JP5773393B2 - 効率的な人工多能性幹細胞の樹立方法 - Google Patents

効率的な人工多能性幹細胞の樹立方法 Download PDF

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JP5773393B2
JP5773393B2 JP2012510468A JP2012510468A JP5773393B2 JP 5773393 B2 JP5773393 B2 JP 5773393B2 JP 2012510468 A JP2012510468 A JP 2012510468A JP 2012510468 A JP2012510468 A JP 2012510468A JP 5773393 B2 JP5773393 B2 JP 5773393B2
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cells
cell
lin28b
nucleic acid
ips
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JP2013505701A (ja
JP2013505701A5 (enExample
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伸弥 山中
伸弥 山中
剛士 田邊
剛士 田邊
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Kyoto University NUC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/604—Klf-4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/608—Lin28
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Transplantation (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2012510468A 2009-09-24 2010-09-22 効率的な人工多能性幹細胞の樹立方法 Active JP5773393B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US24547809P 2009-09-24 2009-09-24
US61/245,478 2009-09-24
PCT/JP2010/067016 WO2011037270A1 (en) 2009-09-24 2010-09-22 Method of efficiently establishing induced pluripotent stem cells

Publications (3)

Publication Number Publication Date
JP2013505701A JP2013505701A (ja) 2013-02-21
JP2013505701A5 JP2013505701A5 (enExample) 2013-11-07
JP5773393B2 true JP5773393B2 (ja) 2015-09-02

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JP2012510468A Active JP5773393B2 (ja) 2009-09-24 2010-09-22 効率的な人工多能性幹細胞の樹立方法

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US (1) US8993329B2 (enExample)
EP (1) EP2480657B1 (enExample)
JP (1) JP5773393B2 (enExample)
WO (1) WO2011037270A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014037574A1 (en) * 2012-09-10 2014-03-13 Sanofi Methods for reprogramming a somatic cell
CA3035660A1 (en) 2016-09-06 2018-03-15 The Children's Medical Center Corporation Immune cells derived from induced pluripotent stem cell
US20200190475A1 (en) * 2017-02-23 2020-06-18 San Diego State University (SDSU) Foundation, dba San Diego State University Research Foundation Methods for identifying and isolating cardiac stem cells and methods for making and using them
WO2018232079A1 (en) 2017-06-14 2018-12-20 Daley George Q Hematopoietic stem and progenitor cells derived from hemogenic endothelial cells by episomal plasmid gene transfer
MX2022008648A (es) 2020-01-23 2022-12-15 The Children´S Medical Center Corp Diferenciacion de celulas t sin estroma a partir de celulas madre pluripotentes humanas.
WO2022109667A1 (en) 2020-11-24 2022-06-02 Monash University Methods and cellular structures
JP2023550180A (ja) 2020-11-24 2023-11-30 モナシュ ユニバーシティ 誘導幹細胞

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4937190A (en) 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
ATE367445T1 (de) 1999-05-18 2007-08-15 Dnavec Research Inc Viraler paramyxoviridae vektor mit einem defekten hüllprotein-gen
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
US8278104B2 (en) * 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
KR101420740B1 (ko) 2005-12-13 2014-07-17 교또 다이가꾸 핵초기화 인자
EP2137296A2 (en) 2007-03-23 2009-12-30 Wisconsin Alumni Research Foundation Somatic cell reprogramming
KR101564044B1 (ko) 2007-10-31 2015-10-28 고쿠리츠 다이가쿠 호진 교토 다이가쿠 핵초기화 방법
JP5626619B2 (ja) 2008-12-08 2014-11-19 国立大学法人京都大学 効率的な核初期化方法
US9683232B2 (en) 2007-12-10 2017-06-20 Kyoto University Efficient method for nuclear reprogramming
WO2009096049A1 (ja) 2008-02-01 2009-08-06 Kyoto University 人工多能性幹細胞由来分化細胞
WO2011016588A1 (en) 2009-08-07 2011-02-10 Kyoto University Method of efficiently establishing induced pluripotent stem cells

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Publication number Publication date
JP2013505701A (ja) 2013-02-21
EP2480657A4 (en) 2013-09-25
EP2480657B1 (en) 2018-01-17
WO2011037270A1 (en) 2011-03-31
US20120214243A1 (en) 2012-08-23
EP2480657A1 (en) 2012-08-01
US8993329B2 (en) 2015-03-31

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