass Selective Detector] was used to analyze the components contained in the sample. The analysis conditions used are as follows.

〔Analysis conditions〕
Sample injection volume: 1000 mL
Column used: Agilent Technology, product name: DB-1701 (60 m × 0.25 mm × 1 μm)
Gas used: helium gas Temperature conditions: 40 ° C. (maintained for 4 minutes), temperature increase from 40 ° C. to 160 ° C. (temperature increase rate 3 ° C./min), maintained at 160 ° C. for 5 minutes and temperature increase from 160 ° C. to 260 ° C. Temperature (temperature increase rate 10 ° C / min)
Ionization method: Electron ionization method (EI), 60 eV

  In addition, a gas chromatograph-mass analysis connected with an automatic concentrator (manufactured by Entech Instruments Inc., trade name: 7100A-Preconcentrator) and a sniffing port [trade name: ODP2 sniffing port, manufactured by Gester] Using a device (manufactured by Agilent Technology, trade name: 6890N gas chromatograph and 5973N mass selective detector), the main components causing the head odor were investigated.

  In Example 2, the result of analyzing the components contained in the headspace gas of the subject with strong oil odor in the scalp is shown in FIG. 5 (A). The components contained in the headspace gas of the subject with weak oil odor in the scalp are shown in FIG. The analysis result is shown in FIG. In the figure, the arrow indicates a peak corresponding to diacetyl.

  From the results shown in FIG. 5, the peak corresponding to diacetyl contained in the headspace gas of the subject with strong oil odor in the scalp corresponds to diacetyl contained in the headspace gas of the subject with weak oil odor in the scalp. It can be seen that it is about 3 times higher than the peak. In addition, it was confirmed that diacetyl was contained in the headspace gas of all subjects with strong oil odor in the scalp.

  Furthermore, from the results of the smell sniffing gas chromatograph, it was confirmed that the fraction showing the peak corresponding to the diacetyl had a strong smell very similar to the oil odor.

  Generally, the discrimination threshold of diacetyl by humans is said to be 0.00005 ppm. On the other hand, the discrimination threshold value of each fatty acid having 6 to 10 carbon atoms by human is 10 times or more than the discrimination threshold value of diacetyl by human. Therefore, even if the amount of diacetyl is 1/10 of the amount of fatty acids having 6 to 10 carbon atoms, humans feel the smell of diacetyl stronger than the smell of fatty acids having 6 to 10 carbon atoms. Therefore, the above results suggest that diacetyl is a major component of the oil odor in the scalp.

(Example 3)
Thirteen subjects (13 Japanese men in their 40s to 50s) were allowed to use a pillow with a non-brominated cotton sheet as a pillow cover for 7 days at bedtime. Four panelists (three men and one woman) evaluated the type of head odor adhering to the pillowcase to determine the ratio of the oil odor to the head odor. Next, the level of the oil odor was evaluated based on the ratio of the oil odor to the head odor. The evaluation criteria for the level of oil odor are as follows.

[Evaluation criteria for oil odor level]
0 to 9 points: Ratio of oil odor to head odor 0 to 9%
10 to 19 points: Ratio of oil odor to head odor 10 to 19%
20-29 points: Ratio of oil odor to head odor 20-29%
30-39 points: Ratio of oil odor to head odor 30-39%
40-49 points: Ratio of oil odor to head odor 40-49%
50-59 points: Ratio of oil odor to head odor 50-59%
60-69 points: Ratio of oil odor to head odor 60-69%
70 to 79 points: Ratio of oil odor to head odor 70 to 79%
80-89 points: Ratio of oil odor to head odor 80-89%
90-99 points: Ratio of oil odor to head odor 90-99%
100 points: 100% of the oil odor in the head odor

  In Example 1, the same operation as in Example 1 was performed except that 13 subjects [13 Japanese men in their 40s to 50s] were used as subjects, and each subject used. The component adhering to the pillow cover was analyzed with a gas chromatograph-mass spectrometer, and the peak area corresponding to diacetyl was calculated.

  In Example 3, the result of investigating the relationship between the oil odor level and the peak area corresponding to diacetyl is shown in FIG. In the figure, the level of oil odor indicates the average score of the level of oil odor given by four panelists.

  From the results shown in FIG. 6, the smaller the peak area corresponding to diacetyl contained in the component attached to the pillow cover, the smaller the level of oil odor attached to the pillow cover, and the peak area corresponding to the diacetyl. It can be seen that there is a correlation between the level of the oil odor and the area of the peak corresponding to diacetyl contained in the component attached to the pillow cover as the value of is larger.

Example 4
The scalp and hair of each of 16 subjects (16 healthy Japanese men in their 40s to 50s) were washed with unscented shampoo. Four panelists (three men and one woman) evaluated the intensity of head odor at the top of the head at the end of 12 hours from the end of washing. The intensity of the head odor is as follows.

[Evaluation criteria for head odor intensity]
0 point: not smelling 1 point: faintly smelling 2 points: weakly smelling 3 points: clearly smelling 4 points: smelling slightly strong 5 points: smelling very strongly

  In addition, the four panelists evaluated the type of head odor at the top of the head to determine the ratio of the oil odor to the head odor. Next, the level of the oil odor was evaluated based on the ratio of the oil odor to the head odor. The evaluation criteria for the level of oil odor are as follows.

[Evaluation criteria for oil odor level]
0-9 points: 0-9% of oil odor in head odor
10 to 19 points: Ratio of oil odor to head odor 10 to 19%
20-29 points: Ratio of oil odor to head odor 20-29%
30-39 points: Ratio of oil odor to head odor 30-39%
40-49 points: Ratio of oil odor to head odor 40-49%
50-59 points: Ratio of oil odor to head odor 50-59%
60-69 points: Ratio of oil odor to head odor 60-69%
70 to 79 points: Ratio of oil odor to head odor 70 to 79%
80-89 points: Ratio of oil odor to head odor 80-89%
90-99 points: Ratio of oil odor to head odor 90-99%
100 points: ratio of oil odor to head odor: 100%

  Next, the 16 subjects were grouped into three groups based on the intensity of head odor or the level of oil odor. The classification criteria for the head odor level and the oil odor level are as follows. In addition, the intensity | strength of a head odor shows the average value of the score of the intensity | strength of the head odor attached by four panelists. The level of oil odor indicates the average score of the level of oil odor given by four panelists.

[Classification criteria for head odor level]
Head odor intensity 0 to 2 points: weak head odor intensity Head odor intensity 3 to less than 3.5 points: moderate head odor head odor intensity 3.5 points or more: strong head odor

[Classification criteria for level of oil odor]
Level of oil odor 0 to 24 points: weak oil odor Level of oil odor 25 to 39 points: Moderate oil odor Level of oil odor 40 points or more: Strong oil odor

  In Example 2, the same operation as in Example 2 was performed except that 16 subjects [16 Japanese men in their 40s to 50s] were used as subjects. The components contained in were analyzed. Next, the concentration of diacetyl in the headspace gas of the head was calculated using a calibration curve prepared by analyzing a diacetyl standard gas (0.1 to 10 ppb / nitrogen gas) with a known concentration by the same method.

  In Example 4, the result of investigating the relationship between the head odor level and the concentration of diacetyl in the head space gas of the head is shown in FIG. Moreover, in Example 4, the result of investigating the relationship between the level of oil odor and the concentration of diacetyl in the headspace gas in the head is shown in FIG. In the figure, the arrow indicates a peak corresponding to diacetyl.

  From the results shown in FIG. 7, it can be seen that the higher the concentration of diacetyl in the head space gas of the head, the stronger the head odor, and the lower the concentration of diacetyl in the head space gas of the head, the weaker the head odor. Recognize. Therefore, it can be seen from this result that there is a correlation between the level of head odor and the concentration of diacetyl in the head space gas of the head.

  Further, from the results shown in FIG. 8, the higher the concentration of diacetyl in the head space gas of the head, the higher the level of oil odor in the scalp, and the lower the concentration of diacetyl in the head space gas of the head, It can be seen that the level of oil odor is low. Therefore, it can be seen from this result that there is a correlation between the level of oil odor in the scalp and the concentration of diacetyl in the head space gas of the head.

  From the above results, it is suggested that diacetyl can be used as an indicator for body odor determination.

(Experimental example 2)
The axilla of each of 16 subjects [16 healthy Japanese men in their 30s to 50s] was washed with unscented soap. Four panelists (three men and one woman) evaluated the strength of the stink odor, the type of odor and the strength of the axilla 24 hours after the end of washing. The evaluation criteria for the odor or odor intensity and the odor type evaluation criteria are as follows.

[Evaluation criteria for odor or odor intensity]
0 point: not smelling 1 point: faintly smelling 2 points: weakly smelling 3 points: clearly smelling 4 points: smelling slightly strong 5 points: smelling very strongly

[Evaluation criteria for odor types]
Skin odor: smells like milk. Original odor of the skin Acid odor: Sour sultry smell Spicy odor: Spicy odor like cumin oil

  Further, the ratio of skin odor, acid odor or spicy odor to the odor was determined. Next, the level of skin odor, acid odor or spicy odor was evaluated based on the ratio of skin odor, acid odor or spicy odor to the odor. The evaluation criteria for the level of skin odor, acid odor or spicy odor are as follows.

[Evaluation criteria for oil odor level]
0-9 points: Ratio of skin odor, acid odor or spicy odor to odor odor 0-9%
10 to 19 points: The ratio of skin odor, acid odor or spicy odor to the bad odor 10 to 19%
20-29 points: Ratio of skin odor, acid odor or spicy odor to odor 20-29%
30 to 39 points: Ratio of skin odor, acid odor or spicy odor to the bad odor 30 to 39%
40 to 49 points: Ratio of skin odor, acid odor or spicy odor to odor 40 to 49%
50 to 59 points: Ratio of skin odor, acid odor or spicy odor to odor 50 to 59%
60-69 points: 60-69% ratio of skin odor, acid odor or spicy odor to the bad odor
70 to 79 points: Ratio of skin odor, acid odor or spicy odor to the bad odor 70 to 79%
80 to 89 points: Ratio of skin odor, acid odor or spicy odor to the odor 80 to 89%
90 to 99 points: Ratio of skin odor, acid odor or spicy odor to the odor of 90 to 99%
100 points: Ratio of skin odor, acid odor or spicy odor to the odor 100%

  Table 1 shows the results of evaluating the odor intensity, odor type, and intensity of each subject in Experimental Example 2. In the table, the odor intensity indicates the average value of the odor intensity scores given by four panelists. Moreover, the numerical value of the kind of odor in a table | surface shows the average value of the score of the level of the skin odor, acid odor, or spicy odor given by four panelists.

  From the results shown in Table 1, it can be seen that among subjects having the same odor intensity, there are subjects having a strong acid odor and subjects having a strong spicy odor. Moreover, it turns out that the test subject with strong skin odor has the tendency for the intensity | strength of a bad smell to be comparatively weak.

(Example 5)
Among subjects in Experimental Example 3, three subjects with strong skin odor (subject numbers 1 to 3 in Table 1), three subjects with strong acid odor (subject numbers 5 to 7 in Table 1), and subject 3 with strong spicy odor Persons (subject numbers 14-16 in Table 1) were selected. The axilla of each subject was washed with unscented soap. Thereafter, each subject was allowed to wear a T-shirt with a non-brominated cotton sheet affixed to the axilla, and the cotton sheet was brought into contact with the axilla of the subject. After 24 hours from the start of wearing the T-shirt, the cotton sheet was collected from the T-shirt.

  The collected cotton sheet was placed in a headspace analysis vial (manufactured by Spelco). Next, a solid phase microextraction (SPME) fiber (manufactured by Spelco) was inserted into the headspace analysis vial and exposed to the headspace gas phase portion of the cotton sheet for 24 hours.

  Thereafter, the SPME fiber was subjected to a gas chromatograph-mass spectrometer, and components adhering to the cotton sheet brought into contact with the axilla of the subject were analyzed. The analysis conditions used are as follows.

〔Analysis conditions〕
Column used: Agilent Technology, product name: DB-1701 (60 m × 0.25 mm × 1 μm)
Gas used: Helium gas Temperature conditions: −10 ° C. (maintained for 3 minutes), temperature increase from −10 ° C. to 160 ° C. (temperature increase rate 3 ° C./min), temperature increase from 160 ° C. to 280 ° C. (temperature increase rate) 10 ° C) and maintained at 280 ° C for 3 minutes. Ionization method: electron ionization method (EI), 60 eV

  In addition, using the smell sniffing gas chromatograph, the components causing the odor were investigated.

  As a result, a peak corresponding to diacetyl as well as a peak corresponding to saturated fatty acids such as acetic acid and propionic acid, which are known components constituting body odor, were confirmed as components that cause a bad odor.

  Therefore, the area of the peak corresponding to diacetyl was calculated using the obtained chromatogram. Next, the amount of diacetyl per cotton sheet was calculated from the area of the peak corresponding to diacetyl using a calibration curve created using a known amount of diacetyl. The results are shown in Table 2. In Example 5, the relationship between the type of odor and the area of the peak corresponding to diacetyl was examined, and the relationship between the odor type and the area of the peak corresponding to acetic acid was examined in FIG. The results are shown in FIG. In the figure, “M” is a group (hereinafter referred to as “skin odor group”) consisting of subjects (subject numbers: 14 to 16) having low odor intensity and weak acid odor, and “A” is high in odor intensity. In addition, a group consisting of subjects (subject numbers: 5 to 7) having a strong acid odor (hereinafter referred to as “acid odor group”) and “C” are subjects having high odor intensity and strong spicy odor (subject numbers 1 to 1). 3) (hereinafter referred to as “spicy odor group”).

  From the results shown in Table 2, it can be seen that the amount of diacetyl in each of the acid odor group and the spicy odor group is larger than the amount of diacetyl in the skin odor group. Especially, it turns out that the amount of diacetyl in an acid odor group is remarkably large compared with the amount of diacetyl in each skin odor group and spicy odor group.

  Furthermore, the result shown in FIG. 9B shows that the amount of acetic acid is not significantly different among the skin odor group, the acid odor group, and the spicy odor group. However, from the results shown in FIG. 9 (A), the amount of diacetyl in each of the acid odor group and the spicy odor group is larger than the amount of diacetyl in the skin odor group, as in the results shown in Table 2. I understand that.

  Therefore, these results suggest that diacetyl is associated with a bad odor, particularly an acid odor.

(Example 6)
In Example 5, the same operation as in Example 5 was performed except that a test subject having a strong acid odor and a test subject having a weak acid odor were used as subjects, and adhered to a cotton sheet brought into contact with the axilla of the subject. Ingredients were analyzed. In Example 6, the result of analyzing the components attached to the cotton sheet brought into contact with the axilla of the subject with strong acid odor is shown in FIG. The result of analyzing the components attached to the sheet is shown in FIG.

  From the results shown in FIGS. 10 (A) and 10 (B), the peak corresponding to diacetyl attached to the cotton sheet brought into contact with the axilla of the subject with strong acid odor is in the axilla of the subject with weak acid odor. It can be seen that it is significantly higher than the peak corresponding to diacetyl adhering to the contacted cotton sheet. Therefore, these results suggest that the amount of diacetyl correlates with the intensity of odor, particularly acid odor.

  From the above results, it is suggested that diacetyl can be used as an indicator for body odor determination.

(Examples 7 to 12)
By mixing the (A) component, (B) component, (C) component, (D) component and diluent shown in Table 3 so as to have the composition shown in Table 3, as an indicator for body odor determination A pseudo head odor composition was obtained.

(Comparative Example 1)
By mixing the components (B), (C), (D) and diluent shown in Table 3 so as to have the composition shown in Table 3, the pseudo head odor as an indicator for body odor determination A composition was obtained.

(Test Example 1)
Either a pseudo head odor composition as an indicator for body odor determination obtained in Examples 7 to 12 or a pseudo head odor as an indicator for body odor determination obtained in Comparative Example 1 in a non-brominated vial 0.5 g of composition was added. Thereafter, in a room maintained at 25 ° C. and 50% humidity, three panelists (three men) evaluated the odor of the pseudo head odor composition as an indicator for body odor determination, and against the oil odor on the head The approximate head odor composition as an indicator for body odor determination obtained in Examples 7 to 12 and the approximate head odor composition as an indicator for body odor determination obtained in Comparative Example 1 Judged. The results are shown in Table 3. The criteria for evaluating the odor of the simulated head odor composition as an indicator for body odor determination and the criteria for determining the approximate level for the oil odor in the head are as follows.

[Evaluation criteria for odor of pseudo head odor composition as indicator for body odor determination]
5 points: It can be clearly recognized as an oil odor in the head.
4 points: Can be recognized as an oil odor in the head.
3 points: Can be slightly recognized as an oil odor in the head.
2 points: Slightly difficult to recognize as oil odor on head.
1 point: Cannot be recognized as oil odor in the head.

[Judgment standard of approximate level for head odor in head]
◎ (approximate to the oil odor in the head)
... Average score of odor evaluation is 4 or more ○ (approximate to oil odor in head)
... Average score of odor evaluation is 3 or more and less than 4 △ (not very close to the oil odor in the head)
... Average score of odor evaluation score is 2 or more and less than 3 × (It is not approximate to the oil odor in the head)
... Average score of odor evaluation is 1 or more and less than 2

  From the results shown in Table 3, the simulated head odor composition as an indicator for body odor determination obtained in Examples 7 to 12 containing diacetyl emits an odor similar to the oil odor in the head. I understand. Similar results can be obtained when a diketone compound represented by formula (I) other than diacetyl is used instead of diacetyl. In contrast, the pseudo head odor composition as an indicator for body odor determination obtained in Comparative Example 1 that does not contain diacetyl is found to emit an odor that is not very similar to the oil odor in the head.

  Therefore, the pseudo head odor composition as an indicator for body odor determination containing diacetyl can reproduce the smell similar to the oil odor in the head, and therefore targets humans having the oil odor in the scalp. It is useful for the development of deodorant agents, deodorants for nursing care odors, indoor deodorants, deodorants such as deodorizers in public facilities, cleaning agents for clothing, and residential cleaners.

(Examples 13 to 18)
By blending the (A) component, (B) component, (C) component, (D) component and diluent shown in Table 4 so as to have the composition shown in Table 4, as an indicator for body odor determination A pseudo odor composition was obtained.

(Comparative Example 2)
By blending the components (B), (C), (D) and diluent shown in Table 4 so as to have the composition shown in Table 4, a pseudo odor composition as an indicator for body odor determination Got.

(Test Example 2)
Any of the pseudo odor composition as an indicator for body odor determination obtained in Examples 13 to 18 or a pseudo odor composition as an indicator for body odor determination obtained in Comparative Example 2 was added to a non-brominated vial. 5 g was added. Thereafter, under the environment of 25 ° C. and 50% humidity, three panelists (three men) evaluated the odor of the pseudo odor composition as an indicator for body odor determination, and Examples 13 to 18 for acid odor in the armpit The approximate level of odor of each of the pseudo odor composition obtained as an indicator for body odor determination and the pseudo odor composition obtained as an indicator for body odor determination obtained in Comparative Example 1 was determined. The results are shown in Table 3. The evaluation standard of the odor of the pseudo odor composition as an indicator for body odor determination and the determination standard of the approximate level for the acid odor in the axilla are as follows.

[Evaluation criteria for odor of pseudo-odor composition as indicator for body odor determination]
5 points: Clearly recognized as acid odor in the axilla.
4 points: Can be recognized as an acid odor in the axilla.
3 points: Can be slightly recognized as acid odor in axilla.
2 points: Slightly difficult to recognize as acid odor in axilla.
1 point: The acid odor in the axilla cannot be recognized at all.

[Criteria of approximate level for acid odor in axilla]
◎ (It is very close to the acid odor in the axilla)
... Average score of odor evaluation is 4 or more ○ (approximate acid odor in axilla)
... Average score of odor evaluation is 3 or more and less than 4 △ (not very close to acid odor in axilla)
... Average score of odor evaluation score is 2 or more and less than 3 × (It does not approximate the acid odor in axilla)
... Average score of odor evaluation is 1 or more and less than 2

  From the results shown in Table 4, it can be seen that the pseudo odor composition as an indicator for body odor determination obtained in Examples 13 to 18 containing diacetyl emits an odor similar to the acid odor in the armpit. Similar results can be obtained when a diketone compound represented by formula (I) other than diacetyl is used instead of diacetyl. On the other hand, it can be seen that the pseudo odor composition as an indicator for body odor determination obtained in Comparative Example 1 containing no diacetyl emits an odor that is not very similar to the acid odor in the armpit.

  Therefore, since the pseudo odor composition as an indicator for body odor determination containing diacetyl can reproduce an odor similar to the acid odor in the armpits, it is a deodorant and care odor targeting humans with an acid odor. It is useful for the development of deodorants such as deodorants, indoor deodorants, deodorants in public facilities, detergents for clothes, and detergents for residential use.

(Experimental example 3)
Bacteria belonging to the genus Staphylococcus (Staphylococcus epidermidis IAM1296, Staphylococcus aureus NBRC13276, Staphylococcus aphus NBRC13276), Staphylococcus aphus staphus Staphylococcus haemolyticus ATCC 29970) and Corynebacterium bacteria (Corynebacterium jeikeium ATCC 43734), Corynebacterium xerosis (Corynebacter) um xerosis NBRC12684), Corynebacterium minutsumum ATCC23348 and Corynebacterium stratumum (ATCC6940)] as a carbon source and the concentration of pyruvate which is a precursor of diacetyl as a precursor of diacetyl. Medium added to medium (basic medium composition: yeast extract 0.0002% by mass, potassium dihydrogen phosphate 0.003% by mass, dipotassium hydrogen phosphate 0.0019% by mass, magnesium sulfate heptahydrate 0.0002 Mass%, sodium chloride 0.0014 mass%, ammonium chloride 0.001 mass%, magnesium chloride 0.00001 mass%, ferrous chloride 0.00001 mass% In 0.00001 wt%) in the calcium chloride for 24 hours at 36 ° C., and cultured with shaking at 210min ^-1. The obtained culture was used as an analytical sample for analysis of pyruvic acid. Moreover, the component contained in the culture was derivatized with 2,4-dinitrophenylhydrazine to obtain an analytical sample for analysis of diacetyl. The obtained analytical sample was subjected to high performance liquid chromatography (hereinafter referred to as “HPLC”) to examine whether the concentration of pyruvic acid and diacetyl contained in the culture was included. The analysis conditions for pyruvic acid and the analysis conditions for diacetyl are as follows.

[HPLC measurement conditions for analysis of pyruvic acid]
Detection wavelength: 210 nm
Column used: YMC, trade name: Hydrosphere C18
(250mm × 4.6mm * ID)
Column temperature: 30 ° C
Flow rate: 0.7 mL / min
Injection volume: 20 μL
Moving layer: 20 mM sodium hydrogen phosphate aqueous solution (pH 2.5), 0.12 M sodium chloride
(After adjusting pH of 20 mM sodium hydrogenphosphate aqueous solution to 2.5 with phosphoric acid, sodium chloride was dissolved in the resulting mixture so that its concentration was 0.12 M)

[HPLC measurement conditions for diacetyl analysis]
Detection wavelength: 365 nm
Column used: YMC, trade name: Hydrosphere C18
(250mm × 4.6mm * ID)
Column temperature: 50 ° C
Flow rate: 1 mL / min
Injection volume: 0.1 mL
Moving bed: A solution in which acetonitrile and water are mixed at a ratio of 1: 1.

  In Example 3, the relationship between the concentration of pyruvic acid in the culture and the type of Staphylococcus bacteria was examined. FIG. 11A shows the concentration of diacetyl in the culture and the type of Staphylococcus bacteria. The result of investigating the relationship is shown in FIG. In addition, in Experimental Example 3, the relationship between the concentration of pyruvate in the culture and the type of Corynebacterium is shown in FIG. 12A. The concentration of diacetyl in the culture and the Corynebacterium The result of examining the relationship with the type is shown in FIG. In FIG. 11, 1 is Staphylococcus epidermidis, 2 is Staphylococcus aureus, 3 is Staphylococcus hominis, and 4 is Staphylococcus hemolyticus. Further, in FIG. 12, 1 is Corynebacterium J. Caieum, 2 is Corynebacterium xerosis, 3 is Corynebacterium minutsumum, and 4 is Corynebacterium striatam.

  From the results shown in FIG. 11 and FIG. 12, among the Staphylococcus bacteria and Corynebacterium bacteria used, diacetyl is contained in each culture of Staphylococcus aureus and Staphylococcus epidermidis. I understand that. Therefore, it can be seen that Staphylococcus aureus and Staphylococcus epidermidis assimilate pyruvate to produce diacetyl.

(Experimental example 4)
Staphylococcus aureus NBRC13276 and Staphylococcus epidermidis IAM1296 as a basic medium with pyruvic acid, lactic acid, alanine, glycine or serine (0.0002% by mass supplemented as a carbon source) (Composition of basic medium: yeast extract 0.0002% by mass, potassium dihydrogen phosphate 0.003% by mass, dipotassium hydrogen phosphate 0.0019% by mass, magnesium sulfate heptahydrate 0.0002% by mass, sodium chloride 0.0014 mass%, ammonium chloride 0.001 mass%, magnesium chloride 0.00001 mass%, ferrous chloride 0.00001 mass%, calcium chloride 0.00001 mass%) The cells were cultured at 36 ° C. for 5 hours with shaking at 210 min ⁻¹ . Components contained in the obtained culture were derivatized with 2,4-dinitrophenylhydrazine to obtain an analytical sample. The obtained analytical sample was subjected to HPLC, and the concentration of diacetyl in the culture was examined. The concentration of yeast extract, pyruvic acid, lactic acid, alanine, glycine or serine in the medium was 2 mM. The HPLC measurement conditions are as follows.

[HPLC measurement conditions]
Detection wavelength: 365 nm
Column used: YMC, trade name: Hydrosphere C18
(250mm × 4.6mm * ID)
Column temperature: 50 ° C
Flow rate: 1 mL / min
Injection volume: 0.1 mL
Moving bed: A solution in which acetonitrile and water are mixed at a ratio of 1: 1.

  FIG. 13 shows the results of examining the relationship between the type of medium and the concentration of diacetyl in the culture in Experimental Example 4. In the figure, 1 is a basic medium, 2 is a medium supplemented with pyruvic acid, 3 is a medium supplemented with lactic acid, 4 is a medium supplemented with alanine, 5 is a medium supplemented with glycine, and 6 is supplemented with serine. The broken medium is shown.

  From the results shown in FIG. 13, in the case of culturing both Staphylococcus aureus and Staphylococcus epidermidis, when using pyruvic acid or lactic acid as a carbon source, compared to using other carbon sources It can be seen that the concentration of diacetyl in the culture becomes high. Therefore, these results indicate that pyruvic acid or lactic acid is used as a precursor of diacetyl in Staphylococcus aureus and Staphylococcus epidermidis.

(Example 19)
A medium in which a test substance and lactic acid are added to a basic medium so that the respective concentrations are 0.01% by mass and 2 mM (basic medium composition: yeast extract 0.0002% by mass, potassium dihydrogen phosphate 0.003% by mass , Dipotassium hydrogen phosphate 0.0019 mass%, magnesium sulfate heptahydrate 0.0002 mass%, sodium chloride 0.0014 mass%, ammonium chloride 0.001 mass%, magnesium chloride 0.00001 mass%, chloride chloride The concentration of Staphylococcus aureus NBRC13276 is 1 × 10 ⁷ CFU / mL in ferrous iron (0.00001 mass%, calcium chloride 0.00001 mass%) (hereinafter referred to as “medium A”). And cultured at 36 ° C. for 6 hours while shaking at 210 min ^−1. It was. As a test substance, triclosan, 4-isopropyl-3-methylphenol, tocopherol, ascorbyl isopalmitate or pantothenyl alcohol was used. Triclosan and 4-isopropyl-3-methylphenol are substances used as a bactericidal component in the deodorant agent. Lactic acid is a substance considered to be a precursor of diacetyl.

  Of the obtained culture, 0.2 mL of the culture was collected. After the components contained in the collected culture were derivatized with 2,4-dinitrophenylhydrazine, the obtained product was filtered through a membrane filter to obtain a filtrate. The obtained filtrate was subjected to HPLC [manufactured by Shimadzu Corporation, trade name: LC10Avp-UV-visible detector], and the amount of diacetyl contained in the filtrate (amount of diacetyl when cultured in the presence of the test substance) Was measured. The HPLC measurement conditions are as follows.

[HPLC measurement conditions]
Detection wavelength: 365 nm
Column used: YMC, trade name: Hydrosphere C18
(250mm × 4.6mm * ID)
Column temperature: 50 ° C
Flow rate: 1 mL / min
Injection volume: 0.1 mL
Moving bed: A solution in which acetonitrile and water are mixed at a ratio of 1: 1.

  Further, instead of the medium A, a medium in which 1,3-butylene glycol (control) and lactic acid were added to the basic medium so that the respective concentrations were 0.01% by mass and 2 mM (basic medium composition: yeast extract). 0.0002 mass%, potassium dihydrogen phosphate 0.003 mass%, dipotassium hydrogen phosphate 0.0019 mass%, magnesium sulfate heptahydrate 0.0002 mass%, sodium chloride 0.0014 mass%, ammonium chloride 0.001% by mass, magnesium chloride 0.00001% by mass, ferrous chloride 0.00001% by mass, calcium chloride 0.00001% by mass). The amount of diacetyl contained (the amount of diacetyl when cultured in the presence of a control substance) was measured.

  Then formula (II):

The inhibition rate was calculated according to In addition, the number of viable bacteria contained in 1 mL of the culture was measured. The results of examining the relationship between the type of test substance and the inhibition rate in Example 19 are shown in FIG. In the figure, 1 represents triclosan, 2 represents 4-isopropyl-3-methylphenol, 3 represents tocopherol, 4 represents ascorbyl isopalmitate, and 5 represents pantothenyl alcohol. In the figure, the white bar indicates a test substance that reduces the viable count of Staphylococcus aureus, and the black bar indicates a test substance that does not reduce the viable count of Staphylococcus aureus.

  From the results shown in FIG. 14, the inhibition rate when triclosan, 4-isopropyl-3-methylphenol, tocopherol or ascorbyl isopalmitate is used as the test substance is 15% or more. The substance is found to inhibit the production of diacetyl. In contrast, the inhibition rate when pantothenyl alcohol was used as the test substance was 0%, indicating that the production of diacetyl from lactic acid by Staphylococcus aureus was not inhibited.

  Therefore, these results suggest that triclosan, 4-isopropyl-3-methylphenol, tocopherol and ascorbyl isopalmitate are substances that can suppress the occurrence of oily odor in the scalp and acid odor in the axilla. The Triclosan and 4-isopropyl-3-methylphenol reduce the viable count of Staphylococcus aureus, so that the amount of diacetyl is reduced by sterilizing Staphylococcus aureus. It is suggested. On the other hand, tocopherol and ascorbyl isopalmitate do not cause a decrease in the number of viable bacteria, indicating that the production system of diacetyl in Staphylococcus aureus is suppressed. In addition, instead of Staphylococcus aureus, staphylococcus epidermidis IAM1296, Staphylococcus aureus and Staphylococcus epidermidis are genetically related species, and can produce diketone compounds. Similar results are obtained when using species with

  Based on the above results, a microorganism capable of producing a diketone compound such as Staphylococcus aureus is cultured in the presence of a test substance and a diketone compound precursor such as diacetyl, and the amount of diacetyl contained in the culture. It is suggested that it can be evaluated whether or not the test substance can suppress the occurrence of the oil odor in the scalp and the acid odor in the axilla.

(Example 20)
An appropriate amount of a test substance and an appropriate amount of an indicator for body odor determination are placed in a headspace analysis vial (manufactured by Spelco) and left for a certain period of time. Thereafter, an SPME fiber (manufactured by Spelco) is inserted into the headspace analysis vial and exposed to the headspace gas phase for 1 hour.

  Next, the SPME fiber is subjected to a gas chromatograph-mass spectrometer, and the amount (A) of the body odor determination indicator is examined.

  Further, the same operation as described above is performed except that a control substance is used in place of the test substance, and the amount (B) of the indicator for body odor determination is examined.

  Thereafter, the amount (A) of the body odor determination index agent and the amount (B) of the body odor determination index agent are compared to determine whether or not the body odor determination index agent is decreased by the test substance. When the indicator for body odor determination is decreased by the test substance, the test substance is evaluated as having a deodorizing effect.

(Example 21)
Wash the subject's axilla with unscented soap. Next, a deodorant agent is applied to the subject's axilla. Thereafter, each subject wears a T-shirt with a non-brominated cotton sheet attached to the axilla, and the cotton sheet is brought into contact with the axilla of the subject. After 24 hours from the start of wearing the T-shirt, the cotton sheet is collected from the T-shirt.

  The collected cotton sheet is put into a headspace analysis vial (manufactured by Spelco). Next, an SPME fiber (manufactured by Spelco) is inserted into the headspace analysis vial and exposed to the headspace gas phase portion of the cotton sheet for 24 hours.

  Thereafter, the SPME fiber is subjected to a gas chromatograph-mass spectrometer, and the amount (C) of the indicator for body odor determination attached to the cotton sheet brought into contact with the armpit of the subject is analyzed.

  Further, except that no deodorant agent is applied to the subject's axilla, the same operation as described above is performed, and the amount (D) of the indicator for body odor determination attached to the cotton sheet brought into contact with the subject's axilla is analyzed. .

  Thereafter, the amount (C) of the body odor determination index agent and the amount (D) of the body odor determination index agent are compared to determine whether or not the body odor determination index agent is decreased by the deodorant agent. When the indicator for body odor determination is decreased by the test substance, the deodorant is evaluated as having a deodorizing effect and / or a deodorizing effect.

(Example 22)
The deodorant is sprayed on the subject's clothes. Thereafter, the clothes are collected after 24 hours from the spraying of the deodorant.

  Part of the collected clothes is placed in a headspace analysis vial (manufactured by Spellco). Next, a solid phase microextraction (SPME) fiber (manufactured by Spelco) is inserted into the headspace analysis vial and exposed to the headspace gas phase for 24 hours.

  Thereafter, the SPME fiber is subjected to a gas chromatograph-mass spectrometer, and the amount (E) of the indicator for body odor determination is analyzed.

  Further, except that the deodorant agent is not sprayed on the clothes of the subject, the same operation as described above is performed to analyze the amount (F) of the body odor determination indicator.

  Thereafter, the amount (E) of the body odor determination index agent and the amount (F) of the body odor determination index agent are compared to determine whether or not the body odor determination index agent is decreased by the deodorant agent. When the indicator for body odor determination is decreased by the test substance, the deodorant is evaluated as having a deodorizing effect and / or a deodorizing effect.

Claims (1)

A screening method for a body odor suppressor for suppressing the generation of body odor caused by an indicator for body odor determination containing diacetyl, wherein the body odor is an odor generated from the surface of the skin,
(A) culturing a microorganism capable of producing diacetyl in a medium containing a test substance and a precursor of diacetyl,
(B) determining whether the test substance suppresses the production of diacetyl by measuring the viable count of the microorganism and / or the amount of diacetyl in the culture obtained in step (A); and (C) in the step (B), the inhibiting test substance the production of diacetyl, seen including the step of selecting a body odor suppressing agent, microorganisms, Staphylococcus Oureusu and Staphylococcus having ability to produce the diacetyl A screening method for a body odor inhibitor, which is at least one selected from the group consisting of epidermidis .