JP5868803B2 - Bactericidal solution and bactericidal solution manufacturing apparatus - Google Patents

Description

  The present invention relates to a sterilizing solution for sterilizing an object and a sterilizing solution manufacturing apparatus.

  Conventionally, in order to sterilize an object such as a medical instrument, irradiation with radiation or an electron beam (hereinafter simply referred to as radiation or the like) is performed. However, radiation or the like may adversely affect the human body. Therefore, it is necessary to enclose the periphery of the irradiated portion with a thick wall such as concrete so that the irradiated radiation or the like does not leak to the outside and affect the human body, and the apparatus is large. Moreover, the irradiation apparatus which irradiates radiation etc. is expensive.

  Then, the technique which disinfects a target object by making a target object contact the water (ozone aqueous solution) which ozone melt | dissolved is disclosed (for example, patent document 1).

JP 2008-113682 A

  In the sterilization using the above-described ozone aqueous solution, ozone is generated using oxygen in the atmosphere, so that the raw material cost is hardly required and the object can be sterilized at a low cost. However, there is room for further improving the sterilization efficiency in the sterilization using ozone.

  Then, in view of such a subject, this invention aims at providing the sterilization solution which can further improve sterilization efficiency in the sterilization using ozone aqueous solution, and a sterilization solution manufacturing apparatus.

In order to solve the above problems, sterile solutions of the present invention is a disinfecting solution to sterilize the object, and ozone, chloro and perbenzoic acid, and water, and, in either or both of the water-soluble solvent It is a dissolved solution.

In order to solve the above-mentioned problem, the sterilizing solution of the present invention is a sterilizing solution for sterilizing an object, wherein ozone and m-chloroperbenzoic acid are either water and a water-soluble solvent or The solution is characterized by being dissolved in both.

In order to solve the above problem, sterilizing solution producing apparatus of the present invention, water, and, in either or both of the water-soluble solvent, to accommodate the compound dissolved water is a solution prepared by dissolving chloroperbenzoic acid reservoir In the tank, the ozone generation part which produces | generates ozone, and the bubbling part which produces | generates a bactericidal solution by bubbling ozone to compound solution water in the storage tank, It was characterized by the above-mentioned.
In order to solve the above-mentioned problem, another sterilizing solution manufacturing apparatus of the present invention is a compound-dissolved water which is a solution in which m-chloroperbenzoic acid is dissolved in either or both of water and a water-soluble solvent. And a bubbling unit that generates a bactericidal solution by bubbling ozone into the compound-dissolved water in the storage tank.

In order to solve the above problems, other sterilizing solution producing apparatus of the present invention, an ozone generator for generating ozone, water, and, in either or both of the water-soluble solvent, dissolving the chloroperbenzoic acid It is characterized by comprising an ejector that dissolves ozone in the compound-dissolved water that is a solution, and a storage tank that contains a sterilizing solution in which ozone is dissolved in the compound-dissolved water.
In order to solve the above-mentioned problems, another sterilizing solution manufacturing apparatus of the present invention includes m-chloroperbenzoic acid in one or both of an ozone generator that generates ozone, water, and a water-soluble solvent. An ejector that dissolves ozone in compound-dissolved water, which is a dissolved solution, and a storage tank that stores a sterilizing solution in which ozone is dissolved in compound-dissolved water.

  A concentration measuring unit that measures the ozone concentration in the sterilizing solution stored in the storage tank and an ozone control unit that adjusts the amount of ozone generated by the ozone generating unit based on the measured ozone concentration Good.

  According to the present invention, sterilization efficiency can be improved in sterilization using ozone.

It is a figure for demonstrating the mechanism of the improvement of a bactericidal effect. It is a figure for demonstrating a disinfection solution manufacturing apparatus. It is a figure for demonstrating the bactericidal solution manufacturing apparatus concerning the modification 1. FIG. It is a figure for demonstrating the bactericidal solution manufacturing apparatus concerning the modification 2. FIG.

  Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The dimensions, materials, and other specific numerical values shown in the embodiments are merely examples for facilitating the understanding of the invention, and do not limit the present invention unless otherwise specified. In the present specification and drawings, elements having substantially the same function and configuration are denoted by the same reference numerals, and redundant description is omitted, and elements not directly related to the present invention are not illustrated. To do.

  A sterilizing solution for sterilizing an object (for example, an endoscope) according to the present embodiment includes ozone, perbenzoic acids, and one or both of benzoic acids, water, and a water-soluble solvent. It is a solution dissolved in either one or both. Here, as a sterilizing solution, a solution in which ozone, perbenzoic acid, and / or a compound of benzoic acid is dissolved in water will be described as an example. However, ozone and perbenzoic acid are described. , And a compound of either one or both of benzoic acids may be dissolved in a water-soluble solvent (for example, ethanol, acetic acid) in addition to or in place of water.

  Benzoic acids include, for example, benzoic acid, methyl 4-hydroxybenzoate, ethyl 4-hydroxybenzoate, propyl 4-hydroxybenzoate, isopropyl 4-hydroxybenzoate, butyl paraben. Paraoxybenzoic acid esters such as hydroxybenzoate), isobutyl 4-hydroxybenzoate, benzyl 4-hydroxybenzoate and the like may be used. Benzoic acids include o-fluorobenzoic acid, m-fluorobenzoic acid, p-fluorobenzoic acid, o-chlorobenzoic acid, m-chlorobenzoic acid, p-chlorobenzoic acid, o-bromobenzoic acid, m- Halogenated benzoic acid such as bromobenzoic acid and p-bromobenzoic acid may be used, and 2-amino-4-chlorobenzoic acid and 4-chloro-3-nitrobenzoic acid may be used. Furthermore, the benzoic acid may be a halogenated benzoic acid ester such as a fluoroparaoxybenzoic acid ester, a chloroparaoxybenzoic acid ester, or a bromoparaoxybenzoic acid ester.

  Perbenzoic acids include, for example, perbenzoic acid, o-fluoroperbenzoic acid, m-fluoroperbenzoic acid, p-fluoroperbenzoic acid, o-chloroperbenzoic acid, m-chloroperbenzoic acid, p-chloroperbenzoic acid. Halogenated perbenzoic acid such as benzoic acid, o-bromoperbenzoic acid, m-bromoperbenzoic acid, and p-bromoperbenzoic acid may be used.

  The inventor of the present application provides a case where only ozone is present in water by allowing one or both of perbenzoic acid and a compound of benzoic acid to be present in water together with ozone, or perbenzoic acid and benzoic acid. It has been found that the bactericidal effect is improved as compared with the case where only one or both of these compounds are present in water.

FIG. 1 is a diagram for explaining a mechanism for improving the bactericidal effect. When the present inventor coexists perbenzoic acids or benzoic acids with ozone in water, the oxidative power of ozone causes hydroxide ions (FIG. 1 (a However, it was speculated that hydroxide ions (see FIG. 1 (b)) would be liberated from paraoxybenzoic acid esters and hydroxide ions (see FIG. 1 (c)) from perbenzoic acid. And it estimated that the free hydroxide ion (OH < ^- >) and ozone reacted further, and a hydroxy radical (OH *) was produced | generated from hydroxide ion. Thus, since the produced | generated hydroxyl radical liberates a hydroxide ion from a compound or makes a hydroxide ion into a hydroxyl radical, it is estimated that a hydroxyl radical can be produced | generated in a chain.

  In addition, peracids such as perbenzoic acid are unstable substances, and generate hydrogen peroxide when decomposed to become stable (no oxidizing power) substances such as benzoic acid. At this time, in the presence of ozone, hydrogen peroxide becomes a hydroxy radical and exhibits oxidizing power. It is known that a substance that is not a peracid (for example, benzoic acid) after generating and decomposing hydrogen peroxide becomes a peracid again in the presence of ozone. Thus, the presence of ozone causes perbenzoic acid to repeatedly change from benzoic acid → perbenzoic acid → benzoic acid. Therefore, hydrogen peroxide can be continuously generated in the process of repeated change of benzoic acid and perbenzoic acid, and hydroxy radicals can be stably generated from the hydrogen peroxide. In such a system in which ozone is constantly supplied from the outside, a series of these reactions (repetitive changes between benzoic acid and perbenzoic acid) will occur continuously, and a stable oxidizing power can be exhibited. It becomes possible.

  Since the hydroxy radical has a bactericidal effect, as described above, by coexisting perbenzoic acid or benzoic acid with ozone in water, a hydroxy radical can be generated in a chain and the bactericidal effect can be improved. It becomes possible.

  Then, the sterilization solution manufacturing apparatus which manufactures the said sterilization solution is demonstrated.

(Sterilizing solution manufacturing apparatus 100)
FIG. 2 is a diagram for explaining the sterilizing solution manufacturing apparatus 100. As shown in FIG. 2, the sterilizing solution manufacturing apparatus 100 includes an oxygen cylinder 110, an ozone generation unit 114, a valve 116, a storage tank 120, a bubbling unit 130, an ozone decomposition unit 140, a valve 142, and a blower. 144. Here, m-chloroperbenzoic acid (hereinafter simply referred to as MCPBA) will be described as an example as a compound of one or both of perbenzoic acid and benzoic acid.

The ozone generator 114 is a so-called ozonizer, and forms a discharge space by applying a predetermined voltage. When oxygen (O ₂ ) is supplied from the oxygen cylinder 110 to this discharge space, oxygen is activated and becomes ozone (O ₃ ). The ozone generated by the ozone generator 114 is sent to the bubbling unit 130 through the valve 116.

  The storage tank 120 stores an MCPBA aqueous solution 122 (compound dissolved water) supplied by a supply unit (not shown). In this embodiment, the supply means supplies the MCPBA aqueous solution 122 to the storage tank 120. However, the MCPBA aqueous solution 122 is generated by supplying MCPBA and water in separate lines and mixing them in the storage tank 120. May be.

  The bubbling unit 130 bubbles ozone into the MCPBA aqueous solution 122 in the storage tank 120 (supplied as fine bubbles). With the configuration including the bubbling unit 130, ozone can be efficiently dissolved in the MCPBA aqueous solution 122. Thus, it becomes possible to produce a sterilizing solution in which MCPBA and ozone are dissolved in water.

  The ozone decomposition unit 140 is composed of a catalyst that decomposes ozone, and ozone that has not been dissolved in the MCPBA aqueous solution 122 is introduced from the storage tank 120 through the valve 142 to decompose ozone. Exhaust gas generated by decomposing ozone by the ozone decomposing unit 140 is discharged to the outside by the blower 144.

  As described above, according to the sterilizing solution manufacturing apparatus 100 according to the present embodiment, a sterilizing solution having a high sterilizing effect can be efficiently manufactured.

  In the sterilizing solution manufacturing apparatus 100 shown in FIG. 2, ozone is dissolved in the MCPBA aqueous solution 122 in the storage tank 120, but ozone is added to the MCPBA aqueous solution 122 between the ozone generator 114 and the valve 116. It may be dissolved.

(Modification 1)
FIG. 3 is a view for explaining a sterilizing solution manufacturing apparatus 200 according to the first modification. As shown in FIG. 3, the sterilizing solution manufacturing apparatus 200 includes an oxygen generation unit 210, a pump 212, an ozone generation unit 114, a valve 116, a storage tank 120, a bubbling unit 130, an ozone decomposition unit 140, A valve 142 and a blower 144 are included. The ozone generation unit 114, the valve 116, the storage tank 120, the bubbling unit 130, the ozone decomposition unit 140, the valve 142, and the blower 144 that have already been described as the constituent elements of the sterilizing solution manufacturing apparatus 100 described above have substantially the same functions. Therefore, overlapping description is omitted, and here, the oxygen generation unit 210 and the pump 212 having different configurations will be mainly described.

  The oxygen generator 210 is, for example, a PSA (Pressure Swing Adsorption) type oxygen separator, and collects oxygen by separating oxygen from the air pumped by the pump 212. The oxygen collected in the oxygen generator 210 is sent to the ozone generator 114.

  Thus, according to the sterilizing solution manufacturing apparatus 200 of Modification 1, oxygen (ozone) can be generated from the air without the oxygen cylinder 110, so that the cost required for oxygen can be reduced. Become.

  In the sterilizing solution manufacturing apparatus 200 shown in FIG. 3, ozone is dissolved in the MCPBA aqueous solution 122 in the storage tank 120, but ozone is added to the MCPBA aqueous solution 122 between the ozone generator 114 and the valve 116. It may be dissolved.

(Modification 2)
FIG. 4 is a diagram for explaining the sterilizing solution manufacturing apparatus 300 according to the second modification. As shown in FIG. 4, the sterilizing solution manufacturing apparatus 300 includes an oxygen cylinder 110, an ozone generation unit 114, a pump 310, an ejector 312, a concentration measurement unit 320, an ozone control unit 330, a storage tank 120, The ozonolysis part 140, the valve | bulb 142, and the blower 144 are comprised. Since the oxygen cylinder 110, the ozone generation unit 114, the storage tank 120, the ozone decomposition unit 140, the valve 142, and the blower 144, which have already been described as the constituent elements of the sterilizing solution manufacturing apparatus 100 described above, have substantially the same functions, they are redundantly described. Here, the pump 310, the ejector 312, the concentration measuring unit 320, and the ozone control unit 330 having different configurations will be mainly described.

  The pump 310 circulates the MCPBA aqueous solution 122 to the storage tank 120. In the present embodiment, the pump 310 sends the MCPBA aqueous solution 122 to two circulation paths 310b, which are a circulation path 310a where an ejector 312 described later is disposed and a circulation path where a concentration measuring unit 320 described later is disposed. The ejector 312 is provided on the circulation path 310 a of the MCPBA aqueous solution 122 by the pump 310, and dissolves ozone supplied from the ozone generator 114 in the MCPBA aqueous solution 122.

  The concentration measuring unit 320 is provided on the circulation path 310b of the MCPBA aqueous solution 122 by the pump 310, and measures the ozone concentration (dissolved ozone concentration) in the sterilizing solution flowing through the circulation path 310b.

  The ozone control unit 330 is composed of a semiconductor integrated circuit including a CPU (Central Processing Unit), reads programs and parameters for operating the CPU itself from the ROM, and cooperates with the RAM as a work area and other electronic circuits. Thus, the entire sterilizing solution manufacturing apparatus 300 is managed and controlled.

  In Modification 2, the ozone control unit 330 controls the ozone generation unit 114 based on the ozone concentration measured by the concentration measurement unit 320 and adjusts the amount of ozone generated by the ozone generation unit 114.

  Thus, the sterilizing solution manufacturing apparatus 300 according to Modification 2 can maintain the ozone concentration in the sterilizing solution at a concentration suitable for sterilization by the configuration including the concentration measuring unit 320 and the ozone control unit 330.

  In the sterilizing solution manufacturing apparatus 300 shown in FIG. 4, the configuration in which oxygen is supplied from the oxygen cylinder 110 to the ozone generation section 114 has been described. However, instead of the oxygen cylinder 110, an oxygen generation section 210 and a pump 212 are provided. In addition, oxygen may be supplied from the oxygen generation unit 210 and the pump 212 to the ozone generation unit 114.

Example 1
Bactericidal efficiency was tested using a bactericidal solution having a dissolved ozone concentration of 0.5 mg / L and MCPBA of 0.0075% by mass. At this time, Mycobacterium terrae ATCC15755 was used as an indicator bacterium. Test system A counts the number of bacteria in the suspension to which 0.1% of serum as a soil substance was added, and test system B contains 0.03% by mass of BSA (bovine serum albumin) as a soil substance. The number of bacteria on the added carrier was counted. In addition, the temperature of the sterilizing solution was 20 ° C., and the treatment time (the time during which the sterilizing solution and the bacteria were contacted) was 5 minutes.

As shown in Table 1 below, in Test System A, the number of bacteria decreased from 2.0 × 10 ⁶ cfu / mL to 0 (zero). In the test system B, the number of bacteria decreased from 6.0 × 10 ⁶ cfu / mL to 0 (zero). That is, in Example 1, it was found that the number of bacteria can be reduced by 6 orders of magnitude or more simply by adding an extremely small amount of MCPBA such as 0.0075% by mass to ozone at a very low concentration of 0.5 mg / L. .

(Example 2)
In Example 2, 0.01 g of MCPBA was dissolved in 200 μL of ethanol and added to 200 mL of an aqueous solution having a dissolved ozone concentration of 0.5 mg / L to produce a sterilizing solution. Further, as Comparative Example 1, an aqueous solution in which only ozone was dissolved (200 mL of an aqueous solution having a dissolved ozone concentration of 0.5 mg / L) was generated, and as Comparative Example 2, an aqueous solution in which only MCPBA was dissolved (0.00005 g / mL) was generated. did.

^Also, so that 10 6 cfu / cm ^2, to create what was fixed indicator bacteria (Mycobacterium terrae ATCC15755) to the test piece. Then, the sterilizing solution at 20 ° C., an aqueous solution in which only ozone of Comparative Example 1 is dissolved (hereinafter referred to as an aqueous ozone solution), an aqueous solution in which only MCPBA of Comparative Example 2 is dissolved (hereinafter referred to as an MCPBA aqueous solution), The test piece on which the indicator bacteria were fixed was immersed for 5 minutes.

  As shown in Table 2 below, when the test piece was immersed in an aqueous ozone solution as Comparative Example 1, the logarithmic decrease value of the number of bacteria was only 1.9. Moreover, when the test piece was immersed in the MCPBA aqueous solution as Comparative Example 2, the logarithmic decrease value of the number of bacteria was only 1.5.

On the other hand, as Example 2, when the test piece was immersed in the sterilizing solution (aqueous solution in which ozone and MCPBA were dissolved), the logarithmic decrease value of the number of bacteria was 3.5. In other words, compared to Comparative Examples 1 and 2, in Example 2, it was found that the number of bacteria can be reduced by 0.1 logarithm. Therefore, compared with the comparative example 1 and the comparative example 2 which were performed conventionally, in Example 2, it turned out that sterilization efficiency improves remarkably.

  Moreover, this inventor performed the deterioration test of material with the ozone aqueous solution and the disinfection solution, using polyurethane as a material. As a result, it was confirmed that no material deterioration was observed in both cases. Therefore, it has been found that even when an object such as an endoscope is sterilized using the sterilizing solution according to the present embodiment, the object can be sterilized without deteriorating.

  As mentioned above, although preferred embodiment of this invention was described referring an accompanying drawing, it cannot be overemphasized that this invention is not limited to this embodiment. It will be apparent to those skilled in the art that various changes and modifications can be made within the scope of the claims, and these are naturally within the technical scope of the present invention. Is done.

  In addition, the concentration measuring unit 320 and the ozone control unit 330 are provided in the sterilizing solution manufacturing apparatuses 100 and 200 described above, and the control unit 330 controls the ozone generating unit 114 based on the ozone concentration measured by the concentration measuring unit 320. The amount of ozone generated by the ozone generation unit 114 may be controlled. Thereby, also in the sterilization solution manufacturing apparatuses 100 and 200, the ozone concentration in the sterilization solution can be maintained at a concentration suitable for sterilization.

  The present invention can be used for a sterilizing solution for sterilizing an object and a sterilizing solution manufacturing apparatus.

100, 200, 300 ... sterilizing solution manufacturing apparatus 114 ... ozone generation part 120 ... storage tank 130 ... bubbling part 312 ... ejector

Claims (7)

A sterilizing solution for sterilizing an object,
With ozone,
Chloro and perbenzoic acid,
A sterilizing solution, which is a solution in which water is dissolved in either or both of water and a water-soluble solvent.   A sterilizing solution for sterilizing an object,
  With ozone,
  m-chloroperbenzoic acid,
A sterilizing solution, which is a solution in which water is dissolved in either or both of water and a water-soluble solvent. Water, and, in either or both of the water-soluble solvent, a reservoir for containing the compounds dissolved water is a solution prepared by dissolving chloroperbenzoic acid,
An ozone generator for generating ozone;
In the storage tank, by bubbling the ozone in the compound-dissolved water, a bubbling unit that generates a sterilizing solution;
An apparatus for producing a sterilizing solution, comprising:   A reservoir containing compound-dissolved water which is a solution in which m-chloroperbenzoic acid is dissolved in either or both of water and a water-soluble solvent;
  An ozone generator for generating ozone;
  In the storage tank, by bubbling the ozone in the compound-dissolved water, a bubbling unit that generates a sterilizing solution;
An apparatus for producing a sterilizing solution, comprising: An ozone generator for generating ozone;
Water, and, in either or both of the water-soluble solvent, the compound dissolved water is a solution prepared by dissolving chloroperbenzoic acid, and an ejector for dissolving the ozone,
A storage tank containing a sterilizing solution in which ozone is dissolved in the compound-dissolved water;
An apparatus for producing a sterilizing solution, comprising:   An ozone generator for generating ozone;
  An ejector for dissolving ozone in compound-dissolved water, which is a solution in which m-chloroperbenzoic acid is dissolved in either or both of water and a water-soluble solvent;
  A storage tank containing a sterilizing solution in which ozone is dissolved in the compound-dissolved water;
An apparatus for producing a sterilizing solution, comprising: A concentration measuring unit for measuring the ozone concentration in the sterilizing solution contained in the storage tank;
Based on the measured ozone concentration, an ozone control unit that adjusts the amount of ozone generated by the ozone generation unit;
The sterilizing solution manufacturing apparatus according to any one of claims 3 to 6 , further comprising:

Images