JP5737896B2 - Cosmetics - Google Patents

Description

  The present invention is highly safe and exhibits excellent skin beautification and whitening effects, and is also useful as an external quasi-drug, basic cosmetic, makeup cosmetic, bath preparation, hair cosmetic, etc. About.

  Conventionally, an extract obtained from a marine flowering plant, for example, a plant of the genus Amamoaceae, has both high moisturizing ability and excellent tyrosinase activity inhibitory action, antioxidant action and ultraviolet light absorption, and the extract has excellent makeup. It was known that it was a material (Patent Document 1, Non-Patent Document 1). Furthermore, it is also known that an extract of a marine flowering plant exhibits a hair restoration / hair growth effect based on testosterone-5α-reductase inhibitory action (Patent Document 2).

Japanese Patent No. 2971549 JP 2006-306816 A Kazufumi Naito FRAGRANCE JOURNAL Vol.27, No.1 p114-118

However, when an extract is produced from a plant belonging to a marine flowering plant (for example, the genus Amamoaceae), as shown in Patent Document 1, the extract exhibits ultraviolet absorptivity, ie, ultraviolet May have an absorption maximum in the region. Thus, having an absorption maximum in the ultraviolet region has the advantage that the extract protects the skin from ultraviolet rays, while the components contained in the extract absorb and excite ultraviolet rays, This means that there is a possibility of exhibiting phototoxicity (irritation) and / or photosensitization, and in that sense, extracts obtained from plants belonging to marine flowering plants should be applied to skin cosmetics. It can be said that there is a problem to be solved.
Furthermore, in recent years, there has been a demand for cosmetics that can prevent and improve skin aging and troubles caused by various factors such as ultraviolet rays, stress, and aging more and more effectively. The creation and improvement of further effectiveness are also demanded for marine flowering plants known as materials.

  In light of the problems of the prior art, the present inventors have intensively studied and studied methods for solving and improving those problems, and as a result, extracts obtained from plants belonging to marine flowering plants, UV absorption while retaining the effectiveness of extracts that have not been treated by adsorption treatment with activated carbon or nonionic porous resin, ultrafiltration treatment, or fermentation treatment with microorganisms It is found that a treated product that does not exhibit the properties can be obtained, and that the treatment can newly create a fibroblast activation effect, and when the treated product is used as a cosmetic composition, it has excellent skin and whitening effects. And it discovered that the cosmetics excellent in biological safety could be provided, and came to complete this invention.

That is, the present invention is an adsorption treatment with activated carbon or a nonionic porous resin, ultrafiltration treatment, or fermentation treatment on an extract of a plant of the family Zosteraceae (Zostera sp.). It is a cosmetic containing a processed product obtained by applying one or more treatments.
Here, the term cosmetics is used in a broad sense including so-called cosmetics and quasi-drugs.

  According to the present invention, for an extract obtained from a plant of the genus Amamoaceae which is a marine flowering plant, any one of adsorption treatment with activated carbon or nonionic porous resin, ultrafiltration treatment and fermentation treatment Since a treatment product obtained by applying one or more treatments is blended in cosmetics, it exhibits no ultraviolet absorptivity and has excellent skin physiological activity (fibroblast activation, moisturizing, By the action of the processed product having an antioxidative action and a whitening action, it is possible to provide a cosmetic having excellent skin and whitening effects and excellent safety.

FIG. 1 is a diagram showing the measurement results of the ultraviolet maximum absorption wavelength of a treated product obtained by subjecting an extract of the genus Amamoaceae plant of Production Example 1 to activated carbon treatment. FIG. 2 is a diagram showing the measurement results of the ultraviolet maximum absorption wavelength of a treated product obtained by subjecting an extract of the genus Amamoaceae plant of Production Example 2 to activated carbon treatment. FIG. 3 is a diagram showing the measurement results of the ultraviolet maximum absorption wavelength of a treated product obtained by subjecting an extract of the genus Amamoaceae plant of Production Example 3 to a yeast fermentation treatment. FIG. 4 is a diagram showing the measurement results of the ultraviolet maximum absorption wavelength of a processed product obtained by subjecting an extract of the genus Amamoaceae plant of Production Example 4 to a yeast fermentation process. FIG. 5 is a diagram showing a measurement result of an ultraviolet maximum absorption wavelength of a treated product obtained by subjecting an extract of the genus Amamoaceae plant of Production Example 5 to a lactic acid bacteria fermentation treatment. FIG. 6 is a diagram showing the measurement results of the ultraviolet maximum absorption wavelength of a treated product obtained by subjecting an extract of the genus Amamoaceae plant of Production Example 6 to a lactic acid bacteria fermentation treatment. FIG. 7 is a diagram showing a measurement result of an ultraviolet absorption maximum wavelength of a processed product obtained by subjecting an extract of the genus Amamoaceae plant of Production Example 7 to a fermentation process of Bacillus natto. FIG. 8 is a diagram showing a measurement result of an ultraviolet absorption maximum wavelength of a processed product obtained by subjecting an extract of the genus Amamoaceae plant of Production Example 8 to a Tempe fungus fermentation treatment. FIG. 9 is a diagram showing the measurement results of the ultraviolet absorption maximum wavelength of a treated product obtained by subjecting an extract of the genus Amamoaceae plant of Production Example 9 to an adsorption treatment with a nonionic porous resin. FIG. 10 is a diagram showing the measurement results of the ultraviolet absorption maximum wavelength of a treated product obtained by subjecting an extract of the genus Amaranthaceae plant of Production Example 10 to an adsorption treatment with a nonionic porous resin. FIG. 11 is a diagram showing a measurement result of an ultraviolet maximum absorption wavelength of a treated product obtained by subjecting an extract of the genus Amamoaceae plant of Production Example 11 to an ultrafiltration treatment. FIG. 12 is a diagram showing a measurement result of an ultraviolet absorption maximum wavelength of a treated product obtained by subjecting an extract of the genus Amamoaceae plant of Production Example 12 (Comparative Production Example 1) to activated carbon treatment. FIG. 13 is a diagram showing a measurement result of an ultraviolet maximum absorption wavelength of an extract of a genus Amamoaceae plant of Comparative Production Example 1.

  The present invention prepares an extract from a plant of the genus Amamoaceae, which is a marine flowering plant, and the extract is subjected to adsorption treatment with activated carbon or a nonionic porous resin, ultrafiltration treatment, and fermentation treatment. It is a cosmetic containing a processed product obtained by applying any one or two or more of them. Hereinafter, a method for preparing an extract of a plant belonging to the genus Amamoaceae in the present invention, a preferred specific example of an adsorption treatment with activated carbon or a nonionic porous resin, an ultrafiltration treatment, or a fermentation treatment for the extract will be described. .

  Examples of the plants of the genus Amamoaceae, which are marine flowering plants used in the preparation of the present invention, include, for example, eelgrass (Zostera marina), core eel (Zostera japonica), giant eel (Zostera asiatica), sedge eel (Zostera caespitosa), and sea lion (Zostera caulescens). In the present invention, among these genus Amamo plants, Amamo (whole plant is preferred) is the most preferable from the viewpoint of easy availability of raw materials.

  The extract of the plant of the genus Amamoaceae is prepared by pre-washing the part to be extracted (for example, whole grass) as necessary, drying, preferably further chopping or pulverizing, dipping method, countercurrent extraction It can be carried out by contacting with an extraction solvent by an appropriate means such as a method. The preparation can also be performed by using a supercritical extraction method.

  As an extraction solvent, water; lower alcohols such as methanol, ethanol, and propanol; higher alcohols such as oleyl alcohol, stearyl alcohol, and octyldodecanol; various solvents such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin Monohydric alcohols; esters such as ethyl acetate, butyl acetate, methyl propionate and 2-ethylhexyl glyceride; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; n-hexane, toluene and chloroform Examples thereof include hydrocarbon solvents, which are used alone or in combination of two or more. Among these, it is preferable to use one kind of single solvent or two or more kinds of mixed solvents selected from water, lower alcohols and polyhydric alcohols from the viewpoint that they can be widely applied to cosmetics. Single use is most preferred.

  The mixing ratio in the case of using a mixed solvent is, for example, 1: 1 to 25: 1 by volume ratio (hereinafter the same) if the mixed solvent is water and ethyl alcohol, and 1: if the mixed solvent is water and glycerin. In the case of a mixed solvent of 1 to 20: 1, or water and 1,3-butylene glycol, it is preferably in the range of 1: 1 to 20: 1.

  In the preparation of the extract of the present invention, the pH of the extract is preferably maintained in the range of 4 to 8. In this sense, if necessary, the extraction solvent may be sodium hydroxide, sodium carbonate, potassium hydroxide. Alternatively, an alkaline adjusting agent such as arginine, an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid, sulfuric acid, or the like may be blended to adjust to a desired pH.

  Extraction conditions such as extraction temperature and time vary depending on the type of solvent used, the extraction method, and the like. For example, in the case of the immersion method, the extraction temperature is preferably in the range of 4 to 90 ° C., and the extraction time is 0. About 1 to 1 week is preferred

  The mixing ratio of whole dried eel to the above extraction medium is generally in the range of 1: 1 to 1: 1000 by weight, preferably 1:10 to 1: 100, more preferably 1:20 to 1:50. Range. Since the dried eel has a low bulk density, if the amount of eel is too high, contact with the liquid will be poor and the material will not be used effectively, and the liquid will be absorbed by the material and the yield will be small. There is a possibility.

  After the extraction treatment under the above extraction conditions, next, an adsorption treatment using activated carbon or a nonionic porous resin or the like for the extract from the plant of the genus Amamoaceae which is a marine flowering plant, A filtration process or a fermentation process is performed.

  As the activated carbon used for the adsorption treatment, in addition to plant materials such as pine trees, bamboo, coconut shells, walnut shells, etc., it is also possible to use coal or petroleum as raw materials. In addition, the activated carbon is treated with a physical method such as high-temperature carbonization using gas such as water vapor, carbon dioxide, air, etc., or treated with chemicals such as zinc chloride, and heated to make it porous. Alternatively, a product obtained by performing an activation treatment by a chemical method may be used. Even if any activated carbon is used, the ultraviolet absorption effect disappears, and the fibroblast activation effect is created, and further, the moisturizing effect, the antioxidant effect, and the whitening effect are maintained. From the standpoint, activated carbon obtained by activating a physical method using a plant material such as a tree such as pine, bamboo, coconut shell, and walnut shell as a raw material is preferable. Further, the activated carbon has various particle sizes from granular to fine powder, and fine powder is desirable from the adsorbing capacity.

  The amount of activated carbon added for the adsorption treatment is 0.01 to 10.0 parts by weight, preferably 0.1 to 5.0 parts by weight, more preferably 0.5 to 2 parts by weight, based on the solid content of the extract. If the amount is less than this, the ultraviolet absorptivity does not sufficiently disappear, and if it is more than this, there is a possibility that even a component showing effectiveness is removed.

  The time for mixing and stirring the activated carbon is from 1 minute to 8 hours, preferably from 10 minutes to 3 hours, more preferably from 30 minutes to 2 hours. Even if the length is longer than this, the adsorption does not proceed more than a certain amount and is not only useless, but also there is a possibility that even components showing effectiveness are removed.

  When activated carbon treatment is applied to the above extract, after adding powdered or granular activated carbon to the extract and stirring, a method of removing the activated carbon, a method of treating the extract by flowing it through an activated carbon column, or activated carbon A method of passing the extract through a filter paper or a cartridge filter is usually used, but from the viewpoint of adsorption efficiency, the process of adding activated carbon to the extract and stirring is most preferable. The raw materials for activated carbon include sawdust, pine and bamboo wood chips, charcoal, coconut shells and other plant materials, as well as coals such as grass and peat, or petroleum. In view of the above, activated carbon made of plant material is preferable. Furthermore, considering the adsorption performance, powdered activated carbon is preferable, and the particle diameter is preferably 100 mesh or less (0.15 mm). However, if it is too fine, it is difficult to remove it from the product, so 0.5 μm (0.0005 mm) or more that can be captured by a membrane filter having a pore diameter of 0.45 μm or 0.2 μm is preferable.

Moreover, it is also possible to use a nonionic porous resin as an adsorbent. As the nonionic porous resin, a resin such as a styrene / divinylbenzene copolymer or a methacrylic acid ester polymer is used, but the specific surface area is generally 100 to 2000 m ² / g, preferably 300 to 1000 m ² / g, fine. A porous resin having a pore volume generally in the range of 0.1 to 3.0 mL / g, preferably 0.5 to 1.5 mL / g is used. Examples of such nonionic porous resins include styrene / divinylbenzene-based diamond ions HP10, 20,21, Sepabeads SP800, SP850, SP700, SP207 (hereinafter Mitsubishi Chemical Corporation), Amber. Light XAD4, 16, Duolite S874, 877 (Rohm and Haas), Methacrylate-based Diaion HP1MG, 2MG (Mitsubishi Chemical Corporation), Amberlite XAD7 (Rohm and Haas) ) And the like.

  The conditions for the adsorption treatment with the nonionic porous resin may be either a batch type or a continuous type. For example, in the case of a batch type, 0.1 to 200 parts by weight, preferably 1 to 100 parts by weight, and more preferably 10 to 50 parts by weight of nonionic porous resin with respect to 1 part by weight of the solid content of the extract. In addition, it can be adsorbed at 4 to 80 ° C., preferably 20 to 40 ° C. If it is less than this, the ultraviolet absorptivity will not be sufficiently lost, and if it is more than this, there is a possibility that even a component showing effectiveness is removed.

  Further, the adsorption treatment time is 10 minutes to 24 hours, preferably 30 minutes to 3 hours. In the case of the continuous type, 50 to 500 parts by weight of a nonionic porous resin is packed in a column or the like with respect to 1 part by weight of the solid content of the extract, and the extract solution is passed through for 30 minutes to 3 hours. The adsorption process can be performed with If it is shorter than this, the UV absorption property will not disappear sufficiently, and if it is longer than this, the adsorption will not proceed to a certain extent and it will not only be useless, but it will also remove components that show effectiveness. There is sex.

  Moreover, when performing an ultrafiltration process, an ultrafiltration membrane is installed in an ultrafiltration apparatus and a filtration process is performed. About the filtration molecular size of a filtration membrane, MWCO (fraction molecular weight) 5,000-MWCO100, If the size is more than 000, the ultraviolet absorptivity does not sufficiently disappear, and if the size is smaller than this, there is a possibility that even a component showing effectiveness is removed. The filtration time varies depending on the amount of liquid to be treated, the flow rate of the liquid, the performance of the filtration membrane, etc., but the end point is the time when the liquid before the filtration treatment runs out and is collected in the treatment liquid tank.

  The treated product obtained by performing the adsorption treatment or the ultrafiltration treatment is generally adjusted to a pH of 4 to 8 and then mixed with cosmetics as it is or, if necessary, by vacuum concentration or the like. It is blended in cosmetics after adjusting the concentration. In some cases, the powder may be pulverized according to a conventional method such as spray drying or freeze drying.

  Further, even with the above-described adsorption treatment (adsorption treatment with activated carbon or nonionic porous resin) or single treatment of ultrafiltration treatment, the ultraviolet absorption of the extract is sufficiently lost and the fibroblast activation effect is obtained. Although it can be created, if necessary, the adsorption treatment and the ultrafiltration treatment may be combined.

  Next, the preferable specific example in the case of performing a fermenting process with respect to the extract of the plant which belongs to the genus Amamoaceae is demonstrated. Examples of the bacteria used in the fermentation treatment include yeast, lactic acid bacteria, natto bacteria, and tempe bacteria. Even when any of these bacteria is used, the ultraviolet absorptivity disappears and fibroblast activation is achieved. Although an effect is created and a moisturizing effect, an antioxidant effect, and a whitening effect are maintained, yeast or lactic acid bacteria are more preferable in terms of the strength of the effect.

Examples of yeast used for the fermentation of eelgrass extract include Saccharomyces (Saccharomyces).
cerevisiae) (including those from marine origin), Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carlsbergensis, Saccharomyces bayonas (Saccharomyces
bayonus) and other yeasts of the genus Saccharomyces; Torulaspora delbruekii, Torulaspora fermentati, Torulaspora rosei (Torulaspora)
rosei) and other yeasts; Zygosaccharomyces
rouxii), Zygosaccharomyces soya, Zygosaccharomyces sake, Zygosaccharomyces sake, Zygosaccharomyces sake
miso), yeasts of the genus Gigosaccharomyces such as Zygosaccharomyces lactis; Candida versatilis, Candida etchellsii, Candida kefyr, Candida sake Saccharomyces cerevisiae is most preferred because it can be used most widely in foods and has a strong fermenting power. Candida scottii can be used.

Examples of lactic acid bacteria used for the fermentation of eelgrass extracts include lactic acid bacteria originating from the ocean such as Marinilactobacillus phychrotolerans; Lactobacillus plantarum, Lactobacillus brevis (L .
brevis), Lactobacillus lactic acid bacteria such as L. casei; Carnobacterium divergence (Carmobacterium)
divergens), lactic acid bacteria of the genus Carmobacterium such as Carmobacterium piscicola; Leuconostoc
mesenteroides), lactic acid bacteria of the genus Leuconostoc such as Leuconostoc citreum; Streptococcus
faecalis), Streptococcus pyogenes and other Streptococcus lactic acid bacteria; Enterococcus caseliflavus, Enterococcus
lactic acid bacteria of the genus Enterococcus such as sulfreus; Lactococcus plantarum, Lactococcus raffinolactis
Lactic acid bacteria of the genus Lactococcus such as rafinolactis; Lactic acid bacteria of the genus Weissella such as Weissella confusa and Weissella kandleri; Atopobium minutum and Atopobium parvulus Lactic acid bacteria of the genus Atopobium; Vagococcus
fluvialis), lactic acid bacteria of the genus Vagococcus, such as Vagococcus salmoninarum; Pediococcus damnosus, Pediococcus pentosedus, etc. Among these lactic acid bacteria, use of Marinilactobacillus phychrotolerans is most preferable from the viewpoint of the effect of the fermented product obtained and ease of handling.

In addition, Bacillus natto, Bacillus subtilis and other natto bacteria, Rhizopus microsporus oligosporus, Rhizopus oryzae (Rhizopus)
oryzae) and the like can also be used in the same manner.

  As a medium used for preparing an extract of a plant belonging to the genus Amamoaceae in the fermentation treatment, water, a mixture of water and lower alcohols such as ethanol and propanol, water and ethylene glycol, propylene glycol , 1,3-butylene glycol and other mixed liquids, water and sorbitol, saccharides such as glucose and the like can be used, but the fungus used for fermentation is most effective, The use of water alone, which has few factors affecting the growth of the fungus, is most preferred in that it does not contain any component that becomes a nutrient source of the fungus in addition to the contained component.

  When performing the fermentation treatment, the extract is sterilized. As a sterilization method, an autoclave sterilization method in which the extract is heated at 120 to 130 ° C. for 10 to 20 minutes, or 80 to 90 ° C. for 60 to 120 minutes. A heat sterilization method such as an intermittent sterilization method in which the holding is repeated once a day for 2 to 3 days is generally used. Further, sterilization treatment by membrane filter filtration is also possible.

After performing the sterilization treatment as described above, the sterilized liquid is placed in a fermentation tank, and microorganisms are inoculated therein to perform fermentation. The appropriate amount of inoculated microorganism is 10 ⁶ to 10 ⁸ cells / mL. Even if the inoculation amount is larger than the above range, the progress time of fermentation hardly changes. On the other hand, if the inoculation amount is smaller than the above range, it takes a long time to complete the fermentation, which is not preferable.

  If fermentation temperature is the range of 5-50 degreeC, fermentation will advance and the target fermented material can be obtained, More preferably, it is the range of 25-40 degreeC which is the optimal growth temperature of each microbe. The number of fermentation days is generally 1 to 10 days, more preferably 2 to 5 days in the case of performing fermentation at the optimum temperature. When the fermentation days are shorter than one day, the fermentation is not sufficiently performed, and it becomes difficult to obtain a target fermented product having no UV absorption. On the other hand, if the number of days of fermentation exceeds 10 days, it is not preferable because not only the improvement of the ultraviolet absorption and removal action is not observed, but also inconveniences such as an increase in coloring and fermentation odor.

  If the predetermined fermentation days have elapsed, the fermentation liquid is sterilized using, for example, a method of heating at 80 to 90 ° C. for 60 to 120 minutes, and the fermentation is stopped, followed by solid-liquid separation means such as filtration or centrifugation. Is used to remove insoluble matter to obtain the desired fermentation broth. The fermented liquor obtained here is generally adjusted to a pH of 4 to 8 and then blended in the cosmetic as it is, or if necessary, blended in the cosmetic after being adjusted to a predetermined concentration by vacuum concentration or the like. . In some cases, the powder may be pulverized according to a conventional method such as spray drying or freeze drying. In addition, although the extract has already been in a state where ultraviolet absorption is not recognized by performing the fermentation treatment, adsorption with activated carbon or nonionic porous resin is performed on the fermentation broth for the purpose of adjusting the color tone. Processing may be performed.

  It should be noted that an enzyme hydrolysis treatment is performed at the time of preparing the extract as a pre-stage for performing the adsorption treatment with the activated carbon or the nonionic porous resin or the ultrafiltration treatment, or in parallel with the extraction treatment. Moreover, when performing a fermentation process, you may perform the hydrolysis process by an enzyme before fermentation or in parallel with fermentation. This makes it possible to more effectively use plant components belonging to the genus Amamo.

  When performing an enzymatic hydrolysis treatment, as the enzyme, at least one enzyme selected from saccharide-degrading enzymes or proteolytic enzymes is used, or at least one selected from saccharide-degrading enzymes or proteolytic enzymes is used. It is preferable to use in combination.

  Examples of carbohydrases include α-amylase, β-amylase, glucoamylase, cellulase, hemicellulase, β-glucanase, β-xylanase, dextranase, polygalacturonase, α-galactosidase, β-galactosidase, pullulanase. , Isoamylase, α-glucosidase, β-glucosidase, maltotriohydrolase, pectinase, pectin depolymerase, pectin demethoxylase, pectin lyase, pectin esterase and the like can be used. Among these enzymes, α-amylase, pectinase and cellulase are particularly preferable.

Examples of proteolytic enzymes include actinases such as actinase, pepsins such as pepsin, trypsins such as trypsin and chymotrypsin, papains such as papain and chymopapain, peptidases such as glycylglycine peptidase, carboxypeptidase and aminopeptidase. And bromelain can be used.
Among these enzymes, actinases such as actinase and papains such as papain and chymopapain are particularly preferable.

  The amount of enzyme used should be in the range of 0.01 to 5.0% by weight, more preferably 0.05 to 2.0%, based on the weight of the dried eel at the time of extraction. It is in the range of wt%. At the time of fermentation, 0.1 to 2.0% by weight of the solid content weight (converted from the amount of evaporation residue) of the liquid to be fermented is preferable.

  The treatment conditions such as pH, temperature, and time in the enzymatic decomposition treatment are preferably performed for 1 to 24 hours near the optimum pH and temperature of the enzyme used.

  Examples of cosmetics comprising an adsorbed treated product of activated carbon or nonionic porous resin of the plant belonging to the genus Amamoaceae of the present invention, a ultrafiltered product, or a fermented product include, for example, emulsions, creams, lotions, Makeup cosmetics such as essences, packs, facial cleansers, lipsticks, foundations, liquid foundations, makeup press powders, hair shampoos, hair rinses, hair treatments, conditioners, hair dyes, hair conditioners, etc. And cosmetics, facial cleansers, body shampoos, soaps and other cleaning cosmetics, and bath agents, but of course are not limited thereto.

  The blended amount of the processed product in the cosmetic of the present invention is generally 0.001 to 5.0% by weight, preferably 0.01 to 2.0% by weight in the case of a basic cosmetic as a solid content. The range is generally 0.001 to 3.0% by weight for makeup cosmetics, preferably 0.01 to 1.0% by weight, and is generally 0.001 to 5. In the range of 0% by weight, preferably 0.01 to 2.0% by weight, and in the case of a bath agent, generally in the range of 0.001 to 5.0% by weight, preferably 0.01 to 2.0% by weight. is there.

  In addition to the above-mentioned essential ingredients, the cosmetics of the present invention include compounding ingredients usually used in cosmetics, such as oily ingredients, surfactants, moisturizers, thickeners, antiseptic / disinfectants, powder ingredients, ultraviolet rays. Absorbers, pigments, fragrances, antioxidants, physiologically active ingredients and the like can be appropriately blended as necessary.

  Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Oils derived from plants such as macadamia nut oil and olive squalane; Fats derived from animals such as squalane, mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax and lanolin; liquid paraffin, petrolatum, paraffin wax and squalane Hydrocarbons such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid, etc .; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Acid isop Pills, butyl oleate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, Quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.
Also, as emulsifiers or emulsifiers, stevia derivatives such as enzyme-treated stevia, lecithin and derivatives thereof, lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, beans, millet, etc.), extract of Zizyphus joazeiro A thing etc. can also be mix | blended.

  Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, mucopolysaccharides (for example, hyaluron). Acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof, elastin and derivatives thereof, collagen and derivatives thereof, hydrolyzed silk protein, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, phytosterol, soybean phospholipid , Cholesteryl isostearate, seaweed extract, seafood-derived collagen and its derivatives, various amino acids and their derivatives (such as trimethylglycine), beech extract, soy milk Liquid, lotus seed fermentation broth, pearl barley fermentation liquor, royal jelly fermentation liquor, rice-derived extract and fermented, and the like.

  Examples of thickeners include, for example, brown algae such as alginic acid, agar, carrageenan, fucoidan, green algae or red algae-derived components, beech extract, pectin, locust bean gum, polysaccharides such as aloe polysaccharide, xanthan gum, tragacanth gum, guar gum, etc. Synthetic polymers such as gums, cellulose derivatives such as carboxymethylcellulose, hydroxyethylcellulose, polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives, polyglutamic acid and its derivatives, Examples thereof include sugar compounds mainly composed of glucosyl trehalose and hydrolyzed hydrogenated starch.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Examples include luconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), 1,2-pentanediol, various essential oils, dry bark, propolis extract, and methylisothiazolinone.

  Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, 6- or 12-nylon powder, polyethylene powder. Silk powder, cellulosic powder, grains (rice, wheat, corn, millet, etc.) powder, beans (soybean, red beans, etc.) powder, and the like.

  Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives, ubidecaquinone (ubiquinone), rutin, rutin glucoside, white coconut extract, rice extract, murasakikibu extract, birch extract Products, hamamelis extract, oolong tea extract, black bean hydrolyzed extract, hagoromogusa extract and the like.

Furthermore, if necessary, other physiologically active ingredients (whitening agent, skin aging prevention / roughness improving agent, etc.) may be blended within the range that does not impair the effects and features of the fermented product used in the present invention. For example, if it is a whitening agent, t-cycloamino acid derivative, kojic acid and its derivative, ascorbic acid and its derivative, hydroquinone derivative, ellagic acid and its derivative, resorcinol derivative, placenta extract, suhakuhi extract, yukinoshita extract , Rice bran extract, rice bran extract hydrolyzate, lactic acid bacteria fermented rice, lactic acid bacteria fermented germinated rice, lactic acid bacteria fermented cereals (barley, beans, millet), white coconut extract, white coconut hydrolyzed extract, murasakixikib extract, Lotus seed fermented product, ginseng extract, Pandanus amaryllifolius Roxb. Extract, Arcangelisia flava (Arc angelicia flava Merrilli) extract, lizard carrot (Codonopsis)
pilosula) extract, chamomile extract (trade name: chamomile ET), ginkgo extract, clam squirrel extract, itadori extract, squirrel foliage extract, licorice extract, licorice poppo extract, altea extract, geno shoko extract, yukinoshita extract Product, jujube extract, peony extract, toki extract, peach extract, seaweed extract such as kombu, linoleic acid and its derivatives or processed products (such as liposomal linoleic acid), 2,5-dihydroxybenzoic acid If the derivative or the like is a component for preventing skin aging and improving rough skin, animal or fish-derived collagen and derivatives thereof, elastin and derivatives thereof, intercellular lipids such as ceramide, nicotinic acid and derivatives thereof, glycyrrhizic acid and derivatives thereof ( Dipotassium salts, etc.), t-cycloamino acid derivatives, vitamin A and derivatives thereof , Vitamin E and its derivatives (such as sodium d, l-α-tocopheryl phosphate), allantoin, α-hydroxy acids, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, coenzyme Q-10, α-lipo Herbal extract such as acid, ergothioneine, gentian extract, licorice extract, pearl barley extract, chamomile extract, carrot extract, aloe extract, rice bran extract hydrolyzate, rice extract hydrolysate, low allergen rice extract hydrolysate, Rice Ferment Extract, Honeycomb Extract, Anaaaosa Extract, Seagrass Extract, Souhahi Extract, Zizyphus joazeiro Extract, Beech Extract, Kidachi Aloe Extract, Mannen Extract, Ginkgo Extract, Horsetail Extract, Safflower Extract , Ginseng extract, corn Cotton extract, Hagoromogusa extract, Astragalus extract, Mango extract, Cherimoya extract, Mangosteen extract, Tabebuia intiginosa extract, Yeast extract, Eggshell membrane extract protein, Deoxyribonucleic acid potassium salt, Lotus fermented liquid, Water eggplant extract, purple orchid root extract, murasakixikibu extract, rice extract, coral grass extract, pollen cargo extract and the like.

  Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, O-alkylascorbic acids such as 3-O-ethylascorbic acid, L-ascorbic acid-2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid) , L-ascorbic acid -Ascorbic acid sugar derivatives such as glucoside (5-O-α-D-glucopyranosyl-L-ascorbic acid), 6-position acylated products of these ascorbic acid sugar derivatives (acyl groups include hexanoyl group, octanoyl group, decanoyl group, etc.) L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetrafatty acid ester such as L-ascorbic acid tetralaurate, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, etc. Examples of hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside) and α-arbutin (hydroquinone-α-D-glucopyranoside), and examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol. 2 Examples of the 5-dihydroxybenzoic acid derivative include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid. Examples of the nicotinic acid derivative include nicotinic acid. Amides, benzyl nicotinate and the like, vitamin E derivatives such as vitamin E nicotinate and vitamin E linoleate, and α-hydroxy acids such as lactic acid, malic acid, succinic acid, citric acid, α-hydroxyoctanoic acid and the like There is.

  Next, the present invention will be described more specifically with reference to production examples, test examples and formulation examples (cosmetic examples), but the present invention is not limited thereto. In the following, all parts are by weight, and all% are by weight.

Production Example 1 (Processed activated carbon of sea cucumber extract)
Fresh American Duck (Zostera
marina) was thoroughly washed and dried in the sun and chopped. 20 g of this dried product was extracted for 7 days at room temperature using 1 kg of a solvent of water and ethanol (volume ratio 100: 7). This was filtered, and powdered activated carbon was added at 2% by weight of the filtrate and stirred for 10 minutes. This solution was filtered to obtain about 800 g of a pale yellow processed product (solid content 0.15% by weight).

Production Example 2 (Amamo extract treated with activated carbon)
Fresh American Duck (Zostera
marina) was thoroughly washed and dried in the sun and chopped. 1 kg of water was added to 50 g of this dried product, and the mixture was extracted by heating at 80 ° C. for 2 hours. This was filtered, powdered activated carbon was added at 1% of the weight of the filtrate, and the mixture was stirred for 40 minutes. This solution was filtered to obtain about 720 g of a slightly yellow processed product (solid content: 0.7% by weight).

Production Example 3 (Yama fermented yeast fermented product)
Fresh American Duck (Zostera
The whole plant of marina) was washed thoroughly, dried in the sun, and chopped. 950 g of water was added to 50 g of this dried product, and the mixture was extracted by heating at 80 ° C. for 2 hours. This was filtered and sterilized by heating at 85 ° C. for 1 hour, then inoculated with 10 ⁸ cells / mL of marine yeast (Saccharomyces cerevisiae), and statically cultured at 37 ° C. for 3 days. After completion of the culture, the culture solution was sterilized by heating and cooled to room temperature. This solution was filtered to obtain about 700 g of a yellow yeast fermented product (solid content: 1.4% by weight).

Production Example 4 (Enzyme Treatment / Yeast Fermentation / Activated Carbon Treatment of Amamo Extract)
Fresh American Duck (Zostera
The whole plant of marina) was washed thoroughly, dried in the sun, and chopped. 950 g of water was added to 50 g of this dried product, and the mixture was extracted by heating at 80 ° C. for 2 hours. After filtration and sterilization by heating at 85 ° C. for 1 hour, 0.5% of cellulase and 0.5% of pectinase are added to the amount of solids in the solution, and 10% of marine yeast (Saccharomyces cerevisiae) is added. ⁸ cells / mL were inoculated and statically cultured at 37 ° C. for 3 days. After completion of the culture, the culture solution was sterilized by heating and cooled to room temperature. This liquid was filtered, powdered activated carbon was added at 1% of the weight of the filtrate, and the mixture was stirred for 60 minutes. The activated carbon treatment liquid was filtered to obtain about 600 g of a pale yellow enzyme treatment / yeast fermentation / activated carbon product (solid content 1.2% by weight).

Production Example 5 (Lactic Acid Bacteria Fermentation Treatment of Amamo Extract)
Instead of yeast, marine lactic acid bacteria (Marinilactobacillus
phychrotolerans) in the same manner as in Production Example 3, except that yellow lactic acid bacteria fermented product (solid content: 1.3)
About 700 g (% by weight) was obtained.

Production Example 6 (Lactobacillus-treated fermented eel extract)
About 700 g of a yellow lactic acid bacteria fermented product (solid content 1.3 wt%) was obtained in the same manner as in Production Example 3 except that plant lactic acid bacteria (Lactobacillus plantarum) were used instead of yeast.

Production Example 7 (Anatto fermented natto fermentation product)
About 710 g of a yellow fermented natto fermentation product (solid content: 1.4% by weight) was obtained in the same manner as in Production Example 3 except that Bacillus subtilis was used instead of yeast.

Production Example 8 (Tempe Bacteria Treated Product of Amamo Extract)
Instead of yeast, Tempe fungus (Rhizopus microsporus
Except for the use of oligosporus), the yellow Tempe fermentation product (solids content 1.3
About 700 g (% by weight) was obtained.

Production Example 9 (Processed nonionic porous resin of sea cucumber extract)
Fresh American Duck (Zostera
marina) was thoroughly washed and dried in the sun and chopped. 20 g of this dried product was extracted for 7 days at room temperature using 1 kg of a solvent of water and ethanol (volume ratio, 100: 7). This was filtered, nonionic porous resin (Mitsubishi Chemical styrene-divinylbenzene resin Diaion HP-20) was added at 3% of the weight of the filtrate, and the mixture was stirred for 60 minutes. The solution was filtered to obtain a pale yellow extract (solid content 0.18
About 780 g (% by weight) was obtained.

Production Example 10 (Processed nonionic porous resin of sea cucumber extract)
Fresh American Duck (Zostera
marina) was thoroughly washed and dried in the sun and chopped. 20 g of this dried product was extracted for 7 days at room temperature using 1 kg of a solvent of water and ethanol (volume ratio, 100: 7). This was filtered, nonionic porous resin (Mitsubishi Chemical styrene weakly basic resin WA-30) was added at 3% of the weight of the filtrate, and the mixture was stirred for 60 minutes. This liquid was filtered to obtain a pale yellow processed product (solid content 0.18).
Weight%) about 760 g was obtained.

Production Example 11 (Ultrafiltration of Amamo extract)
The whole plant of fresh eelgrass was washed thoroughly, dried in the sun and chopped. 20 g of this dried product was extracted for 7 days at room temperature using 1 kg of a solvent of water and ethanol (volume ratio, 100: 7). After filtering this, MWCO
Ultrafiltration treatment was performed using a 50,000 ultrafiltration membrane to obtain about 720 g of a pale yellow processed product (solid content 0.17% by weight).

Production Example 12 (Amamo extract treated with activated carbon)
First, a eelgrass extract was obtained by the method described in Preparation Example 1 of Patent Document 1. That is, after freshly washed whole eel (Zostera marina) was thoroughly washed and dried in the sun, 20 g of this dried product was used with 1 kg of a mixture of water and ethanol (volume ratio, 3: 1). It was extracted for 7 days under room temperature conditions to obtain an eelgrass extract. Next, 2% of the weight of the extract was added to the obtained extract and stirred for 10 minutes. This solution was filtered to obtain about 840 g of a pale yellow processed product (solid content 0.13% by weight).

Comparative Production Example 1 (Amamo extract (Preparation Example 1 of Patent Document 1))
Fresh American Duck (Zostera
marina) was thoroughly washed and dried in the sun and chopped. 20 g of this dried product was extracted at room temperature for 7 days using 1 kg of a solvent of water and ethanol (volume ratio, 3: 1) at room temperature, and the resulting solution was filtered to obtain a pale green brown extract (solid content 0.15 content
About 900 g was obtained.

Formulation Example 1 Cream [Component A] Liquid paraffin 5.0
Hexalan (Note 1) 4.0
Paraffin 5.0
Glyceryl monostearate 2.0
Polyoxyethylene (20) sorbitan monostearate 6.0
Butylparaben 0.1
(Note 1) Technoble Co., Ltd. glyceryl trioctanoate

[B component]
Processed product of Production Example 1 10.0
Glycerin 5.0
Carboxymethyl monostearate 0.1
Moiston C (Note 2) 1.0
Amount of purified water totaling 100 parts (Note 2) NMF component manufactured by Technoble Co., Ltd.
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After this was cooled to 50 ° C., component C was added and further stirred and mixed to obtain a cream.

Formulation Example 2 Emulsion [component A] part liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Processed product of Production Example 2 5.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Formulation Example 3 Emulsion An emulsion was obtained in the same manner as in Formulation Example 2, except that the processed product of Production Example 3 was used instead of the processed product of Production Example 2 in the B component of Formulation Example 2.

Formulation Example 4 Emulsion An emulsion was obtained in the same manner as in Formulation Example 2, except that the processed product of Production Example 4 was used instead of the processed product of Production Example 2 in the B component of Formulation Example 2.

Formulation Example 5 Emulsion An emulsion was obtained in the same manner as in Formulation Example 2, except that the processed product of Production Example 5 was used instead of the processed product of Production Example 2 in the B component of Formulation Example 2.

Formulation Example 6 Emulsion An emulsion was obtained in the same manner as in Formulation Example 2 except that the fermented product of Production Example 6 was used instead of the processed product of Production Example 2 in the B component of Formulation Example 2.

Formulation Example 7 Emulsion An emulsion was obtained in the same manner as in Formulation Example 2 except that the treatment product of Production Example 9 was used instead of the treatment product of Production Example 2 in the B component of Formulation Example 2.

Formulation Example 8 Emulsion An emulsion was obtained in the same manner as in Formulation Example 2, except that the processed product of Production Example 10 was used instead of the processed product of Production Example 2 in the B component of Formulation Example 2.

Formulation Example 9 Lotion [component] part Processed product of Production Example 1 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Perfume
Potassium hydroxide appropriate amount Purified water Amount that makes 100 parts in total
The above ingredients were mixed to obtain a lotion.

Formulation example 10. Lotion A lotion was obtained in the same manner as in Formulation Example 9 except that the treated product of Production Example 4 was used instead of the treated product of Production Example 1 in the ingredients of Formulation Example 9.

Formulation Example 11 Essence [Ingredients] part Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Processed product of Production Example 4 60.0
Citric acid 0.3
Sodium citrate 0.6
Purified water in an amount of 100 parts Hyaluronic acid was dissolved in purified water, and then the remaining raw materials were sequentially added and dissolved by stirring to obtain a transparent essence.

Formulation Example 12. Essence Essence was obtained in the same manner as in Formulation Example 11 except that the processed product of Production Example 4 was used instead of the processed product of Production Example 1 among the components of Formulation Example 11.

Formulation Example 13 Lotion [component A] part olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
[B component]
Processed product of Production Example 1 10.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Potassium hydroxide appropriate amount Purified water Amount that makes 100 parts in total
[C component]
Perfume
After each component A and component B was heated to 80 ° C. or higher, the component B was added to the component A and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.

Formulation Example 14. Emulsion [component A] part liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Processed product of Production Example 4 5.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Formulation Example 15. Emulsion In addition to using 2.0 parts of L-ascorbic acid-2-phosphate and 2.0 parts of L-ascorbic acid-2-phosphate in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the component B of Formulation Example 14. Gave a milky lotion in the same manner as in Formulation Example 14.

Formulation Example 16. Emulsion In addition to using 2.0 parts of L-ascorbic acid-2-glucoside and 2.0 parts of sodium L-ascorbic acid-2-phosphate in place of component B in formulation example 14 and 0.5 parts of potassium hydroxide Gave a milky lotion in the same manner as in Formulation Example 14.

Formulation Example 17. Emulsion In the component B of Formulation Example 14, the emulsion was prepared in the same manner as Formulation Example 14 except that 2.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide. Obtained.

Example 18 Liquid foundation [component A] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[B component]
Processed product of Production Example 2 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount that makes the total amount 100 parts [C component]
Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount The components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.

Formulation Example 19. Cream foundation [component A] part Stearic acid 5.0
Cetanol 2.0
Glyceryl monostearate 3.0
Liquid paraffin 5.0
Squalane 3.0
Isopropyl myristate 8.0
Polyoxyethylene (20) glyceryl monostearate 2.0
Propylparaben 0.1
[B component]
Processed product of Production Example 3 5.0
Sorbitol 3.0
1,3-butylene glycol 5.0
Triethanolamine 1.5
Methylparaben 0.1
Purified water Amount that makes the total amount 100 parts [C component]
Titanium oxide 8.0
Talc 2.0
Kaolin 5.0
Bentonite 1.0
Coloring pigment appropriate amount [D component]
Fragrance 0.3
Component C was mixed and pulverized with a pulverizer. The component B was mixed, and the pulverized component C was added thereto and uniformly dispersed in a colloid mill. The components A and B and C dispersed uniformly were each heated to 80 ° C., and then the components A were added to the components B and C while stirring, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, D component was added and stirred and mixed, and also it cooled to 30 degrees C or less, stirring, and obtained the cream foundation.

Formulation Example 20. Body shampoo [component A] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[B component]
Processed product of Production Example 4 10.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a body shampoo It was.

Formulation Example 21. Soap [Component A] Part Hardened castor oil 26.0
Coconut oil 10.0
Olive oil 4.0
[B component]
Sodium hydroxide 6.0
Sugar 10.0
Glycerin 5.0
Processed product of Production Example 4 10.0
Purified water Amount that makes the total amount 100 parts [C component]
Ethanol 20.0
Perfume Appropriate A component and B component were each heated to 80 ° C. and dissolved uniformly, and then B component was added to A component to saponify. This was cooled to 50 ° C. with stirring, and component C was added. This was poured into a mold and cooled, and then dried at room temperature for several days. A sufficiently dried product was taken out from the mold to obtain soap.

Formulation Example 22 (hair lotion)
[component]
Part
Tragacanth gum
2.0
Glycerin 1.0
Ethanol 20.0
Methylparaben 0.2
Processed product of Production Example 1 10.0
Fragrance
Very small amount
purified water
Amount that the total amount is 100.0 parts
The above ingredients were mixed and stirred to obtain a uniform hair set lotion.

Formulation Example 23 (Hair Lotion)
A hair lotion was obtained in the same manner as in Formulation Example 22 except that the treatment product of Production Example 4 was used instead of the treatment product of Production Example 1 in the components of Formulation Example 22.

Formulation Example 24 (hair lotion)
A hair lotion was obtained in the same manner as in Formulation Example 22 except that the treatment product of Production Example 9 was used instead of the treatment product of Production Example 1 in the components of Formulation Example 22.

Formulation Example 25 (hair lotion)
A hair lotion was obtained in the same manner as in Formulation Example 22, except that the processed product of Production Example 11 was used instead of the processed product of Production Example 1 in the components of Formulation Example 22.

Formulation Example 26 (Hair Lotion)
A hair lotion was obtained in the same manner as in Formulation Example 22 except that the treatment product of Production Example 12 was used instead of the treatment product of Production Example 1 in the components of Formulation Example 22.

Comparative Formula 1 (Hair Lotion)
A hair lotion was obtained in the same manner as in Formulation Example 22 except that the extract of Comparative Production Example 1 was used in place of the processed product of Production Example 1 in the components of Formulation Example 22.

Test Example 1 (UV absorption)
Maximum absorption using a spectrophotometer for a solution obtained by diluting the treated product obtained in Production Examples 1 to 12 and the extract of Comparative Production Example 1 with purified water so as to have a solid content concentration of 0.04% by weight. The wavelength was measured. As a result, the extract of Comparative Production Example 1 showed UV absorption in both the UV-A and UV-B wavelength regions, as shown in FIG. 1 of Patent Document 1, as shown in FIG. As shown in FIGS. 1-12, the ultraviolet-ray absorptivity was not recognized by the processed material or fermented material of the manufacture examples 1-12 of this invention.

  From the above, by performing adsorption treatment with activated carbon or nonionic porous resin, ultrafiltration treatment, or fermentation treatment on the eelgrass extract, the ultraviolet absorption property that the eelgrass extract had has It is clear that it has disappeared.

Test example 2 (safety test (human patch test))
[Preparation of test substance and test sample]
Take 20 mL of each of the processed products of Production Examples 1 to 6, 9 to 12 and the extract of Comparative Production Example 1 into two 50 mL glass screw tubes, one as it is and the other as UV-A and An ultraviolet lamp that generates UV-B was irradiated with ultraviolet rays for 30 minutes (cumulative ultraviolet ray amount: 1260 mJ: equivalent to 30 minutes of sunlight outdoors in summer). The skin irritation of 10 kinds of test substances not irradiated with ultraviolet rays and 10 kinds of test substances irradiated with ultraviolet rays was investigated by a human patch test.
[Test method]
For 5 healthy subjects, fin chamber (EPITE-ST Ltd, Finland) pre-coated with 0.015 mL of each sample and Scampol tape (Alpharma
(A / S, Norway) was applied to the upper back skin of the subject for 24 hours.
Irritation was evaluated according to the method of Sugai (Tetsuro Sugai, Skin, 27, No. 4, 793-803 (1985)) that was considered based on Japanese standards.
[Table 1]

[Test results]
Table 2 shows the result of determination on irritation.
[Table 2]

  As shown in Table 2, among the 20 test substances, only the test substance that was irradiated with ultraviolet rays in Comparative Production Example 1 showed skin irritation. In the test substance of Comparative Production Example 1, since a UV absorption peak was observed on the UV absorption chart as shown in FIG. 13, it is considered that the contained component was excited by ultraviolet rays and changed into a stimulating substance. On the other hand, no irritation was observed in each of the test substances of Production Examples 1 to 6 and 9 to 12 regardless of the presence or absence of ultraviolet irradiation. Furthermore, the same effectiveness was shown also about the natto fermentation processed material of the manufacture example 7, and the Tempe bacteria fermentation processed product of the manufacture example 8.

  From the above, adsorption treatment with activated carbon or nonionic porous resin, ultrafiltration treatment, or fermentation treatment is considered to be very effective for improving safety, and the significance of these treatments was confirmed.

Test Example 3 (fibroblast activation effect)
Human dermis-derived fibroblasts NB1RGB (Lot.070512 (6)) were seeded at 1 × 10 ⁴ cells / hole in a 96-well microplate containing 0.5% NCS-containing Eagle's minimum essential medium, 37 ° C., 5. After pre-culturing for 1 day under the condition of 0% CO ₂ , the treated product of Production Examples 1 to 6, 9 to 12 and the extract of Comparative Production Example 1 were used as sample solutions in the medium at a concentration of 5.0% (solution And further cultured for 3 days under the same conditions. Next, the medium was removed, 0.03% MTT was added, and the mixture was kept at 37 ° C. for 1 hour, and the produced formazan was extracted with acidic isopropanol, and a microplate reader (Model 450, manufactured by Bio-Rad) was used. The MTT value was measured at a wavelength of 570-630 nm. In the case where no sample was added (control), the same operation as described above was performed, and the relative value of the MTT value at the time of adding each sample to the MTT value obtained here was determined to obtain the fibroblast MTT activity rate (%). . Moreover, in order to confirm whether the test system is functioning normally, the same test was also performed when 100 mM glucose was added instead of the sample solution (positive control).

The results of the test are shown in Table 3.
[Table 3]

  As shown in Table 3, the effect of activating the fibroblasts was not observed in the eel extract (Comparative Production Example 1), but when the activated carbon treatment was performed on the extract (Production Examples 1, 2, 12) ), When the nonionic porous resin treatment is performed (Production Examples 9 and 10), when the ultrafiltration treatment is performed (Production Example 11), and when the fermentation treatment is performed (Production Examples 3 to 6) Showed a fibroblast activation effect. Furthermore, the same effectiveness was shown also about the fermented natto fermentation product of the manufacture example 7, and the tempe fungus fermentation treatment product of the manufacture example 8. As a result, the fibroblast activation effect that was not observed in the eelgrass extract (non-treated product) was applied to the extract by adsorption treatment with activated carbon or nonionic porous resin, ultrafiltration treatment, or fermentation. It was confirmed that it was newly created by processing.

Test Example 4 (Tyrosinase activity inhibitory effect; tyrosine-tyrosinase reaction method)
First, 1.0 mg of tyrosinase (2200 units) was accurately weighed and dissolved in 2.0 mL of phosphate buffer (pH 6.8) to prepare a tyrosinase solution.
Next, 0.8 mL of an aqueous solution obtained by diluting the processed products obtained in Production Examples 1 to 6 and 9 to 12 by 10 times was accurately weighed, and 0.05 mL of a 0.05% L-tyrosine solution and phosphoric acid were added thereto. 1.0 mL of buffer solution (pH 6.8) was added and mixed well. 0.2 mL of the tyrosinase solution was added to this solution and mixed well. The absorbance of this solution at a wavelength of 475 nm was immediately measured, and then placed in a thermostatic bath at 37 ° C. After 24 minutes, this solution was taken out from the thermostat, the absorbance at a wavelength of 475 nm was measured again, and the tyrosinase activity index was determined from the following equation. Moreover, the thing operated similarly using water instead of the processed material was made into the blank.
Tyrosinase activity index = [(T ₂₄ −T ₀ ) / (B ₂₄ −B ₀ )] × 100 (%)
(In the formula, T ₂₄ is the absorbance of a solution to which a treated product after 24 minutes has elapsed since the start of the test, B ₂₄ is the absorbance of a solution to which water has been added instead of the treated product after 24 minutes from the start of the test, T ₀ represents the absorbance of the solution to which the treated product was added immediately after the start of the test, and B ₀ represents the absorbance of the solution to which water was added instead of the treated product immediately after the start of the test).

The results of Test Example 4 are shown in Table 4.
[Table 4]

  As described above, the treated products of Production Examples 1 to 6 and 9 to 12 showed a markedly excellent tyrosinase activity inhibitory effect as in Comparative Production Example 1 (non-treated product). Furthermore, the same effectiveness was shown also about the fermented natto fermentation product of the manufacture example 7, and the tempe fungus fermentation treatment product of the manufacture example 8. As a result, it was confirmed that the tyrosinase activity inhibitory effect could be maintained even when the adsorption of the sea cucumber was subjected to adsorption treatment with activated carbon or nonionic porous resin, ultrafiltration treatment or fermentation treatment. .

Test Example 5 (lipid peroxide production inhibitory action)
First, 0.5M ethanol linoleate 1.0mL, 0.2M phosphate buffer
(pH 7.0) 10 mL and ethanol 9.0 mL were each accurately weighed and sufficiently shaken and mixed in an Erlenmeyer flask with a stopper. To this liquid, 5.0 mL of a 5% aqueous solution of the processed products of Production Examples 1 to 6 and 9 to 12 accurately weighed was added and sufficiently mixed. 0.1 mL each of the solution immediately after preparation of this solution and the one left in a constant temperature bath at 40 ° C. for 7 days was accurately weighed, and then 4.7% of 75% ethanol and 30% ammonium thiocyanate solution 0 Add 1mL and mix well, then add 0.02M ferrous chloride 13.5% hydrochloric acid solution, measure the absorbance at 500nm exactly 3 minutes later, and calculate the peroxide number index from the following formula. It was.
Moreover, the thing operated similarly using water instead of the processed material was made into the blank.
Peroxide value index = [(T ₇ −T ₀ ) / (B ₇ −B ₀ )] × 100 (%)
(In the formula, T7 is the absorbance of the solution to which the treated product after 7 days has passed since the start of the test, B ₇
Is the absorbance of the solution to which water was added instead of the treated product 7 days after the start of the test, T ₀ is the absorbance of the solution to which the treated solution was added immediately after the start of the test, and B ₀ is the treated product immediately after the start of the test. Instead, it shows the absorbance of the solution to which water was added).

The test results are shown in Table 5.
[Table 5]

  As described above, the treated products of Production Examples 1 to 6 and 9 to 12 showed a markedly excellent lipid peroxide production inhibitory effect as in Comparative Production Example 1 (non-treated product). Furthermore, the same effectiveness was shown also about the fermented natto fermentation product of the manufacture example 7, and the tempe fungus fermentation treatment product of the manufacture example 8. As a result, it was confirmed that the lipid peroxide production inhibitory effect could be maintained even if the extraction of sea cucumber was subjected to adsorption treatment with activated carbon or nonionic porous resin, ultrafiltration treatment, or fermentation treatment. It was done.

Test Example 6 (humidity retention test; sample load test)
A test zone is set on the inner side of the forearm of subjects (5 men and women aged 26 to 38), and the stratum corneum moisture content in each test zone is measured (before application). Next, each test site (test section) was loaded with 100 μL of the processed products of Production Examples 1 to 6, 9 to 12 and the aqueous solution (solid content, 0.1%) of the extract of Comparative Production Example 1 as test solutions. (Drip) and lightly wipe after 10 seconds and immediately measure the stratum corneum moisture content. Then, the stratum corneum moisture content is measured every 20 seconds until 120 seconds (measuring instrument: SKIN SURFACE HYGROMETER (SKICON-200) manufactured by IBS). The conductance value after 120 seconds is compared with the conductance value before application, and the sustainability of the moisture retention performance is evaluated. The treatment is similarly performed three times and an average is taken. The positive control was a 5% aqueous solution of glycerin used as a moisturizer for cosmetic ingredients.

The results of the test are shown in Table 6.
[Table 6]

  The processed products of Production Examples 1 to 6 and 9 to 12 each have a conductance ratio of about 1.6 to 1.8 after 120 seconds, a 5% glycerin aqueous solution as a control, and a eel extract with a known moisturizing effect. The water retention was almost the same as that of the product (Comparative Production Example 1). Furthermore, the same effectiveness was shown also about the fermented natto fermentation product of the manufacture example 7, and the tempe fungus fermentation treatment product of the manufacture example 8. From the above results, the processed product obtained by subjecting the eelgrass extract to adsorption treatment with activated carbon or a nonionic porous resin, ultrafiltration treatment, or fermentation treatment is the untreated product of Comparative Production Example 1. It was confirmed that it has the same high moisturizing property as the eel extract.

Test example 7 (hair cosmetic test trial (half head monitor test))
Formulation Example 22 (hair lotion blended with the activated carbon product of Production Example 1), Formulation Example 23 (hair lotion blended with the fermented product of Production Example 4), Formulation Example 24 (Nonionic porous resin of Production Example 9) Hair lotion containing treated product), Formulation Example 25 (hair lotion containing the ultrafiltration product of Production Example 11), Formulation Example 26 (hair lotion containing the activated carbon treatment product of Production Example 12), and comparative formulation About Example 1 (hair lotion which mix | blended the eel extract (non-processed material) of the comparative manufacture example 1), the half head test shown below was each performed.

Targeting 50 randomly selected men and women aged 24 to 62 years old, 3 groups of 10 hair cosmetics once each day for 30 days for each hair. The glossiness, moist feeling, and combing were evaluated based on the following criteria.
[Glossy]
A: It became very shiny
B: Somehow shining
C: No change
D: Somehow lost glossiness
E: Clearly no gloss
[Moist feeling]
A: It feels very moist and feels better
B: Somehow moist and feel better
C: No change
D: Not very moist
E: There is no moist feeling
[Comb street]
A: Very good B: Somehow improved C: No change D: Somehow worse E: All worse

The test results of the half head are shown below.
[Table 7]

As shown in Table 7, even if the high evaluation hair improvement effect shown by the Amamo extract in the half head monitor test is performed by adsorption treatment with activated carbon or nonionic porous resin, ultrafiltration treatment, or fermentation treatment It became clear that it was maintained.

Claims (5)

  For extracts of plants belonging to the genus Zosteraceae (Zostera sp.), One or two of adsorption treatment with activated carbon or nonionic porous resin, ultrafiltration treatment and fermentation treatment A cosmetic comprising a processed product obtained by the above treatment.   The cosmetic composition according to claim 1, wherein the plant of the genus Zosteraceae is a genus Zostera marina.   The cosmetic according to claim 1 or 2, wherein the microorganism used for fermentation is at least one selected from yeast, lactic acid bacteria, natto bacteria, or tempeh bacteria.   The cosmetic according to claim 3, wherein Saccharomyces cerevisiae is used as the yeast. The cosmetic according to claim 3, wherein marine lactobacillus phychrotolerans derived from the ocean is used as the lactic acid bacterium.