JP5847074B2 - 基板上対照検出 - Google Patents
基板上対照検出 Download PDFInfo
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Description
本願は、参照によりその全体が本願に組み込まれる、米国仮出願第61/170,440号明細書(2009年4月17日出願、発明の名称「電気化学的基板上対照検出(ELECTROCHEMICAL ON−BOARD CONTROL DETECTION)」)の優先権を主張する。
図1は、単一のチャンバを備える例示的な電気化学的バイオセンサの分解図を示す。1は下部電極を示し、2は絶縁分離器またはスペーサ層を示す。スペーサ層には、その長さの一部に沿って、開口部がある。3は上部電極を示す。上部電極3は、その長さの一部に沿う第1の開口部、および第1の開口部とある角度をなす第2の開口部を有する。上部電極の第1の開口部は、スペーサ層2の開口部と実質的に平行である。4は、5で示される充填チャンバのカバーを示す。6は反応チャンバを示す。充填チャンバ5は、下部電極1、カバー4、上部電極3の開口部、およびスペーサ層2の開口部によって、画定される。反応チャンバ6もまた、下部電極1、カバー4、上部電極3、上部電極3の開口部、およびスペーサ層2の開口部によって画定される。対照システムおよび検査システムは、反応チャンバ6内に配置することができる。
図2は、2つのチャンバを備える例示的な電気化学的バイオセンサの分解図を示す。1は下部電極を示し、2は絶縁分離器またはスペーサ層を示す。スペーサ層には、その長さの一部に沿って、開口部がある。3は上部電極を示す。上部電極3は、その長さの一部に沿う第1の開口部、第1の開口部とある角度をなす第2の開口部、および第1の開口部とある角度をなして第2の開口部と実質的に平行な第3の開口部を有する。上部電極の第1の開口部は、スペーサ層2の開口部と実質的に平行である。4は、5で示される充填チャンバのカバーを示す。6は反応チャンバを示す。充填チャンバ5は、下部電極1、カバー4、上部電極3の開口部、およびスペーサ層2の開口部によって画定される。2つの反応チャンバ6もまた、下部電極1、カバー4、上部電極3、上部電極3の開口部、およびスペーサ層2の開口部によって画定される。2つの反応チャンバの間に試料の通路がある。対照システムおよび検査システムは、異なる反応チャンバ6内に配置することができる。
図3は、例示的な電気化学的バイオセンサを示す。1は下部電極を示し、絶縁分離器すなわちスペーサ層は同図には示されていない。3は上部電極を示す。4は、5で示される充填チャンバのカバーを示す。6は反応チャンバを示す。対照システムおよび検査システムは、反応チャンバ6内に配置することができる。
図4は、異なる保管条件の後の凝固バイオセンサを使用した測定の比較を示す。電流記録は、正常血液がバイオセンサに添加されたときに開始した。最初の約3秒間は、外部電圧はバイオセンサに印加されなかった。バイオセンサ自体によって生成された電流が測定された。約3秒後、定電位電解装置はバイオセンサに約0.3Vを印加し、その結果生じた電流を測定した。菱形で印を付けられた点は、試料が所定のアルゴリズムにしたがって凝固したと見なされる時間を表す。太い実線は、室温で保管された2つのバイオセンサを表す。細い点線は、約60℃で2週間保管された2つのバイオセンサを表す。
Claims (31)
- 試料を評価するためのセンサであって、基板上対照システムおよび検査システムを含んでおり、前記対照システムが、ヨウ素、アスコルビン酸、フェリシアン化物、フェロシアン化物、4−アミノ−2−クロロフェノール、またはこれらのいずれかの組合わせから選択される少なくとも1つの対照試薬を含み、前記少なくとも1つの対照試薬がN−オキシド化合物ではなく、かつニトロソ官能基を含まず、かつ前記対照システムが前記対照試薬に関わる対照反応を含み、前記対照反応が対照信号を生成し、前記対照信号が前記対照システムおよび前記検査システムのうちの少なくとも1つの生存能を示し、前記対照反応で用いられた前記対照試薬は前記検査システムの反応に対しては使用可能ではない、センサ。
- 前記対照システムが電気化学的対照システムを含む、請求項1に記載のセンサ。
- 前記対照反応が外部電圧を用いることなく評価される、請求項2に記載のセンサ。
- 前記対照反応が、前記少なくとも1つの試薬を試料と接触させると活性化され、前記接触が前記対照信号の生成を開始する、請求項1に記載のセンサ。
- 前記生成された対照信号が外部ストレスに依存し、前記外部ストレスが、温度、pH、湿度、酸素、光、保管寿命、事前の液体接触、および化学汚染物質から選択される少なくとも1つを含む、請求項4に記載のセンサ。
- 前記対照システムが少なくとも2つの電極を含み、前記少なくとも1つの試薬が前記電極のうちの少なくとも1つに被覆され、前記少なくとも2つの電極が同一平面内にある、または互いに対向している、請求項2に記載のセンサ。
- 前記対照反応が起電力を発生させる、請求項2に記載のセンサ。
- 前記起電力が、前記少なくとも1つの試薬の前記試料中への溶解をもとに発生される、請求項7に記載のセンサ。
- 前記少なくとも1つの試薬がフェリシアン化物を含む、請求項2に記載のセンサ。
- 前記対照システムが少なくとも2つの電極を含み、前記電極のうちの少なくとも1つに被覆されたフェリシアン化物の面積負荷が、1平方メートル当たり10×10−6から200×10−6モルである、請求項9に記載のセンサ。
- 前記少なくとも1つの試薬が中和剤を含み、前記中和剤が中和作用によって前記対照信号を中和する、請求項1に記載のセンサ。
- 前記中和作用が、化学反応および物理的効果から選択される少なくとも1つを含む、請求項11に記載のセンサ。
- 前記物理的効果が、沈殿および拡散から選択される少なくとも1つを含む、請求項12に記載のセンサ。
- 前記対照システムおよび前記検査システムが1つのチャンバ内に配置されている、または、前記対照システムが第1のチャンバ内に配置され、前記検査システムの少なくとも一部が第2のチャンバ内に配置されている、請求項1に記載のセンサ。
- 前記第1のチャンバおよび前記第2のチャンバが並列に流体接続または試料通路を介して直列に流体接続している、請求項14に記載のセンサ。
- センサを使用して試料を評価する方法であって、
基板上対照システムおよび検査システムを含む前記センサに前記試料を付着させることであって、前記対照システムが、ヨウ素、アスコルビン酸、フェリシアン化物、フェロシアン化物、4−アミノ−2−クロロフェノール、またはこれらのいずれかの組合わせから選択される少なくとも1つの対照試薬を含み、前記少なくとも1つの対照試薬がN−オキシド化合物ではなく、かつニトロソ官能基を含まず、かつ前記対照システムが前記対照試薬に関わる対照反応を含み、前記対照反応が対照信号を生成し、前記対照信号が前記対照システムおよび前記検査システムのうちの少なくとも1つの生存能を示し、前記対照反応で用いられた前記対照試薬は前記検査システムの反応に対しては使用可能ではない、付着させることと、
前記対照システムおよび前記検査システムのうちの少なくとも1つの生存能を判定するために、前記対照信号を標準信号と比較することと
を含む方法。 - 前記検査システム内の前記試料を評価することをさらに含む、請求項16に記載の方法。
- 前記対照システムは、電気化学的対照システムを含む、請求項16に記載の方法。
- 前記対照反応は、外部電圧を用いることなく評価される、請求項18に記載の方法。
- 前記対照反応は、前記少なくとも1つの試薬を試料と接触させると活性化され、前記接触は前記対照信号の生成を開始する、請求項16に記載の方法。
- 前記対照システムは、少なくとも2つの電極を含むとともに前記少なくとも1つの試薬は前記電極のうちの少なくとも1つに被覆され、前記少なくとも2つの電極が同一平面内にある、または互いに対向している、請求項18に記載の方法。
- 前記対照反応は、起電力を発生させる、請求項18に記載の方法。
- 前記起電力は、前記少なくとも1つの試薬の前記試料中への溶解をもとに発生される、請求項22に記載の方法。
- 前記少なくとも1つの試薬は、フェリシアン化物を含む、請求項18に記載の方法。
- 前記少なくとも1つの試薬は、中和剤を含むとともに前記中和剤は中和作用によって前記対照信号を中和する、請求項16に記載の方法。
- 前記中和作用は、化学反応および物理的効果から選択される少なくとも1つを含む、請求項25に記載の方法。
- 前記対照システムおよび前記検査システムは、1つのチャンバ内に配置されている、または、前記対照システムが第1のチャンバ内に配置され、前記検査システムの少なくとも一部が第2のチャンバ内に配置されている、請求項16に記載の方法。
- 前記第1のチャンバおよび前記第2のチャンバは、並列に流体接続または試料通路を介して直列に流体接続している、請求項27に記載の方法。
- 前記試料を前記第1のチャンバから前記第2のチャンバへ毛細管現象により移送するステップをさらに含む、請求項28に記載の方法。
- 前記検査システム内の前記試料を評価することは、免疫反応および電気化学反応から選択される少なくとも1つの反応を測定することを含む、請求項17に記載の方法。
- 前記検査システム内の前記試料を評価することは、電気信号および光信号から選択される少なくとも1つの検査信号を測定することを含む、請求項17に記載の方法。
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