JP5763652B2 - 磁性サンプルの精製 - Google Patents
磁性サンプルの精製 Download PDFInfo
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- JP5763652B2 JP5763652B2 JP2012532688A JP2012532688A JP5763652B2 JP 5763652 B2 JP5763652 B2 JP 5763652B2 JP 2012532688 A JP2012532688 A JP 2012532688A JP 2012532688 A JP2012532688 A JP 2012532688A JP 5763652 B2 JP5763652 B2 JP 5763652B2
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- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/005—Pretreatment specially adapted for magnetic separation
- B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
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- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
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- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/005—Pretreatment specially adapted for magnetic separation
- B03C1/015—Pretreatment specially adapted for magnetic separation by chemical treatment imparting magnetic properties to the material to be separated, e.g. roasting, reduction, oxidation
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- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/025—High gradient magnetic separators
- B03C1/031—Component parts; Auxiliary operations
- B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
- B03C1/0332—Component parts; Auxiliary operations characterised by the magnetic circuit using permanent magnets
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- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/025—High gradient magnetic separators
- B03C1/031—Component parts; Auxiliary operations
- B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
- B03C1/0335—Component parts; Auxiliary operations characterised by the magnetic circuit using coils
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- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/28—Magnetic plugs and dipsticks
- B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/18—Magnetic separation whereby the particles are suspended in a liquid
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
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Description
US3951785A号は、非磁性粒子を強磁性流体に導入することにより、様々な密度の非磁性粒子を分離する方法に関する。この強磁性流体の効果的な密度は、その強磁性流体を適切な強さの磁場におくことにより調節することが可能である。
US3796660A号は、多相の水対水の混合物を、強磁性流体をその混合物に加えることにより分離することを開示しており、その強磁性流体は、主としてその相のうちの一相に溶解する。その後、この相は、磁場によって、他の相から分離することが可能である。
US3926789A号及びUS4765486A号は、微粒子混合物を、強磁性流体をその微粒子混合物に加えることにより分離することを示しており、その強磁性流体は、或る微粒子コンポーネントの表面を選択的にコートする。その後、前記コンポーネントは、残留物から磁場によって分離することができる。
WO2007/094739A1は、磁性粒子を含む液滴が外相に取り囲まれる手法を開示している。その液滴は、磁気的に表面に引きつけられ、そこでは処理がその液滴の内容物により実行される。
-磁性粒子;及び
-サンプルと不混和で、サンプルと異なる質量密度を有する液体キャリア。
多くの分子診断分析において、DNAは、それを検出できる前に、サンプルから集められ、増幅される必要がある。増幅は、PCR反応法RCAやNASBAとして知られている方法により通常生じる。これらの処理においては、その目的DNAが存在するマトリックス(matrix)は、意図した反応を抑制してはならないし、または他の方法で変質させてはならない。
Claims (25)
- 液体サンプルから磁性粒子を抽出する方法であって:
a)前記液体サンプルを液体キャリアに近接して配置するステップであり、前記液体キャリアは、前記液体サンプルと不混和であり、前記液体サンプルとは異なる質量密度を有し、当該配置によって、前記液体キャリアは、重力の影響によって前記液体サンプルの下で安定する、ステップ;及び
b)前記磁性粒子を前記液体サンプルから前記液体キャリアへ移動させる磁場を生成するステップ;
と有する、方法。 - 前記磁性粒子は前記液体キャリアから他の物質へさらに移動される、請求項1に記載の方法。
- 前記液体キャリア内の前記磁性粒子は、移動デバイスによって吸引される、請求項1に記載の方法。
- 前記液体サンプルはステップb)の後に除去される、請求項1に記載の方法。
- 液体サンプルから磁性粒子を抽出する装置であって:
前記液体サンプルが供給されるサンプルチャンバー;及び
前記液体サンプルと不混和であり、前記液体サンプルとは異なる質量密度を有する液体キャリアが供給されるキャリアチャンバー;
前記磁性粒子を前記サンプルチャンバーから前記キャリアチャンバーへ移動させることのできる、磁場を発生する磁場発生器;
を有し、
前記キャリアチャンバーは、入口境界で前記サンプルチャンバーに対して開いており、その結果、前記液体キャリアは、重力の影響によって、前記液体サンプルの下で安定し、前記配置では前記液体サンプルと前記液体キャリアが前記入口境界で接触している、装置。 - 前記キャリアチャンバーには前記液体キャリアが予め満たされている、請求項5に記載の装置。
- 前記キャリアチャンバーは、出力境界で第3チャンバーに開いている、請求項5に記載の装置。
- 前記サンプルチャンバーの表面は、少なくとも部分的に前記液体キャリアに反発し、及び/又は、前記キャリアチャンバーの表面は、少なくとも部分的に前記液体サンプルに反発する、請求項5に記載の装置。
- 前記磁性粒子の前記液体サンプルから前記液体キャリアへの移動の少なくとも1つの成分は、重力の方向と平行である、請求項5に記載の装置。
- 前記磁性粒子は前記磁場により前記液体キャリアを介して目的ゾーンに移動可能である、請求項5に記載の装置。
- 液体サンプルを処理するための物質を備えたキットであって:
磁性粒子;及び
前記液体サンプルと不混和であり、前記液体サンプルとは異なる質量密度を有する液体キャリア、
を有し、
当該キットが使用されるチャンバ内で、前記液体キャリアは、重力の影響によって、前記液体サンプルの下で安定する、
キット。 - 前記液体キャリアは前記液体サンプルよりも高い質量密度を有する、請求項11に記載のキット。
- 前記液体キャリアは、ハロゲン化油、シリコン油、フルオロシリコン油、炭化水素、及びアルカンからなるグループから選択された少なくとも1つのコンポーネントを有する、請求項11に記載のキット。
- 前記磁性粒子は前記液体キャリアに対して非湿潤である、請求項11に記載のキット。
- 前記磁性粒子は前記液体サンプルのコンポーネントを結合する、請求項11に記載のキット。
- 前記磁性粒子の前記液体サンプルから前記液体キャリアへの移動の少なくとも1つの成分は、重力の方向と平行である、請求項1に記載の方法。
- 前記磁性粒子は前記磁場により前記液体キャリアを介して目的ゾーンに移動可能である、請求項1に記載の方法。
- 前記液体キャリアは前記液体サンプルよりも高い質量密度を有する、請求項1に記載の方法。
- 前記液体キャリアは、ハロゲン化油、シリコン油、フルオロシリコン油、炭化水素、及びアルカンからなるグループから選択された少なくとも1つのコンポーネントを有する、請求項1に記載の方法。
- 前記磁性粒子は前記液体キャリアに対して非湿潤である、請求項1に記載の方法。
- 前記磁性粒子は前記液体サンプルのコンポーネントを結合する、請求項1に記載の方法。
- 前記液体キャリアは前記液体サンプルよりも高い質量密度を有する、請求項5に記載の装置。
- 前記液体キャリアは、ハロゲン化油、シリコン油、フルオロシリコン油、炭化水素、及びアルカンからなるグループから選択された少なくとも1つのコンポーネントを有する、請求項5に記載の装置。
- 前記磁性粒子は前記液体キャリアに対して非湿潤である、請求項5に記載の装置。
- 前記磁性粒子は前記液体サンプルのコンポーネントを結合する、請求項5に記載の装置。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP09172326 | 2009-10-06 | ||
EP09172326.2 | 2009-10-06 | ||
PCT/IB2010/054330 WO2011042828A1 (en) | 2009-10-06 | 2010-09-27 | Magnetic sample purification |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2013506552A JP2013506552A (ja) | 2013-02-28 |
JP5763652B2 true JP5763652B2 (ja) | 2015-08-12 |
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ID=43086060
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2012532688A Active JP5763652B2 (ja) | 2009-10-06 | 2010-09-27 | 磁性サンプルの精製 |
Country Status (6)
Country | Link |
---|---|
US (1) | US9387484B2 (ja) |
EP (1) | EP2485844B1 (ja) |
JP (1) | JP5763652B2 (ja) |
KR (1) | KR20120093258A (ja) |
CN (1) | CN102574127B (ja) |
WO (1) | WO2011042828A1 (ja) |
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BR112014003834A2 (pt) | 2011-08-22 | 2017-03-14 | Koninklijke Philips Nv | sistema e método de administração de dados, processador para uso em um sistema de administração de dados e programa de computador |
CN104395751B (zh) * | 2012-06-29 | 2016-10-19 | 皇家飞利浦有限公司 | 含有干扰颗粒的流体的处理 |
TWI555850B (zh) * | 2012-08-09 | 2016-11-01 | 財團法人工業技術研究院 | 用於分離核酸的組合物及方法 |
CN103571824B (zh) * | 2012-08-09 | 2016-04-13 | 财团法人工业技术研究院 | 用于分离核酸的组合物及方法 |
US10533170B2 (en) * | 2014-03-14 | 2020-01-14 | Shimadzu Corporation | Method for manipulating magnetic particles and device for manipulating magnetic particles |
JP6350654B2 (ja) * | 2014-05-23 | 2018-07-04 | 株式会社島津製作所 | 磁性体粒子の操作方法 |
CN106461656B (zh) | 2014-06-25 | 2020-03-24 | 皇家飞利浦有限公司 | 用于检测样品中的目标成分的生物传感器 |
MX2017002359A (es) | 2014-12-02 | 2017-05-17 | Koninklijke Philips Nv | Dispersion y acumulacion de particulas magneticas en un sistema de microfluido. |
EP3411710A1 (en) * | 2016-02-05 | 2018-12-12 | The Broad Institute Inc. | Multi-stage, multiplexed target isolation and processing from heterogeneous populations |
US11732293B2 (en) | 2017-10-06 | 2023-08-22 | The Board Of Trustees Of The University Of Illinois | Biomarker detection from fluid samples |
US20210299916A1 (en) * | 2018-08-09 | 2021-09-30 | Hummingbird Nano, Inc. | Microfluidic device and method of manufacture |
CN110873657B (zh) * | 2018-08-31 | 2022-02-08 | 北京致雨生物科技有限公司 | 一种微量样本的取样方法 |
CN111208192B (zh) * | 2020-01-15 | 2021-09-14 | 中国科学院生态环境研究中心 | 环境中磁性Fe3O4纳米颗粒分离、提取的循环富集装置及其检测方法 |
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US3951785A (en) * | 1975-01-29 | 1976-04-20 | Avco Corporation | Classification by ferrofluid density separation |
US4765486A (en) * | 1983-06-21 | 1988-08-23 | The United States Of America As Represented By The Secretary Of Agriculture | Method for obtaining a purified fraction from a mixture using a magnetic fluid |
EP0344276B1 (en) | 1987-12-01 | 1993-06-23 | Boehringer Mannheim Corporation | Methods and devices for conducting assays |
JPH02141667A (ja) * | 1988-11-22 | 1990-05-31 | Olympus Optical Co Ltd | 血球粒子 |
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EP1711263A2 (en) | 2003-12-10 | 2006-10-18 | Northeastern University | Method for efficient transport of small liquid volumes to, from or within microfluidic devices |
DE102004021780B4 (de) * | 2004-04-30 | 2008-10-02 | Siemens Ag | Verfahren und Anordnung zur DNA-Isolierung mit Trockenreagenzien |
EP1621890A1 (en) * | 2004-07-26 | 2006-02-01 | bioMerieux B.V. | Device and method for separating, mixing and concentrating magnetic particles with a fluid and use thereof in purification methods |
DE102005039175A1 (de) * | 2005-08-18 | 2007-02-22 | Qiagen Gmbh | Vorrichtung und Verfahren zum Abtrennen von magnetischen Partikeln aus einer Flüssigkeit |
BRPI0621413A2 (pt) | 2006-02-13 | 2011-12-06 | Agency Science Tech & Res | método de processamento de uma amostra biológica e/ou quìmica |
US7439014B2 (en) | 2006-04-18 | 2008-10-21 | Advanced Liquid Logic, Inc. | Droplet-based surface modification and washing |
CA2680532C (en) * | 2006-04-18 | 2017-03-21 | Advanced Liquid Logic, Inc. | Droplet-based pyrosequencing |
WO2007123908A2 (en) | 2006-04-18 | 2007-11-01 | Advanced Liquid Logic, Inc. | Droplet-based multiwell operations |
JP5390540B2 (ja) * | 2008-02-29 | 2014-01-15 | ノースウエスタン ユニバーシティ | 生物反応促進用バリア |
US9180453B2 (en) * | 2008-08-15 | 2015-11-10 | University Of Washington | Method and apparatus for the discretization and manipulation of sample volumes |
-
2010
- 2010-09-27 CN CN201080045544.XA patent/CN102574127B/zh active Active
- 2010-09-27 KR KR1020127011505A patent/KR20120093258A/ko not_active Application Discontinuation
- 2010-09-27 JP JP2012532688A patent/JP5763652B2/ja active Active
- 2010-09-27 WO PCT/IB2010/054330 patent/WO2011042828A1/en active Application Filing
- 2010-09-27 EP EP10763034.5A patent/EP2485844B1/en active Active
- 2010-09-27 US US13/500,446 patent/US9387484B2/en active Active
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EP2485844B1 (en) | 2020-07-22 |
WO2011042828A1 (en) | 2011-04-14 |
CN102574127B (zh) | 2015-11-25 |
CN102574127A (zh) | 2012-07-11 |
US20120270332A1 (en) | 2012-10-25 |
US9387484B2 (en) | 2016-07-12 |
EP2485844A1 (en) | 2012-08-15 |
JP2013506552A (ja) | 2013-02-28 |
KR20120093258A (ko) | 2012-08-22 |
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