JP5600675B2 - 未処理rca産物 - Google Patents

未処理rca産物 Download PDF

Info

Publication number
JP5600675B2
JP5600675B2 JP2011517379A JP2011517379A JP5600675B2 JP 5600675 B2 JP5600675 B2 JP 5600675B2 JP 2011517379 A JP2011517379 A JP 2011517379A JP 2011517379 A JP2011517379 A JP 2011517379A JP 5600675 B2 JP5600675 B2 JP 5600675B2
Authority
JP
Japan
Prior art keywords
rca
dna
dna polymerase
thio
product
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2011517379A
Other languages
English (en)
Japanese (ja)
Other versions
JP2011527570A (ja
JP2011527570A5 (enExample
Inventor
デイヴィス,ブライアン・エム
ネルソン,ジョン・アール
トーレス,アンドリュー・エス
ウッド,二コル・エル
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Electric Co
Original Assignee
General Electric Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Electric Co filed Critical General Electric Co
Publication of JP2011527570A publication Critical patent/JP2011527570A/ja
Publication of JP2011527570A5 publication Critical patent/JP2011527570A5/ja
Application granted granted Critical
Publication of JP5600675B2 publication Critical patent/JP5600675B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/51Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/53DNA (RNA) vaccination

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Biophysics (AREA)
  • Public Health (AREA)
  • Analytical Chemistry (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
JP2011517379A 2008-07-09 2009-06-04 未処理rca産物 Active JP5600675B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/169,993 US20100008939A1 (en) 2008-07-09 2008-07-09 Unprocessed rolling circle amplification product
US12/169,993 2008-07-09
PCT/SE2009/050667 WO2010005365A1 (en) 2008-07-09 2009-06-04 Unprocessed rolling circle amplification product

Publications (3)

Publication Number Publication Date
JP2011527570A JP2011527570A (ja) 2011-11-04
JP2011527570A5 JP2011527570A5 (enExample) 2012-05-31
JP5600675B2 true JP5600675B2 (ja) 2014-10-01

Family

ID=41505352

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2011517379A Active JP5600675B2 (ja) 2008-07-09 2009-06-04 未処理rca産物

Country Status (4)

Country Link
US (1) US20100008939A1 (enExample)
EP (1) EP2307575B1 (enExample)
JP (1) JP5600675B2 (enExample)
WO (1) WO2010005365A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110195457A1 (en) * 2010-02-09 2011-08-11 General Electric Company Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof
US9644232B2 (en) * 2013-07-26 2017-05-09 General Electric Company Method and device for collection and amplification of circulating nucleic acids
JP6080884B2 (ja) 2014-03-28 2017-02-15 富士フイルム株式会社 重合性化合物、ポリマー、重合性組成物、フィルム、および投映像表示用ハーフミラー
WO2016170182A1 (en) 2015-04-24 2016-10-27 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on a solid support
WO2016170179A1 (en) * 2015-04-24 2016-10-27 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on solid support
CN107921232A (zh) * 2015-05-18 2018-04-17 斯科特·谢弗 与补充氧气一起使用的夹式鼻腔空气加湿和鼻衄预防装置和方法
US10077459B2 (en) * 2016-05-04 2018-09-18 General Electric Company Cell-free protein expression using rolling circle amplification product
US10350307B2 (en) * 2017-09-18 2019-07-16 General Electric Company In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides
US20200392554A1 (en) * 2019-06-13 2020-12-17 General Electric Company Expression of products from nucleic acid concatemers

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5714320A (en) * 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US7030098B2 (en) * 1999-03-12 2006-04-18 The Board Of Trustees Of The Leland Stanford Junior University DNA vaccination for treatment of autoimmune disease
AU2001238535A1 (en) * 2000-02-16 2001-08-27 Large Scale Biology Corporation Rolling circle replicon expression vectors
US6323009B1 (en) * 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
CU23400A1 (es) * 2003-04-29 2009-08-04 Ct Ingenieria Genetica Biotech Método para la obtención de minicírculos replicativos de adn para transferencia génica o como inmunomoduladores
US20050202453A1 (en) * 2003-05-28 2005-09-15 Wen-Yuan Song Nucleic acid amplification in yeast
CN101103122A (zh) * 2004-12-11 2008-01-09 西托吉尼克斯公司 高质量核酸的无细胞生物合成及其用途
JP2008522628A (ja) * 2004-12-11 2008-07-03 サイトジェニックス, インコーポレイテッド 高品質核酸のセルフリー生合成及びその使用
WO2007018744A2 (en) * 2005-08-03 2007-02-15 Cytogenix, Inc. Cell-free biosynthesis of nucleic acid

Also Published As

Publication number Publication date
WO2010005365A1 (en) 2010-01-14
EP2307575B1 (en) 2017-10-11
JP2011527570A (ja) 2011-11-04
EP2307575A4 (en) 2012-09-12
EP2307575A1 (en) 2011-04-13
US20100008939A1 (en) 2010-01-14

Similar Documents

Publication Publication Date Title
JP5600675B2 (ja) 未処理rca産物
CN110418647B (zh) Rna指导的核酸修饰酶及其使用方法
KR102740888B1 (ko) 메신저 리보핵산(mRNA)을 암호화하는 안정화된 핵산
CN106434725B (zh) 小环dna载体制剂及其制备方法和使用方法
JP2024518546A (ja) 修飾されたmRNA、修飾された非コードRNA、およびその使用
JP2020535805A (ja) 細胞の遺伝子修飾のための非組込みdnaベクター
JP6918231B2 (ja) 遺伝子治療DNAベクターVTvaf17と生産方法;大腸菌株SCS110−AFと生産方法;遺伝子治療DNAベクターVTvaf17を保持する大腸菌株SCS110−AF/VTvaf17と生産方法
JP2015091236A (ja) Dnaミニサークルおよびその使用
CN103370413B (zh) Dna表达构建体
US20230212612A1 (en) Genome editing system and method
JP2024504981A (ja) 新規の操作されたヌクレアーゼおよびキメラヌクレアーゼ
CN109789185A (zh) 用于治疗眼部疾病的含cas9蛋白和向导rna的药物组合物
JP2024528060A (ja) エキソヌクレアーゼに対する抵抗性が増強された直鎖状dnaおよびその製造方法
KR20230074507A (ko) 자가-표적화 발현 벡터
CA3218780A1 (en) Methods and compositions for genomic integration
US9125845B2 (en) DNA vaccines, uses for unprocessed rolling circle amplification product and methods for making the same
JP2024533940A (ja) Ruvcドメインを有する酵素
US10350307B2 (en) In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides
FR2920158A1 (fr) Production de plasmides et expression de proteines recombinantes dans des cellules cultivees sans antibiotiques
Schakowski et al. Minimal size MIDGE vectors improve transgene expression in vivo
TW201111504A (en) Vector comprising mannose promoter and mannose promoter
JP2006526985A (ja) dsRNAを大量産生するための方法およびキット
US20160355896A1 (en) Incoated rna
JP7751885B2 (ja) ダンベル型dnaベクターを作出するための方法
KR102679172B1 (ko) 질환 세포-특이적인 miRNA에 의해 세포 생리 활성 조절 물질의 활성을 조절하는 복합체 및 이를 CRISPR/Cas 시스템에 적용한 질환 특이적 유전자 조작용 복합체

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20120327

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20120327

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130326

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20131001

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20131227

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20140722

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20140818

R150 Certificate of patent or registration of utility model

Ref document number: 5600675

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313113

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250