JP5827220B2 - 人工多能性幹細胞の樹立効率改善方法 - Google Patents

人工多能性幹細胞の樹立効率改善方法 Download PDF

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JP5827220B2
JP5827220B2 JP2012513400A JP2012513400A JP5827220B2 JP 5827220 B2 JP5827220 B2 JP 5827220B2 JP 2012513400 A JP2012513400 A JP 2012513400A JP 2012513400 A JP2012513400 A JP 2012513400A JP 5827220 B2 JP5827220 B2 JP 5827220B2
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伸弥 山中
伸弥 山中
誠人 中川
誠人 中川
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JP2012513400A 2010-01-22 2011-01-21 人工多能性幹細胞の樹立効率改善方法 Active JP5827220B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28232010P 2010-01-22 2010-01-22
US61/282,320 2010-01-22
PCT/JP2011/051685 WO2011090221A1 (en) 2010-01-22 2011-01-21 Method for improving induced pluripotent stem cell generation efficiency

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JP2013517758A JP2013517758A (ja) 2013-05-20
JP2013517758A5 JP2013517758A5 (OSRAM) 2014-03-06
JP5827220B2 true JP5827220B2 (ja) 2015-12-02

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US (1) US9005967B2 (OSRAM)
EP (1) EP2526189B1 (OSRAM)
JP (1) JP5827220B2 (OSRAM)
WO (1) WO2011090221A1 (OSRAM)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102703637B1 (ko) 2010-12-22 2024-09-05 페이트 세러퓨틱스, 인코포레이티드 단세포 분류 및 iPSC의 증강된 재프로그래밍을 위한 세포 배양 플랫폼
JP6426110B2 (ja) 2013-02-01 2018-11-21 ザ ユナイテッド ステイツ オブ アメリカ， アズ リプレゼンテッド バイ ザ セクレタリー， デパートメント オブ ヘルス アンド ヒューマン サービシーズ 人口多能性幹細胞（ｉｐｓｃ）由来の網膜色素上皮（ｒｐｅ）細胞を生成するための方法
DK3766962T3 (da) 2013-02-06 2025-11-17 Univ Rochester Inducerede, pluripotente, celleafledte oligodendrocyt-progenitorceller til behandling af myelinforstyrrelser
KR102340553B1 (ko) * 2014-03-04 2021-12-21 페이트 세러퓨틱스, 인코포레이티드 개선된 재프로그래밍 방법 및 세포 배양 플랫폼
US9724432B2 (en) 2015-04-30 2017-08-08 University Of Rochester Non-human mammal model of human degenerative disorder, uses thereof, and method of treating human degenerative disorder
AU2016318774B2 (en) 2015-09-08 2022-08-18 FUJIFILM Cellular Dynamics, Inc. MACS-based purification of stem cell-derived retinal pigment epithelium
JP6983762B2 (ja) 2015-09-08 2021-12-17 ザ ユナイテッド ステイツ オブ アメリカ， アズ リプレゼンテッド バイ ザ セクレタリー， デパートメント オブ ヘルス アンド ヒューマン サービシーズ 臨床グレードの網膜色素上皮細胞の再現性のある分化のための方法
AU2016338680B2 (en) 2015-10-16 2022-11-17 Fate Therapeutics, Inc. Platform for the induction and maintenance of ground state pluripotency
US10947502B2 (en) 2015-10-20 2021-03-16 FUJIFILM Cellular Dynamics, Inc. Methods for directed differentiation of pluripotent stem cells to immune cells
EP3478325B1 (en) 2016-07-01 2024-07-17 Research Development Foundation Elimination of proliferating cells from stem cell-derived grafts
WO2018026723A1 (en) 2016-08-01 2018-02-08 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Human induced pluripotent stem cells for high efficiency genetic engineering
WO2018067826A1 (en) 2016-10-05 2018-04-12 Cellular Dynamics International, Inc. Generating mature lineages from induced pluripotent stem cells with mecp2 disruption
JP7222900B2 (ja) 2016-11-09 2023-02-15 ザ ユナイテッド ステイツ オブ アメリカ アズ リプリゼンテッド バイ ザ セクレタリー、デパートメント オブ ヘルス アンド ヒューマン サービシーズ 細胞療法および創薬のための３ｄ血管化ヒト眼組織
JP7203427B2 (ja) 2017-02-14 2023-01-13 ユニバーシティ オブ ピッツバーグ － オブ ザ コモンウェルス システム オブ ハイヤー エデュケイション ヒト人工多能性幹細胞を操作して肝臓組織を作製する方法
IL269716B2 (en) 2017-04-18 2025-07-01 Fujifilm Cellular Dynamics Inc Antigen-specific immune effector cells
CN110913689A (zh) 2017-05-10 2020-03-24 罗切斯特大学 治疗神经精神病症的方法
US12110503B2 (en) 2018-04-20 2024-10-08 FUJIFILM Cellular Dynamics, Inc. Method for differentiation of ocular cells and use thereof
WO2019246112A1 (en) 2018-06-18 2019-12-26 University Of Rochester Methods of treating schizophrenia and other neuropsychiatric disorders
CA3103675A1 (en) 2018-06-21 2019-12-26 University Of Rochester Methods of treating or inhibiting onset of huntington's disease
EP3818158B1 (en) * 2018-07-02 2025-10-08 Shi-Lung Lin In-vitro induction of adult stem cell expansion and derivation
CA3119041A1 (en) 2018-11-19 2020-05-28 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Biodegradable tissue replacement implant and its use
CA3121027A1 (en) 2018-11-28 2020-06-04 Board Of Regents, The University Of Texas System Multiplex genome editing of immune cells to enhance functionality and resistance to suppressive environment
WO2020112493A1 (en) 2018-11-29 2020-06-04 Board Of Regents, The University Of Texas System Methods for ex vivo expansion of natural killer cells and use thereof
CN113728103B (zh) 2018-12-11 2025-02-28 罗切斯特大学 治疗精神分裂症和其他神经精神病症的方法
US20230057355A1 (en) 2019-02-13 2023-02-23 University Of Rochester Gene networks that mediate remyelination of the human brain
EP4118234A1 (en) 2020-03-09 2023-01-18 FUJIFILM Corporation Markers specific for pluripotent stem cells, and methods of using the same
CA3178949A1 (en) 2020-05-29 2021-12-02 Andrew DIAS Retinal pigmented epithelium and photoreceptor dual cell aggregates and methods of use thereof
WO2021243203A1 (en) 2020-05-29 2021-12-02 FUJIFILM Cellular Dynamics, Inc. Bilayer of retinal pigmented epithelium and photoreceptors and use thereof
CA3201499A1 (en) 2020-11-13 2022-05-19 Catamaran Bio, Inc. Genetically modified natural killer cells and methods of use thereof
EP4247938A4 (en) 2020-11-18 2024-05-15 FUJIFILM Corporation Generation of secretome-containing compositions, and methods of using and analyzing the same
AU2021388074A1 (en) 2020-11-24 2023-06-22 Monash University Methods and cellular structures
US12492372B2 (en) 2020-11-24 2025-12-09 Monash University Induced stem cells
WO2022115611A1 (en) 2020-11-25 2022-06-02 Catamaran Bio, Inc. Cellular therapeutics engineered with signal modulators and methods of use thereof
CA3214490A1 (en) 2021-03-25 2022-09-29 Bluerock Therapeutics Lp Methods for obtaining induced pluripotent stem cells
CA3220168A1 (en) 2021-05-26 2022-12-01 Sarah DICKERSON Methods to prevent rapid silencing of genes in pluripotent stem cells
EP4347795A1 (en) 2021-05-28 2024-04-10 The United States of America, as represented by The Secretary, Department of Health and Human Services Methods to generate macular, central and peripheral retinal pigment epithelial cells
WO2022251477A1 (en) 2021-05-28 2022-12-01 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Biodegradable tissue scaffold with secondary matrix to host weakly adherent cells
KR20240056604A (ko) 2021-09-13 2024-04-30 후지필름 셀룰러 다이내믹스, 인코포레이티드 수임 심장 전구세포의 제조 방법
EP4419655A1 (en) 2021-10-20 2024-08-28 University of Rochester Humanized chimeras for the prospective assessment of cell addition and replacement therapies
CA3234404A1 (en) 2021-10-20 2023-04-27 Steven A. Goldman Treatment with genetically modified cells, and genetically modified cells per se, with increased competitive advantage and/or decreased competitive disadvantage
WO2023069987A1 (en) 2021-10-20 2023-04-27 University Of Rochester Rejuvenation treatment of age-related white matter loss cross reference to related application
WO2023081633A1 (en) 2021-11-02 2023-05-11 University Of Rochester Tcf7l2 mediated remyelination in the brain
EP4472648A1 (en) 2022-02-01 2024-12-11 University of Rochester Methods of generating a population of neurons from human glial progenitor cells and genetic constructs for carrying out such methods
WO2023172514A1 (en) 2022-03-07 2023-09-14 Catamaran Bio, Inc. Engineered immune cell therapeutics targeted to her2 and methods of use thereof
US20250276020A1 (en) 2022-05-05 2025-09-04 University Of Rochester Dual macroglial-microglial approach towards therapeutic cell replacement in neurodegenerative and neuropsychiatric disease
WO2024006911A1 (en) 2022-06-29 2024-01-04 FUJIFILM Holdings America Corporation Ipsc-derived astrocytes and methods of use thereof
WO2024073776A1 (en) 2022-09-30 2024-04-04 FUJIFILM Cellular Dynamics, Inc. Methods for the production of cardiac fibroblasts
WO2024163747A2 (en) 2023-02-02 2024-08-08 University Of Rochester Competitive replacement of glial cells
WO2024192329A1 (en) 2023-03-16 2024-09-19 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods for producing stable human chondroctyes and their use for promoting cartillage growth and repair
US20250362218A1 (en) 2024-05-21 2025-11-27 FUJIFILM Cellular Dynamics, Inc. Particle counting and biomass measurements of aggregated cell compositions

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4937190A (en) 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
AU4336499A (en) 1998-06-08 1999-12-30 Osiris Therapeutics, Inc. Regulation of hematopoietic stem cell differentiation by the use of human mesenchymal stem cells
EP1186667B1 (en) 1999-05-18 2007-07-18 Dnavec Research Inc. Envelope gene-deficient virus vector of paramyxoviridae
US6602711B1 (en) 2000-02-21 2003-08-05 Wisconsin Alumni Research Foundation Method of making embryoid bodies from primate embryonic stem cells
JP3660601B2 (ja) 2001-03-30 2005-06-15 独立行政法人科学技術振興機構 胚性幹細胞からの神経幹細胞、運動ニューロン及びｇａｂａ作動性ニューロンの製造法
JP2004121165A (ja) 2002-10-07 2004-04-22 Asahi Kasei Corp 膵幹様細胞の分化誘導方法
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
US8048999B2 (en) 2005-12-13 2011-11-01 Kyoto University Nuclear reprogramming factor
EP2137296A2 (en) 2007-03-23 2009-12-30 Wisconsin Alumni Research Foundation Somatic cell reprogramming
CA2660123C (en) 2007-10-31 2017-05-09 Kyoto University Nuclear reprogramming method

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EP2526189B1 (en) 2016-10-12
US20120276636A1 (en) 2012-11-01
EP2526189A4 (en) 2014-03-19
WO2011090221A1 (en) 2011-07-28
EP2526189A1 (en) 2012-11-28
US9005967B2 (en) 2015-04-14
JP2013517758A (ja) 2013-05-20

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