JP5926894B2 - 家禽類用飼料および当該飼料を用いた脂肪肝の製造方法 - Google Patents
家禽類用飼料および当該飼料を用いた脂肪肝の製造方法 Download PDFInfo
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- Life Sciences & Earth Sciences (AREA)
- Birds (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
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- Feed For Specific Animals (AREA)
Description
(1) 粗タンパク質含量が11重量%以下である家禽類用飼料。
(2) 家禽類が、ニワトリ、ガチョウ、カモ、アヒル、ウズラ、七面鳥、ダチョウ、またはキジである、(1)に記載の家禽類用飼料。
(3) 粗タンパク質含量が11重量%以下である家禽類用飼料を家禽類に給与することを特徴とする、家禽類の脂肪肝の製造方法。
(5) 給与する時期が、家禽類が出荷日齢に達する直前の2〜7日間、または、家禽類が出荷日齢に達した直後の2〜7日間である、(3)または(4)に記載の方法。
(6) 家禽類が、ニワトリ、ガチョウ、カモ、アヒル、ウズラ、七面鳥、ダチョウ、またはキジである、(3)〜(5)のいずれかに記載の方法。
(実施例1)
(1) 供試鶏および飼料の給与
1日齢のブロイラーチャンキー種を導入し、市販のブロイラー前期飼料(粗タンパク質(CP)含量22%、代謝エネルギー(ME)3000kcal/kg、鹿島飼料株式会社製)で10日齢まで飼育した。低タンパク質飼料として下記表1に示す組成のCP7.5%飼料および下記表2に示す組成のCP11%飼料、対照飼料として下記表3に示す組成のCP20%飼料をそれぞれ調製した。
各飼料給与区のブロイラーについて、試験終了時に体重を測定し、肝臓、浅胸筋、および腹腔内脂肪を採取した。それぞれの臓器は重量を測定後、-80℃で保存した。肝臓については、写真を撮影し、脂肪肝の度合いを目視により調べた。また、各飼料給与区の飼料摂取量および飼料効率(飼料摂取量/増体量)も求めた。結果を下記表4および図1に示す。
肝臓0.5gを採取し、Folch法を用いて肝臓脂肪抽出液を作成し、重量法により総脂肪含量(トリグリセライド、コレステロール、その他の脂質の総量)を求めた。トリグリセライド含量の測定は、肝臓脂質抽出液0.01mlまたは0.1mlをそれぞれ0.3mlまたは0.5mlの10%(v/v)TritonX-100-イソプロパノールで置換し、トリグリセライドE-テストワコー(和光純薬)を用いた酵素法により行った。コレステロール含量の測定は肝臓脂質抽出液0.05mlまたは0.1mlを0.3mlの10%(v/v)TritonX-100-イソプロパノールで置換し、コレステロールE-テストワコー(和光純薬)を用いた酵素法により行った。肝臓脂肪中のトリグリセライドならびにコレステロール以外の脂肪はその他の脂質として表した。
(1) 供試鶏および飼料の給与
1日齢のブロイラーチャンキー種を導入し、市販のブロイラー前期飼料(粗タンパク質(CP)含量22%、代謝エネルギー(ME)3000kcal/kg、鹿島飼料株式会社製)で14日齢まで飼育した。低タンパク質飼料として前記表1に示す組成のCP7.5%飼料、対照飼料として前記表3に示す組成のCP20%飼料を調製した。
各飼料給与区のブロイラーについて、試験終了時に体重を測定し、肝臓、浅胸筋、および腹腔内脂肪を採取した。それぞれの臓器は重量を測定後、-80℃で保存した。肝臓については、写真を撮影し、脂肪肝の度合いを目視により調べた。試験結果を図3〜6に示す。
肝臓0.5gを採取し、Folch法を用いて肝臓脂肪抽出液を作成し、重量法により総脂肪含量を求めた。トリグリセライド含量測定は、肝臓脂質抽出液0.01mlまたは0.1mlをそれぞれ0.3mlまたは0.5mlの10%(v/v)TritonX-100-イソプロパノールで置換し、トリグリセライドE-テストワコー(和光純薬)を用いた酵素法により行った。
Claims (6)
- 粗タンパク質含量が7〜11重量%である家禽類用飼料。
- 家禽類が、ニワトリ、ガチョウ、カモ、アヒル、ウズラ、七面鳥、ダチョウ、またはキ
ジである、請求項1に記載の家禽類用飼料。 - 粗タンパク質含量が7〜11重量%である家禽類用飼料を家禽類に給与することを特徴とする、家禽類の脂肪肝の製造方法。
- 給与する期間が2〜7日間である、請求項3に記載の方法。
- 給与する時期が、家禽類が出荷日齢に達する直前の2〜7日間、または、家禽類が出荷
日齢に達した直後の2〜7日間である、請求項3または4に記載の方法。 - 家禽類が、ニワトリ、ガチョウ、カモ、アヒル、ウズラ、七面鳥、ダチョウ、またはキ
ジである、請求項3〜5のいずれかに記載の方法。
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