JP2013230113A - Globefish liver oil, and method for producing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide globefish liver oil containing a large amount of active ingredients such as n-3 fatty acid in a well-balanced manner while effectively utilizing discarded globefish livers, and a method for producing liver oil by which active ingredients such as n-3 fatty acid from globefish livers are obtained, and the liver oil is extracted and sterilized without degrading/deteriorating the active ingredients such as the n-3 fatty acid in the liver oil.SOLUTION: Globefish liver oil is extracted from globefish livers confirmed to be nontoxic by an individual toxicity examination.

Description

  The present invention relates to a nutritious pufferfish liver oil containing a large amount of functional components obtained from nontoxic pufferfish liver and a method for producing the same.

Since puffer's liver contains poisons such as tetrodotoxin, it could not be used for food.
For this reason, the present inventor has conducted earnest research and invented a method that can detoxify puffer fish by culturing in an environment that shuts off organisms containing bacteria that produce puffer poison. (Patent Documents 1 and 2)
However, in the case of aquatic non-toxic pufferfish, the liver cannot be used for food as well, and there has been a demand for the development of an effective usage method. In order to satisfy this demand, (Patent Document 3) states that “a plurality of puffer's livers extracted from a plurality of individual puffers are homogenized by stirring and mixing after the fiber is cut into small pieces, and from the stirring mixture. Extract a part of the extracted puffer liver mixture into an oil layer and an aqueous layer in a temperature range of 20 to 60 ° C., extract a sample from the separated aqueous layer, and extract the extracted aqueous layer sample In addition, after extracting a part of the specimen for toxicity test, add the emulsifier that prevents separation to the mixture of the separated pufferfish liver, and stir and mix again to homogenize the processed pufferfish liver product Technology is disclosed.
In addition, as a method for using puffer fish, (Patent Document 4) describes that “it is produced using cis9, trans11-conjugated linoleic acid and fish oil such as pufferfish as a composition for improving fat metabolism. “The composition for improving fat metabolism is to be a healthy (auxiliary) food” is disclosed, and (Patent Document 5) discloses “a method of reacting puffer meat with a protease to obtain an extract”. However, there is no description or suggestion about the use of the puffer's liver, and the puffer's liver is not fully utilized even though it contains active ingredients.
On the other hand, as a liver of fish, liver oil such as shark, ray, cod, sunfish and anglerfish is known as a health (auxiliary) food containing a lot of n-3 fatty acids such as docosahexaenoic acid and eicosapentaenoic acid. As a method for producing these liver oils, generally, as in (Patent Document 6), “shark liver is shredded, heated to 50 ° C. to 70 ° C., oil and fat are obtained by centrifugation, and activated carbon treatment is performed. The process for producing highly unsaturated fatty acid-containing fats and oils according to claim 1, characterized in that the highly unsaturated fatty acid-containing fats and oils are obtained by performing a deodorizing treatment, and degradation of active ingredients such as fatty acids and the like It is disclosed to heat, extract and sterilize so as not to cause alteration.

JP 2004-24006 A JP 2004-16234 A JP 2006-214742 A JP 2007-314492 A JP 2010-516669 A JP 2002-265975 A

However, the above conventional techniques have the following problems.
(1) The technologies disclosed in (Patent Document 1) and (Patent Document 2) describe the possibility of using detoxified pufferfish liver as food, but describe and suggest specific processing methods It has not been done. Further, at present, provision and sale as food are not permitted, and other usage methods have been desired.
(2) The technique disclosed in (Patent Document 3) uses a puffer's liver, but it has a problem that liver oil cannot be collected because it is a processed product obtained by solidifying the liver other than the water used in the analysis. Was.
(3) The technology disclosed in (Patent Document 4) does not describe or suggest the type or collection method of fish oil and contains cis9, trans11-conjugated linoleic acid separately from fish oil, and the linoleic acid Has a problem in that it lacks productivity.
(4) The technology disclosed in (Patent Document 5) is extracted from the meat of puffer fish, there is no mention of how puff poison is treated and discharged out of the system, and n- The puffer's liver oil which contains many active ingredients, such as 3 type | system | group fatty acid, was not able to be utilized, but it had the subject that the liver was not able to be utilized effectively.
(5) In the method for producing liver oil disclosed in (Patent Document 6), the oil layer and other layers are separated by centrifugation after chopping the liver, so when a puffer's liver is used, a section is formed in the oil layer. Since contamination tends to cause corruption, the section needs to be removed, and the work is complicated. Was.

The present invention solves the above-mentioned conventional problems, and effectively uses the liver of pufferfish that has been disposed of, and contains a large amount of active ingredients such as n-3 fatty acids in a balanced manner. The purpose is to provide liver oil.
In addition, it is possible to efficiently extract active ingredients such as n-3 fatty acids from the puffer's liver, and to extract and sterilize liver oil without degrading and altering active ingredients such as n-3 fatty acids in liver oil. It aims at providing the manufacturing method of the liver oil which can be performed.

In order to solve the above conventional problems, the pufferfish liver oil and the method for producing the same of the present invention have the following configurations.
The puffer's liver oil according to claim 1 of the present invention has a structure extracted from puffer's liver that has been confirmed to be non-toxic by individual toxicity tests.
With this configuration, the following effects can be obtained.
(1) Since active ingredients such as fatty acids and active substances can be obtained from the puffer's liver that cannot be used for food and discarded, the puffer's liver can be used effectively.
(2) Puffer liver oil contains more fatty acids such as docosahexaenoic acid (hereinafter referred to as DHA) and eicosapentaenoic acid (hereinafter referred to as EPA) than other fish, and saturated fatty acids and unsaturated fatty acids. Since it contains polyunsaturated fatty acids in a well-balanced manner, it can be suitably used as a supplement or the like.
(3) Functional components such as DHA and EPA that have been discarded together with the liver can be collected and used to improve human brain functions.
(4) Since liver oil can be encapsulated in capsules and the like, it can be easily consumed by using active ingredients in liver oil by using it as a raw material for supplements and the like, and can be suitably used for maintaining the health of animals including humans.

Here, as for the puffer liver used as a raw material, any non-toxic puffer such as trough pufferfish, mackerel pufferfish, or puffer puffer can be used as long as it is nontoxic. In the case of trough, the one whose liver is known to be non-toxic by a method such as aquaculture is used. Among them, the use of cultured trough puffs is preferable because individual inspection is easy and productivity is high.
Tetradotoxin (hereinafter referred to as TTX) and paralytic shellfish poison (hereinafter referred to as PSP) are known as puffer venoms. Since these puffer venoms are derived from the ingested food, Productivity can be increased by using pufferfish that has been cultivated isolated from the causative bait (benthic organisms).

The toxicity test for puffer liver is not particularly limited as long as it can confirm the presence or absence of poison, but after separating the oil from the liver and extracting the components of the poison into a solvent, the components other than the poison are solid-phased. After pretreatment such as extraction and removal, analysis can also be performed by a method such as high performance liquid chromatography.
For the pufferfish liver, the live pufferfish or fresh pufferfish is disassembled and the liver taken out is washed with purified water, and after removing blood, etc., it is cut as it is or into 3 to 4 individuals, or the cut is 5-6. What was put in place is used.
Extraction of liver oil uses a sputum, a water-permeable container, a mesh body, etc., contains the above-mentioned liver, arranges it in the upper part of the extraction container, and a temperature of 60 to 120 ° C., preferably 65 to 90 ° C. from above. A method of extracting liver oil by uniformly bathing the liver with steam, mist-like hot water or hot mist adjusted (eg, steam cooker), a method of extracting the liver by immersing it in hot water at 60 to 90 ° C, vacuum You may use the method etc. which are accommodated in a pack and a vacuum pack is immersed in 60-90 degreeC hot water (warm water). These methods are preferable because the liver is less likely to come into contact with oxygen in the air, and the extracted liver oil is less likely to be oxidized.
As the temperature of hot water, steam, etc. exceeds 90 ° C, fine protein coagulates are likely to occur, and the extracted oil tends to be easily oxidized. It is not preferable.

At the bottom of the extraction container, an extract composed of an aqueous solution in which liver oil and solids are mixed is collected. The aqueous solution in which liver oil and solid matter are mixed is separated into an oil layer and an aqueous layer by standing, but may be cooled to room temperature or lower during the separation. Further, for the separation, methods such as natural separation and centrifugation can be used.
The oil obtained is purified by filtration. If necessary, degumming treatment, dehydration treatment, deoxidation treatment, decolorization treatment, dewaxing treatment, filtration treatment and the like may be performed.
The refined liver oil can be encapsulated or used as it is as cooking oil.

  It is preferable to use vacuum hot water heating using a vacuum pack or the like because the oil is easily extracted and the oil is hardly oxidized by oxygen in the air. When the oil component is mixed with water or the like during extraction, the liver oil is collected in the upper layer by being left standing or subjected to a centrifuge, etc., and can be separated from water and other solid matters, thereby obtaining pure liver oil.

Puffer liver oil is a healthy (auxiliary) food, tablets, soft capsules, hard capsules, granules, solids, powders, pills, solvents, chewables, drinks, dressings, confectionery, seasonings (Oil) etc. can be used.
At this time, it may be mixed with excipients, extenders, thickeners, binders, emulsifiers, light amounts, acidulants, preservatives, antioxidants, colorants, food additives, seasonings, etc. Nutritional supplements such as vitamins, amino acids, protein, chitosan, reticin, sesamin may be added.

The invention according to claim 2 is the puffer's liver oil according to claim 1, wherein the saturated fatty acid is 22-30 mass% and the monounsaturated fatty acid is 35-45 mass% with respect to the total amount of fatty acid in the liver oil. The polyhydric fatty acid is contained in an amount of 27 to 35 mass%, and the n-3 fatty acid in the polyunsaturated fatty acid is contained in an amount of 22 to 32 mass% with respect to the total amount of the fatty acid.
With this configuration, the following operation is obtained in addition to the operation of the first aspect.
(1) Since the balance of fatty acids is good and the intake of fatty acids is not easily biased, it can be suitably used as a health (auxiliary) food such as supplements.

By heat treatment at 60 to 120 ° C., 22 to 30 mass% saturated fatty acid, 35 to 45 monounsaturated fatty acid, and 27 to 35 mass% polyunsaturated fatty acid with respect to the total amount of fatty acid as puffer's liver oil Things are obtained. Regarding the intake of fatty acids, it is desirable to take in the ratio of saturated fatty acid: monounsaturated fatty acid: polyunsaturated fatty acid = 3: 4: 3, and the puffer liver oil of the present invention has this ratio. It has a composition close to that, and can be used particularly suitably as a health (auxiliary) food.
The n-3 fatty acid in the polyunsaturated fatty acid is contained in an amount of 22 to 32 mass%.
In the case of aquaculture, the fatty acid content in liver oil is adjusted by changing the type of feed. For example, when it is desired to increase the content of n-3 fatty acids, for example, fermented fish meal, commercially available fish meal feed, raw food such as horse mackerel, mackerel, and mami may be given.

The invention according to claim 3 is the puffer's liver oil according to claim 1 or 2, wherein the content of n-6 fatty acid in the polyunsaturated fatty acid is 5 mass% or less with respect to the total amount of the fatty acid. It has a certain configuration.
With this configuration, in addition to the operation of the first or second aspect, the following operation is provided.
(1) In particular, puffer liver oil extracted by steaming in a decompression kettle or vacuum steamer treated at a low temperature of 60 to 90 ° C. has a low content of n-6 fatty acids. Even so, the intake of n-6 fatty acids is unlikely to be excessive, and can be suitably used as a health (auxiliary) food.

  It is preferable that n-6 type fatty acid is 5 mass% or less with respect to the total amount of fatty acid in liver oil. As n-6 fatty acids become higher than 5 mass%, the intake of n-6 fatty acids increases when liver oil is ingested, and there is a high possibility of causing health damage such as arteriosclerosis and hypertension when ingested excessively. This is not preferable.

Invention of Claim 4 is a liver oil of a puffer fish of any one of Claims 1 thru | or 3, Comprising: In the environment where the said pufferfish was shielded from the benthic organism containing the bacteria which produces a puffer venom. It is a farmed tiger puffer and has a structure in which the liver is taken out before the testes and ovaries of the tiger puffer are developed.
With this configuration, in addition to the operation of any one of claims 1 to 3, the following operation is provided.
(1) Since the pufferfish venom accumulated in the body is TTX, it is only necessary to examine the content of TTX for tiger pufferfish cultivated in an environment isolated from benthic organisms including bacteria that produce puffer venom. Excellent in toxicity test efficiency.
(2) Since the liver is taken out before the testis and ovaries develop, a liver rich in active ingredients can be obtained, and the quality of liver oil can be improved.

Trough pufferfish is known not to accumulate PSP among puffer venoms, so by using as a raw material the liver of trough puffer fish cultured in an environment isolated from benthic organisms containing bacteria that produce puffer venom, In another toxicity test, the toxicity test of PSP can be omitted, and the productivity is excellent.
In addition, as a result of intensive studies by the inventor, it was found that the size of the liver becomes small when the testicles and ovaries begin to develop. There is concern that the active ingredient contained in the liver is decreasing due to the development of the testis and ovary. Therefore, by taking out the liver from the end of November to the beginning of February before the development of the testis and ovaries, it is possible to suppress the decrease in active ingredients and improve the quality of liver oil.
Moreover, the quality of liver oil can be improved by giving, for example, fermented fish meal, commercially available fish meal feed, raw food such as horse mackerel, mackerel, and mummy.

6. The method for producing pufferfish liver oil according to claim 5, wherein the pufferfish liver, which has been confirmed to be non-toxic by individual toxicological tests, is heated to 60 ° C. to 120 ° C. in an extraction container to extract oil. A separation step of separating the oil and moisture by cooling the extraction container from which the liver has been removed, a sampling step of collecting the separated oil, and a sterilization step of heat-sterilizing the collected oil , Has a configuration comprising.
This configuration has the following effects.
(1) Since oil is extracted by heating to 60 to 120 ° C., it can be sterilized at the same time as extraction, and deterioration of liver oil such as oxidation of unsaturated fatty acids due to heat hardly occurs. Can do.
(2) Since separation is performed by cooling to room temperature or lower, preferably 5 ° C. or lower, the water layer and the oil layer are separated quickly and the separability is excellent.
(3) Since it is heat sterilized, the safety of liver oil can be improved. The heat sterilization is preferably performed at 60 to 90 ° C. Since it is heat sterilized at 60 to 90 ° C., it can be sterilized without deteriorating liver oil, and high quality and excellent safety can be obtained.

  In the extraction step, the heating temperature is suitably selected from 60 to 120 ° C, preferably 65 to 90 ° C. As the heating temperature becomes lower than 65 ° C., oil tends to be difficult to extract from the liver, and as the temperature becomes lower than 60 ° C., this tendency is remarkable. Also, as the temperature rises above 90 ° C., the oil tends to be easily deteriorated, such as the oxidation of unsaturated fatty acids.

In the separation step, the extracted liver is preferably removed from the extraction container before the oil is separated. This is to prevent liver sections from being mixed into the oil.
Separation is preferably performed in an environment of room temperature or lower, preferably 5 ° C. or lower. As the temperature becomes higher than 5 ° C., bacteria are likely to grow during the separation, and unsaturated fatty acids and the like contained in liver oil tend to be oxidized, which is not preferable. However, if there is no concern that bacteria will die and grow during extraction, separation may be performed under a temperature condition higher than 5 ° C.
As a separation method, any method can be used as long as the oil can be separated, and natural separation, centrifugation, or the like can be used.

  The oil collecting method in the collecting process may be any method as long as the oil component separated and collected in the upper layer can be collected, such as a decantation method, a method of scooping with a cocoon-like object such as a ladle, siphon, A method of collecting using a liquid funnel can be used.

  The collected oil is preferably filtered through a 30 to 100 mesh net as a filtration step. As the net for the filtration step, a metal such as stainless steel or a synthetic resin such as nylon can be used. When the collected oil contains coagulated bodies such as proteins, impurities, foreign matters, etc., these can be removed by the filtration step. Any method or method such as filter paper or centrifugal separation may be used as long as it can remove coagulation bodies such as proteins, impurities, foreign matters, and the like, as well as removal with a 30 to 100 mesh net.

  In the sterilization step, the liver oil is preferably heated to 60 to 90 ° C. As the heating temperature becomes lower than 60 ° C, the sterilizing effect becomes weaker, the processing time becomes longer and the productivity tends to be lacking. As the heating temperature becomes higher than 90 ° C, the processing time can be shortened, but the deterioration of liver oil This is not preferable because the stability of the quality of the liver oil tends not to be obtained.

Invention of Claim 6 is a manufacturing method of the puffer's liver oil of Claim 5, Comprising: The said extraction process arrange | positions the said liver in the arrangement | positioning part which has the water permeability arrange | positioned by the upper side of the said extraction container. However, it has the structure heated with steam.
With this configuration, in addition to the operation of the fifth aspect, the following operation is provided.
(1) Since the liver is placed in the upper part of the extraction container, after extraction, the liver can be removed without stirring the mixture of oil and water in the extraction container, and the liver can be removed simply by moving the placement part. Therefore, the time for the separation process can be shortened and the productivity is excellent.

As the arrangement portion, it is only necessary that the puffer liver does not fall into the inside of the extraction container and the extracted oil can pass through, and forms such as a net shape, a colander shape, a lattice shape, and a drainboard shape can be used.
Moreover, the atmosphere of an extraction container is heated at 60-120 degreeC, Preferably it is 65-90 degreeC. The material of the arrangement part may be formed of any material as long as it can withstand these temperatures, and metal, synthetic resin, wood, or the like can be used.

The invention according to claim 7 is the method for producing the pufferfish liver oil according to claim 5 or 6, wherein the pufferfish is cultivated in an environment isolated from benthic organisms containing bacteria that produce pufferfish poisons. And the liver is taken out before the development of the testes and ovaries of the tiger pufferfish.
With this configuration, in addition to the operation of the fifth or sixth aspect, the following operation is provided.
(1) Since the pufferfish venom accumulated in the body is TTX, it is only necessary to examine the content of TTX for tiger pufferfish cultivated in an environment isolated from benthic organisms including bacteria that produce puffer venom. Because of the high efficiency of toxicity tests, it is excellent in productivity.
(2) Since the liver is taken out before the testes and ovaries develop, it is possible to produce liver oil with a liver rich in active ingredients, and to obtain high-quality liver oil, and to collect oil before the liver becomes small Therefore, a large amount of liver oil can be collected, and the productivity is excellent.

The explanation about the time to extract the pufferfish poison and liver contained in the trough is the same as in the paragraph [0017], and the explanation is omitted.
Moreover, as a feed for puffer fish, for example, by giving a raw feed such as fermented fish meal, commercially available fish meal feed, horse mackerel, mackerel, ami, etc., it is possible to obtain liver oil having a high quality containing a lot of n-3 fatty acids and the like. .

As described above, according to the pufferfish liver oil and the method for producing the same of the present invention, the following advantageous effects can be obtained.
According to the invention of claim 1,
(1) The puffer's liver can be used effectively, can contain a lot of DHA and EPA, and can provide puffer's liver oil with a good balance of fatty acids.

According to invention of Claim 2, in addition to the effect of Claim 1,
(1) It is possible to provide pufferfish liver oil that has a good balance of fatty acids and can be suitably used as a health (auxiliary) food such as supplements.

According to invention of Claim 3, in addition to the effect of Claim 1 or 2,
(1) It is possible to provide pufferfish liver oil that is difficult to excessively consume n-6 fatty acids and can be suitably used as a health (auxiliary) food.

According to the invention of claim 4, in addition to the effect of any one of claims 1 to 3,
(1) It is possible to provide high-quality pufferfish liver oil containing a large amount of active ingredients.

According to invention of Claim 5, in addition to the effect of Claim 4,
(1) It is possible to provide a method for producing pufferfish liver oil that does not deteriorate unsaturated fatty acids, has high quality, high safety, and excellent productivity.

According to the invention described in claim 6, in addition to the effect of claim 5,
(1) It is possible to provide a method for producing pufferfish liver oil that has a short oil separation time and excellent productivity.

According to invention of Claim 7, in addition to the effect of Claim 5 or 6,
(1) It is possible to provide a method for producing a pufferfish liver oil that is excellent in productivity and can obtain a high-quality liver oil.

Chromatogram showing TTX concentration of Example 1 Chromatogram showing TTX concentration of Comparative Example 1

Hereinafter, the present invention will be specifically described by way of examples. The present invention is not limited to these examples.
Example 1
In order to test the puffer's liver on an individual basis, first, prepare a pufferfish that has been cultivated in an environment isolated from benthic organisms containing bacteria that produce puffer's venom, before the testes and ovaries develop. The trough puffer liver that had been dismantled and removed was washed with water and blood was drawn. A portion of the washed liver was cut out and crushed in a mortar, and then 10.0 g of the liver was dispensed into a 50 mL capacity bottle and 20 mL of a 0.1 mass% acetic acid solution was added.
Next, the medium bottle was immersed in boiling water for 10 minutes, taken out, then immersed in water at about 0 ° C. and cooled to below room temperature, and the whole volume was adjusted to about 1 mL. The fixed volume liver mixture was centrifuged at 3500 rpm for 20 minutes using a centrifuge (manufactured by Kubota Corporation), and the separated upper layer oil was removed using a pipette to separate the liver extract. I took it.
Next, a C18 cartridge (BOND ELUT JR-C18 manufactured by VARIAN) was prepared. As the conditioning of the cartridge, 5 mL of methanol (manufactured by Kanto Chemical Co., Inc.) was flowed, and then 10 mL of a 0.1 mass% acetic acid solution was flowed. After conditioning, the whole liver extract was run to remove components other than TTX. The liquid flow rate during solid phase extraction was 4 mL / min. The liver extract cleaned up by solid phase extraction was filtered with DISMIC-25CS PTFE 0.45 μm (Toyo Roshi Kaisha, Ltd.) to obtain an analytical sample of Example 1.

(Comparative Example 1)
300 μL of 38.9 MU / mL TTX standard solution was added to 4.7 mL of the analysis sample of Example 1 to prepare a TTX concentration of 2.33 MU / mL, and an analysis sample of Comparative Example 1 was obtained.

(Fugula liver toxicity test)
The TTX concentration in the analysis samples of Comparative Example 1 and Example 1 was measured using a high performance liquid chromatographic fluorescence analysis method (hereinafter referred to as HPLC-FL analysis method). The injection amount of the analysis sample was 50 μL.
In the HPLC-FL analysis method, the mobile phase pump is L-2130 manufactured by Hitachi High-Technologies Corporation, the column is Puresil C18 (5 μm, 4.6 mmφ × 250 mm) manufactured by Waters, and the detector is manufactured by Hitachi High-Technologies Corporation. An L-2485 fluorescence detector was used.
The mobile phase was adjusted to pH 7.0 by adding 10 mM diammonium hydrogen phosphate to 10 mM ammonium dihydrogen phosphate, using a mixed solution prepared so that sodium 1-heptanesulfonate was 2.0 mM. The phase flow rate was set at 1.0 mL / min.
The column temperature was set to 25 ° C., and the reaction vessel (0.5 mmφ × 10 m made of Tefzel (registered trademark) resin) was set to 110 ° C. As a reaction solution, 4 mol / L sodium hydroxide was allowed to flow at a flow rate of 1.0 mL / min, and the analysis sample flowing along with the mobile phase was reacted. The results are shown in FIGS.

FIG. 1 is a chromatograph showing the TTX concentration of Example 1, and FIG. 2 is a chromatograph showing the TTX concentration of Comparative Example 1.
1 and 2, in Comparative Example 1, a peak appears at a retention time of 13.28 min, so that 13.28 min is considered to be a TTX peak. However, since no TTX peak was observed in Example 1, it was found that the analysis sample did not contain TTX (non-toxic).

(Example 2)
As an extraction process, aquaculture that was removed before the development of testes and ovaries that were fed fermented fish meal, commercially available fish meal feed, raw food such as horse mackerel, mackerel, and mite, and was confirmed to be non-toxic by toxicity tests 5 kg of tiger puffer's liver was prepared.
The prepared liver was washed with water to remove blood, placed on a 60-mesh wire net approximately evenly, and locked to the upper surface of a ball-shaped pot (extraction vessel) with a capacity of 10 L. Put the kettle with the trough puffer liver on the top surface in a heating device (Steamer (manufactured by Shinagawa Kogyo Co., Ltd.), keep it at 70 ± 2 ° C and let it stand for 4 hours to extract oil from the trough puffer's liver. Thus, a liver oil mixed solution in which liver oil and water were mixed was obtained.
Next, as a separation step, the kettle was taken out of the heating device, the trough puffer liver was removed together with the wire mesh, and the liver oil mixture accumulated in the kettle was stored in a refrigerator set at 5 ° C. or lower for 24 hours.
Next, as a collecting step, it was confirmed that the oil and water in the liver oil mixture were separated, and only the liver oil on the liquid surface side was collected with a ladle.
Moreover, as a sterilization process, the obtained liver oil was filled in a nylon poly bag, sealed, and then heat-sterilized at 63 ° C. for 30 minutes in a steamer (manufactured by Shinagawa Kogyo Co., Ltd.).
The obtained liver oil was used as the liver oil of Example 2. At this time, the oil sampled on February 13, 2009 and analyzed immediately after oil extraction was taken as Example 2-1, and the oil sampled on January 30, 2010 and analyzed immediately after oil extraction was taken as Example 2-2. .

(Measurement of fatty acid composition)
In the liver oil of Example 2, analysis was requested to the Japan Food Analysis Center, and the composition of fatty acids and vitamins per 100 g was analyzed.
In addition, foie gras and anchovy liver, which is said to contain a large amount of DHA among seafood, were compared with Example 2 on the basis of the 5th revised Japanese food standard ingredient table (issued by the Ministry of Education, Culture, Sports, Science and Technology). . The composition of the fatty acid was a ratio to the total amount of fatty acid. The results are shown in Table 1. Two samples of liver oil of Example 2 were analyzed.

From Table 1, since there is almost no difference in the composition of the two specimens in Example 2, it is considered that there is no individual difference in composition, and the stability of the quality of liver oil can be obtained by using cultured trough puffer It is considered a thing.
Foie gras accounted for 98.6 mass% of the total amount of fatty acids with only saturated fatty acids and monounsaturated fatty acids, did not contain n-3 fatty acids, and the composition was significantly different from the liver of anglerfish and liver oil of Example 2. . When the liver oil of Example 2 and the liver of anglerfish are compared, the amount of saturated fatty acid is 25 mass% on average for the liver oil of example 2 and 23.1 mass% for the anglerfish liver, but there is almost no change in monounsaturated fatty acids. The difference of 51.6 mass% for anglerfish and 40.7 mass% on average for the liver oil of Example 2 was found to be about 10 mass%. Regarding polyunsaturated fatty acids, the liver of anglerfish is 23.6 mass%, the liver oil of Example 2 is about 32 mass%, and Example 2 contains more polyunsaturated fatty acids. I understood. Among the polyunsaturated fatty acids, EPA has 6.5 mass% for the angler liver and 6.2 mass% on average for the liver oil of Example 2, but there is almost no difference. Compared to 2 mass%, the average of the liver oil of Example 2 was 13.3 mass%, indicating that more DHA was contained than the liver of anglerfish.
Moreover, about vitamin A, the liver oil of Example 2-2 is contained about 1.5 times the liver of anglerfish and about 13 times the foie gras, and regarding vitamin E, the liver oil of Example 2-1 is About 2 times the liver of anglerfish, about 90 times more than foie gras, liver oil of Example 2-2 contains about 5 times more liver than anglerfish and about 220 times more than foie gras, and has a high vitamin content I understood.

  Next, Table 2 shows the contents of DHA, EPA, and vitamin E when 300 mg of the liver oil of Example 2 is sealed in a soft capsule made of porcine collagen.

In Table 2, the dietary intake standards are based on the Japanese dietary intake standards issued by the Ministry of Health, Labor and Welfare, and those 18 to 29 years old were excerpted.
From Table 2, the capsule encapsulating liver oil of Example 2 has less content of vitamin E and n-6 fatty acid than the intake target, but one intake of vitamin A is ingested for one day. I understood that I could do it. In addition, n-3 fatty acids, DHA, and EPA can be taken as 4 to 1/5 of the daily intake target by ingesting about 4 capsules, so that they can be suitably used as supplements. Guessed.

INDUSTRIAL APPLICABILITY The present invention can provide a puffer's liver oil that effectively uses the puffer's liver that has been disposed of and contains a large amount of active ingredients such as n-3 fatty acids in a balanced manner.
In addition, it is possible to efficiently extract active ingredients such as n-3 fatty acids from the puffer's liver, and to extract and sterilize liver oil without degrading and altering active ingredients such as n-3 fatty acids in liver oil. The manufacturing method of the liver oil which can be performed can be provided.

Claims (7)

  A pufferfish liver oil extracted from a pufferfish liver that has been confirmed to be non-toxic by individual toxicity tests.   The total amount of fatty acids in the liver oil contains 22-30 mass% saturated fatty acids, 35-45 mass% monounsaturated fatty acids, 27-35 mass% polyvalent fatty acids, and n- in the polyunsaturated fatty acids. 3 type fatty acid is contained 22-32 mass% with respect to the whole quantity of the said fatty acid, The puffer's liver oil of Claim 1 characterized by the above-mentioned.   The pufferfish liver oil according to claim 1 or 2, wherein the content of the n-6 fatty acid in the polyunsaturated fatty acid is 5 mass% or less based on the total amount of the fatty acid.   The pufferfish liver oil according to any one of claims 1 to 3, wherein the pufferfish is a trough pufferfish cultivated in an environment isolated from benthic organisms containing bacteria that produce pufferfish poisons.   An extraction step of extracting the oil by heating the liver of pufferfish, which has been confirmed to be non-toxic by individual toxicity tests, to 60 ° C. to 120 ° C. in the extraction container, and cooling the extraction container from which the liver has been removed The manufacturing method of the pufferfish liver oil provided with the separation process which isolate | separates the said oil component, water, etc. by this, the collection process which extract | collects the isolate | separated said oil component, and the sterilization process which heat-sterilizes the extract | collected said oil component.   The said extraction process arrange | positions the said liver in the arrangement | positioning part which has the water permeability arrange | positioned at the upper part side of the said extraction container, and heat-extracts with a steamed pufferfish oil of Claim 5 characterized by the above-mentioned. Method.   The pufferfish is a tiger puffer fish cultivated in an environment isolated from benthic organisms containing bacteria that produce puffer venom, and the liver is taken out before the development of the testes and ovaries of the pufferfish. The method for producing pufferfish liver oil according to claim 5 or 6.

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