JP5744409B2 - 人工皮膚 - Google Patents
人工皮膚 Download PDFInfo
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- JP5744409B2 JP5744409B2 JP2010048159A JP2010048159A JP5744409B2 JP 5744409 B2 JP5744409 B2 JP 5744409B2 JP 2010048159 A JP2010048159 A JP 2010048159A JP 2010048159 A JP2010048159 A JP 2010048159A JP 5744409 B2 JP5744409 B2 JP 5744409B2
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
- A61K31/4406—Non condensed pyridines; Hydrogenated derivatives thereof only substituted in position 3, e.g. zimeldine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/10—Hair or skin implants
- A61F2/105—Skin implants, e.g. artificial skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
- A61K31/4164—1,3-Diazoles
- A61K31/4184—1,3-Diazoles condensed with carbocyclic rings, e.g. benzimidazoles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/005—Enzyme inhibitors
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/07—Tetrapeptides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
- A61K8/4926—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having six membered rings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
- A61K8/4946—Imidazoles or their condensed derivatives, e.g. benzimidazoles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/362—Skin, e.g. dermal papillae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3813—Epithelial cells, e.g. keratinocytes, urothelial cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Transplantation (AREA)
- Dermatology (AREA)
- Oral & Maxillofacial Surgery (AREA)
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- Urology & Nephrology (AREA)
- Pharmacology & Pharmacy (AREA)
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- Birds (AREA)
- Vascular Medicine (AREA)
- Molecular Biology (AREA)
- Heart & Thoracic Surgery (AREA)
- Cardiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Materials For Medical Uses (AREA)
- Prostheses (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
[1] ヒト表皮由来のケラチノサイト及びヒト真皮由来の線維芽細胞を含んで成る人工皮膚形成培地中にマトリックスメタロプロテアーゼ阻害剤とヘパラナーゼ阻害剤とを添加し、該人工皮膚形成培地中の細胞を培養し、そして人工皮膚を形成させることを含む、人工皮膚の製造方法。
[2] 連続したラミナデンサ及び該ラミナデンサから生じるアンカリング線維を含む表皮基底膜とコラーゲン線維を含む真皮とを含んで成る人工皮膚であって、上記表皮基底膜中に存在する連続したラミナデンサから生じるアンカリング線維と真皮中に存在するコラーゲン線維とが結合することにより、表皮基底膜と真皮が強固に接着されていることを特徴とする、人工皮膚。
[3] 前記真皮がフィブリポジトー(fibripositor)を更に含んで成る、上記[2]に記載の人工皮膚。
[4] 前記真皮においてエラスチン線維が形成されることを特徴とする、上記[2]又は[3]に記載の人工皮膚。
[5] 上記[1]に記載の方法により製造されることを特徴とする、上記[2]〜[4]のいずれかに記載の人工皮膚。
[6] マトリックスメタロプロテアーゼ阻害剤とヘパラナーゼ阻害剤とを含んで成る培地中で形成される人工細胞であって、該人工細胞が連続したラミナデンサ及び該ラミナデンサから生じるアンカリング線維を含む表皮基底膜とコラーゲン線維を含む真皮とを含んで成り、上記表皮基底膜中に存在する連続したラミナデンサから生じるアンカリング線維と真皮中に存在するコラーゲン線維とが結合することにより、表皮基底膜と真皮が強固に接着されていることを特徴とする、人工皮膚。
本発明において使用するマトリックスメタロプロテアーゼ阻害剤としては、マトリックスメタロプロテアーゼに対して阻害活性を有する物質であればよく、特に制限はない。マトリックスメタロプロテアーゼとしては、例えばゼラチナーゼ、コラゲナーゼ、ストロメライシン、マトリライシン等が挙げられる。従って、マトリックスメタロプロテアーゼ阻害剤は、例えばゼラチナーゼ、コラゲナーゼ、ストロメライシン、マトリライシン等を阻害する物質として選択することができる。
本発明において使用するヘパラナーゼ阻害剤としては、ヘパラナーゼに対して阻害活性を有する物質であればよく、特に制限はない。ヘパラナーゼは種々の細胞に存在し、様々なヘパラン硫酸プロテオグリカンのヘパラン硫酸鎖を特異的に分解する酵素である。皮膚では、表皮を構成する表皮角化細胞及び真皮の線維芽細胞、血管内皮細胞などが産生する。
本発明における人工皮膚の製造に用いる基礎培地としては、人工皮膚の製造に従来から使用されている任意の培地を用いることができ、これらの培地としては10%の牛胎児血清を含むダルベッコ改変イーグル培地(DMEM);10%の牛胎児血清、トランスフェリン5μg/ml、インシュリン5μg/ml、tri−ヨードチロニン2nM、コレラトキシン0.1nM、ヒドロコーチゾン0.4μg/mlを含むDMEM−Ham’sF12(3:1);ケラチノサイト増殖培地(KGM)と10%牛胎児血清を含むDMEMとを1:1に混合した培地、等が挙げられる。これらの基礎培地に添加されるマトリックスメタロプロテアーゼ阻害剤の量は、その種類により異なるが、典型的には約10μg/L〜10g/Lであり、好適には約100μg/L〜1g/Lであり、そして最適には約1mg/L〜100mg/Lである。また基礎培地に添加されるヘパラナーゼ阻害剤の量は、その種類により異なるが、典型的には約10μg/L〜100g/Lであり、好適には約100μg/L〜10g/Lであり、そして最適には約1mg/L〜1g/Lである。
皮膚モデル(EFT-400、MatTeK 社製)を専用培地(EFT-400-ASY、MatTeK 社製)中で培養した。コントロール群として、ジメチルスルホキシド(DMSO)とエタノールを終濃度が0.1%となるように専用培地に添加し、ヘパラナーゼ阻害剤群として、終濃度が50μMとなるように50mMの1−[4−(1H−ベンゾイミダゾール−2−イル)−フェニル]−3−[4−(1H−ベンゾイミダゾール−2−イル)−フェニル]−ウレア(DMSO溶媒)を専用培地に添加し、MMP阻害剤群として、終濃度が10μMとなるように10mMのN−ヒドロキシ−2−[[(4−メトキシフェニル)スルホニル]3−ピコリル)アミノ]−3−メチルブタンアミド塩酸塩(CGS27023A, エタノール溶媒)を専用培地に添加し、そしてヘパラナーゼ阻害剤+MMP阻害剤群として、各々終濃度が50μM及び10μMとなるように50mMの1−[4−(1H−ベンゾイミダゾール−2−イル)−フェニル]−3−[4−(1H−ベンゾイミダゾール−2−イル)−フェニル]−ウレア(DMSO溶媒)及び10mMのCGS27023A(エタノール溶媒)を専用培地に添加して、これらを培養した。
半分にカットした培養後の上記皮膚モデルをザンボーニ固定液にて固定し、オスミウム酸にて後固定後、定法に従って樹脂に包埋した。組織の観察は超薄切片を作成し、酢酸ウラニウムによって染色したのち電子顕微鏡(日本電子株式会社製 JEM-1230)を用いて行った。通常の培養液に溶媒を添加して培養した皮膚モデル(コントロール群)、MMP阻害剤としてN−ヒドロキシ−2−[[(4−メトキシフェニル)スルホニル]3−ピコリル)アミノ]−3−メチルブタンアミド塩酸塩(CGS27023A)のみを培養液に添加し培養した皮膚モデル(A群)、ヘパラナーゼ阻害剤として1−[4−(1H−ベンゾイミダゾール−2−イル)−フェニル]−3−[4−(1H−ベンゾイミダゾール−2−イル)−フェニル]−ウレアのみを培養液に添加し培養した皮膚モデル(B群)、及びN−ヒドロキシ−2−[[(4−メトキシフェニル)スルホニル]3−ピコリル)アミノ]−3−メチルブタンアミド塩酸塩(CGS27023A)と1−[4−(1H−ベンゾイミダゾール−2−イル)−フェニル]−3−[4−(1H−ベンゾイミダゾール−2−イル)−フェニル]−ウレアの両方を培養液に添加し培養した皮膚モデル(C群)の比較を微細形態学的に行った。その結果を以下の表に示す。
Claims (3)
- ヒト表皮由来のケラチノサイト及びヒト真皮由来の線維芽細胞を含んで成る人工皮膚形成培地中にN−ヒドロキシ−2−[[(4−メトキシフェニル)スルホニル]3−ピコリル)アミノ]−3−メチルブタンアミド塩酸塩と1−[4−(1H−ベンゾイミダゾール−2−イル)−フェニル]−3−[4−(1H−ベンゾイミダゾール−2−イル)−フェニル]−ウレアとを添加し、該人工皮膚形成培地中の細胞を培養し、そして人工皮膚を形成させることを含む、人工皮膚の製造方法。
- 連続したラミナデンサ及び該ラミナデンサから生じるアンカリング線維を含む表皮基底膜とコラーゲン線維を含む真皮とを含んで成る人工皮膚であって、上記表皮基底膜中に存在する連続したラミナデンサから生じるアンカリング線維と真皮中に存在するコラーゲン線維とが結合することにより、表皮基底膜と真皮が強固に接着されており、前記真皮がフィブリポジトー(fibripositor)を更に含んで成り、そして、前記真皮においてエラスチン線維が形成されていることを特徴とする、人工皮膚。
- 請求項1に記載の方法により製造されることを特徴とする、請求項2に記載の人工皮膚。
Priority Applications (13)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2010048159A JP5744409B2 (ja) | 2010-03-04 | 2010-03-04 | 人工皮膚 |
PCT/JP2011/050887 WO2011108300A1 (ja) | 2010-03-04 | 2011-01-19 | 人工皮膚 |
EP11750413.4A EP2543396B1 (en) | 2010-03-04 | 2011-01-19 | Artificial skin |
CN201180012330.7A CN102781484B (zh) | 2010-03-04 | 2011-01-19 | 人工皮肤及其制造方法 |
US13/582,199 US8871505B2 (en) | 2010-03-04 | 2011-01-19 | Artificial skin |
RU2012142198/15A RU2594118C2 (ru) | 2010-03-04 | 2011-01-19 | Искусственная кожа |
BR112012022238A BR112012022238A2 (pt) | 2010-03-04 | 2011-01-19 | pele artificial |
AU2011222243A AU2011222243B2 (en) | 2010-03-04 | 2011-01-19 | Artificial skin |
KR1020127022359A KR101758410B1 (ko) | 2010-03-04 | 2011-01-19 | 인공 피부 |
KR1020177018742A KR101846138B1 (ko) | 2010-03-04 | 2011-01-19 | 인공 피부 |
HK13104906.0A HK1177699A1 (en) | 2010-03-04 | 2013-04-23 | Artificial skin and manufacturing method thereof |
US14/496,534 US9579310B2 (en) | 2010-03-04 | 2014-09-25 | Artificial skin |
US15/363,011 US20170071922A1 (en) | 2010-03-04 | 2016-11-29 | Artificial skin |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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JP2010048159A JP5744409B2 (ja) | 2010-03-04 | 2010-03-04 | 人工皮膚 |
Publications (2)
Publication Number | Publication Date |
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JP2011182823A JP2011182823A (ja) | 2011-09-22 |
JP5744409B2 true JP5744409B2 (ja) | 2015-07-08 |
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JP2010048159A Active JP5744409B2 (ja) | 2010-03-04 | 2010-03-04 | 人工皮膚 |
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EP (1) | EP2543396B1 (ja) |
JP (1) | JP5744409B2 (ja) |
KR (2) | KR101758410B1 (ja) |
CN (1) | CN102781484B (ja) |
AU (1) | AU2011222243B2 (ja) |
BR (1) | BR112012022238A2 (ja) |
HK (1) | HK1177699A1 (ja) |
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KR20150024490A (ko) | 2013-08-26 | 2015-03-09 | 삼성전자주식회사 | 열에 의하여 치유 및 재성형이 가능한 전도성 하이드로겔 복합재료 |
US9435745B2 (en) | 2014-12-23 | 2016-09-06 | Colgate-Palmolive Company | Measuring the rate of release of metal ions |
US9625449B2 (en) | 2014-12-23 | 2017-04-18 | Colgate-Palmolive Company | Determining the bioavailability of zinc (II) ions |
US9354219B1 (en) | 2014-12-23 | 2016-05-31 | Colgate-Palmolive Company | Detecting stannous ions |
AU2016228525B2 (en) * | 2015-03-12 | 2021-01-21 | Alarm.Com Incorporated | Virtual enhancement of security monitoring |
EP3530750A4 (en) * | 2016-10-21 | 2020-08-12 | Shiseido Company, Ltd. | LAMININE 511 MANUFACTURING PROMOTER, BASAL EPIDERMAL LAYER STABILIZER, AND / OR SCREENING PROCEDURES FOR A MEANS OF MINIMIZING THE REDUCTION IN OR PROMOTING Elevation IN EPIDERMAL STEM CELLS |
KR102226056B1 (ko) | 2018-09-13 | 2021-03-11 | 한국과학기술연구원 | 생체피부와 물리적 및 생물학적으로 유사한 인공 표피층, 인공 진피층 및 이를 포함하는 인공피부 |
JP7412096B2 (ja) * | 2019-06-14 | 2024-01-12 | 株式会社 資生堂 | 皮膚様組織の製造方法、及びそれにより得られる皮膚様組織 |
CN113740491A (zh) * | 2021-08-27 | 2021-12-03 | 无限极(中国)有限公司 | 一种用于感官评价的仿真皮肤及其制备方法 |
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GB9714936D0 (en) | 1997-07-17 | 1997-09-17 | Smith & Nephew | Cell culture products |
JP4074043B2 (ja) * | 2000-03-27 | 2008-04-09 | 株式会社資生堂 | 皮膚基底膜形成促進剤、人工皮膚形成促進剤及び人工皮膚の製造方法 |
JP2004075661A (ja) | 2002-06-18 | 2004-03-11 | Shiseido Co Ltd | 表皮基底膜ケアを特徴とする皮膚外用剤、皮膚基底膜構造形成促進剤および人工皮膚の製造方法 |
CA2641612A1 (en) * | 2006-02-07 | 2007-08-16 | Organogenesis, Inc. | Bioengineered tissue constructs and cardiac uses thereof |
EP2086553A4 (en) | 2006-10-20 | 2010-12-29 | Univ Australian | INHIBITION OF EXTRACELLULAR MATRIX DEGRADATION |
JP5722030B2 (ja) | 2008-03-31 | 2015-05-20 | 株式会社 資生堂 | しわを防止または改善するための経口、注射、皮膚外用剤および美容方法 |
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- 2011-01-19 KR KR1020177018742A patent/KR101846138B1/ko active IP Right Grant
- 2011-01-19 EP EP11750413.4A patent/EP2543396B1/en active Active
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- 2011-01-19 AU AU2011222243A patent/AU2011222243B2/en not_active Ceased
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- 2011-01-19 US US13/582,199 patent/US8871505B2/en active Active
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Also Published As
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WO2011108300A1 (ja) | 2011-09-09 |
CN102781484A (zh) | 2012-11-14 |
BR112012022238A2 (pt) | 2017-08-08 |
US8871505B2 (en) | 2014-10-28 |
CN102781484B (zh) | 2015-06-03 |
JP2011182823A (ja) | 2011-09-22 |
US9579310B2 (en) | 2017-02-28 |
KR101846138B1 (ko) | 2018-04-05 |
US20150011593A1 (en) | 2015-01-08 |
EP2543396A4 (en) | 2013-08-14 |
EP2543396B1 (en) | 2019-04-10 |
RU2594118C2 (ru) | 2016-08-10 |
EP2543396A1 (en) | 2013-01-09 |
KR20130006437A (ko) | 2013-01-16 |
RU2012142198A (ru) | 2014-04-10 |
KR101758410B1 (ko) | 2017-07-14 |
AU2011222243B2 (en) | 2015-07-09 |
US20170071922A1 (en) | 2017-03-16 |
AU2011222243A1 (en) | 2012-09-20 |
KR20170083642A (ko) | 2017-07-18 |
HK1177699A1 (en) | 2013-08-30 |
US20130006355A1 (en) | 2013-01-03 |
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