JP5663523B2 - Method for producing pufferfish liver oil - Google Patents

Description

The present invention relates to a process for the preparation of liver oil nutritious containing many resulting functional components from the liver of non-toxic puffer puffer.

Since puffer's liver contains poisons such as tetrodotoxin, it could not be used for food.
Therefore, the present inventor has conducted earnest research and invented a method capable of detoxifying puffer fish by culturing under an environment where bacteria containing bacteria producing puffer venom are blocked (patent documents 1 and 2). .
However, in the case of aquatic non-toxic pufferfish, the liver cannot be used for food as well, and there has been a demand for the development of an effective usage method. In order to satisfy this demand, (Patent Document 3) states that “a plurality of puffer's livers extracted from a plurality of individual puffers are homogenized by stirring and mixing after the fiber is cut into small pieces, and from the stirring mixture. Extract a part of the extracted puffer liver mixture into an oil layer and an aqueous layer in a temperature range of 20 to 60 ° C., extract a sample from the separated aqueous layer, and extract the extracted aqueous layer sample In addition, after extracting a part of the specimen for toxicity test, add the emulsifier that prevents separation to the mixture of the separated pufferfish liver, and stir and mix again to homogenize the processed pufferfish liver product Technology is disclosed.
In addition, as a method for using puffer fish, (Patent Document 4) describes that “it is produced using cis9, trans11-conjugated linoleic acid and fish oil such as pufferfish as a composition for improving fat metabolism. “The composition for improving fat metabolism is to be a healthy (auxiliary) food” is disclosed, and (Patent Document 5) discloses “a method of reacting puffer meat with a protease to obtain an extract”. However, there is no description or suggestion about the use of the puffer's liver, and the puffer's liver is not fully utilized even though it contains active ingredients.
On the other hand, as fish liver, liver oil such as shark, ray, cod, sunfish and anglerfish is known as a health (auxiliary) food containing a large amount of n-3 fatty acids in polyunsaturated fatty acids such as docosahexaenoic acid and eicosapentaenoic acid. ing. As a method for producing these liver oils, generally, as in (Patent Document 6), “shark liver is shredded, heated to 50 ° C. to 70 ° C., oil and fat are obtained by centrifugation, and activated carbon treatment is performed. The process for producing highly unsaturated fatty acid-containing fats and oils according to claim 1, characterized in that the highly unsaturated fatty acid-containing fats and oils are obtained by performing a deodorizing treatment, and degradation of active ingredients such as fatty acids and the like It is disclosed to heat, extract and sterilize so as not to cause alteration.

JP 2004-24006 A JP 2004-16234 A JP 2006-214742 A JP 2007-314492 A JP 2010-516669 A JP 2002-265975 A

However, the above conventional techniques have the following problems.
(1) The technologies disclosed in (Patent Document 1) and (Patent Document 2) describe the possibility of using detoxified pufferfish liver as food, but describe and suggest specific processing methods It has not been done. Further, at present, provision and sale as food are not permitted, and other usage methods have been desired.
(2) The technique disclosed in (Patent Document 3) uses a puffer's liver, but it has a problem that liver oil cannot be collected because it is a processed product obtained by solidifying the liver other than the water used in the analysis. Was.
(3) The technology disclosed in (Patent Document 4) does not describe or suggest the type or collection method of fish oil and contains cis9, trans11-conjugated linoleic acid separately from fish oil, and the linoleic acid Has a problem of lack of productivity because it needs to be prepared separately.
(4) (Patent Document 5) discloses a technique, which has been extracted from puffer fish meat, processed how does a blowfish poison, no any mention or to discharged out of the system, also, polyhydric There was a problem that puffer's liver oil containing many active ingredients such as n-3 fatty acids in unsaturated fatty acids could not be used, and the liver could not be used effectively.
(5) In the method for producing liver oil disclosed in (Patent Document 6), the oil layer and other layers are separated by centrifugation after chopping the liver, so when a puffer's liver is used, a section is formed in the oil layer. Since it tends to cause rot, it requires a work to remove the section, which is cumbersome.In particular, if a section of the liver remains, there is a problem that safety is harmed by puffer poison in the section. Was.

The present invention solves the above-mentioned conventional problems, effectively uses the puffer's liver that has been disposed of, and contains active ingredients such as n-3 fatty acids in polyunsaturated fatty acids in a balanced manner, and , it is possible to produce oil efficiently active ingredient in n-3 fatty acids such as polyhydric in the unsaturated fatty acids from high content to that off grayed liver, n-3 in polyunsaturated fatty acids in the liver An object of the present invention is to provide a method for producing pufferfish liver oil, which can extract and sterilize liver oil without degrading or altering active ingredients such as fatty acids.

In order to solve the above problems, a manufacturing method of the liver oil of Puffer present invention has the following configuration.
In the method for producing puffer liver oil according to claim 1 of the present invention, the puffer liver confirmed to be non-toxic by individual toxicity test is housed in a vacuum pack as an extraction container, and is 60 ° C to 90 ° C. Extraction step of extracting the oil component by steaming at 60 to 90 ° C. in a water-permeable mounting part disposed on the upper part of the container of the low-temperature steamer that is an extraction container, and the liver A configuration comprising: a separation step of separating the oil and water by cooling the extracted container; a collection step of collecting the separated oil; and a sterilization step of heat-sterilizing the collected oil. Have.
With this configuration, the following effects can be obtained.
(1) Since oil is extracted by heating to 60 to 120 ° C., it can be sterilized at the same time as extraction, and deterioration of liver oil such as oxidation of unsaturated fatty acids due to heat hardly occurs. Can do.
(2) Since separation is performed by cooling to room temperature or lower, preferably 5 ° C. or lower, the water layer and the oil layer are separated quickly and the separability is excellent.
(3) Since it is heat sterilized, the safety of liver oil can be improved. The heat sterilization is preferably performed at 60 to 90 ° C. Since it is heat sterilized at 60 to 90 ° C., it can be sterilized without deteriorating liver oil, and high quality and excellent safety can be obtained.
(4) Since the liver is placed at the top of the extraction container, after extraction, the liver can be removed without stirring the liquid mixture of oil and water in the extraction container, and the liver can be removed simply by moving the mounting part. Since it can be removed, the time of the separation process can be shortened and the productivity is excellent.

In the extraction step, the heating temperature is suitably selected from 60 to 120 ° C, preferably 65 to 90 ° C. As the heating temperature becomes lower than 65 ° C., oil tends to be difficult to extract from the liver, and as the temperature becomes lower than 60 ° C., this tendency is remarkable. Also, as the temperature rises above 90 ° C., the oil tends to be easily deteriorated, such as the oxidation of unsaturated fatty acids, and as the temperature rises above 120 ° C., this tendency is remarkable.
In the separation step, the extracted liver is preferably removed from the extraction container before the oil is separated. This is to prevent liver sections from being mixed into the oil.
Separation is preferably performed in an environment of room temperature or lower, preferably 5 ° C. or lower. As the temperature becomes higher than 5 ° C., bacteria are likely to grow during the separation, and unsaturated fatty acids and the like contained in liver oil tend to be oxidized, which is not preferable. However, if there is no concern that bacteria will die and grow during extraction, separation may be performed under a temperature condition higher than 5 ° C.
As a separation method, any method can be used as long as the oil can be separated, and natural separation, centrifugation, or the like can be used.
The oil collecting method in the collecting process may be any method as long as the oil component separated and collected in the upper layer can be collected, such as a decantation method, a method of scooping with a cocoon-like object such as a ladle, siphon, A method of collecting using a liquid funnel can be used.
The collected oil is preferably filtered through a 30 to 100 mesh net as a filtration step. As the net for the filtration step, a metal such as stainless steel or a synthetic resin such as nylon can be used. When the collected oil contains coagulated bodies such as proteins, impurities, foreign matters, etc., these can be removed by the filtration step. Any method or method such as filter paper or centrifugal separation may be used as long as it can remove coagulation bodies such as proteins, impurities, foreign matters, and the like, as well as removal with a 30 to 100 mesh net.
In the sterilization step, the liver oil is preferably heated to 60 to 90 ° C. As the heating temperature becomes lower than 60 ° C, the sterilizing effect becomes weaker, the processing time becomes longer and the productivity tends to be lacking. As the heating temperature becomes higher than 90 ° C, the processing time can be shortened, but the deterioration of liver oil This is not preferable because the stability of the quality of the liver oil tends not to be obtained.
As the mounting portion, it is sufficient that the puffer's liver does not fall into the extraction container and the extracted oil can pass therethrough, and a net-like, colander-like, lattice-like, or slat-like shape can be used. .
Moreover, the atmosphere of an extraction container is heated at 60-120 degreeC, Preferably it is 65-90 degreeC. The material of the arrangement part may be formed of any material as long as it can withstand these temperatures, and metal, synthetic resin, wood, or the like can be used.
Liver puffer as the raw material, the kind of puffer if nontoxic regardless, it can be used puffer and Sabafugu, non-toxic in puffer fish, such as grass puffer. In the case of trough, the one whose liver is known to be non-toxic by a method such as aquaculture is used. Among them, the use of cultured trough puffs is preferable because individual inspection is easy and productivity is high.
Tetradotoxin (hereinafter referred to as TTX) and paralytic shellfish poison (hereinafter referred to as PSP) are known as puffer venoms. Since these puffer venoms are derived from the ingested food, Productivity can be increased by using pufferfish that has been cultivated isolated from the causative bait (benthic organisms).

The toxicity test for puffer liver is not particularly limited as long as it can confirm the presence or absence of poison, but after separating the oil from the liver and extracting the components of the poison into a solvent, the components other than the poison are solid-phased. After pretreatment such as extraction and removal, analysis can also be performed by a method such as high performance liquid chromatography.
The liver of the pufferfish is a live pufferfish or a fresh pufferfish disassembled and washed out with purified water. After removing blood, etc., the pufferfish is cut as it is or into 3 to 4 individuals, or the cuts are 5-6. What was put in place is used.
Extraction of liver oil uses a sputum, a water-permeable container, a mesh body, etc., the above-mentioned liver is accommodated in this, placed in the upper part of the extraction container, and steam adjusted to a temperature of 60-90 ° C. from above atomized hot water method peppered in evenly liver heat mist to extract liver oil (e.g. steam cooker, etc.) and, housed in vacuum packs, immerse the vacuum pack 60 to 90 ° C. hot water (hot water) A method or the like may be used. These methods are preferable because the liver is less likely to come into contact with oxygen in the air, and the extracted liver oil is less likely to be oxidized.
As the temperature of hot water, steam, etc. exceeds 90 ° C, fine protein coagulates are likely to occur, and the extracted oil tends to be easily oxidized. It is not preferable.

At the bottom of the extraction container, an extract composed of an aqueous solution in which liver oil and solids are mixed is collected. The aqueous solution in which liver oil and solid matter are mixed is separated into an oil layer and an aqueous layer by standing, but may be cooled to room temperature or lower during the separation. Further, for the separation, methods such as natural separation and centrifugation can be used.
The oil obtained is purified by filtration. If necessary, degumming treatment, dehydration treatment, deoxidation treatment, decolorization treatment, dewaxing treatment, filtration treatment and the like may be performed.
The refined liver oil can be encapsulated or used as it is as cooking oil.

  It is preferable to use vacuum hot water heating using a vacuum pack or the like because the oil is easily extracted and the oil is hardly oxidized by oxygen in the air. When the oil component is mixed with water or the like during extraction, the liver oil is collected in the upper layer by being left standing or subjected to a centrifuge, etc., and can be separated from water and other solid matters, thereby obtaining pure liver oil.

Puffer liver oil is a healthy (auxiliary) food, tablets, soft capsules, hard capsules, granules, solids, powders, pills, solvents, chewables, drinks, dressings, confectionery, seasonings (Oil) etc. can be used.
At this time, it may be mixed with excipients, extenders, thickeners, binders, emulsifiers, light amounts, acidulants, preservatives, antioxidants, colorants, food additives, seasonings, etc. Nutritional supplements such as vitamins, amino acids, protein, chitosan, reticin, sesamin may be added.

By heat treatment at 60 to 90 ° C., 22 to 30 mass% of saturated fatty acids, 35 to 45 monounsaturated fatty acids, and 27 to 35 mass% of polyunsaturated fatty acids were contained in the total amount of fatty acids as pufferfish liver oil. Things are obtained. Regarding the intake of fatty acids, it is desirable to take in the ratio of saturated fatty acid: monounsaturated fatty acid: polyunsaturated fatty acid = 3: 4: 3, and in the method for producing pufferfish liver oil of the present invention , The obtained liver oil has a composition close to this ratio, has a good balance of fatty acids, and the intake of fatty acids is not easily biased, so it can be used particularly suitably as a health (auxiliary) food such as a supplement .
The n-3 fatty acid in the polyunsaturated fatty acid is contained in an amount of 22 to 32 mass%.
In the case of aquaculture, the fatty acid content in liver oil is adjusted by changing the type of feed. For example, when it is desired to increase the content of the n-3 fatty acid in the polyunsaturated fatty acid , for example, fermented fish meal, commercially available fish meal feed, raw food such as horse mackerel, mackerel, and mami may be given.

It is preferable that n-6 type fatty acid in a polyunsaturated fatty acid is 5 mass% or less with respect to the total amount of fatty acid in liver oil. As the n-6 fatty acid in the polyunsaturated fatty acid is higher than 5 mass%, the intake of the n-6 fatty acid in the polyunsaturated fatty acid increases when the liver oil is ingested. This is not preferable because it tends to increase the possibility of causing health damage such as arteriosclerosis and hypertension. The puffer's liver oil extracted by steaming in a decompression kettle such as a vacuum pack treated at a low temperature of 60 to 90 ° C. or a low-temperature steamer has a low content of n-6 fatty acids in polyunsaturated fatty acids. Ingestion of n-6 fatty acids in polyunsaturated fatty acids is unlikely to become excessive even when ingested, and can be suitably used as a health (auxiliary) food or the like.

Trough pufferfish is known not to accumulate PSP among puffer venoms, so by using as a raw material the liver of trough puffer fish cultured in an environment isolated from benthic organisms containing bacteria that produce puffer venom, In another toxicity test, the toxicity test of PSP can be omitted, and it is only necessary to check the content of TTX, which is excellent in the efficiency and productivity of the toxicity test .
In addition, as a result of intensive studies by the inventor, it was found that the size of the liver becomes small when the testicles and ovaries begin to develop. There is concern that the active ingredient contained in the liver is decreasing due to the development of the testis and ovary. Therefore, by taking out the liver from the end of November to the beginning of February before the development of the testis and ovary, it is possible to suppress a decrease in the active ingredient, obtain a liver containing a large amount of the active ingredient, and improve the quality of the liver oil.
Moreover, the quality of liver oil can be improved by giving, for example, fermented fish meal, commercially available fish meal feed, raw food such as horse mackerel, mackerel, and mummy.

Liver oil manufacturing method of the full grayed is off grayed is a puffer farmed in an environment that is cut off from benthic organisms including bacteria produce a puffer poison, before the liver testis and ovary of the Fugu develops It is also possible to adopt a configuration that has been taken out .
With this configuration, in addition to the operation of the first aspect , the following operation is provided.
(1) Since the pufferfish venom accumulated in the body is TTX, it is only necessary to examine the content of TTX for tiger pufferfish cultivated in an environment isolated from benthic organisms including bacteria that produce puffer venom. Because of the high efficiency of toxicity tests, it is excellent in productivity.
(2) Since the liver is taken out before the testes and ovaries develop, it is possible to produce liver oil with a liver rich in active ingredients, and to obtain high-quality liver oil, and to collect oil before the liver becomes small Therefore, a large amount of liver oil can be collected, and the productivity is excellent.

The feed of the full grayed, high for example, fermented fish meal, commercial fish meal feed, horse mackerel, mackerel, by giving the raw bait Ami such, the quality of rich polyhydric n-3 fatty acids in the unsaturated fatty acids Liver oil can be obtained.

As described above, according to the manufacturing method of the liver oil of Puffer present invention, advantageous effects are obtained as follows.
According to the invention of claim 1,
(1) It is possible to provide a method for producing pufferfish liver oil that does not deteriorate unsaturated fatty acids, has high quality, high safety, and excellent productivity.
(2) It is possible to provide a method for producing pufferfish liver oil having a short oil separation time and excellent productivity.
(3) It is possible to provide a method for producing pufferfish liver oil, which is excellent in productivity and capable of obtaining high-quality liver oil.
(4) A method for producing pufferfish liver oil that can effectively utilize pufferfish liver, contains a large amount of DHA and EPA, and has a well-balanced fatty acid can be provided.
(5) It is possible to provide a method for producing pufferfish liver oil which has a good balance of fatty acids and can be suitably used as a health (auxiliary) food such as supplements.

Chromatogram showing TTX concentration of Example 1 Chromatogram showing TTX concentration of Comparative Example 1

Hereinafter, the present invention will be specifically described by way of examples. The present invention is not limited to these examples.
Example 1
In order to test the puffer's liver on an individual basis, first, prepare a pufferfish that has been cultivated in an environment isolated from benthic organisms containing bacteria that produce puffer's venom, before the testes and ovaries develop. The trough puffer liver that had been dismantled and removed was washed with water and blood was drawn. A portion of the washed liver was cut out and crushed in a mortar, and then 10.0 g of the liver was dispensed into a 50 mL capacity bottle and 20 mL of a 0.1 mass% acetic acid solution was added.
Next, the medium bottle was immersed in boiling water for 10 minutes, taken out, then immersed in water at about 0 ° C. and cooled to below room temperature, and the whole volume was adjusted to about 1 mL. The fixed volume liver mixture was centrifuged at 3500 rpm for 20 minutes using a centrifuge (manufactured by Kubota Corporation), and the separated upper layer oil was removed using a pipette to separate the liver extract. I took it.
Next, a C18 cartridge (BOND ELUT JR-C18 manufactured by VARIAN) was prepared. As the conditioning of the cartridge, 5 mL of methanol (manufactured by Kanto Chemical Co., Inc.) was flowed, and then 10 mL of a 0.1 mass% acetic acid solution was flowed. After conditioning, the whole liver extract was run to remove components other than TTX. The liquid flow rate during solid phase extraction was 4 mL / min. The liver extract cleaned up by solid phase extraction was filtered with DISMIC-25CS PTFE 0.45 μm (Toyo Roshi Kaisha, Ltd.) to obtain an analytical sample of Example 1.

(Comparative Example 1)
300 μL of 38.9 MU / mL TTX standard solution was added to 4.7 mL of the analysis sample of Example 1 to prepare a TTX concentration of 2.33 MU / mL, and an analysis sample of Comparative Example 1 was obtained.

(Fugula liver toxicity test)
The TTX concentration in the analysis samples of Comparative Example 1 and Example 1 was measured using a high performance liquid chromatographic fluorescence analysis method (hereinafter referred to as HPLC-FL analysis method). The injection amount of the analysis sample was 50 μL.
In the HPLC-FL analysis method, the mobile phase pump is L-2130 manufactured by Hitachi High-Technologies Corporation, the column is Puresil C18 (5 μm, 4.6 mmφ × 250 mm) manufactured by Waters, and the detector is manufactured by Hitachi High-Technologies Corporation. An L-2485 fluorescence detector was used.
The mobile phase was adjusted to pH 7.0 by adding 10 mM diammonium hydrogen phosphate to 10 mM ammonium dihydrogen phosphate, using a mixed solution prepared so that sodium 1-heptanesulfonate was 2.0 mM. The phase flow rate was set at 1.0 mL / min.
The column temperature was set to 25 ° C., and the reaction vessel (0.5 mmφ × 10 m made of Tefzel (registered trademark) resin) was set to 110 ° C. As a reaction solution, 4 mol / L sodium hydroxide was allowed to flow at a flow rate of 1.0 mL / min, and the analysis sample flowing along with the mobile phase was reacted. The results are shown in FIGS.

FIG. 1 is a chromatograph showing the TTX concentration of Example 1, and FIG. 2 is a chromatograph showing the TTX concentration of Comparative Example 1.
1 and 2, in Comparative Example 1, a peak appears at a retention time of 13.28 min, so that 13.28 min is considered to be a TTX peak. However, since no TTX peak was observed in Example 1, it was found that the analysis sample did not contain TTX (non-toxic).

(Example 2)
As an extraction process, aquaculture that was removed before the development of testes and ovaries that were fed fermented fish meal, commercially available fish meal feed, raw food such as horse mackerel, mackerel, and mite, and was confirmed to be non-toxic by toxicity tests 5 kg of tiger puffer's liver was prepared.
The prepared liver was washed with water to remove blood, placed on a 60-mesh wire net approximately evenly, and locked to the upper surface of a ball-shaped pot (extraction vessel) with a capacity of 10 L. Put the kettle with the trough puffer liver on the top surface in a heating device (Steamer (manufactured by Shinagawa Kogyo Co., Ltd.), keep it at 70 ± 2 ° C and let it stand for 4 hours to extract oil from the trough puffer's liver. Thus, a liver oil mixed solution in which liver oil and water were mixed was obtained.
Next, as a separation step, the kettle was taken out of the heating device, the trough puffer liver was removed together with the wire mesh, and the liver oil mixture accumulated in the kettle was stored in a refrigerator set at 5 ° C. or lower for 24 hours.
Next, as a collecting step, it was confirmed that the oil and water in the liver oil mixture were separated, and only the liver oil on the liquid surface side was collected with a ladle.
Moreover, as a sterilization process, the obtained liver oil was filled in a nylon poly bag, sealed, and then heat-sterilized at 63 ° C. for 30 minutes in a steamer (manufactured by Shinagawa Kogyo Co., Ltd.).
The obtained liver oil was used as the liver oil of Example 2. At this time, the oil sampled on February 13, 2009 and analyzed immediately after oil extraction was taken as Example 2-1, and the oil sampled on January 30, 2010 and analyzed immediately after oil extraction was taken as Example 2-2. .

(Measurement of fatty acid composition)
In the liver oil of Example 2, analysis was requested to the Japan Food Analysis Center, and the composition of fatty acids and vitamins per 100 g was analyzed.
In addition, foie gras and anchovy liver, which is said to contain a large amount of DHA among seafood, were compared with Example 2 on the basis of the 5th revised Japanese food standard ingredient table (issued by the Ministry of Education, Culture, Sports, Science and Technology). . The composition of the fatty acid was a ratio to the total amount of fatty acid. The results are shown in Table 1. Two samples of liver oil of Example 2 were analyzed.

From Table 1, since there is almost no difference in the composition of the two specimens in Example 2, it is considered that there is no individual difference in composition, and the stability of the quality of liver oil can be obtained by using cultured trough puffer It is considered a thing.
Foie gras accounts for 98.6 mass% of the total amount of fatty acids only with saturated fatty acids and monounsaturated fatty acids, does not contain n-3 fatty acids in polyunsaturated fatty acids, and liver of anglerfish and liver oil of Example 2 The composition was very different. When the liver oil of Example 2 and the liver of anglerfish are compared, the amount of saturated fatty acid is 25 mass% on average for the liver oil of example 2 and 23.1 mass% for the anglerfish liver, but there is almost no change in monounsaturated fatty acids. The difference of 51.6 mass% for anglerfish and 40.7 mass% on average for the liver oil of Example 2 was found to be about 10 mass%. Regarding polyunsaturated fatty acids, the liver of anglerfish is 23.6 mass%, the liver oil of Example 2 is about 32 mass%, and Example 2 contains more polyunsaturated fatty acids. I understood. Among the polyunsaturated fatty acids, EPA has 6.5 mass% for the angler liver and 6.2 mass% on average for the liver oil of Example 2, but there is almost no difference. Compared to 2 mass%, the average of the liver oil of Example 2 was 13.3 mass%, indicating that more DHA was contained than the liver of anglerfish.
Moreover, about vitamin A, the liver oil of Example 2-2 is contained about 1.5 times the liver of anglerfish and about 13 times the foie gras, and regarding vitamin E, the liver oil of Example 2-1 is About 2 times the liver of anglerfish, about 90 times more than foie gras, liver oil of Example 2-2 contains about 5 times more liver than anglerfish and about 220 times more than foie gras, and has a high vitamin content I understood.

  Next, Table 2 shows the contents of DHA, EPA, and vitamin E when 300 mg of the liver oil of Example 2 is sealed in a soft capsule made of porcine collagen.

In Table 2, the dietary intake standards are based on the Japanese dietary intake standards issued by the Ministry of Health, Labor and Welfare, and those 18 to 29 years old were excerpted.
From Table 2, the capsule encapsulating liver oil of Example 2 has less content of vitamin E and n-6 fatty acids in polyunsaturated fatty acids than the intake target, but ingests one capsule of vitamin A. It turned out that it is possible to take one day. In addition, n-3 fatty acids, DHA and EPA in polyunsaturated fatty acids can ingest 4 to 1/5 of the daily intake target by ingesting about 4 grains, so as a supplement It is estimated that can also be used suitably.

The present invention is to effectively utilize the liver puffer which has been discarded, comprising a well-balanced active ingredients such as polyhydric n-3 fatty acids in the unsaturated fatty acids, and, in full grayed you contains a large amount Effective ingredients such as n-3 fatty acids in polyunsaturated fatty acids can be efficiently extracted from the liver, and the active ingredients such as n-3 fatty acids in polyunsaturated fatty acids in liver oil are degraded. It is possible to provide a method for producing liver oil that can extract or sterilize liver oil without alteration.

Claims (1)

Heated by vacuum hot water in which the puffer's liver, which has been confirmed to be non-toxic by individual toxicological tests, is stored in a vacuum pack, which is an extraction container, and processed at 60 ° C. to 90 ° C. An extraction step of extracting oil by steaming at 60 to 90 ° C. at a water-permeable mounting portion disposed on the upper part of the tube, and separating the oil from water and the like by cooling the extraction container from which the liver has been removed A method for producing a pufferfish liver oil comprising: a separating step for collecting, a collecting step for collecting the separated oil component, and a sterilizing step for heat-sterilizing the collected oil component.

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