JP5782712B2 - 心疾患マーカー標品およびその製造法 - Google Patents
心疾患マーカー標品およびその製造法 Download PDFInfo
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- JP5782712B2 JP5782712B2 JP2010292581A JP2010292581A JP5782712B2 JP 5782712 B2 JP5782712 B2 JP 5782712B2 JP 2010292581 A JP2010292581 A JP 2010292581A JP 2010292581 A JP2010292581 A JP 2010292581A JP 5782712 B2 JP5782712 B2 JP 5782712B2
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Description
2wt%のカゼイン系タンパク質を含む20mMトリス塩酸緩衝液(pH7.5)に、最終濃度がそれぞれ40pg/mL、0.5ng/mL、50ng/mL又は10ng/mLとなるようにBNP、cTnI、ミオグロビン及びCKMBを添加して、溶液Aを調整した。この溶液Aに対して、最終濃度がそれぞれ5mM又は10mMになるようにベンズアミジン塩酸塩を添加して溶液B及び溶液Cを調整した。
5wt%の牛血清アルブミンを含む20mMトリス塩酸緩衝液(pH7.5)に、最終濃度がそれぞれ100pg/mL、5ng/mL、100ng/mL又は100ng/mLとなるようにBNP、cTnI、ミオグロビン及びCKMBを添加して、溶液Dを調製した。ゲリセートペプトンを含む20mMトリス塩酸緩衝液(pH7.5)に対し同様にして溶液Eを、カゼイン系タンパク質を含む20mMトリス塩酸緩衝液(pH7.5)に対し同様にして溶液Fを、それぞれ調製した。
℃で7日間保存した。その後、凍結乾燥物に蒸留水2mLを加えて溶解しBNP、トロポニンI、ミオグロビン、CKMB濃度を測定した。結果を表2に示す。表2中、「濃度百分率」は、以下の式2に基づいて計算したものである。
Claims (2)
- カゼイン系タンパク質及びベンズアミジン誘導体を共存させたことを特徴とする心筋トロポニン及び脳性ナトリウム利尿ペプチド含有標品。
- カゼイン系タンパク質及びベンズアミジン誘導体を添加することを特徴とする、心筋トロポニン及び脳性ナトリウム利尿ペプチド含有標品の製造方法。
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JP2010292581A JP5782712B2 (ja) | 2010-12-28 | 2010-12-28 | 心疾患マーカー標品およびその製造法 |
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JP2010292581A JP5782712B2 (ja) | 2010-12-28 | 2010-12-28 | 心疾患マーカー標品およびその製造法 |
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JP2012141171A JP2012141171A (ja) | 2012-07-26 |
JP5782712B2 true JP5782712B2 (ja) | 2015-09-24 |
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JP2010292581A Active JP5782712B2 (ja) | 2010-12-28 | 2010-12-28 | 心疾患マーカー標品およびその製造法 |
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Families Citing this family (1)
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JP6008645B2 (ja) * | 2012-08-02 | 2016-10-19 | 株式会社Lsiメディエンス | Bnp測定用標準物質とその利用 |
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FR2701954B1 (fr) * | 1993-02-23 | 1995-07-07 | Pasteur Sanofi Diagnostics | Composition stabilisée de troponine pour immunoessais et procédé de stabilisation de troponine pour immunoessais. |
WO1998054219A1 (en) * | 1997-05-29 | 1998-12-03 | Medical Analysis Systems Inc. | Covalently coupled troponin complexes |
US20060183681A1 (en) * | 2005-02-14 | 2006-08-17 | Bio-Rad Laboratories, Inc. | Stabilized compositions containing natriuretic peptides |
JP2010091398A (ja) * | 2008-10-08 | 2010-04-22 | Tosoh Corp | 安定なナトリウム利尿ペプチド組成物 |
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