JP5480677B2 - Basidiomycete inhibitor and fertilizer composition for basidiomycete suppression - Google Patents

Description

  The present invention relates to a phytopathogenic agent effective for healing plant diseases and preventing the onset of plant diseases. More specifically, a phytopathogenic fungus inhibitor capable of suppressing or improving pathological fungi such as dollar spot disease, fairy ring disease, leaf rot, etc., having high safety, excellent practicality and workability, and the phytopathogenic fungus The present invention relates to a fertilizer composition containing an inhibitor and a method for producing the same.

In recent years, with increasing social interest in food safety, attempts have been made to reduce dependence on chemically synthesized drugs in agriculture.
In addition, some chemically synthesized pesticides have been reported or concerned about carcinogenicity and mutagenicity. Even in the field of vegetation management other than agriculture, there is a need to establish techniques for reducing pesticides and pesticide-free. Consideration has begun.

  However, reducing the use of chemically synthesized pesticides as they are will cause plant diseases and will not maintain the current high-quality greening environment and food production. In particular, green plants such as turfgrass play an important role in maintaining the environment of highly public facilities such as parks, schoolyards, golf courses, soccer fields, and baseball fields, and there is a strong demand for prevention of diseases.

  Examples of serious diseases of turfgrass include dollar spot disease, fairy ring disease, rhizoctonia disease, psium disease, and carbularia disease. These diseases occur in lawns with dense turfgrass, but lawns are overcrowded as plants and are often composed of a single variety, and once a disease occurs, there is a high risk of outbreaks. It is necessary to always pay attention for the control of diseases (Non-patent Document 1).

  For example, dollar spot disease has recently been regarded as a problem in Japan due to the increase in the number of onset in the summer and the emergence of the onset associated with an increase in the turfgrass planting area. In the United States, it is also an evaluation target in the National Turfgrass Evaluation Program as an important disease (Non-Patent Document 2), and it has been confirmed that it becomes a serious problem under non-agricultural management (Non-Patent Document 3). This disease not only reduces the quality of the appearance, but also greatly affects the putting quality at golf courses, so maximum efforts are made to control it.

In addition, rhizoctonia disease is one of the most important diseases that occur almost nationwide (Non-Patent Document 4), and an effective control method is desired.
Fairy Ring's disease is a disease caused by basidiomycetes, and it is said that it is extremely difficult to accurately control this disease (Non-patent Document 5).
In addition, Pythium's disease, such as pentgrass red blight, is a serious illness that can cause serious damage. It tends to suddenly occur when the humidity is high. 6) Countermeasures are desired.

  Therefore, there is a strong demand for the introduction of a plant pathogen inhibitor that is environmentally friendly, safe for the human body, low-cost, simple, and effective, but such a plant pathogen inhibitor has hardly been developed at present. .

  In view of such a situation, the present inventors pay attention to cinnamic acid which is naturally present as a safe and low environmental pollution material, and aqueous solutions and dispersions containing cinnamic acid and its derivatives, which have been used as lawn spray liquids, etc. It was clarified that the liquid substance was effective in suppressing the propagation of lawn pathogenic bacteria, and these knowledge and technical idea were disclosed as “lawn growth retention agent” (Patent Document 1). However, cinnamic acid disclosed in the document has low water solubility and cannot be made into a high concentration aqueous solution. Moreover, the target lawn pathogens are very limited.

  In agriculture and horticulture, nutrient solutions and liquid fertilizers are usually used by diluting concentrated stock solutions and diluting them appropriately when used in order to reduce labor involved in distribution and improve workability. Similarly, it is preferable to distribute the plant pathogen inhibitor as a liquid. However, the saturated aqueous solution of cinnamic acid is as low as about 0.05% at 25 ° C., and this concentration is close to the effective concentration (0.01% to 0.05%) for microbial control. There are few advantages (in the text, “%” is based on mass unless otherwise specified). In addition, since the concentration is low, there may be a problem in storage stability. In addition, solid cinnamic acid requires heating to prepare an aqueous solution, and there is a problem that the operation becomes complicated.

  In addition, due to the impact of environmental changes and the increase in turfgrass planting area, diseases such as dollar spot disease, fairy ring disease, large patch disease, and curvularia disease that have not been considered a problem in Japan have been reported in recent years. There is a need for safe and effective pathogen control agents against these pathogens.

  The above-mentioned pathogenic bacteria have greatly different properties such as chemical resistance, and even if a conventionally known agricultural chemical is applied as it is, it cannot be expected to have the same effect as another pathogenic bacteria. In addition, since the concentration of a saturated aqueous solution of cinnamic acid is about 0.05% at 25 ° C., there is a problem that diseased germs that cannot be eliminated at this concentration cannot be suppressed by cinnamic acid alone.

JP-A-5-117125

Yoshito Asano, Masahiro Kato: “Natural horticulture for 12 months, lawn” NHK Hobby, Japan Broadcast Publishing Association, 114, 2005 Masahiro Kato: “How to Read the Evaluation Table of the National Turfgrass Evaluation Program and Points of Variety Selection”, Monthly Golf Management, No. 257, pp. 70-76, 2005 Koichi Aoki: “Chapter 14 Current Status and Direction of Pesticide-Free Management”, Fumio Kitamura, Yoshisuke Kusunoki, Yoshihisa Yanagi, Akira Okubo, Yutaka Noma, Hirotosha, pp. 336-348 1997 Toshihiro Hayakawa, Mitsuro Hyakumachi: “Lisoctonia Disease”, Plant Protection, Vol. 61, No. 3, pp. 143-147, 2007 Yoshie Terashima: “Fairy Ring Disease”, Plant Protection, Vol. 61, No. 3, pp. 148-151, 2007 Kageyama Koji, Aoyagi Taketo: “Picium Disease”, Plant Protection, Vol. 61, No. 3, pp. 127-129, 2007

  The present invention has been made in view of the above circumstances, and an object of the present invention is to solve the above-mentioned problems, to be environmentally friendly, to be less toxic, and to reliably suppress pathogenic bacteria against plants such as scallops It is providing the fertilizer composition containing a pathogen control agent and this plant pathogen control agent.

The present inventor has extensively researched derivatives of cinnamic acid to achieve the above object. As a result of establishing a method for synthesizing potassium cinnamate that is not currently marketed in Japan and conducting intensive research on its properties, etc., as a result of using a mixture of cinnamic acid and an alkali metal compound or cinnamic acid salt as described above, It has been found that the problems of the prior art can be solved, and the present invention has been completed. That is, the present invention provides a mixture or basidiomycete inhibitor which comprises a cinnamate and a fertilizer composition comprising the Basidiomycetes inhibitor of cinnamic acid and an alkali metal compound.

In the basidiomycete inhibitor, the cinnamate includes at least potassium cinnamate or sodium cinnamate; the alkali metal compound is selected from the group consisting of potassium carbonate, sodium carbonate, potassium bicarbonate and sodium bicarbonate. is at least one; basidiomycete, it is a flow Eariringu disease pathogenic bacterium, the fairy ring disease pathogen, it is Chibihokoritake or Hidahokoritake; it turfgrass is creeping bentgrass, it is for turfgrass Is preferred. Moreover, this invention provides the fertilizer composition containing the said basidiomycete inhibitor .

According to the present invention, there is provided a phytopathogenic fungus inhibitor that has a higher solubility than cinnamic acid and can prepare a high-concentration aqueous solution. The plant pathogen inhibitor of the present invention contains a cinnamic acid salt, which is a natural product, as an active ingredient, has extremely high safety, and can inhibit only plant pathogens without adversely affecting the plant body. Furthermore, it can be used for healing plant diseases and maintaining plant growth. In addition, since a high concentration aqueous solution (5 to 50% as a cinnamic acid salt) impossible with cinnamic acid (maximum 0.05% concentration at 25 ° C.) can be prepared, pathogenic bacteria that could not be suppressed with cinnamic acid aqueous solution can be suppressed. In addition, it has excellent storage stability and can improve transportation efficiency. Moreover, since a high concentration solution can be prepared, it can respond also to the use of a stock solution dilution system.
By using the phytopathogenic fungus inhibitor of the present invention, it is possible to improve the efficiency of vegetation management in parks, schoolyards, green spaces, golf courses, soccer fields, etc., and to improve the productivity of grains, grass and feed.

The fluorescent-X-ray-analysis chart of white powder (A). Infrared absorption spectrum of cinnamic acid and white powder (A). Ion chromatogram of white powder (A). The graph which shows the effect with respect to dollar spot disease. The graph which shows the effect with respect to dollar spot disease. The graph which shows the effect with respect to dollar spot disease.

Next, the present invention will be described in more detail with reference to the best mode for carrying out the invention. Cinnamic acid salt used as a phytopathogenic agent inhibitor in the present invention is an alkali metal salt or alkaline earth metal salt of cinnamic acid. More specific examples include potassium cinnamate and sodium cinnamate.
The concentration of a saturated aqueous solution of cinnamic acid is about 0.05% at 25 ° C., but an alkali metal salt of cinnamic acid, particularly potassium cinnamate, can easily prepare a high concentration aqueous solution (for example, 20%). . For this reason, not only can a high concentration solution excellent in storage stability be prepared, but also the transport efficiency is excellent. In addition, taking advantage of its high water solubility, it can be transported as powders and granules, and can easily be prepared at the application site to prepare the phytopathogenic fungus inhibitor of the present invention at a concentration according to the application purpose. Can also contribute to

  Cinnamic acid is a C6-C3 compound (phenylpropane derivative), and is a precursor in the biosynthesis process of plant components such as lignin, lignan, catechin, flavonoid, anthocyanin, stilbene, and chalcone, and therefore has good compatibility with plants. Cinnamic acid has the same structure as the degradation product of lignin, and even if it is disposed of in the environment, it is microbially decomposed and can become a humic material. High safety. In addition, cinnamate has a large LD50 value (for example, in the case of sodium cinnamate, it is 2 g / kg by intraperitoneal administration in mice), and no mutagenicity has been reported. Very little toxicity to plants such as plants. On the other hand, since the microbial inhibitory effect, particularly the inhibitory effect on fungi (fungi), is remarkable, the use of cinnamate can suppress phytopathogenic bacteria without damaging the plant body. . From the above, the plant pathogen inhibitor of the present invention is not only effective in suppressing and preventing microorganisms, particularly plant diseases originating from fungi, but also effective in maintaining environmental sanitation around the plant body.

  The plant pathogen inhibitor of the present invention may contain a mixture of cinnamic acid and an alkali metal compound instead of using cinnamic acid salt. Examples of alkali metal compounds include potassium carbonate, sodium carbonate, potassium bicarbonate, sodium bicarbonate, potassium nitrate, sodium tripolyphosphate, potassium tripolyphosphate, sodium polyphosphate, potassium polyphosphate, trisodium phosphate, tripotassium phosphate, Examples include dipotassium hydrogen phosphate, disodium hydrogen phosphate, sodium acetate, and potassium acetate. Among these alkali metal compounds, those that can be suitably used include at least one selected from the group consisting of potassium carbonate, sodium carbonate, potassium bicarbonate and sodium bicarbonate.

  There is no limitation on the plant subject to application of the plant pathogen inhibitor of the present invention and the fertilizer composition containing the plant pathogen inhibitor, and any plant can be expected to recover the disease state and maintain the plant growth by suppressing the plant pathogen. A suitable plant to be applied is a plant of this family. Further, the plant pathogen inhibitor of the present invention and a fertilizer composition containing the plant pathogen inhibitor include greening plants such as turfgrass, cereals such as rice and wheat, orchardgrass, and timothy It is particularly preferably used for grass and feed.

  Examples of turfgrass to which the phytopathogenic agent of the present invention can be suitably applied include both warm and turfgrass turfgrass. Examples of the warm-type turfgrass include Himekouraishiba, Koraishiba, Noshiba, Bermudagrass, St. Augustinegrass, buffalograss, centipedegrass, carpetgrass, bahiagrass, etc., but particularly effective for Bermudagrass. In addition, Kentucky Bluegrass, Tall Fescue, Creeping Red Fescue, Chewing Fescue, Hard Fescue, Perennial Ryegrass, Italian Ryegrass, Creeping Ventgrass, Colonial Ventgrass, etc., are effective for creeping bentgrass. Is big.

  Examples of cereals to which the phytopathogenic agent of the present invention can be suitably applied include rice, barley, wheat, rye, oat, pearl barley, millet, millet, millet, corn, sorghum, sugar beet, macomo, etc. Great effect on wheat.

  Examples of the grass and feed to which the phytopathogenic agent of the present invention can be suitably applied include both warm and cold grasses. Examples of warm grasses include rosegrass, bahiagrass, dalithgrass, guineagrass, colored guineagrass, napiergrass, pangolagrass, giant stargrass, bermudagrass, and chrysanthemumgrass. In cold region type grass, there are orchard grass, timothy, italian ryegrass, perennial ryegrass, tall fescue, meadow fescue, smooth bromegrass, etc., but it is highly effective against orchard grass and timothy.

  By suppressing phytopathogenic fungi using the phytopathogenic fungus inhibitor of the present invention and a fertilizer composition containing the phytopathogenic fungi inhibitor, plant diseases can be cured or prevented, and plant growth can be maintained. . Examples of plant diseases to be cured or prevented include fungal diseases and bacterial diseases, and are particularly effective against fungal diseases.

Examples of the fungal diseases that can be suitably suppressed by the plant pathogen inhibitor of the present invention include dollar spot disease, rhizobonia disease, psium disease, carbraria disease, fairy ring disease and the like. Here, dollar spot disease is a disease of turfgrass, caused by a baby bacilli having the scientific name of Sclerotinia homoeocarpa, and includes a disease called dog footprint.
Rhizoctonia disease is a disease caused by Rhizoctonia, which is an incomplete bacterium, and causes various symptoms such as seedling wilt and root rot for various crops. Rhizoctonia diseases include seedling blight, root rot, rice bran disease, and spider web disease. For rhizobonia disease against turfgrass, leaf rot, brown leaf rot, spider web disease, prawn patch, coat blight, large patch, lysoctonia large patch whose pathogenic fungus has the scientific name Rhizoctonia solani Diseases such as pseudo leaf rot, yellow patch, winter patch, elephant footprint, spring baldness, rhizoctonia spring baldness, white patch, winter leaf rot, etc. where binucleate Rhizoctonia is the pathogen Illness called.

Pisium disease is a disease caused by the genus Pythium, and causes various symptoms such as seedling wilt, seed rot, and root rot for various crops. Picium disease for turfgrass includes diseases such as pisium spring baldness, psium patch, psium bright, irregularity, cotton rot, and red burn.
In addition, the carbaria disease is a disease caused by an incomplete fungus, Calvularia geniculata, and includes diseases such as turfgrass blight, leaf blight, carbaria leaf blight.

  Fairy-Ring's disease refers to Lycoperdon perlatum, Bobista dermoxantha, Lycoperdon pusillum, Vascellum curtisii, Asp. The turfgrass disease caused by the fungus causes the symptom that the turfgrass dies in a ring shape, a dark green ring is formed on the turfgrass, and the fruit body of a mushroom is generated in a ring shape.

Conventional fungicides for plants used in agriculture, forestry, horticulture, etc. have been reported to have genotoxicity such as mutagenicity, tumorigenicity, or carcinogenicity, or have high persistence and are suspected of environmental hormones. Some have
However, the phytopathogenic agent suppressor of the present invention contains cinnamic acid salt, which is a substance naturally present in plants, as an active ingredient, mutagenicity and endocrine found in fungicides mainly composed of chemically synthesized substances. There is no suspicion of disturbance. In addition, since the residual property is low, it can be used very safely. The plant pathogen inhibitor of the present invention does not have serious toxicity to plants, can suppress only plant pathogens, and can be used for healing plant diseases and maintaining plant growth. As described above, it has an excellent inhibitory effect on plant diseases caused by fungi in particular.

  In addition, cinnamic acid salt used in the phytopathogenic fungus inhibitor of the present invention has higher water solubility than cinnamic acid, and by preparing and spraying aqueous solutions of various concentrations of cinnamic acid, it can also be used for plant body prevention. It can be effective for treatment. Specifically, it is sprayed directly on the affected part of the plant body or sprayed around the plant body. For example, the aqueous solution is sprayed on the roots, stems, leaves, etc. of a diseased plant, the roots, stems, leaves, etc. of the diseased plant are immersed in the aqueous solution, or on the soil around the plant. By spraying an aqueous solution, it can be used as a phytopathogenic agent inhibitor.

When using the plant pathogenic fungus inhibitor of the present invention, it is transported to the application site as a powder or granules, and an aqueous solution having a concentration suitable for the purpose at the site of use (for example, 0.5% potassium cinnamate) is used. It is preferred to prepare and apply immediately. Further, a solution containing cinnamate may be prepared as a concentrated solution (stock solution) and appropriately diluted depending on the purpose at the time of use. Further, if necessary, it can also be used by mixing with a spreading agent, other antibacterial agents, bactericides, other additives and the like. The cinnamate can be used as an inclusion compound with cyclodextrin or the like, or supported on a carrier such as zeolite or silica and used as a powder or a suspension thereof.
In addition to the use as an aqueous solution, an emulsion, dispersion, or suspension may be prepared and used using the aqueous solution of the phytopathogenic fungus inhibitor of the present invention. Alternatively, it may be sprayed as a powder or granular solid and then sprayed with an appropriate amount of water.

When the phytopathogenic fungus inhibitor of the present invention is used as an aqueous solution, it is used as an aqueous solution of about 0.01 to 2.0% as cinnamate as a temporary standard. Moreover, when it is set as a concentrate for the improvement of the convenience of distribution | circulation, as an example, it can prepare to about 0.1 to 30% as a cinnamate.
The amount of the phytopathogenic agent inhibitor of the present invention is not particularly limited, but when potassium cinnamate is used, the amount of 0.1 to 6.0 g of potassium cinnamate per unit area (m ² ) of the application area is 2 It is effective to spread ~ 4 times / month. If the amount used is too large, the growth of the plant body may be adversely affected, and if it is too small, the sufficient effect may not be exhibited. Even when other salts are used, an amount according to potassium cinnamate (0.1 to 5.0 g as a cinnamic acid residue part) is sprayed in the same manner as described above.

  Moreover, by mixing the plant pathogen inhibitor of the present invention with various agricultural / horticultural materials, chemicals such as agricultural chemicals, the plant pathogen inhibitory action can be imparted to them. Examples of agricultural and horticultural materials include surfactants, colorants, fragrances, plant nutrients, and various additives. Examples of agricultural chemicals include other antibacterial agents, fungicides, herbicides, insecticides, spreading agents, plant growth regulators, and repellents. Since the plant pathogen inhibitor of the present invention is characterized by being environmentally friendly, it is preferably mixed with a natural product-based drug having a low environmental impact.

  Moreover, the plant pathogen control effect | action can be provided to these fertilizers by mixing the plant pathogen inhibitor of this invention with various fertilizers. It can be used as a fast-acting phytopathogenic fungus inhibitor by mixing with a fertilizer component having particularly high water solubility and applying the fertilizer composition as an aqueous solution. By using such a fast-acting phytopathogenic fungus inhibitor, the phytopathogenic fungi can be controlled and cured in an effective, convenient and safe manner at an appropriate time of plant management, for example, watering.

  The fertilizer mixed with the phytopathogenic fungus inhibitor of the present invention only needs to contain fertilizer components such as nitrogen, phosphoric acid, potassium, calcium, magnesium, manganese, and boron. Examples of fertilizers suitable for mixing with the phytopathogenic agent of the present invention include nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, calcareous fertilizer, siliceous fertilizer, bitter soil fertilizer, manganese fertilizer, boron fertilizer, Compound fertilizer (molten compound fertilizer, chemical fertilizer, molded compound fertilizer, adsorption compound fertilizer, coated compound fertilizer, byproduct compound fertilizer, liquid compound fertilizer, compound fertilizer, compound fertilizer for home gardening), trace element compound fertilizer, pesticide and others Common fertilizers such as fertilizers mixed with things, organic fertilizers and sludge fertilizers can be mentioned.

  Of the above, examples of nitrogenous fertilizers include ammonium sulfate (ammonium sulfate), ammonium chloride (ammonium salt), ammonium sulfate, ammonium humate, ammonium nitrate (ammonium nitrate), ammonium nitrate lime (ammonium nitrate), lime nitrate, nitrate Soda, urea, coated urea, lime nitrogen, IB (isobutylidene 2 urea, isobutyraldehyde processed urea fertilizer), CDU (crotonylidene 2 urea, acetaldehyde processed urea fertilizer), ureaform (uraform, form nitrogen, formaldehyde processed urea fertilizer), GU ( In addition to inorganic fertilizers such as guanylurea and oxamide, organic fertilizers such as oil cakes and fish manures can be mentioned.

  Examples of phosphate fertilizers include superphosphate lime, heavy superphosphate lime, bitter clay superphosphate, molten phosphorus fertilizer (molten phosphorus), BM molten phosphorus, calcined phosphorus fertilizer, molten phosphorus, Organic fertilizers such as bone meal and rice bran can be mentioned in addition to inorganic fertilizers such as heavy burnt phosphorus, hard clay burnt phosphorus, humic acid mixed phosphorus fertilizer and byproduct phosphorus fertilizer.

  Examples of calcareous fertilizers include potassium chloride, potassium sulfate, potassium sulfate bitter clay, potassium bicarbonate, potassium silicate, and humic acid potassium. Examples of calcareous fertilizers include quick lime, slaked lime, calcium carbonate, Examples include lime, mixed lime fertilizer, and shell fossil fertilizer.

  Examples of water-soluble fertilizer components suitable for the fertilizer composition containing the plant pathogen inhibitor of the present invention include ammonium sulfate, ammonium chloride, ammonium nitrate, sodium nitrate, calcium nitrate, urea, phosphoric acid, and monoammonium phosphate. , Diammonium phosphate, lime superphosphate, potassium chloride, potassium sulfate, 1 potassium phosphate, 2 potassium phosphate, and potassium nitrate.

When producing an alkali metal salt as cinnamic acid salt, which is a phytopathogenic fungus inhibitor of the present invention, by gradually dissolving cinnamic acid while maintaining the alkalinity of the aqueous solution in the presence of a salt or base whose aqueous solution exhibits alkalinity, An aqueous solution of cinnamic acid is prepared, and high-purity cinnamic acid salt can be obtained from this solution by crystallization.
Potassium cinnamate, which is one of the most preferred cinnamates used as the plant pathogen inhibitor of the present invention, is not currently marketed in Japan and cannot be easily obtained.

  The method for obtaining potassium cinnamate is shown below. First, in about 90 ° C. hot water, 30% cinnamic acid and 13% potassium hydroxide (purity 85%, reagent grade) are each gradually divided into 3 times each in 1/3 amounts. An aqueous solution having a cinnamic acid concentration of about 30% is prepared. The pH at this time is around 7.8. Next, this aqueous solution is cooled to 4 ° C. and left at this temperature for 24 hours. A white precipitate is formed, which is separated by filtration. The white precipitate thus filtered is dried at 105 ° C. for about 6 hours, pulverized, and cinnamon. A white powder of potassium acid can be obtained. Alternatively, the cinnamic acid solution is spread thinly on a vat and heated at 105 ° C. for about 6 hours to evaporate and dry the water, and then the resulting white solid is pulverized to obtain a white powder of potassium cinnamate. Although the usage-amount of the salt or base used in the case of said cinnamic acid aqueous solution preparation is not specifically limited, the quantity used as 30-300% with respect to a cinnamic acid is preferable.

  On the other hand, commercially available sodium cinnamate can be obtained. When synthesizing sodium cinnamate, first, 1% each of cinnamic acid in an amount of 10% and sodium hydroxide in an amount of 2.6% (purity 97%, reagent grade) in hot water at about 90 ° C. The solution is gradually dissolved in 3 portions in 3 portions to prepare an aqueous solution having a cinnamic acid concentration of about 10%. The pH at this time is around 7.7. Next, this aqueous solution is cooled to 4 ° C. and left at this temperature for 24 hours. A white precipitate is formed, which is separated by filtration. The white precipitate thus filtered is dried at 105 ° C. for about 6 hours, pulverized, and cinnamon. A white powder of sodium acid can be obtained. Alternatively, the cinnamic acid solution is spread thinly on a vat and heated at 105 ° C. for about 6 hours to evaporate and dry the water, and then the resulting white solid is pulverized to obtain a white powder of sodium cinnamate. Although the usage-amount of the salt or base used in the case of said cinnamic acid aqueous solution preparation is not specifically limited, the quantity used as 20-200% with respect to a cinnamic acid is preferable.

  Any base or salt used in preparing cinnamic acid salt, which is an active ingredient of the phytopathogenic agent of the present invention, can be used as long as the base or its aqueous solution is a salt of a weak acid and a strong base that are alkaline. However, it is particularly preferable to use a material that exhibits pH buffering action, has a low risk of environmental pollution, and is safe for the human body. Examples of the base include sodium hydroxide and potassium hydroxide. Examples of the salt of a weak acid and a strong base include potassium bicarbonate, sodium bicarbonate, sodium tripolyphosphate, potassium tripolyphosphate, sodium polyphosphate, and polyphosphoric acid. Preferred examples include potassium, trisodium phosphate, tripotassium phosphate, dipotassium hydrogen phosphate, disodium hydrogen phosphate, potassium carbonate, sodium carbonate sodium acetate, and potassium acetate. Particularly preferred bases or salts include potassium hydroxide, potassium bicarbonate, sodium bicarbonate, sodium tripolyphosphate, potassium carbonate and sodium acetate which are also used in foods.

Next, the present invention will be described more specifically with reference to examples. In the text, “part” or “%” is based on mass.
<Preparation of potassium cinnamate powder>
(1) Preparation of white powder (A) 130 g of potassium hydroxide (purity 85%, reagent grade) was dissolved in 650 mL of 90 ° C. distilled water, and 302 g of cinnamic acid was gradually added to this potassium hydroxide solution and stirred. After dissolution, the solution was diluted to 1000 mL with distilled water to prepare a cinnamic acid aqueous solution (pH 7.8) having a concentration of 30%. Next, this solution was cooled to 25 ° C. and then allowed to stand at 4 ° C. for 12 hours to precipitate a white substance. The white substance was taken out, air-dried, and dried at 105 ° C. for 6 hours to obtain 370 g of a white powder. Hereinafter, the white powder is referred to as white powder (A).

(2) Confirmation of potassium cinnamate a) Confirmation of potassium When the white powder (A) was subjected to fluorescent X-ray analysis, the chart shown in FIG. 1 was obtained. When this chart was seen, the peak of potassium was confirmed in the vicinity of 140 2θ (°) (Kα: indicated by KA in FIG. 1), and it became clear that the potassium component was present in the white powder (A).

b) Confirmation of carboxylate formation When the white powder (A) was analyzed by infrared spectroscopy, an infrared absorption spectrum shown on the lower side of FIG. 2 was obtained. Comparing this spectrum with the infrared absorption spectrum of cinnamic acid (upper side of FIG. 2), in the spectrum of white powder (A), the free carboxylic acid peak near 1700 cm ⁻¹ seen in the cinnamic acid spectrum disappears, A new carboxylate peak was observed near 1550 cm ⁻¹ . This revealed that a salt of carboxylic acid was present in the white powder (A).

c) Quantitative determination of potassium component 1 g of white powder (A) was accurately weighed and placed in a 200 mL volumetric flask, 50 mL of purified water and 5 mL of 0.2 M nitric acid were added, and the mixture was shaken to 200 mL with purified water. Further, when the sample was diluted 20 times with purified water and analyzed by ion chromatography, the chromatogram shown in FIG. 3 was obtained. The amount of potassium calculated from the peak area of potassium in this chromatogram was 0.21 g. From this result, it was revealed that the potassium component in the white powder (A) was 21.0%.

d) Determination of cinnamic acid component Accurately weigh 0.05 g of chloroform-treated white powder (A) into a 50 mL volumetric flask, add 1 mL of 0.5 M hydrochloric acid and 20 mL of purified water, shake and mix, and add ethanol to 50 mL. It was. Further, the cinnamic acid component in the white powder (A) was quantified by comparison with a standard cinnamic acid solution (control) using an absorptiometric method (270 nm), and the cinnamic acid component It was found to be 76.7% in the white powder.

e) Confirmation of content of white powder (A) From the results of the analytical tests of a) to d) above, it was confirmed that white powder (A) was potassium cinnamate and its pure content was about 98%. .

<Inhibition test of potassium cinnamate aqueous solution against pathogenic bacteria of dollar spot disease>
[ Reference Example 1]
Using an aqueous solution of the above white powder (A), a potato dextrose agar medium (PDA medium: Eiken Equipment Co., Ltd.) having a potassium cinnamate concentration of 0.01%, 1 g of potato leachate, 20 g of glucose, and 15 g of agar Were prepared and dispensed into a petri dish having a diameter of 90 mm to prepare an agar plate. The center of this plate was inoculated with one platinum loop of Mylar of spotler pathogen (Sclerotinia homoeocarpa), cultured at 25 ° C., and the diameter of the colony was measured after 2 days, 5 days and 8 days from the culture. The results are shown in Table 1.

[ Reference Example 2]
Colonies were measured in the same manner as in Reference Example 1 except that the concentration of potassium cinnamate in the PDA medium was 0.05%. The results are shown in Table 1. As a result of the measurement, it was confirmed that the effect of suppressing the growth of pathogenic bacteria was more remarkable than that in Reference Example 1.

[ Reference Example 3]
Colonies were measured in the same manner as in Reference Example 1 except that the concentration of potassium cinnamate in the PDA medium was 0.1%. The results are shown in Table 1. In Reference Example 3, the generation of colonies was not confirmed until the 8th day.

[Comparative Example 1]
Colonies were measured in the same manner as in Reference Example 1 except that potassium cinnamate was not added to the PDA medium. In Comparative Example 1, colonies grew on the entire plate surface after 5 days from the start of culture.
From the above results, it became clear that the aqueous solution of potassium cinnamate according to the present invention can effectively suppress the growth of the disease spot pathogen which is a lawn bacterium.

<Inhibition test of potassium cinnamate against isolates of dollar spot disease lesions>
[ Reference Examples 4 to 6, Comparative Example 2]
(1) Isolation of pathogenic fungi from dollar spot disease lesions Two dominant strains of fungal growth were isolated from turfgrass exhibiting disease conditions of dollar spot disease by a plate dilution method using a fungal medium. And isolate B.

(2) Inhibition test of pathogenic bacteria from dollar spot disease lesions The diameters of the colonies were measured in the same manner as in Reference Examples 1 to 3 and Comparative Example 1 except that the isolates A and B were used as the pathogens of dollar spot disease. ( Reference Examples 4 to 6, Comparative Example 2). The results are shown in Table 2. From the results in Table 2, it was revealed that an aqueous solution of potassium cinnamate, which is a phytopathogenic fungus inhibitor of the present invention, can effectively suppress the growth of dollar spot lesion focus isolates. In particular, when the concentration of potassium cinnamate in the medium exceeded 0.01%, no colonies were formed on the plate, and the generation of dollar spot lesion focus isolates could be completely suppressed.

<Disease reduction effect against dollar spot disease in bentgrass>
[ Reference Example 7]
In 400 mL of distilled water at 20 ° C., 2.0 g of the white powder (A) was dissolved to prepare a 0.5% potassium cinnamate aqueous solution.
A test plot (1 test plot area of 1 m ² ) was set in the turfgrass (creeping bentgrass) field where the dollar spot disease occurred, and the above 0.5% potassium cinnamate aqueous solution (0.5% plot) was applied. . The application rate was 250 mL / m ² at one time. The number of applications was 4, the first application date was day 0, the second application was on day 9, the third application was on day 16, and the fourth application was on day 23. On the first application day (before application, day 0), the 15th day and the 26th day, the number of lesion spots (number of lesions per 0.25 m ² ) of each spot was measured, and the severity of the disease ( 0: None-4: 甚) was evaluated in 0.1 units, and 3 test sections were measured and evaluated. The transition of the average value is shown by dotted lines in FIGS.

[ Reference Example 8]
The number of lesions was measured in the same manner as in Reference Example 7 except that the amount of white powder (A) used was 4.0 g and a 1% strength aqueous potassium cinnamate solution was used, and the degree of disease was evaluated. The transition of the average value in the three test sections is shown by broken lines in FIGS.

[Comparative Example 3]
The number of lesions was measured in the same manner as in Reference Example 7 except that the white powder (A) was not used, and the degree of disease was evaluated. The transition of the average value of the three test plots is shown by solid lines in FIGS.
From these results, in the area where the potassium cinnamate aqueous solution was applied, there was a tendency that both the number of spot spots and the severity of the disease were reduced and reduced, and the phytopathogenic fungus inhibitor of the present invention can reduce the pathology of dollar spot disease. Became clear.

<Control effect against dollar spot disease in bentgrass>
[ Reference Example 9]
0.5 g of the white powder (A) was dissolved in 400 mL of distilled water at 20 ° C. to prepare a potassium cinnamate aqueous solution having a concentration of 0.125%.
A test plot (the width of one test plot was 1 m ² ) was set in a turfgrass (creeping bentgrass) field in which dollar spot disease occurred, and the above 0.125% potassium cinnamate aqueous solution was applied. The application rate was 250 mL / m ² at one time. The number of applications is 5 times, the first application date is day 0, the second application is the seventh day, the third application is the 13th day, the fourth application is the 25th day, and the fifth application is 29. I went on the day. On the first application day (before application, day 0), 7, 13, 25, 29, and 36 days, the number of spots of dollar spot disease in each ward (per 0.25 m ² ) The number of lesions) was measured. The transition of the average value in the three test zones is shown by the dotted line in FIG.

[ Reference Example 10]
The number of lesions was measured in the same manner as in Reference Example 9 except that the amount of white powder (A) used was 1 g and a 0.25% potassium cinnamate aqueous solution was used. The transition of the average value of the three test sections is shown by a one-dot chain line in FIG.

[ Reference Example 11]
The number of lesions was measured in the same manner as in Reference Example 9 except that the amount of white powder (A) used was 2 g and a 0.5% strength aqueous potassium cinnamate solution was used. The transition of the average value in the three test sections is shown by a broken line in FIG.

[Comparative Example 4]
The number of lesions was measured in the same manner as in Reference Example 9 except that the white powder (A) was not used. The transition of the average value in the three test zones is shown by a solid line in FIG.
From these results, it becomes clear that the application of the potassium cinnamate aqueous solution can suppress the increase in the number of spots of dollar spot disease, and that the application of the plant pathogen inhibitor of the present invention can effectively suppress the dollar spot disease. It was.

<Inhibition test of potassium cinnamate aqueous solution against fairy ring disease pathogenic fungus Cibichocorchus>
[Example 12]
Prepare an agar plate with a potassium cinnamate concentration of 0.01% in the same procedure as in Reference Example 1, and inoculate the center of the plate with one platinum loop of fairy ring disease pathogenic fungus Bovista dermoxantha (Lp41) And cultured at 25 ° C. Colony diameters were measured after 4 days, 8 days, 12 days, and 25 days after culturing. The results are shown in Table 3.

[Examples 13 and 14, Comparative Example 5]
The same experiment as in Example 12 was performed except that the potassium cinnamate concentration in the agar plate was 0.05%, 0.1% and 0%. The results are shown in Table 3, respectively.
When potassium cinnamate was contained in the plate, the occurrence of colonies could not be confirmed at any concentration of 0.01% to 0.1%. On the other hand, in Comparative Example 5 in which potassium cinnamate was not added, colonies were generated and grew with time.
From these results, it was clarified that the growth of the fairy ring disease pathogenic fungus Cibichocorch can be effectively suppressed by adding 0.01% or more of potassium cinnamate to the medium.

<Control effect against fairy ring disease pathogenic fungus Chibidokoritake>
[Example 15]
(1) Turfgrass Medium A mixture of 9 parts of bentgrass as turfgrass and 9 parts of Metromix 350 (manufactured by Sunglo Inc., Canada) was prepared, and a medium with 7.5 parts of water added thereto was prepared. It was.

(2) Preculture and inoculation source The above-mentioned turfgrass medium was filled into a biopot (diameter 8 cm, height 13 cm) to a height of about 5 cm, and this was inoculated with the fairy ring disease pathogen Bovista dermoxantha (Lp41). This biopot was cultured at 28 ° C. for 14 days to prepare an inoculum.

(3) Inoculation and spraying with aqueous potassium cinnamate solution Prepare 10 other biopots filled with turfgrass medium, and insert about 2/3 of the plant culture bottle (diameter 2.5 cm, height 10 cm) into the center of each. Holes were secured with and these biopots were autoclaved. After sterilization, the culture bottle is taken out, and two cups of the above inoculum are put into the resulting hole with a medicinal pot, the turfgrass medium is covered with about 2 cm, and the cinnamate potassium prepared using the white powder (A) from above. 4 mL of 1% aqueous solution was sprayed per pot.

(4) Effectiveness measurement These biopots were cultured at 28 ° C., and the mycelium area on the top of the soil was measured on the 28th day from the start of the culture. (%) Was calculated by the following formula.
Surface area ratio (%) without fungus growth = [(surface area cm ² without fungus growth) / (pot surface area cm ² )] × 100
Ratio of number of pots without bacterial growth (%) = [(Number of pots without bacterial growth) / Total number of pots] × 100
A very small amount of mycelium was generated in 4 of 10 pots, but all were insufficient for measuring the mycelial area. A summary of the results is shown in Table 4.

[Comparative Example 6]
The experiment was performed in the same procedure as in Example 15 except that a commercially available agrochemical for controlling turf disease (commercial product A) diluted 2000 times was used instead of the 1% aqueous solution of potassium cinnamate. Generation of mycelium was confirmed in all pots 28 days after the start of the culture. A summary of the results is shown in Table 4.

[Comparative Example 7]
The experiment was conducted in the same procedure as in Example 15 except that distilled water was used instead of the 1% aqueous solution of potassium cinnamate. After 28 days from the start of culture, the mycelium covered the surface in all pots. A summary of the results is shown in Table 4.

(5) Judgment of the effect From these results, the pot sprayed with the potassium cinnamate aqueous solution of the present invention has a large number of pots without bacterial growth. It became clear that it could be controlled.

<Inhibition test against fairy leaf disease pathogen Hida-Hokoritake>
[Examples 16 to 18, Comparative Example 8]
Except that the measurement of the diameter of the colony was performed 4, 8, 12, and 25 days after the start of culture using the fairy ring disease pathogen Vascellum curtisii (Vp19) instead of the dollar spot disease pathogen (Sclerotinia homoeocarpa) Experiments were performed in the same procedure as in Reference Examples 1 to 3 and Comparative Example 1. As a result, in the medium containing potassium cinnamate according to the present invention, no colander of the octopus mushroom was generated even after 25 days from the start of the culture. A summary of the results is shown in Table 5.
From these results, it became clear that the growth of octopus mushrooms can be effectively suppressed by adding 0.01% or more of potassium cinnamate to the medium.

<Control effect against fairy leaf disease pathogen Hida Hokoritake>
[Example 19]
(1) Turfgrass medium In the same manner as in Example 15 except that the fairy ring disease pathogen Vascellum curtisii (Vp19) was used instead of the fairy ring disease pathogen Bovista dermoxantha (Lp41). The body surface area was measured.
As a result, the surface area ratio without growth of bacteria 28 days after the start of culture was 78%.

[Comparative Example 9]
An experiment similar to that of Example 19 was performed except that sterilized water was used instead of the 1% aqueous solution of potassium cinnamate. The surface area ratio without bacterial growth after 18 days from the start of the culture was 18%.
The pot sprayed with the aqueous potassium cinnamate solution of the present invention had a surface area ratio without growth of bacteria of 4 times or more as compared with the pot of the comparative example sprayed with sterilized water. From these results, it was revealed that the phytopathogenic fungus inhibitor of the present invention can effectively control the fairy leaf disease pathogenic fungus Mushroom.

<Suppression test against causalaria disease pathogen>
[ Reference Examples 20 to 22, Comparative Example 10]
Reference Examples 1 to 3 and Comparative Example, except that Curvularia geniculata was used instead of Sclerotinia homoeocarpa and colony diameter was measured 2, 4, and 7 days after the start of culture. The experiment was performed in the same procedure as in Example 1 ( Reference Examples 20 to 22 and Comparative Example 10). The results are shown in Table 7.
Even when the concentration of potassium cinnamate in the medium is 0.01%, the growth of the pathogenic bacteria of curraria disease can be suppressed, and at 0.05% and 0.1%, colonies do not occur until 7 days after the start of culture, It was possible to completely inhibit the growth of pathogenic bacteria. From these results, it was shown that potassium cinnamate, which is a phytopathogenic agent inhibitor of the present invention, can effectively inhibit the growth of the causal fungus pathogen.

<Inhibition test of potassium cinnamate against large patch disease pathogen>
[ Reference Example 23]
The concentration of potassium cinnamate in the PDA medium was 0.2%, and a large patch disease pathogen (Rhizoctonia solani AG2-2 LP) was used instead of the dollar spot disease pathogen, and the colony diameter was measured from the beginning of culture to 3, 7, 13 The experiment was performed in the same manner as in Reference Example 1 except that the test was conducted after a day. As a result, even after 13 days from the start of the culture, no colonies were generated on the plate.

[ Reference Example 24]
The same experiment as in Reference Example 23 was performed except that the concentration of potassium cinnamate in the PDA medium was 0.5%. As a result, even after 13 days from the start of the culture, no colonies were generated on the plate.
[Comparative Example 11]
An experiment similar to Reference Example 23 was performed except that cinnamic acid was used instead of potassium cinnamate and the cinnamic acid concentration in the PDA medium was 0.05%. Three days after the start of culture, colonies appeared on the plate, and after 13 days, colonies grew until the entire surface of the plate was covered.
[Comparative Example 12]
An experiment similar to Reference Example 23 was performed except that a PDA medium was prepared using distilled water instead of the potassium cinnamate aqueous solution. Colonies grew rapidly after the start of culturing, and after 7 days, colonies grew until the entire surface of the plate was covered.

Table 8 summarizes the results of Reference Examples 23 and 24 and Comparative Examples 11 and 12. From these results, it was shown that the potassium cinnamate aqueous solution, which is a plant pathogen inhibitor of the present invention, can effectively suppress the growth of large patch disease pathogens. The plant pathogenic fungus inhibitor of the present invention is higher in water solubility than cinnamic acid, so that a solution having a higher concentration than a saturated cinnamic acid aqueous solution can be prepared. By using such a high concentration solution, cinnamic acid As compared with the case of using a saturated aqueous solution, it was found that it can exert a much stronger effect of inhibiting large patch disease pathogens.

<Inhibition test of potassium cinnamate aqueous solution against brown patch disease pathogen>
[ Reference Example 25]
The experiment was conducted in the same manner as in Reference Example 24 except that a brown patch disease pathogen (Rhizoctonia solani AG2-2IIIB) was used instead of the large patch disease pathogen. As a result, even after 13 days from the start of the culture, no colonies were generated on the plate.

[Comparative Example 13]
An experiment similar to Reference Example 25 was performed except that cinnamic acid was used in place of potassium cinnamate and the cinnamic acid concentration in the PDA medium was 0.05%. As a result, colonies grew from the start of the culture, and grown 13 days after the start of the culture so that the colonies covered the entire plate surface.

[Comparative Example 14]
An experiment similar to Reference Example 25 was conducted, except that a PDA medium was prepared using distilled water instead of the potassium cinnamate aqueous solution. As a result, colonies grew rapidly from the start of the culture, and grown 7 days after the start of the culture so that the colonies covered the entire plate surface.
A summary of Reference Example 25 and Comparative Examples 13 and 14 is shown in Table 9. From the result of Table 9, it was shown that the growth of Brown patch disease pathogen can be effectively suppressed by containing potassium cinnamate which is a plant pathogen inhibitor of the present invention in the medium. The plant pathogenic fungus inhibitor of the present invention is higher in water solubility than cinnamic acid, so that a solution having a higher concentration than a saturated cinnamic acid aqueous solution can be prepared. By using such a high concentration solution, cinnamic acid As compared with the case of using a saturated aqueous solution, it was found that it can exert a much stronger brown patch disease pathogen control effect.

<Inhibition test of potassium cinnamate against carnation root rot pathogen>
[ Reference Example 26]
Similar to Reference Example 25, except that Pythium aphanidermatum was used instead of the brown patch disease pathogen and the diameter of the colony was measured 3, 7, and 15 days after the start of culture. The experiment was conducted. As a result, no colonies were generated on the plate even after 15 days from the start of the culture.

[Comparative Example 15]
An experiment similar to Reference Example 26 was performed except that cinnamic acid was used instead of potassium cinnamate and the cinnamic acid concentration in the PDA medium was 0.05%. As a result, colonies grew from the start of the culture, and grown 15 days after the start of the culture so that the colonies covered the entire plate surface.

[Comparative Example 16]
The same experiment as in Reference Example 26 was performed except that a PDA medium was prepared using distilled water instead of the potassium cinnamate aqueous solution. As a result, colonies grew rapidly from the start of the culture, and grown 7 days after the start of the culture so that the colonies covered the entire plate surface.
A summary of Reference Example 26 and Comparative Examples 15 and 16 is shown in Table 10. From these results, it was shown that the growth of carnation root rot pathogen can be effectively suppressed by containing potassium cinnamate which is the plant pathogen inhibitor of the present invention in the medium. The plant pathogenic fungus inhibitor of the present invention is higher in water solubility than cinnamic acid, so that a solution having a higher concentration than a saturated cinnamic acid aqueous solution can be prepared. By using such a high concentration solution, cinnamic acid It was found that the carnation root rot pathogen control effect is much stronger than when using the saturated aqueous solution.

<Preparation of sodium cinnamate powder>
Sodium hydroxide and cinnamic acid were gradually added and dissolved in 650 mL of distilled water at 90 ° C. to prepare an aqueous cinnamic acid solution having a concentration of 10%. Next, this solution was cooled to 25 ° C. and then allowed to stand at 4 ° C. for 12 hours to precipitate a white substance. This white substance was taken out, air-dried and then dried at 105 ° C. for 6 hours to obtain a white powder. Hereinafter, this white powder is referred to as white powder (B). As with the white powder (A), fluorescent X-ray analysis, infrared spectroscopic spectrum measurement, ion chromatogram measurement and cinnamic acid component were quantified to confirm that the white powder (B) was sodium cinnamate.

<Inhibition test of sodium cinnamate against pathogen of dollar spot disease>
[ Reference Example 27]
The experiment was conducted in the same manner as in Reference Example 2 except that the white powder (B) was used instead of the white powder (A) and the colony diameter was measured 3, 5, and 7 days after the start of the culture.
As a result, a small colony was generated 3 days after the start of the experiment, and then the colony gradually grew.

[ Reference Example 28]
The experiment was conducted in the same manner as in Reference Example 27 except that the sodium cinnamate concentration in the agar plate was 0.1%. Even after 7 days from the start of culture, no colonies were generated on the plate.

[Comparative Example 17]
Colonies were measured in the same manner as in Reference Example 27 except that the PDA medium was prepared using distilled water instead of the sodium cinnamate aqueous solution. As a result, colonies grew rapidly from the start of the culture, and grown 7 days after the start of the culture so that the colonies covered the entire plate surface.

A summary of Reference Examples 27 and 28 and Comparative Example 17 is shown in Table 11. From the result of Table 11, it was shown that the growth of dollar spot disease pathogen can be effectively suppressed by containing sodium cinnamate which is a plant pathogen inhibitor of the present invention in the medium. In particular, it has been found that when the concentration in the medium is 0.1%, the growth of dollar spot disease pathogens can be completely suppressed.

<Inhibition test of sodium cinnamate against large patch disease pathogen>
[ Reference Examples 29 and 30, Comparative Example 18]
Experiments similar to Reference Example 27, Reference Example 28, and Comparative Example 17 were performed using a large patch disease pathogen (Rhizoctonia solani AG2-2 LP) instead of the dollar spot disease pathogen ( Reference Example 29 and Reference Example 30 respectively). Comparative Example 18). The results are shown in Table 12. From these results, it was found that the growth of large patch disease pathogens can be completely inhibited by containing 0.05% or more of sodium cinnamate, which is a plant pathogen inhibitor of the present invention, in the medium.

<Inhibition test of sodium cinnamate against pathogen of brown patch disease>
[ Reference Examples 31 to 33, Comparative Example 19]
The same experiment as Reference Example 29, Reference Example 30 and Comparative Example 18 was conducted except that Brown patch disease pathogen (Rhizoctonia solani AG2-2IIIB) was used instead of large patch disease pathogen ( Reference Example 31, Reference 31 respectively) Example 32, Comparative Example 19). Further, an experiment similar to Reference Example 31 was conducted except that the concentration of the sodium cinnamate aqueous solution was 0.5% ( Reference Example 33). The results are shown in Table 13. From these results, it was found that the growth of brown patch disease pathogens can be suppressed by containing 0.05% or more of sodium cinnamate, which is a plant pathogen inhibitor of the present invention, in the medium. In particular, when the sodium hydroxide concentration was 0.5%, it was shown that the growth of the brown patch disease pathogen can be completely suppressed.

<Inhibition of large patch disease pathogens by mixture of cinnamic acid and weak acid salt>
[ Reference Example 34]
1.48 parts of cinnamic acid and 1 part of potassium hydrogen carbonate were mixed to prepare a composition (hereinafter abbreviated as composition A) which is a phytopathogenic fungus inhibitor of the present invention. 1.34 g of this composition A was dissolved in 1 liter of water to prepare an aqueous composition A solution of about 0.13%. A PDA medium was prepared using this 0.13% composition A aqueous solution, and a large patch disease pathogen (Rhizoctonia solani AG2-2 LP) was used instead of the dollar spot disease pathogen, and the colony diameter was measured from the beginning of the culture. The experiment was performed in the same manner as in Reference Example 1 except that the test was performed after 5 and 7 days.

[ Reference Examples 35 and 36, Comparative Example 20]
An experiment was conducted in the same manner as in Reference Example 34 except that a PDA medium was prepared using 0.065% Composition A aqueous solution, 0.013% Composition A aqueous solution or distilled water ( Reference Examples 35 and 36, respectively). Comparative Example 20). The results are shown in Table 14.
From the results of Table 14, the growth of large patch disease pathogens is effective by containing 0.013% (0.01% as potassium cinnamate) or more of composition A, which is a plant pathogen inhibitor of the present invention, in the medium. It was found that it can be suppressed. In particular, it was shown that by containing 0.065% or more of composition A (0.05% as potassium cinnamate) or more, the growth of large patch disease pathogens can be completely suppressed.

[ Reference Example 37]
<Brown patch disease pathogen control effect of cinnamic acid and weak acid salt mixture>
The experiment was conducted in the same manner as in Reference Example 34 except that the concentration of the aqueous composition A solution was 0.65%, and a brown patch disease pathogen (Rhizoctonia solani AG2-2IIIB) was used instead of the large patch disease pathogen.

[ Reference Examples 38 and 39, Comparative Example 21]
An experiment was conducted in the same manner as in Reference Example 37 except that a PDA medium was prepared using 0.13% Composition A aqueous solution, 0.065% Composition A aqueous solution or distilled water ( Reference Examples 38 and 39, respectively). Comparative Example 21). The results are shown in Table 15.
From these results, the growth of Brown patch disease pathogens is effectively achieved by containing 0.065% (0.05% as potassium cinnamate) or more of composition A, which is a plant pathogen inhibitor of the present invention, in the medium. It was found that it can be suppressed. In particular, it was shown that the growth of Brown patch disease pathogens can be completely suppressed by containing 0.65% of composition A (0.5% as potassium cinnamate).

<Inhibitory effect of a mixture of cinnamic acid and weak acid salt against pathogenic bacteria of Psium disease>
[ Reference Example 40]
Experiments were conducted in the same manner as in Reference Example 34, except that Pythium graminicola was used instead of the large patch disease pathogen, and the colony diameter was measured 2, 4, and 7 days after the start of culture.

[ Reference Examples 41 to 44, Comparative Example 22]
Reference except that PDA medium was prepared using 0.065% Composition A aqueous solution, 0.013% Composition A aqueous solution, 0.0065% Composition A aqueous solution, 0.0013% Composition A aqueous solution or distilled water. The experiment was performed in the same manner as in Example 40 ( Reference Examples 41 to 44 and Comparative Example 22 respectively). The results are shown in Table 16.
From these results, by containing 0.0013% (0.001% as potassium cinnamate) or more of the composition A, which is a phytopathogenic agent of the present invention, in the medium, the growth of Pisium disease pathogens is effectively achieved. It turned out that it can suppress. In particular, it was shown that by containing 0.0065% or more of composition A (0.005% as potassium cinnamate) or more, the growth of Pisium disease pathogens can be completely suppressed.

<Inhibition test of liquid fertilizer composition containing cinnamate potassium cinnamate (3-component system) against dollar spot disease pathogens>
[ Reference Example 45]
0.34 g of potassium nitrate and 0.08 g of diammonium hydrogen phosphate were dissolved in 400 mL of distilled water at 20 ° C. to prepare a liquid fertilizer base solution. The white powder (A) was dissolved in this liquid fertilizer base solution to prepare a liquid fertilizer composition containing potassium cinnamate.
A PDA medium having a potassium cinnamate concentration of 0.01% was prepared using the above-described potassium cinnamate-containing liquid fertilizer composition, and the colony diameter was measured 2, 4, and 8 days after the start of culture. The experiment was performed in the same manner as in Reference Example 1.

[ Reference Example 46, Comparative Example 23]
The experiment was conducted in the same manner as in Reference Example 45 except that the potassium cinnamate concentration in the PDA medium was 0.1% ( Reference Example 46) or 0% (Comparative Example 23). The results are shown in Table 17. From these results, it was shown that the growth of dollar spot disease pathogens can be effectively suppressed by preparing a culture medium using the liquid fertilizer composition containing potassium cinnamate, which is a plant pathogen inhibitor of the present invention. In particular, it was found that when the potassium cinnamate concentration in the medium is 0.1%, the growth of dollar spot disease pathogens can be completely suppressed.

<Suppression test for large patch disease pathogens of liquid fertilizer composition containing potassium cinnamate (4-component system)>
[ Reference Example 47]
A liquid fertilizer base solution was prepared by dissolving 0.19 g of potassium nitrate, 0.10 g of ammonium nitrate and 0.06 g of dipotassium hydrogen phosphate in 400 mL of distilled water at 20 ° C. The white powder (A) was dissolved in this liquid fertilizer base solution to prepare a liquid fertilizer composition of the present invention containing potassium cinnamate.
A PDA medium having a potassium cinnamate concentration of 0.01% was prepared using the above-described potassium cinnamate-containing liquid fertilizer composition, and the same procedure as in Reference Example 45 was used except that a large patch disease pathogen was used instead of the dollar spot disease pathogen. The experiment was conducted.

[ Reference Example 48, Comparative Example 24]
The experiment was conducted in the same manner as in Reference Example 47 except that the concentration of potassium cinnamate in the PDA medium was 0.1% ( Reference Example 48) or 0% (Comparative Example 24). The results are shown in Table 18. From these results, by using the liquid fertilizer composition containing potassium cinnamate, which is the plant pathogen inhibitor of the present invention, and setting the potassium cinnamate concentration in the medium to 0.1%, the growth of the large patch disease pathogen is completely achieved. It was shown that it can be suppressed.

[Reference Experimental Example 1]
<Solubility test of plant pathogen inhibitor in water>
Potassium cinnamate powder (white powder (A)) was added to 100 g of water at a water temperature of 20 ° C., and the time until the aqueous solution became transparent was measured while stirring with a stirrer. Similar experiments were performed for sodium cinnamate powder (white powder (B)) and cinnamic acid. The results are shown in Table 19.
From the results shown in Table 19, it was found that potassium cinnamate powder, which is a phytopathogenic fungus inhibitor of the present invention, was reliably dissolved in water at 20 ° C. up to about 35%, and sodium cinnamate powder was dissolved up to about 5%. In particular, potassium cinnamate powder was easily dissolved in a short time up to about 25%. From this, it became clear that the potassium cinnamate powder has high practicality when preparing the application liquid at the application site. On the other hand, cinnamic acid powder has low water solubility, making it difficult to prepare an aqueous solution.

According to the present invention, there is provided a phytopathogenic fungus inhibitor that has a higher solubility than cinnamic acid and can prepare a high-concentration aqueous solution. The plant pathogen inhibitor of the present invention contains a cinnamic acid salt, which is a natural product, as an active ingredient, has extremely high safety, and can inhibit only plant pathogens without adversely affecting the plant body.
Since the phytopathogenic fungus inhibitor of the present invention can easily prepare a high-concentration aqueous solution, it can not only prepare a high-concentration solution excellent in storage stability but also has excellent transport efficiency. In addition, taking advantage of its high water solubility, it can be transported as a powder or granule, and can easily prepare phytopathogenic fungus inhibitors at a concentration according to the purpose of application, contributing to efficient vegetation management. it can.

Claims (8)

A basidiomycete inhibitor comprising a mixture of cinnamic acid and an alkali metal compound or cinnamic acid salt. The basidiomycete inhibitor according to claim 1, wherein the cinnamate contains at least potassium cinnamate or sodium cinnamate. The basidiomycete inhibitor according to claim 1, wherein the alkali metal compound is at least one selected from the group consisting of potassium carbonate, sodium carbonate, potassium hydrogen carbonate and sodium hydrogen carbonate. The basidiomycete is, Basidiomycetes inhibitor according to claim 1 is a flow Eariringu disease pathogenic bacterium. The basidiomycete-suppressing agent according to claim 4, wherein the fairy ring disease pathogenic bacterium is Chibidokoritake or Hidadokoritake. The basidiomycete inhibitor according to claim 1, which is for turfgrass. The basidiomycete inhibitor according to claim 6 , wherein the turfgrass is creeping bentgrass. A fertilizer composition comprising the basidiomycete inhibitor according to any one of claims 1 to 7 .

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