JP5913316B2 - ニューカッスル病ウイルスをベクターとするヘルペスウイルスワクチン - Google Patents
ニューカッスル病ウイルスをベクターとするヘルペスウイルスワクチン Download PDFInfo
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Description
本願は、2010年8月31日出願の米国仮出願出願番号第61/378,575号の利益を主張する。
本発明は、NDVをベクターとするヘルペスウイルスワクチンまたは組成物を含む。
本開示、特にその特許請求の範囲および/またはパラグラフにおいて、用語“含む(comprises)”、“含んだ(comprised)”、“含むこと(comprising)”などは、米国特許法においてそれらに対して付与された意味を有しうることに注意されたい。例えば、それらは“含む(includes)”、“含んだ(included)”、“含むこと(including)”などを意味することができる。また、用語“本質的に〜からなること(consisting essentially of)”および“本質的に〜からなる(consists essentially of)”などは、米国特許法においてそれらに付与された意味を有することにも注意されたい。例えば、それらは、明記されていない要素を許容するが、先行技術中に存在するかまたは本発明の基本的または新規な特徴に影響を及ぼす要素を除外する。
(式中、R1は、12〜18炭素原子を有する飽和または不飽和の直鎖脂肪族基であり、R2は2または3炭素原子を含有する他の脂肪族基であり、Xは、アミンまたはヒドロキシル基(例えばDMRIE)である)を有するカチオン性脂質である。他の実施形態において、カチオン性脂質は、中性脂質、例えばDOPEを会合させることができる。
(式中、R1およびR2は同一または異なることができ、HまたはCH3を表し;
x=0または1であり、好ましくはx=1であり;
y=1または2であり、ここでx+y=2である)。
EMAに関しては、x=0であり、y=2である。カルボマーに関しては、x=y=1である。
NDVゲノムに挿入されるFHV gB遺伝子を哺乳動物での発現のためにコドン最適化した。合成FHV gB遺伝子(配列番号2)をpBR322ベースベクターにクローニングして、図3に示すように、挿入カセットを含むプラスミドpFR13を得た。プラスミドpFR13をPacIおよびFseIで消化し、大きさが3105bpのPacI-FseI断片を作成した。プラスミドpIV029(US2010/0255029)をPacIおよびFseIで消化し、大きさが19140bpのFseI-PacI断片を作成した。2つの断片をライゲーションしてプラスミドpFR14を作成した(図4)。
NDVはマイナス鎖RNAウイルスであり、遺伝子改変NDVウイルスの作成には逆遺伝学系が必要である。完全長ウイルスゲノムRNAの転写ならびにNP、PおよびLタンパク質の同時発現により、RNPのアセンブリおよび、プラス鎖RNAゲノムのマイナス鎖RNAゲノムへの転写が可能になる。これにより、NDVウイルスの通常の複製サイクルが開始され、感染性粒子の生成が可能となる(図2参照)。
FHV gD遺伝子を発現する操作されたNDVベクターを作成するために、以下の試薬および条件を用いた。転写プラスミドとしてプラスミドpFR16(実施例1参照)を用いた。NP、PおよびLタンパク質の発現プラスミドとしてプラスミドpIV32、pIV33およびpIV34(US2010/0255029)を用いた。T7 RNAポリメラーゼプラスミドとしてプラスミドpNS151(US2010/0255029)を用いた。図2Cに概略を示すように、これらの5つのプラスミドをチャイニーズハムスター卵巣(CHO)細胞に同時トランスフェクトした。CHO細胞のトランスフェクションの72時間後、10日齢の孵化鶏卵にCHO上清を接種し、ウイルスを増殖させた。3日後、尿膜液を採取し、ニワトリの赤血球を用いて赤血球凝集活性(HA)をチェックした。NDV-FHV gDの感染性粒子が首尾よく得られた。
FR09:CGCAGCTGCAATCAATTCAG(配列番号25)
FR10:TGGGTGGACAGGGATCTGCT(配列番号26)
を用いた。
9〜11週齢のSPF(特定病原体不在)ネコ32匹を本研究に含めた。要素数4の割付表を用いて、同腹子、性別、年齢に従って、ネコをネコ8匹の4群(群A〜D)に無作為に割り付けた。地域の飼育および動物福祉の手順に従って、ネコをケアし、収容した。
細書に参照により組み込まれた任意の製品に関する任意の製造業者の使用説明書、説明書、製品仕様書および製品シートと共に参照により本願に組み込まれ、本発明の実施に用いることができる。
本発明のまた別の態様は、以下のとおりであってもよい。
〔1〕(i)1以上の組換えニューカッスル病ウイルス(NDV)-ヘルペスウイルスベクターおよび(ii)薬学的または獣医学的に許容される担体を含む組成物またはワクチン。
〔2〕組換えNDV-ヘルペスウイルスベクターが、1以上のヘルペスウイルス抗原、ポリペプチドまたはそれらのバリアントをコードする1以上の異種ポリヌクレオチドを含む、前記〔1〕に記載の組成物またはワクチン。
〔3〕ヘルペスウイルス抗原またはポリペプチドが、ヘルペスウイルス糖タンパク質B(gB)もしくは糖タンパク質D(gD)またはそれらのバリアントである、前記〔2〕に記載の組成物またはワクチン。
〔4〕組換えNDV-ヘルペスウイルスベクターが、配列番号1または配列番号4に対して少なくとも90%の配列同一性を有するポリペプチドをコードするポリヌクレオチドを含む、前記〔2〕または〔3〕に記載の組成物またはワクチン。
〔5〕組換えNDV-ヘルペスウイルスベクターが、配列番号2、3、5または6に対して少なくとも90%の配列同一性を有するポリヌクレオチドを含む、前記〔2〕または〔3〕に記載の組成物またはワクチン。
〔6〕組成物またはワクチンが、1つまたは2つのNDV-ヘルペスウイルスベクターを含む、前記〔1〕〜〔5〕のいずれか1つに記載の組成物またはワクチン。
〔7〕組成物またはワクチンが、ヘルペスウイルスgB抗原またはそれらのバリアントを含む第1NDV-ヘルペスウイルス組換えベクターおよび、ヘルペスウイルスgD抗原またはそれらのバリアントを含む第2NDV-ヘルペスウイルス組換えベクターを含む、前記〔6〕に記載の組成物またはワクチン。
〔8〕組成物またはワクチンが、ヘルペスウイルスgB抗原もしくはそれらのバリアント、ヘルペスウイルスgD抗原もしくはそれらのバリアントまたはそれらの組み合わせを含むNDV-ヘルペスウイルス組換えベクターを含む、前記〔6〕に記載の組成物またはワクチン。
〔9〕NDV-ヘルペスウイルス組換えベクターが、配列番号1または配列番号4に対して少なくとも90%の配列同一性を有するポリペプチドをコードするポリヌクレオチドを含む、前記〔6〕〜〔8〕のいずれか1つに記載の組成物またはワクチン。
〔10〕組換えNDV-ヘルペスウイルスベクターが、配列番号2、3、5または6に対して少なくとも90%の配列同一性を有するポリヌクレオチドを含む、前記〔6〕〜〔8〕のいずれか1つに記載の組成物またはワクチン。
〔11〕ヘルペスウイルスgB抗原もしくはそれらのバリアント、ヘルペスウイルスgD抗原もしくはそれらのバリアントまたはそれらの組み合わせをコードする1以上のポリヌクレオチドを含む組換えNDV-ヘルペスウイルスベクター。
〔12〕1以上のポリヌクレオチドが、配列番号1または配列番号4に対して少なくとも90%の配列同一性を有する1以上のポリペプチドをコードする、前記〔11〕に記載の組換えNDV-ヘルペスウイルスベクター。
〔13〕1以上のポリヌクレオチドが、配列番号2、3、5または6に対して少なくとも90%の配列同一性を有する、前記〔11〕に記載の組換えNDV-ヘルペスウイルスベクター。
〔14〕NDV-ヘルペスウイルスベクターが、配列番号1に対して少なくとも90%の配列同一性を有するポリペプチドをコードするポリヌクレオチドを含む、前記〔11〕〜〔13〕のいずれか1つに記載の組換えNDV-ヘルペスウイルスベクター。
〔15〕NDV-ヘルペスウイルスベクターが、配列番号4に対して少なくとも90%の配列同一性を有するポリペプチドをコードするポリヌクレオチドを含む、前記〔11〕〜〔13〕のいずれか1つに記載の組換えNDV-ヘルペスウイルスベクター。
〔16〕NDV-ヘルペスウイルスベクターが、配列番号1または配列番号4に対して少なくとも90%の配列同一性を有するポリペプチドをコードする2つのポリヌクレオチドを含む、前記〔11〕〜〔13〕のいずれか1つに記載の組換えNDV-ヘルペスウイルスベクター。
〔17〕1以上のポリヌクレオチドがNDV Avinewゲノムの非必須領域に挿入される、前記〔11〕〜〔16〕のいずれか1つに記載の組換えNDV-ヘルペスウイルスベクター。
〔18〕動物においてヘルペスウイルスに対する防御反応を誘導する方法であって、少なくとも1つのヘルペスウイルス抗原を発現する組換えNDV-ヘルペスウイルスベクターおよび薬学的または獣医学的に許容される担体、アジュバント、賦形剤またはビヒクルを前記動物に投与することを含む前記方法。
〔19〕NDV-ヘルペスウイルスベクターが、配列番号1または配列番号4に対して少なくとも90%の配列同一性を有する1以上のポリペプチドをコードする1以上のポリヌクレオチドを含む、前記〔18〕に記載の方法。
〔20〕投与が口鼻内、点眼薬、スプレー、飲料水、卵内、筋肉内投与であるか、あるいは皮下投与、皮内、経皮である、前記〔18〕または〔19〕のいずれか1つに記載の方法。
〔21〕投与がプライム-ブーストである、前記〔18〕〜〔20〕のいずれか1つに記載の方法。
〔22〕動物がネコ科動物またはイヌ科動物である、前記〔18〕〜〔21〕のいずれか1つに記載の方法。
Claims (14)
- 組成物またはワクチンであって、該組成物又はワクチンが、
(i)ネコヘルペスウイルスgB抗原をコードする異種ポリヌクレオチドを含む第1の組換えNDV-ヘルペスウイルスベクター、及び、ネコヘルペスウイルスgD抗原をコードする異種ポリヌクレオチドを含む第2の組換えNDV-ヘルペスウイルスベクター、
又は、
ネコヘルペスウイルスgB抗原をコードする異種ポリヌクレオチド及びネコヘルペスウイルスgD抗原をコードする異種ポリヌクレオチドを含む組換えNDV-ヘルペスウイルスベクターと、
(ii)薬学的または獣医学的に許容される担体とを含み、
該組換えNDV-ヘルペスウイルスベクターが、配列番号27に示されるゲノムを含む、該組成物又はワクチン。 - 組換えNDV-ヘルペスウイルスベクターが、配列番号1または配列番号4に対して少なくとも90%の配列同一性を有するポリペプチドをコードするポリヌクレオチドを含む、請求項1に記載の組成物またはワクチン。
- 組換えNDV-ヘルペスウイルスベクターが、配列番号2、3、5または6に対して少なくとも90%の配列同一性を有するポリヌクレオチドを含む、請求項1に記載の組成物またはワクチン。
- ヘルペスウイルスgB抗原及びヘルペスウイルスgD抗原をコードする1以上のポリヌクレオチドを含む組換えNDV-ヘルペスウイルスベクターであって、該組換えNDV-ヘルペスウイルスベクターが配列番号27に示されるゲノムを含む、該組換えNDV-ヘルペスウイルスベクター。
- 1以上のポリヌクレオチドが、配列番号1または配列番号4に対して少なくとも90%の配列同一性を有する1以上のポリペプチドをコードする、請求項4に記載の組換えNDV-ヘルペスウイルスベクター。
- 1以上のポリヌクレオチドが、配列番号2、3、5または6に対して少なくとも90%の配列同一性を有する、請求項4に記載の組換えNDV-ヘルペスウイルスベクター。
- NDV-ヘルペスウイルスベクターが、配列番号1に対して少なくとも90%の配列同一性を有するポリペプチドをコードするポリヌクレオチドを含む、請求項4〜6のいずれか1つに記載の組換えNDV-ヘルペスウイルスベクター。
- NDV-ヘルペスウイルスベクターが、配列番号4に対して少なくとも90%の配列同一性を有するポリペプチドをコードするポリヌクレオチドを含む、請求項4〜6のいずれか1つに記載の組換えNDV-ヘルペスウイルスベクター。
- 1以上のポリヌクレオチドが、配列番号27に示されるNDVゲノムの非必須領域に挿入されている、請求項4〜8のいずれか1つに記載の組換えNDV-ヘルペスウイルスベクター。
- 非ヒト動物においてヘルペスウイルスに対する防御反応を誘導する方法であって、請求項4に記載の組換えNDV-ヘルペスウイルスベクターおよび薬学的または獣医学的に許容される担体、アジュバント、賦形剤若しくはビヒクル、又は、請求項1に記載の組成物若しくはワクチンを前記非ヒト動物に投与することを含む前記方法。
- NDV-ヘルペスウイルスベクターが、配列番号1または配列番号4に対して少なくとも90%の配列同一性を有する1以上のポリペプチドをコードする1以上のポリヌクレオチドを含む、請求項10に記載の方法。
- 投与が口鼻内、点眼薬、スプレー、飲料水、卵内、筋肉内投与であるか、あるいは皮下投与、皮内、経皮である、請求項10または11のいずれか1つに記載の方法。
- 投与がプライム-ブーストである、請求項10〜12のいずれか1つに記載の方法。
- 動物がネコ科動物またはイヌ科動物である、請求項10〜13のいずれか1つに記載の方法。
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US8986706B2 (en) | 2015-03-24 |
US9446118B2 (en) | 2016-09-20 |
EP3156070A3 (en) | 2017-06-14 |
JP2013536847A (ja) | 2013-09-26 |
EP2611460A1 (en) | 2013-07-10 |
CA2809127C (en) | 2019-04-02 |
CN103269714B (zh) | 2016-06-29 |
US20150157704A1 (en) | 2015-06-11 |
EP3156070A2 (en) | 2017-04-19 |
ES2609847T3 (es) | 2017-04-24 |
BR112013007486B1 (pt) | 2020-01-28 |
WO2012030720A1 (en) | 2012-03-08 |
EP2611460B1 (en) | 2016-10-05 |
BR112013007486A2 (pt) | 2018-05-02 |
MX344103B (es) | 2016-12-05 |
CA2809127A1 (en) | 2012-03-08 |
EP4014989A1 (en) | 2022-06-22 |
US20120052089A1 (en) | 2012-03-01 |
MX2013002181A (es) | 2014-01-24 |
CN103269714A (zh) | 2013-08-28 |
PL2611460T3 (pl) | 2017-02-28 |
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